| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,822,088 | C→T | 7.4% | S465S (TCC→TCT) | norV → | flavorubredoxin oxidoreductase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,822,088 | 0 | C | T | 7.4% | 204.8 / 6.1 | 67 | S465S (TCC→TCT) | norV | flavorubredoxin oxidoreductase |
| Reads supporting (aligned to +/- strand): ref base C (21/41); new base T (3/2); total (24/43) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.41e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.57e-01 | |||||||||||
GCAGGACGTTGCGCCAGGAACGCCGTGGAGTGAAGTCCCGGATAACTTCCTCTGCCCGGAATGCTCCCTCGGCAAAGACGTCTTTGAAGAACTGGCATCGGAGGCAAAATGAGTAACGGCATTGTGATCATCGGTTC > minE/1822022‑1822158 | gCAGGACGTTGCGCCAGGAACGCCGTGGAGTGAAGTCCCGGATAACTTCCTCTGCCCGGAATGCTCCCTCg > 1:1037953/1‑71 (MQ=255)gCAGGACGTTGCGCCAGGAACGCCGTGGAGTGAAGTCCCGGATAACTTCCTCTGCCCGGAATGCTCCCTCg > 1:986216/1‑71 (MQ=255)gCAGGACGTTGCGCCAGGAACGCCGTGGAGTGAAGTCCCGGATAACTTCCTCTGCCCGGAATGCTCCCTCg > 1:79652/1‑71 (MQ=255) ggACGTTGCGCCAGGAACGCCGTGGAGTGAAGTCCCGGATAACTTCCTCTGCCCGGAATGCTCCCTCGGCa < 1:1577599/71‑1 (MQ=255) ggACGTTGCGCCAGGAACGCCGTGGAGTGAAGTCCCGGATAACTTCCTCTGCCCGGAATGCTCCCTCGGCa < 1:15510/71‑1 (MQ=255) ggACGTTGCGCCAGGAACGCCGTGGAGTGAAGTCCCGGATAACTTCCTCTGCCCGGAATGCTCCCTCGGCa < 1:1378293/71‑1 (MQ=255) aCGTTGCGCCAGGAACGCCGTGGAGTGAAGTCCCTGATAACTTCCTCTGCCCGGAATGCTCCCTCGGCaaa < 1:1846075/71‑1 (MQ=255) aCGTTGCGCCAGGAACGCCGTGGAGTGAAGTCCCGGATAACTTCCTCTGCCCGGAATGCTCCCTCGGCaaa < 1:3443201/71‑1 (MQ=255) aCGTTGCGCCAGGAACGCCGTGGAGTGAAGTCCCGGATAACTTCCTCTGCCCGGAATGCTCCCTCGGCaaa < 1:3507663/71‑1 (MQ=255) aCGTTGCGCCAGGAACGCCGTGGAGTGAAGTCCCGGATAACTTCCTCTGCCCGGAATGCTCCCTCGGCaaa < 1:593504/71‑1 (MQ=255) aCGTTGCGCCAGGAACGCCGTGGAGTGAAGTCCCGGATAACTTCCTCTGCCCGGAATGCTCCCTCGGCaaa < 1:3094061/71‑1 (MQ=255) tGCGCCAGGAACGCCGTGGAGTGAAGTCCCGGATAACTTCCTCTGCCCGGAATGCTCCCTCGGCAAAGACg > 1:2045001/1‑71 (MQ=255) tGCGCCAGGAACGCCGTGGAGTGAAGTCCCGGATAACTTACTCTGCCCGGAATGCTCCCTCGGCAAAGACg > 1:915029/1‑71 (MQ=255) cAGGAACGCCGTGGAGTGAAGTCCCGGATAACTTCCTCTGCCCGGAATGCTCCCTCGGCAAAGACGTCttt > 1:3175304/1‑71 (MQ=255) gCCGTGGAGTGAAGTCCCGGATAACTTCCTCTGCCCGGAATGCTCCCTCGGCAAAGACGTCTTTGAAGAAc < 1:204297/71‑1 (MQ=255) cGTGGAGTGAAGTCCCGGATAACTTCCTGTGCCCGGAATGCTCTCTCGGCAAAGACGTCttt < 1:3934898/62‑1 (MQ=255) tgtaGTGAAGTCCCGGATAACTTCCTGTGCCCGGAATGCTCTCTCGGCAAAGACGTCTTTga > 1:4098216/4‑62 (MQ=255) tGGAGTGAAGTCCCGGATAACTTCCTGTGCCCGGAATGCTCTCTCGGCAAAGACGTCTTTga > 1:1682524/1‑62 (MQ=255) tGGAGTGAAGTCCCGGATAACTTCCTGTGCCCGGAATGCTCTCTCGGCAAAGACGTCTTTga > 1:401771/1‑62 (MQ=255) tGAAGTCCCGGATAACTTCCTCTGCCCGGAATGCTCCCTCGGCAAAGACGTCTTTGAAGAACTGGCATCgg < 1:428721/71‑1 (MQ=255) gAAGTCCCGGATAACTTCCTCTGCCCGGAATGCTCCCTCGGCAAAGACGTCtt > 1:3798422/1‑53 (MQ=255) gAAGTCCCGGATAACTTCCTCTGCCCGGAATGCTCCCTCGGCAAAGACGTCtt > 1:1187813/1‑53 (MQ=255) aaGTCCCGGATAACTTCCTCTGCCCGGAATGCTCCCTCGGCAAAGACGTCTTTGAAGAACTGGCATCGGa < 1:1948168/70‑1 (MQ=255) aaGTCCCGGATAACTTCCTCTGCCCGGAATGCTCCCTCGGCAAAGACGTCTTTGAAGAACTGGCATCGGa < 1:704330/70‑1 (MQ=255) aaGTCCCGGATAACTTCCTCTGCCCGGAATGCTCCCTCGGCAAAGACGTCTTTGAAGAACTGGCATCGGAg > 1:3343282/1‑71 (MQ=255) aaGTCCCGGATAACTTCCTCTGCCCGGAATGCTCCCTCGGCAAAGACGTCTTTGAAGAACTGGCATCGGAg > 1:3231385/1‑71 (MQ=255) tCCCGGATAACTTCCTCTGCCCGGAATGCTCCCTCGGCAAAGACGTc < 1:2881727/47‑1 (MQ=255) tCCCGGATAACTTCCTCTGCCCGGAATGCTCCCTCGGCAAAGACGTc < 1:1798427/47‑1 (MQ=255) tCCCGGATAACTTCCTCTGCCCGGAATGCTCCCTCGGCAAAGACGTc < 1:508133/47‑1 (MQ=255) tCCCGGATAACTTCCTCTGCCCGGAATGCTCCCTCGGCAAAGACGTc < 1:1522549/47‑1 (MQ=255) tCCCGGATAACTTCCTCTGCCCGGAATGCTCCCTCGGCAAAGACGTc < 1:1088762/47‑1 (MQ=255) tCCCGGATAACTTCCTCTGCCCGGAATGCTCCCTCGGCAAAGACGTCTTTg < 1:996357/51‑1 (MQ=255) tCCCGGATAACTTCCTCTGCCCGGAATGCTCCCTCGGCAAAGACGTCTTTg < 1:2497866/51‑1 (MQ=255) tCCCGGATAACTTCCTCTGCCCGGAATGCTCCCTCGGCAAAGACGTCTTTg < 1:2087905/51‑1 (MQ=255) tCCCGGATAACTTCCTCTGCCCGGAATGCTCCCTCGGCAAAGACGTCTTTg < 1:2295438/51‑1 (MQ=255) tCCCGGATAACTTCCTCTGCCAGGAATGCTCCCTCGGCAAAGACGTc < 1:1496566/47‑1 (MQ=255) ccGGATAACTTCCTCTGCCCGGAATGCTCCCTCGGCAAAGACGTCTTTgaaga < 1:2764190/53‑1 (MQ=255) ccGGATAACTTCCTCTGCCCGGAATGCTCCCTCGGCAAAGACGTCTTTgaaga < 1:1527518/53‑1 (MQ=255) ccGGATAACTTCCTCTGCCAGGAATGCTCCCTCGGCAAAGACGTCTTTgaaga < 1:1973356/53‑1 (MQ=255) tAACTTCCTCTGCCCGGAATGCTCCCTCGGCAAAGACGTCTTTGAAGAACTGGCATCGGAGGCAAAATGAg > 1:3135539/1‑71 (MQ=255) aaCTTCCTCTGCCCGGAATGCTCCCTCGGCAAAGACGTCttt > 1:4023595/1‑42 (MQ=255) aaCTTCCTCTGCCCGGAATGCTCCCTCGGCAAAGACGTCTTTGAAGAACTGGCATCGGAGGCAAAATGAg > 1:714312/1‑70 (MQ=255) aaCTTCCTCTGCCCGGAATGCTCCCTCGGCAAAGACGTCTTTGAAGAACTGGCATCGGAGGCAAAATGAg > 1:3294313/1‑70 (MQ=255) aaCTTCCTCTGCCCGGAATGCTCCCTCGGCAAAGACGTCTTTGAAGAACTGGCATCGGAGGCAAAATGAg > 1:2923017/1‑70 (MQ=255) cTTCCTCTGCCCGGAATGCTCCCTCGGCAAAGACGTCTTTGAAGAACTGGCATCGGAGGCAAAATGAGt < 1:296617/69‑1 (MQ=255) cTTCCTCTGCCCGGAATGCTCCCTCGGCAAAGACGTCTTTGAAGAACTGGCATCGGAGGCAAAATGAGt < 1:1553436/69‑1 (MQ=255) tCCTCTGCCCGGAATGCTCCCTCGGCAAAGACGTCTTTGAAGAACTGGCATCGGAGGCAAAATGAGt < 1:612268/67‑1 (MQ=255) tCCTCTGCCCGGAATGCTCCCTCGGCAAAGACGTCTTTGAAGAACTGGCATCGGAGGCAAAATGAGTaa > 1:1279720/1‑69 (MQ=255) tCCTCTGCCCGGAATGCTCCATCGGCAAAGACGTCTTTGAAGAACTGGCATCGGAGGCAAAATGAGTaa > 1:3305312/1‑69 (MQ=255) ccTCTGCCCGGAATGCTCCCTCGGCAAAGACGTCTTTGAAGAACTGGCATCGGAGGCAAAATGAGTAACgg > 1:112670/1‑71 (MQ=255) cccGGAATGCTCCCTCGGCAAAGACGTCTTTGAAGAACTGGCATGGGAGGCAAAATGAGt > 1:1807656/1‑60 (MQ=255) cccGGAATGCTCCCTCGGCAAAGACGTCTTTGAAGAACTGGCATCGGAGGCAAAATGAGTAACGGCAtt < 1:3719068/69‑1 (MQ=255) cccGGAATGCTCCCTCGGCAAAGACGTCTTTGAAGAACTGGCATCGGAGGCAAAATGAGTAACGGCATtgt > 1:3371230/1‑71 (MQ=255) cccGGAATGCTCCCTCGGCAAAGACGTCTTTGAAGAACTGGCATCGGAGGCAAAATGAGTAACGGCATtgt > 1:973985/1‑71 (MQ=255) ccGGAATGCTCCCTCGGCAAAGACGTCTTTGAAGAACTGGCATCGGAGGCAAAATGAGTAACGGCATtgtg < 1:652644/71‑1 (MQ=255) ccGGAATGCTCCCTCGGCAAAGACGTCTTTGAAGAACTGGCATCGGAGGCAAAATGAGTAACGGCATtgtg < 1:3765357/71‑1 (MQ=255) ccGGAATGCTCCCTCGGCAAAGACGTCTTTGAAGAACTGGCATCGGAGGCAAAATGAGTAACGGCATtgtg < 1:3749601/71‑1 (MQ=255) ggAATGCTCCCTCGGCAAAGACGTCTTTGAAGAACTGGCATCGGAGGCAAAATGAGTAACGGCATTGTGat < 1:1594890/71‑1 (MQ=255) ggAATGCTCCCTCGGCAAAGACGTCTTTGAAGAACTGGCATCGGAGGCAAAATGAGTAACGGCATTGTGat < 1:2026110/71‑1 (MQ=255) ggAATGCTCCCTCGGCAAAGACGTCTTTGAAGAACTGGCATCGGAGGCAAAATGAGTAACGGCATTGTGat < 1:2343064/71‑1 (MQ=255) ggAATGCTCCCTCGGCAAAGACGTCTTTGAAGAACTGGCATCGGAGGCAAAATGAGTAACGGCATTGTGat < 1:3124507/71‑1 (MQ=255) ggAATGCTCCCTCGGCAAAGACGTCTTTGAAGAACTGGCATCGGAGGCAAAATGAGTAACGGCATTGTGat < 1:1099633/71‑1 (MQ=255) ggAATGCTCCCTCGGCAAAGACGTCTTTGAAGAACTGGCATCGGAGGCAAAATGAGTAACGGCATTGTGat < 1:1055708/71‑1 (MQ=255) aaTGCTCCCTCGGCAAAGACGTCTTTGAAGAACTGGCATCGGAGGCAAAATGAGTAACGGCATTGTGatca < 1:2195509/71‑1 (MQ=255) aTGCTCTCTCGGCAAAGACGTCTTTGACGAACTGGCATCGGAGGCAAAATGAGCAACGGCATTGTGatca < 1:3134739/70‑1 (MQ=255) tCCCTCGGCAAAGACGTCTTTGAAGAACTGGCATCGGAGGCaa < 1:2115872/43‑1 (MQ=255) tCCCTCGGCAAAGACGTCTTTGAAGAACTGGCATCGGAGGCaa < 1:3204561/43‑1 (MQ=255) ccTCGGCAAAGACGTCTTTGAAGAACTGGCATCGGAGGCaa < 1:3273655/41‑1 (MQ=255) ccTCGGCAAAGACGTCTTTGAAGAACTGGCATCGGAGGCAAAATGAGTAACGGCATTGTGATCATCGGTTc > 1:4029227/1‑71 (MQ=255) | GCAGGACGTTGCGCCAGGAACGCCGTGGAGTGAAGTCCCGGATAACTTCCTCTGCCCGGAATGCTCCCTCGGCAAAGACGTCTTTGAAGAACTGGCATCGGAGGCAAAATGAGTAACGGCATTGTGATCATCGGTTC > minE/1822022‑1822158 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |