| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 247,251 | A→G | 8.3% | K298K (AAA→AAG) | phoR → | sensory histidine kinase in two‑component regulatory system with PhoB |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 247,251 | 0 | A | G | 8.3% | 159.0 / 7.1 | 60 | K298K (AAA→AAG) | phoR | sensory histidine kinase in two‑component regulatory system with PhoB |
| Reads supporting (aligned to +/- strand): ref base A (27/28); new base G (3/2); total (30/30) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.97e-01 | |||||||||||
TGAAAAGGTTGATGTGCCGATGATGCTGCGCGTTGTTGAGCGCGAGGCTCAGACTCTGAGTCAGAAAAAACAGACATTTACCTTTGAGATAGATAACGGCCTCAAGGTGTCTGGCAACGAAGATCAGCTACGCAGTG > minE/247182‑247318 | tGAAAAGGTTGATGTGCCGATGATGCTGCGCGTTGTTGAGCGCGAGGCTCAGACTCTGAGTCAGaaaaaa > 1:2487335/1‑70 (MQ=255) aaGGTTGATGTGCCGATGATGCTGCGCGTTGTTGAGCGCGAGGCTCAGACTCTGAGTCAGAAAAAACAGAc < 1:1306295/71‑1 (MQ=255) tGATGTGCCGATGATGCTGCGCGTTGTTGAGCGCGAGGCTCAGACTCTGAGTCAGAAAAAGCAGACATTTa > 1:2646430/1‑71 (MQ=255) tGATGTGCCGATGATGCTGCGCGTTGTTGAGCGCGAGGCTCAGACTCTGAGTCAGAAAAAGCAGACATTTa > 1:874740/1‑71 (MQ=255) tGATGTGCCGATGATGCTGCGCGTTGTTGAGCGCGAGGCTCAGACTCTGAGTCAGAAAAAGCAGACATTTa > 1:3906309/1‑71 (MQ=255) tgCCGATGATGCTGCGCGTTGTTGAGCGCGAGGCTCAGACTCTGAGTCAGAAAAAACAGACATTTACCttt > 1:2036274/1‑71 (MQ=255) gatgCTGCGCGTTGTTGAGCGCGAGGCTCAGACTCTGAGTCAGAAAAAACAGACATTTACCTTTgaga > 1:1159806/1‑68 (MQ=255) gatgCTGCGCGTTGTTGAGCGCGAGGCTCAGACTCTGAGTCAGAAAAAACAGACATTTACCTTTgaga > 1:175076/1‑68 (MQ=255) tgctgcGCGTTGTTGAGCGCTAGGCTCAGACTCTGAGTCAGAAAAAACAGACATTTACCTTTGagatagat < 1:2566518/71‑1 (MQ=255) tgctgcGCGTTGTTGAGCGCGAGGCTCAGACTCTGAGTCAGAAAAAACAGACATTTACCTTTGagatagat < 1:969077/71‑1 (MQ=255) tgctgcGCGTTGTTGAGCGCGAGGCTCAGACTCTGAGTCAGAAAAAACAGACATTTACCTTTGagatagat < 1:3249163/71‑1 (MQ=255) tgctgcGCGTTGTTGAGCGCGAGGCTCAGACTCTGAGTCAGAAAAAACAGACATTTACCTTTGagatagat < 1:2302473/71‑1 (MQ=255) tgctgcGCGTTGTTGAGCGCGAGGCTCAGACTCTGAGTCAGAAAAAACAGACATTTACCTTTGagatagat < 1:188984/71‑1 (MQ=255) tgctgcGCGTTGTTGAGCGCGAGGCTCAGACTCTGAGTCAGAAAAAACAGACATTTACCTTTGagatagat < 1:4132240/71‑1 (MQ=255) gctgcGCGTTGTTGAGCGCGAGGCTCAGACTCTGAGTCAGAAGAAACAGACATTTa > 1:916852/1‑56 (MQ=255) gctgcGCGTTGTTGAGCGCGAGGCTCAGACTCTGAGTCAGAAAAAACAGACATTTa > 1:1717463/1‑56 (MQ=255) gctgcGCGTTGTTGAGCGCGAGGCTCAGACTCTGAGTCAGAAAAAACAGACATTTa > 1:3562333/1‑56 (MQ=255) gctgcGCGTTGTTGAGCGCGAGGCTCAGACTCTGAGTCAGAAAAAACAGACATTTa > 1:3714705/1‑56 (MQ=255) gctgcGCGTTGTTGAGCGCGAGGCTCAGACTCTGAGTCAGAAAAAACAGACATTTa > 1:2370018/1‑56 (MQ=255) gctgcGCGTTGTTGAGCGCGAGGCTCAGACTCTGAGTCAGAAAAAACAGACATTTACCTTTGagatagat < 1:4059792/70‑1 (MQ=255) cgcgTTGTTGAGCGCGAGGCTCAGACTCTGAGTCAGAAAAAACAGACATTTACCTTTGAGATAGATAACgg > 1:2372788/1‑71 (MQ=255) tgttgAGCGCGAGGCTCAGACTCTGAGTCAGAAAAAACAGACATTTACCTTTGAGATAGATAACGGCCTc > 1:413026/1‑70 (MQ=255) gttgAGCGCGAGGCTCAGACTCTGAGTCAGAAAAAACAGACATTTACCTTTGAGATAGATAACGGCCTc > 1:2379067/1‑69 (MQ=255) gttgAGCGCGAGGCTCAGACTCTGAGTCAGAAAAAACAGACATTTACCTTTGAGATAGATAACGGCCTc > 1:1315369/1‑69 (MQ=255) gttgAGCGCGAGGCTCAGACTCTGAGTCAGAAAAAACAGACATTTACCTTTGAGATAGATAACGGCCTc > 1:3845404/1‑69 (MQ=255) gttgAGCGCGAGGCTCAGACTCTGAGTCAGAAAAAACAGACATTTACCTTTGAGATAGATAACGGCCTc > 1:242869/1‑69 (MQ=255) gttgAGCGCGAGGCTCAGACTCTGAGTCAGAAAAAACAGACATTTACCTTTGAGATAGATAACGGCCTc > 1:604630/1‑69 (MQ=255) gttgAGCGCGAGGCTCAGACTCTGAGTCAGAAAAAACAGACATTTACCTTTGAGATAGATAACGGCCTc > 1:539600/1‑69 (MQ=255) gttgAGCGCGAGGCTCAGACTCTGAGTCAGAAAAAACAGACATTTACCTTTGAGATAGATAACGGCCTc > 1:4110309/1‑69 (MQ=255) gttgAGCGCGAGGCTCAGACTCTGAGTCAGAAAAAACAGACATTTACCTTTGAGATAGATAACGGCCTCa < 1:922317/70‑1 (MQ=255) ttgAGCGCGAGGCTCAGACTCTGAGTCAGAAAAAACAGACATTTACCTTTGAGATAGATAACGGCCTCaa < 1:486371/70‑1 (MQ=255) ttgAGCGCGAGGCTCAGACTCTGAGTCAGAAAAAACAGACATTTACCTTTGAGATAGATAACGGCCTCaa < 1:3148149/70‑1 (MQ=255) cgAGGCTCAGACTCTGAGTCAGAAAAAACAGACATTTACCTTTGAGATAGATAACGGCCTCAAGGTGTCTg < 1:1884124/71‑1 (MQ=255) cAGACTCTGAGTCAGAAAAAACAGACATTTACCTTTGAGATAGATAACgc > 1:1124341/1‑49 (MQ=255) cAGACTCTGAGTCAGAAAAAACAGACATTTACCTTTGAGATAGATAACGGCCTCAAGgt > 1:4021464/1‑59 (MQ=255) cAGACTCTGAGTCAGAAAAAACAGACATTTACCTTTGAGATAGATAACGGCCTCAAGgt > 1:3440168/1‑59 (MQ=255) cAGACTCTGAGTCAGAAAAAACAGACATTTACCTTTGAGATAGATAACGGCCTCAAGgt > 1:3649081/1‑59 (MQ=255) cAGACTCTGAGTCAGAAAAAACAGACATTTACCTTTGAGATAGATAACGGCCTCAAGGTGTCTGGc < 1:2992110/66‑1 (MQ=255) cAGACTCTGAGTCAGAAAAAACAGACATTTACCTTTGAGATAGATAACGGCCTCAAGGTGTCTGGc < 1:1121687/66‑1 (MQ=255) cAGACTCTGAGTCAGAAAAAACAGACATTTACCTTTGAGATAGATAACGGCCTCAAGGTGTCTGGc < 1:1001642/66‑1 (MQ=255) cAGACTCTGAGTCAGAAAAAACAGACATTTACCTTTGAGATAGATAACGGCCTCAAGGTGTCTGGCAACg > 1:3979656/1‑70 (MQ=255) cAGACTCTGAGTCAGAAAAAACAGACATTTACCTTTGAGATAGATAACGGCCTCAAGGTGTCTGGCAACg > 1:1338295/1‑70 (MQ=255) aGACTCTGATTCAGAAAAAACAGACATTTACCTTTGAGATAGATAACGGCCTCAAGGTGTCTGGCAACg < 1:3394844/69‑1 (MQ=255) aGACTCTGAGTCAGAAAAAACAGACATTTACCTTTGAGATAGATAACGGCCTCAAGGTGTCTGGCAACg < 1:1117152/69‑1 (MQ=255) ctctGAGTCAGAAAAAACAGACATTTACCTTTgaga > 1:917622/1‑36 (MQ=255) ctctGAGTCAGAAAAAACAGACATTTACCTTTgaga > 1:3907713/1‑36 (MQ=255) ctctGAGTCAGAAAAAACAGACATTTACCTTTGAGATAGATAACGGCCTCAAGGTGTCTGGCAACGAAGAt < 1:3650140/71‑1 (MQ=255) ctctGAGTCAGAAAAAACAGACATTTACCTTTGAGATAGATAACGGCCTCAAGGTGTCTGGCAACGAAGAt < 1:1815639/71‑1 (MQ=255) ctctGAGTCAGAAAAAACAGACATTTACCTTTGAGATAGATAACGGCCTCAAGGTGTCTGGCAACGAAGAt < 1:241572/71‑1 (MQ=255) ctctGAGTCAGAAAAAACAGACATTTACCTTTGAGATAGATAACGGCCTCAAGGTGTCTGGCAACGAAGAt < 1:2655247/71‑1 (MQ=255) ctctGAGTCAGAAAAAACAGACATTTACCTTTGAGATAGATAACGGCCTCAAGGTGTCTGGCAACGAAGAt < 1:2109700/71‑1 (MQ=255) tctGAGTCAGAAAAAACAGACATTTACCTTTGAGATAGATAACGGCCTCAAGGTGTCTGTCAACGAAg < 1:260852/68‑1 (MQ=255) tctGAGTCAGAAAAAACAGACATTTACCTTTGAGATAGATAACGGCCTCAAGGTGTCTGGCAACGAAg < 1:3379399/68‑1 (MQ=255) tctGAGTCAGAAAAAACAGACATTTACCTTTGAGATAGATAACGGCCTCAAGGTGTCTGGCAACGAAg < 1:1156307/68‑1 (MQ=255) tctGAGTCAGAAAAAACAGACATTTACCTTTGAGATAGATAACGGCCTCAAGGTGTCTGGCAACGAAg < 1:2512006/68‑1 (MQ=255) tCAGAAAAAGCAGACATTTACCTTTGAGATAGATAACGGCCTCAAGGTGTCTGGCAACGAAGATCAGCTAc < 1:1563336/71‑1 (MQ=255) tCAGAAAAAGCAGACATTTACCTTTGAGATAGATAACGGCCTCAAGGTGTCTGGCAACGAAGATCAGCTAc < 1:2629461/71‑1 (MQ=255) tCAGAAAAAACAGACATTTACCTTTGAGATAGATAACGGCCTCAAGGTGTCTGGCAACGAAGa > 1:350590/1‑63 (MQ=255) aGAAAAAACAGACATTTACCTTTGAGATAGATAACGGCCTCAAGGTGTc > 1:608824/1‑49 (MQ=255) gAAAAAACAGACATTTACCTTTGAGATAGATAACGGCCTCAAGGTGTCTGGCAACGAAGATCAGCTACGCa < 1:2468961/71‑1 (MQ=255) gAAAAAACAGACATTTACCTTTGAGATAGATAACGGCCTCAAGGTGTCTGGCAACGAAGATCAGCTACGCa < 1:846426/71‑1 (MQ=255) aaaaCAGACATTTGCCTTTGAGATAGATAACGGCCTCAAGGTGTCTGGCAACGAAGATCAGCTACGCAGt > 1:4201026/1‑70 (MQ=255) aaaaCAGACATTTACCTTTGAGATAGATAACGGCCTCAAGGTGTTTGGCAACGAAGa < 1:3327024/57‑1 (MQ=255) aaaaCAGACATTTACCTTTGAGATAGATAACGGCCTCAAGGTGTCTGGCAACGAAGa < 1:1926851/57‑1 (MQ=255) aaaaCAGACATTTACCTTTGAGATAGATAACGGCCTCAAGGTGTCTGGCAACGAAGa < 1:146657/57‑1 (MQ=255) aaaaCAGACATTTACCTTTGAGATAGATAACGGCCTCAAGGTGTCTGGCAACGAAGa < 1:3716046/57‑1 (MQ=255) aaaaCAGACATTTACCTTTGAGATAGATAACGGCCTCAAGGTGTCTGGCAACGAAGa < 1:919715/57‑1 (MQ=255) aaaaCAGACATTTACCTTTGAGATAGATAACGGCCTCAAGGTGTCTGGCAACGAAGATCAGCTACGCAGTg > 1:3862970/1‑71 (MQ=255) aaaaCAGACATTTACCTTTGAGATAGATAACGGCCTCAAGGTGTCTGGCAACGAAGATCAGCTACGCAGTg > 1:3015755/1‑71 (MQ=255) aCAGACATTTACCTTTGAGATAGATAACGGCCTCAAGGTGTCTGGCAACGAAGATCAg > 1:4081446/1‑58 (MQ=255) | TGAAAAGGTTGATGTGCCGATGATGCTGCGCGTTGTTGAGCGCGAGGCTCAGACTCTGAGTCAGAAAAAACAGACATTTACCTTTGAGATAGATAACGGCCTCAAGGTGTCTGGCAACGAAGATCAGCTACGCAGTG > minE/247182‑247318 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |