| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,827,932 | C→T | 57.1% | A392V (GCG→GTG) | mutS → | methyl‑directed mismatch repair protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,827,932 | 0 | C | T | 57.1% | 13.5 / 63.5 | 49 | A392V (GCG→GTG) | mutS | methyl‑directed mismatch repair protein |
| Reads supporting (aligned to +/- strand): ref base C (10/11); new base T (15/13); total (25/24) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.76e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.74e-01 | |||||||||||
CACGCTTTCCAGCAACTGCCGGAGCTGCGTGCGCAGTTAGAAACTGTCGATAGTGCACCGGTACAGGCGCTACGTGAGAAGATGGGCGAGTTTGCCGAGCTGCGCGATCTGCTGGAGCGAGCAATCATCGA > minE/1827865‑1827995 | cACGCTTTCCAGCAACTGCCGGAGCTGCGTGCGCAGTTAGAAACTGTCGATAGTGCACCGGTACAGGCGCt < 1:1822065/71‑1 (MQ=255) cGCTTTCCAGCAACTGCCGGAGCTGCGTGCGCAGTTAGAAACTGTCGATAGTGCACCGGTACAGGTGCTAc > 1:1328218/1‑71 (MQ=255) cGCTTTCCAGCAACTGCCGGAGCTGCGTGCGCAGTTAGAAACTGTCGATAGTGCACCGGTACAGGTGCTAc > 1:1468504/1‑71 (MQ=255) cGCTTTCCAGCAACTGCCGGAGCTGCGTGCGCAGTTAGAAACTGTCGATAGTGCACCGGTACAGGTGCTAc > 1:2558531/1‑71 (MQ=255) cTTTCCAGCAACTGCCGGAGCTGCGTGCGCAGTTAGAAACTGTCGATAGTGCACCGGTACAGGCGCTACGt > 1:4012965/1‑71 (MQ=255) ttCCAGCAACTGCCGGAGCTGCGTGCGCAGTTAGAAACTGTCGATAGTGCACCGGTACAGGCGCTACGt > 1:2640361/1‑69 (MQ=255) cAGCAACTGCCGGAGCTGCGTGCGCAGTTAGAAACTGTCGATAGTGCACCGGTACAGGTGCTACGTGagaa < 1:1953439/71‑1 (MQ=255) aGCAACTGCCGGAGCTGCGTGCGCAGTTAGAAACTGTCGATAGTGCACCGGTACAGGTGCTACGTGagaag < 1:2932183/71‑1 (MQ=255) aGCAACTGCCGGAGCTGCGTGCGCAGTTAGAAACTGTCGATAGTGCACCGGTACAGGTGCTACGTGagaag < 1:3782764/71‑1 (MQ=255) aGCAACTGCCGGAGCTGCGTGCGCAGTTAGAAACTGTCGATAGTGCACCGGTACAGGTGCTACGTGagaag < 1:319895/71‑1 (MQ=255) aGCAACTGCCGGAGCTGCGTGCGCAGTTAGAAACTGTCGATAGTGCACCGGTACAGGCGCTACGTGagaag < 1:4246975/71‑1 (MQ=255) aGCAACTGCCGGAGCTGCGTGCGCAGTTAGAAACTGTCGATAGTGCACCGGTACAGGCGCTACGTGagaag < 1:4042574/71‑1 (MQ=255) cTGCCGGAGCTGCGTGCGCAGTTAGAAACTGTCGATAGTGCACCGGTACAGGCGCt < 1:580755/56‑1 (MQ=255) gCCGGAGCTGCGTGCGCAGTTAGAAACTGTCGATAGTGCACCGGTACAGGCGCTACGTGAGAAGATGGGc > 1:3874437/1‑70 (MQ=255) ggAGCTGCGTGCGCAGTTAGAAACTGTCGATAGTGCACCGGTACAGGTGCTACGTGAGAAGATGGGCGAGt > 1:1241609/1‑71 (MQ=255) ggAGCTGCGTGCGCAGTTAGAAACTGTCGATAGTGCACCGGTACAGGTGCTACGTGAGAAGATGGGCGAGt > 1:3584804/1‑71 (MQ=255) gcgtgcgCAGTTAGAAACTGTCGATAGTGCACCGGTACAGGCGCTACGTGAGAAGATGGGCGAGTTTGcc > 1:497810/1‑70 (MQ=255) gtgcgCAGTTAGAAACTGTCGATAGTGCACCGGTACAGGCGCTACGTGAGAAGATGGGCGAGTTTGCCGAg < 1:3148819/71‑1 (MQ=255) tgcgCAGTTAGAAACTGTCGATAGTGCACCGGTACAGGCGCTACGTGAGAAGATGGGCGAGTTTGCCGAg < 1:3177132/70‑1 (MQ=255) cgcAGTTAGAAACTGTCGATAGTGCACCGGTACAGGTGCTACGTGAGAAGATGGGCGAGTTTGCCGAGCt < 1:2296863/70‑1 (MQ=255) aGTTAGAAACTGTCGATAGTGCACCGGTACAGGCGCTACGTGagaa < 1:685715/46‑1 (MQ=255) aGTTAGAAACTGTCGATAGTGCACCGGTACAGGCGCTACGTGagaa < 1:2346200/46‑1 (MQ=255) aGTTAGAAACTGTCGATAGTGCACCGGTACAGGCGCTACGTGagaa < 1:2013691/46‑1 (MQ=255) ttAGAAACTGTCGATAGTGCACCGGTACAGGCGCTACGTGagaaga > 1:2931519/1‑46 (MQ=255) aGAAACTGTCGATAGTGCACCGGTACAGGTGCTACGTGAGAAGATGGGCGAGTTTGCCGAGCTGCGCGATc > 1:2615215/1‑71 (MQ=255) aGAAACTGTCGATAGTGCACCGGTACAGGTGCTACGTGAGAAGATGGGCGAGTTTGCCGAGCTGCGCGATc > 1:2184154/1‑71 (MQ=255) gAAACTGTCGATAGTGCACCGGTACAGGTGCTACGTGAGAAGATGGGCGAGTTTGCCGAGCt > 1:862073/1‑62 (MQ=255) gAAACTGTCGATAGTGCACCGGTACAGGTGCTACGTGAGAAGATGGGCGAGTTTGCCGAGCTg > 1:2836989/1‑63 (MQ=255) gAAACTGTCGATAGTGCACCGGTACAGGTGCTACGTGAGAAGATGGGCGAGTTTGCCGAGCTg > 1:1040687/1‑63 (MQ=255) gAAACTGTCGATAGTGCACCGGTACAGGTGCTACGTGAGAAGATGGGCGAGTTTGCCGAGCTg > 1:453191/1‑63 (MQ=255) gAAACTGTCGATAGTGCACCGGTACAGGTGCTACGTGAGAAGATGGGCGAGTTTGCCGAGCTg > 1:3997992/1‑63 (MQ=255) cTGTCGATAGTGCACCGGTACAGGTGCTACGTGAGAAGATGGGCGAGTTTGCCGAGCTGCGCGATctgctg > 1:2467489/1‑71 (MQ=255) cTGTCGATAGTGCACCGGTACAGGTGCTACGTGAGAAGATGGGCGAGTTTGCCGAGCTGCGCGATctgct > 1:2203720/1‑70 (MQ=255) gTCGATAGTGCACCGGTACAGGTGCTACGTGAGAAGATGGGCGAGTTTGCCGAGCTGCGCGATctgctg > 1:3524046/1‑69 (MQ=255) gagaGTGCACCGGTACAGGCGCTACGTGAGAAGATGGGCGAGTTTGCCGAGCTGCGCGATCTGCTGgagc > 1:1817857/4‑70 (MQ=255) gagaGTGCACCGGTACAGGCGCTACGTGAGAAGATGGGCGAGTTTGCCGAGCTGCGCGATCTGCTGgagc > 1:1822706/4‑70 (MQ=255) cGGTACAGGTGCTACGTGAGAAGATGGGCGAGTTTGCCGAGCTGCGCGATCTGCTGGAGCGAGCAatcatc < 1:268817/71‑1 (MQ=255) cGGTACAGGTGCTACGTGAGAAGATGGGCGAGTTTGCCGAGCTGCGCGATCTGCTGGAGCGAGCAatcatc < 1:706385/71‑1 (MQ=255) cGGTACAGGTGCTACGTGAGAAGATGGGCGAGTTTGCCGAGCTGCGCGATCTGCTGGAGCGAGCAatcatc < 1:639204/71‑1 (MQ=255) gTACAGGTGCTACGTGAGAAGATGGGCGAGTTTGCCGAGCTGCGCGATCTGCTGGAGCGAGCAATCATCGa < 1:1052021/71‑1 (MQ=255) gTACAGGTGCTACGTGAGAAGATGGGCGAGTTTGCCGAGCTGCGCGATCTGCTGGAGCGAGCAATCATCGa < 1:2516060/71‑1 (MQ=255) gTACAGGCGCTACGTGAGAAGATGGGCGAGTTTGCCGAGCTGCGCGATCTGCTGGAGCGAGCAatcat > 1:2717450/1‑68 (MQ=255) gTACAGGCGCTACGTGAGAAGATGGGCGAGTTTGCCGAGCTGCGCGATCTGCTGGAGCGAGCAatcat > 1:2564767/1‑68 (MQ=255) gTACAGGCGCTACGTGAGAAGATGGGCGAGTTTGCCGAGCTGCGCGATCTGCTGGAGCGAGCAatcat > 1:3171226/1‑68 (MQ=255) tACAGGTGCTACGTGAGAAGATGGGCGAGTTTGCCGAGCTGcgcg < 1:615488/45‑1 (MQ=255) cAGGTGCTACGTGAGAAGATGGGCGAGTTTGCCGAGCTGcgcg < 1:2890752/43‑1 (MQ=255) aGGTGCTACGTGAGAAGATGGGCGAGTTTGCCGAGCTGCGCGATCTGCTGGAGCGAGCAATCATCGa < 1:2085140/67‑1 (MQ=255) ggCGCTACGTGAGAAGATGGGCGAGTTTGCCGAGCTGCGCGATCTGCTGGAGCGAGCa < 1:458315/58‑1 (MQ=255) ggCGCTACGTGAGAAGATGGGCGAGTTTGCCGAGCTGCGCGATCTGCTGGAGCGAGCa < 1:847294/58‑1 (MQ=255) cgcTACGTGAGAAGATGGGCGAGTTTGCCGAGCTGCGCGATCTGCTGGAGCGAGCAat > 1:2791998/1‑58 (MQ=255) cgcTACGTGAGAAGATGGGCGAGTTTGCCGAGCTGCGCGATCTGCTGGAGCGAGCAat > 1:3817463/1‑58 (MQ=255) cgcTACGTGAGAAGATGGGCGAGTTTGCCGAGCTGCGCGATCTGCTGGAGCGAGCAat > 1:3632370/1‑58 (MQ=255) cgcTACGTGAGAAGATGGGCGAGTTTGCCGAGCTGCGCGATCTGCTGGAGCGAGCAat > 1:3193417/1‑58 (MQ=255) | CACGCTTTCCAGCAACTGCCGGAGCTGCGTGCGCAGTTAGAAACTGTCGATAGTGCACCGGTACAGGCGCTACGTGAGAAGATGGGCGAGTTTGCCGAGCTGCGCGATCTGCTGGAGCGAGCAATCATCGA > minE/1827865‑1827995 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |