| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,845,199 | G→T | 58.8% | P266T (CCG→ACG) | cysD ← | sulfate adenylyltransferase, subunit 2 |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,845,199 | 0 | G | T | 58.8% | 0.2 / 106.9 | 68 | P266T (CCG→ACG) | cysD | sulfate adenylyltransferase, subunit 2 |
| Reads supporting (aligned to +/- strand): ref base G (13/15); new base T (29/11); total (42/26) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.27e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.31e-01 | |||||||||||
CGCGGTCAATCACGCGGCCCTGACGTTCACTGGTGGTGGAAACCAGCATCTCTTCGATGATTTCCGGCAGTGTTTGTGCATTTGACTCCACCGCACCGGTCAGCGGCCAGCAGCCCAGCGTACGGAAACGCAC > minE/1845133‑1845265 | cgcgGTCAATCACGCGGCCCTGACGTTCACTGGTGGTGGAAACCAGCATCTCTTCGATGATTTCCGGCAgt > 1:3995339/1‑71 (MQ=255)cgcgGTCAATCACGCGGCCCTGACGTTCACTGGTGGTGGAAACCAGCATCTCTTCGATGATTTCCGGCAgt > 1:1736184/1‑71 (MQ=255) aaTCACGCGGCCCTGACGTTCACTGGTGGTGGAAACCAGCATCTCTTCGATGATTTCCGTCAGTGTTtgtg < 1:3993707/71‑1 (MQ=255) tCACGCGGCCCTGACGTTCACTGGTGGTGGAAACCAGCATCTCTTCGATGATTTCCGTCAGTGTTtg > 1:1865864/1‑67 (MQ=255) tCACGCGGCCCTGACGTTCACTGGTGGTGGAAACCAGCATCTCTTCGATGATTTCCGGCAGTGTTTGTGCa > 1:4055338/1‑71 (MQ=255) gcgGCCCTGACGTTCACTGGTGGTGGAAACCAGCATCTCTTCGATGATTTCCGGCAGTGTTTGTGCATTTg < 1:651525/71‑1 (MQ=255) gcgGCCCTGACGTTCACTGGTGGTGGAAACCAGCATCTCTTCGATGATTTCCGGCAGTGTTTGTGCATTTg < 1:537581/71‑1 (MQ=255) gcgGCCCTGACGTTCACTGGTGGTGGAAACCAGCATCTCTTCGATGATTTCCGGCAGTGTTTGTGCATTTg < 1:3843774/71‑1 (MQ=255) gACGTTCACTGGTGGTGGAAACCAGCATCTCTTCGATGATTTCCGTCAGTGTTTGTGCATTTGACTCcacc > 1:1509455/1‑71 (MQ=255) gACGTTCACTGGTGGTGGAAACCAGCATCTCTTCGATGATTTCCGTCAGTGTTTGTGCATTTGACTCcacc > 1:1608494/1‑71 (MQ=255) gACGTTCACTGGTGGTGGAAACCAGCATCTCTTCGATGATTTCCGTCAGTGTTTGTGCATTTGACTCcacc > 1:3611259/1‑71 (MQ=255) gACGTTCACTGGTGGTGGAAACCAGCATCTCTTCGATGATTTCCGTCAGTGTTTGTGCATTTGACTCcac > 1:1567048/1‑70 (MQ=255) gACGTTCACTGGTGGTGGAAACCAGCATCTCTTCGATGATTTCCGTCAGTGTTTGTGCATTTGACTCcac > 1:3978301/1‑70 (MQ=255) gACGTTCACTGGTGGTGGAAACCAGCATCTCTTCGATGATTTCCGTCAGTGTTTGTGCATTTGACTCcac > 1:2790119/1‑70 (MQ=255) gACGTTCACTGGTGGTGGAAACCAGCATCTCTTCGATGATTTCCGTCAGTGTTTGTGCATTTGACTCca > 1:3341279/1‑69 (MQ=255) gACGTTCACTGGTGGTGGAAACCAGCATCTCTTCGATGATTTCCGTCAGTGTTTGTGCATTTGACTCca > 1:1177856/1‑69 (MQ=255) gACGTTCACTGGTGGTGGAAACCAGCATCTCTTCGATGATTTCCGGCAGTGTTTGTGCATTTGACTCcacc > 1:848908/1‑71 (MQ=255) gACGTTCACTGGTGGTGGAAACCAGCATCTCTTCGATGATTTCCGGCAGTGTTTGTGCATTTGACTCcac > 1:1732141/1‑70 (MQ=255) aCGTTCACTGGTGGTGGAAACCAGCATCTCTTCGATGATTTCCGTCAGTGTTTGTGCATTTGACTCcaccg > 1:3531633/1‑71 (MQ=255) cGTTCACTGGTGGTGGAAACCAGCATCTCTTCGATGATTTCCGGCAGTGTTTGTGCATTTGACTCcaccgc < 1:3953593/71‑1 (MQ=255) ttCACTGGTGGTGGAAACCAGCATCTCTTCGATGATTTCCGGCAgtgt < 1:2954971/48‑1 (MQ=255) ttCACTGGTGGTGGAAACCAGCATCTCTTCGATGATTTCCGGCAgtgt < 1:3424284/48‑1 (MQ=255) tCACTGGTGGTGGAAACCAGCATCTCTTCGATGATTTCCGTCAGTGTTTGTGCATTTGACTCcaccgcacc > 1:1621804/1‑71 (MQ=255) cACTGGTGGTGGAAACCAGCATCTCTTCGATGATTTCCGGCAGTGTTTGTGCATTTGACTCcaccgcacc < 1:2412911/70‑1 (MQ=255) tggtggtggAAACCAGCATCTCTTCGATGATTTCCGTCAGTGTTTGTGCAttt < 1:2952906/53‑1 (MQ=255) tggtggtggAAACCAGCATCTCTTCGATGATTTCCGTCAGTGTTTGTGCAttt < 1:2249635/53‑1 (MQ=255) tggtggtggAAACCAGCATCTCTTCGATGATTTCCGTCAGTGTTTGTGCAttt < 1:3479560/53‑1 (MQ=255) tggtggtggAAACCAGCATCTCTTCGATGATTTCCGTCAGTGTTTGTGCAttt < 1:2607283/53‑1 (MQ=255) tggtggtggAAACCAGCATCTCTTCGATGATTTCCGTCAGTGTTTGTGCAttt < 1:695844/53‑1 (MQ=255) tggtggtggAAACCAGCATCTCTTCGATGATTTCCGTCAGTGTTTGTGCATTTGACTCCACCGCACCGGt > 1:3778542/1‑70 (MQ=255) tggtggtggAAACCAGCATCTCTTCGATGATTTCCGGCAGTGTTTGTGCAttt < 1:1123/53‑1 (MQ=255) gtggtggAAACCAGCATCTCTTCGATGATTTCCGGCAGTGTTTGTGCATTTGACTCCACCGCACCGGTCAg > 1:2354294/1‑71 (MQ=255) gtggtggAAACCAGCATCTCTTCGATGATTTCCGGCAGTGTTTGTGCATTTGACTCCACCGCACCGGTCAg > 1:2666561/1‑71 (MQ=255) gtggtggAAACCAGCATCTCTTCGATGATTTCCGGCAGTGTTTGTGCATTTGACTCCACCGCACCGGTCAg > 1:1783013/1‑71 (MQ=255) gtggAAACCAGCATCTCTTCGATGATTTCCGTCAGTGTTTGTGCATTTGACTCCACCGCACCGGTCAGCgg > 1:567843/1‑71 (MQ=255) gtggAAACCAGCATCTCTTCGATGATTTCCGGCAGTGTTTGTGCATTTGACTCCACCGCACCGGTCAGCgg > 1:2268850/1‑71 (MQ=255) tggAAACCAGCATCTCTTCGATGATTTCCGTCAGTGTTTGTGCATTTGACTCCACCGCACCGGTCAGCGGc < 1:3006529/71‑1 (MQ=255) tggAAACCAGCATCTCTTCGATGATTTCCGGCAGTGTTTGTGCATTTGACTCCACCGCACCGGTCAGCGGc < 1:206057/71‑1 (MQ=255) tggAAACCAGCATCTCTTCGATGATTTCCGGCAGTGTTTGTGCATTTGACTCCACCGCACCGGTCAGCGGc < 1:2670776/71‑1 (MQ=255) ggAAACCAGCATCTCTTCGATGATTTCCGTCAGTGTTTGTGCa < 1:207666/43‑1 (MQ=255) ggAAACCAGCATCTCTTCGATGATTTCCGGCAGTGTTTGTGCATTTGACTCCACCGCACCGGTCAGCGGc > 1:1423812/1‑70 (MQ=255) ggAAACCAGCATCTCTTCGATGATTTCCGGCAGTGTTTGTGCATTTGACTCCACCGCACCGGTCAGCGGc > 1:942381/1‑70 (MQ=255) aCCAGCATCTCTTCGATGATTTCCGTCAGTGTTTGTGCATTTGACTCCACCGCACCGGTCAGCGGCcag > 1:1456385/1‑69 (MQ=255) aCCAGCATCTCTTCGATGATTTCCGTCAGTGTTTGTGCATTTGACTCCACCGCACCGGTCAGCGGCcag > 1:1252290/1‑69 (MQ=255) aGCATCTCTTCGATGATTTCCGTCAGTGTTTGTGCATTTGACTCCACCGCACCGGTCAGCGGCcagcaggc < 1:2180930/71‑3 (MQ=255) aGCATCTCTTCGATGATTTCCGTCAGTGTTTGTGCATTTGACTCCACCGCACCGGTCAGCGGCCAGCAGcc < 1:3532325/71‑1 (MQ=255) aTCTCTTCGATGATTTCCGTCAGTGTTTGTGCATTTGACTCCACCGCACCGGTCAGCGGCCAGCAGCCCAg > 1:988109/1‑71 (MQ=255) aTCTCTTCGATGATTTCCGTCAGTGTTTGTGCATTTGACTCCACCGCACCGGTCAGCGGCCAGCAGCCCAg > 1:2159113/1‑71 (MQ=255) aTCTCTTCGATGATTTCCGTCAGTGTTTGTACATTTGACTCCACCGCACCGGTCAGCGGCCAGCAGCCCAg > 1:4118037/1‑71 (MQ=255) tctcTTCGATGATTTCCGTCTGTGTTTGTGCATTTGa > 1:3315762/1‑37 (MQ=37) tctcTTCGATGATTTCCGTCAGTGTTTGTGCATTTGa > 1:2599210/1‑37 (MQ=255) tctcTTCGATGATTTCCGTCAGTGTTTGTGCATTTGa > 1:3226304/1‑37 (MQ=255) tctcTTCGATGATTTCCGTCAGTGTTTGTGCATTTGa > 1:1650191/1‑37 (MQ=255) ctTCGATGATTTCCGGCAGTGTTTGTGCATTTGACTCcaccg > 1:3150209/1‑42 (MQ=255) ttCGATGATTTCCGTCAGTGTTTGTGCATTTGACTCCACCGCACCGGTAAGCGGCCAGGAGCCCAGCg < 1:2257988/68‑1 (MQ=255) ttCGATGATTTCCGGCAGTGTTTGTGCATTTGACTCCACCGCACCGGTCAGCGGCCAGCAGCCCAGCGt < 1:3558351/69‑1 (MQ=255) ttCGATGATTTCCGGCAGTGTTTGTGCATTTGACTCCACCGCACCGGTCAGCGGCCAGCAGCCCAGCGt < 1:111917/69‑1 (MQ=255) ttCGATGATTTCCGGCAGTGTTTGTGCATTTGACTCCACCGCACCGGTCAGCGGCCAGCAGCCCAGCGt < 1:1999958/69‑1 (MQ=255) ttCGATGATTTCCGGCAGTGTTTGTGCATTTGACTCCACCGCACCGGTCAGCGGCCAGCAGCCCAGCGt < 1:553640/69‑1 (MQ=255) ttCGATGATTTCCGGCAGTGTTTGTGCATTTGACTCCACCGCACCGGTCAGAGGCCAGCAGCCCAGCGt < 1:1524598/69‑1 (MQ=255) cGATGATTTCCGTCAGTGTTTGTGCATTTGACTCCACCGCACCGGTCAGCGGCCAGCAGCCCAg > 1:1163989/1‑64 (MQ=255) cGATGATTTCCGTCAGTGTTTGTGCATTTGACTCCACCGCACCGGTCAGCGGCCAGCAGCCCAGCGTACg > 1:2748069/1‑70 (MQ=255) cGATGATTTCCGTCAGTGTTTGTGCATTTGACTCCACCGCACCGGTCAGCGGCCAGCAGCCCAGCGTACg > 1:3408517/1‑70 (MQ=255) cGATGATTTCCGGCAGTGTTTGTGCATTTGACTCCACCGCACCGGTCAGCGGCca > 1:1161504/1‑55 (MQ=255) tCCGTCAGTGTTTGTGCATTTGACTCCACCGCACCGGTCAGCGGCcagca > 1:1033795/1‑50 (MQ=255) tCCGTCAGTGTTTGTGCATTTGACTCCACCGCACCGGTCAGCGGCcagca > 1:2000910/1‑50 (MQ=255) tCCGTCAGTGTTTGTGCATTTGACTCCACCGCACCGGTCAGCGGCcagca > 1:2402332/1‑50 (MQ=255) ccGTCAGTGTTTGTGCATTTGACTCCACCGCACCGGTCAGCGGCCAGCAGCCCAGCGTACGGAAACGCAc > 1:1871381/1‑70 (MQ=255) | CGCGGTCAATCACGCGGCCCTGACGTTCACTGGTGGTGGAAACCAGCATCTCTTCGATGATTTCCGGCAGTGTTTGTGCATTTGACTCCACCGCACCGGTCAGCGGCCAGCAGCCCAGCGTACGGAAACGCAC > minE/1845133‑1845265 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |