| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,834,094 | A→G | 33.3% | D65G (GAT→GGT) | ygbM → | conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,834,094 | 0 | A | G | 33.3% | 72.7 / 58.3 | 63 | D65G (GAT→GGT) | ygbM | conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base A (22/20); new base G (8/13); total (30/33) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.23e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CCTGCAAATCCAAAAGCAACTGGAGCAAAACCATCTGACACTGGCGCTGTTCAATACCGCTCCCGGAGATATTAATGCGGGGGAATGGGGATTATCCGCCCTTCCCGGACGTGAGCACGAAGCACACGCAGACA > minE/1834026‑1834159 | ccTGCAAATCCAAAAGCAACTGGAGCAAAACCATCTGACACTGGCGCTGTTCAATACCGCTCCCGGAGgta < 1:1889291/71‑1 (MQ=255)ccTGCAAATCCAAAAGCAACTGGAGCAAAACCATCTGACACTCGCGCTGTGCAATACCGCTCCCGGAGgta < 1:1995791/71‑1 (MQ=255) tGCAAATCCAAAAGCAACTGGAGCAAAACCATCTGACACTGGCGCTGTTCAATACCGCTCCCGGAGgtat > 1:1703342/1‑70 (MQ=255) tGCAAATCCAAAAGCAACTGGAGCAAAACCATCTGACACTGGCGCTGTTCAATACCGCTCCCGGAGatat > 1:3992672/1‑70 (MQ=255) tGCAAATCCAAAAGCAACTGGAGCAAAACCATCTGACACTGGCGCTGTTCAATACCGCTCCCGGAGatat > 1:2838729/1‑70 (MQ=255) gCAAATCCAAAAGCAACTGGAGCAAAACCATCTGACACTGGCGCTGTTCAATACCGCTCCCGGAGATAtt > 1:19860/1‑70 (MQ=255) cAAATCCAAAAGCAACTGGAGCAAAACCATCTGACACTGGCGCTGTTCAATACCGCTCCCGGAGGTAtt < 1:1268010/69‑1 (MQ=255) cAAATCCAAAAGCAACTGGAGCAAAACCATCTGACACTGGCGCTGTTCAATACCGCTCCCGGAGATAtt < 1:978731/69‑1 (MQ=255) cAAATCCAAAAGCAACTGGAGCAAAACCATCTGACACTGGCGCTGTTCAATACCGCTCCCGGAGATAtt < 1:1857101/69‑1 (MQ=255) cAAATCCAAAAGCAAATGGAGCAAAACCATCTGAGACTGGCGCTGTTCAATACCGCTCCCGGAGATAtt < 1:102885/69‑1 (MQ=255) ccAAAAGCAACTGGAGCAAAACCATCTGACACTGGCGCTGTTCAATACCGCTCCCGgaga > 1:176224/1‑60 (MQ=255) ccAAAAGCAACTGGAGCAAAACCATCTGACACTGGCGCTGTTCAATACCGCTCCCGGAGGTATTAATGCgg > 1:3844107/1‑71 (MQ=255) cAAAAGCAACTGGAGCAAAACCATCTGACACTGGCGCTGTTCAATACCGCTCCCGGAGATATTAATGCggg > 1:3050709/1‑71 (MQ=255) cAAAAGCAACTGGAGCAAAACCATCTGACACTGGCGCTGTTCAATACCGCTCCCGGAGATATTAATGCggg > 1:3242364/1‑71 (MQ=255) aaaGCAACTGGAGCAAAACCATCTGACACTGGCGCTGTTCAATACCGCTCCCGGAGATATTAATGCggggg < 1:897529/71‑1 (MQ=255) aaGCAACTGGAGCAAAACCATCTGACACTGGCGCTGTTCAATACCGCTCCCGGAGATATTAATGCggggg > 1:60980/1‑70 (MQ=255) aGCAACTGGAGCAAAACCATCTGACACTGGCGCTGTTCAATACCGCTCCCGGAGGTATTAATGCgg > 1:1764193/1‑66 (MQ=255) aGCAACTGGAGCAAAACCATCTGACACTGGCGCTGTTCAATACCGCTCCCGGAGGTATTAATGCgg > 1:3502976/1‑66 (MQ=255) aGCAACTGGAGCAAAACCATCTGACACTGGCGCTGTTCAATACCGCTCCCGGAGATATTAATGCGGGGTaa > 1:2696666/1‑71 (MQ=255) cTGGAGCAAAACCATCTGACACTGGCGCTGTTCAATACCGCTCCCGGAGGTATTaa < 1:3562751/56‑1 (MQ=255) cTGGAGCAAAACCATCTGACACTGGCGCTGTTCAATACCGCTCCCGGAGGTATTaa < 1:1141773/56‑1 (MQ=255) cTGGAGCAAAACCATCTGACACTGGCGCTGTTCAATACCGCTCCCGGAGGTATTaa < 1:699248/56‑1 (MQ=255) cTGGAGCAAAACCATCTGACACTGGCGCTGTTCAATACCGCTCCCGGAGGTATTaa < 1:611859/56‑1 (MQ=255) aGCAAAACCATCTGACACTGGCGCTGTTCAATACCGCTCCCGGAGATATTAATGCGGGGGAATGGGGATTa < 1:1911740/71‑1 (MQ=255) aGCAAAACCATCTGACACTGGCGCTGTTCAATACCGCTCCCGGAGATATTAATGCGGGGGAATGGGGATTa < 1:2036527/71‑1 (MQ=255) aGCAAAACCATCTGACACTGGCGCAGTTCAATACCGCTCCCGGAGATATTAATGCGGGGGAATGGGGATTa < 1:481653/71‑1 (MQ=255) gCAAAACCATCTGACACTGGCGCTGTTCAATACCGCTCCCGGAGGTATTAATGCGGGGGAATGGGGa < 1:2027651/67‑1 (MQ=255) gCAAAACCATCTGACACTGGCGCTGTTCAATACCGCTCCCGGAGGTATTAATGCGGGGGAATGGGGa < 1:1535504/67‑1 (MQ=255) gCAAAACCATCTGACACTGGCGCTGTTCAATACCGCTCCCGGAGGTATTAATGCGGGGGAATGGGGa < 1:4169535/67‑1 (MQ=255) gCAAAACCATCTGACACTGGCGCTGTTCAATACCGCTCCCGGAGATATTAATGCGGGGGAATGGGGATTAt < 1:694394/71‑1 (MQ=255) aaaaCCATCTGACACTGGCGCTGTTCAATACCGCTCCCGGAGGTATTAATGCGGGGGAATGGGGATTATcc < 1:3464214/71‑1 (MQ=255) aCCATCTGACACTGGCGCTGTTCAATACCGCTCCCGGAGGTATTAATGCg > 1:2365559/1‑50 (MQ=255) aTCTGACACTGGCGCTGTTCAATACCGCTCCCGGAGATATTAATg < 1:3889604/45‑1 (MQ=255) aTCTGACACTGGCGCTGTTCAATACCGCTCCCGGAGATATTAATg < 1:3702437/45‑1 (MQ=255) aTCTGACACTGGCGCTGTTCAATACCGCTCCCGGAGATATTAATg < 1:1137914/45‑1 (MQ=255) cTGACACTGGCGCTGTTCAATACCGCTCCCGGAGATATTAATGCGGGGGaa > 1:2398858/1‑51 (MQ=255) tGACACTGGCGCTGTTCAATACCGCTCCCGGAGATATTAATGCGGGGGAATGGGGATTATCCGCCCTTccc > 1:1801727/1‑71 (MQ=255) tGACACTGGCGCTGTTCAATACCGCTCCCGGAGATATTAATGCGGGGGAATGGGGATTATCCGCCCTTccc > 1:3112606/1‑71 (MQ=255) tGACACTGGCGCTGTTCAATACCGCTCCCGGAGATATTAATGCGGGGGAATGGGGATTATCCGCCCTTcc > 1:3161035/1‑70 (MQ=255) acacTGGCGCTGTTCAATACCGCTCCCGGAGGTATTAATGCGGGGGAATGGGGATTATCCGCACTTcc < 1:23513/68‑1 (MQ=255) acacTGGCGCTGTTCAATACCGCTCCCGGAGATATTAATGCGGGGGAATGGGGATTATCCGCCCTTcc < 1:3549487/68‑1 (MQ=255) acacTGGCGCTGTTCAATACCGCTCCCGGAGATATTAATGCGGGGGAATGGGGATTATCCGCCCTTCCCg < 1:1406020/70‑1 (MQ=255) acacTGGCGCTGTTCAATACCGCTCCCGGAGATATTAATGCGGGGGAATGGGGATTATCCGCCCTTCCCg < 1:1358254/70‑1 (MQ=255) tcTGGCGCTGTTCAATACCGCTCCCGGAGGTATTAATGCGGGGGAATGGGGATTATc < 1:1877379/56‑1 (MQ=40) gcgcTGTTCAATACCGCTCCCGGAGGTATTAATGCGGGGGAATGGGGATTATCCGCCCTTCCCGGACGt < 1:2791910/69‑1 (MQ=255) gcgcTGTTCAATACCGCTCCCGGAGGTATTAATGCGGGGGAATGGGGATTATCCGCCCTTCCCGGACGt > 1:3223607/1‑69 (MQ=255) gcgcTGTTCAATACCGCTCCCGGAGGTATTAATGCGGGGGAATGGGGATTATCCGCCCTTCCCGGACGt > 1:2363006/1‑69 (MQ=255) gTTCAATACCGCTCCCGGAGATATTAATGCGGGGGAATGGGGATTATCCg < 1:2637958/50‑1 (MQ=255) gTTCAATACCGCTCCCGGAGATATTAATGCGGGGGAATGGGGATTATCCGCCCTTCCCGGACGTGAGCAc > 1:3323195/1‑70 (MQ=255) cAATACCGCTCCCGGAGATATTAATGCGGGGGAATGGGGATTATCCGCCCTTCCCGGACGTGAGCACGAAg > 1:401933/1‑71 (MQ=255) tACCGCTCCCGGAGATATTAATGCGGGGGAATGGGGATTATCCGCCCTTCCCGGACGTGAGCACGAAGcac < 1:3688205/71‑1 (MQ=255) tACCGCTCCCGGAGATATTAATGCGGGGGAATGGGGATTATCCGCCCTTCCCGGACGTGAGCACGAAGcac < 1:1400621/71‑1 (MQ=255) tACCGCTCCCGGAGATATTAATGCGGGGGAATGGGGATTATCCGCCCTTCCCGGACGTGAGCACGAAGcac < 1:537609/71‑1 (MQ=255) aCCGCTCCCGGAGATATTAATGCGGGGGAATGGGGATTATCCGCCCTTCCCGGACGTGAGCACGAAGcaca > 1:1116676/1‑71 (MQ=255) aCCGCTCCCGGAGATATTAATGCGGGGGAATGGGGATTATCCGCCCTTCCCGGACGTGAGCACGAAGcaca > 1:2265463/1‑71 (MQ=255) ccGCTCCCGGAGGTATTAATGCGGGGGAATGGGGATTATCCGCCCTTCCCGGACGTGAGCACGAAGCacac > 1:1114847/1‑71 (MQ=255) ccGCTCCCGGAGGTATTAATGCGGGGGAATGGGGATTATCCGCCCTTCCCGGACGTGAGCACGAAGCacac > 1:3790594/1‑71 (MQ=255) ccGCTCCCGGAGATATTAATGCGGGGGAATGGGGATTATCCGCCCTTCCCGGACGTGAGCACGAAGcaca > 1:4140442/1‑70 (MQ=255) ccGCTCCCGGAGATATTAATGCGGGGGAATGGGGATTATCCGCCCTTCCCGGACGTGAGCACGAAGcaca > 1:217774/1‑70 (MQ=255) ccGCTCCCGGAGATATTAATGCGGGGGAATGGGGATTATCCGCCCTTCCCGGACGTGAGCACGAAGCacac > 1:326595/1‑71 (MQ=255) cGCTCCCGGAGATATTAATGCGGGGGAATGGGGATTATCCGCCCTTCCCGGACGTGAGCACGAAGCACACg > 1:1988692/1‑71 (MQ=255) cGCTCCCGGAGATATTAATGCGGGGGAATGGGGATTATCCGCCCTTCCCGGACGTGAGCACGAAGCACACg > 1:3111596/1‑71 (MQ=255) cTCCCGGAGGTATTAATGCGGGGGAATGGGGATTATCCGCCCTTCCCGGACGTGAGCACGAAGCACACGCa < 1:3831183/71‑1 (MQ=255) cTCCCGGAGATATTAATGCGGGGGAATGGGGATTATCCGCCCTTCCCGGACGTGAGCACGAAGCacac > 1:1538280/1‑68 (MQ=255) cTCCCGGAGATATTAATGCGGGGGAATGGGGATTATCCGCCCTTCCCGGACGTGAGCACGAAGCACACg > 1:58409/1‑69 (MQ=255) cTCCCGGAGATATTAATGCGGGGGAATGGGGATTATCCGCCCTTCCCGGACGTGAGCACGAAGCACACg > 1:17023/1‑69 (MQ=255) cTCCCGGAGATATTAATGCGGGGGAATGGGGATTATCCGCCCTTCCCGGACGTGAGCACGAAGCACACg > 1:3326552/1‑69 (MQ=255) cGGAGATATTAATGCGGGGGAATGGGGATTATCCGCCCTTCCCGGACGTGAGCACGAAGCACACGCAGACa < 1:614809/71‑1 (MQ=255) cGGAGATATTAATGCGGGGGAATGGGGATTATCCGCCCTTCCCGGACGTGAGCACGAAGCACACGCAGACa < 1:1464251/71‑1 (MQ=255) | CCTGCAAATCCAAAAGCAACTGGAGCAAAACCATCTGACACTGGCGCTGTTCAATACCGCTCCCGGAGATATTAATGCGGGGGAATGGGGATTATCCGCCCTTCCCGGACGTGAGCACGAAGCACACGCAGACA > minE/1834026‑1834159 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |