| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,874,329 | A→G | 37.5% | I191T (ATT→ACT) | ygcF ← | conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,874,329 | 0 | A | G | 37.5% | 108.7 / 145.1 | 128 | I191T (ATT→ACT) | ygcF | conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base A (41/39); new base G (20/28); total (61/67) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.61e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 135 total aligned reads displayed.
CATCGACAAACGCCAATTACGCGCAATGCAGGTTTCAATGCACAAACGTGTGGCATCATCCTTTTGGCTAATCGGCTGCAGTGCAATGACTCGCGGTTTATCATCGGTCAGCGTTGCCAGTAGTTCATCCAGTGCTTCAAT > minE/1874259‑1874399 | cATCGACAAACGCCAATTACGCGCAATGCAGGTTTCAATGCACAAACGTGTGGCATCATCCTTTTGGCTaa < 1:2487214/71‑1 (MQ=255)cATCGACAAACGCCAATTACGCGCAATGCAGGTTTCAATGCACAAACGTGTGGCATCATCCTTTTGGCTaa < 1:152509/71‑1 (MQ=255) cGACAAACGCCAATTACGCGCAATGCAGGTTTCAATGCACAAACGTGTGGCATCATCCTTTTGGCTAATCg > 1:4133389/1‑71 (MQ=255) cGACAAACGCCAATTACGCGCAATGCAGGTTTCAATGCACAAACGTGTGGCATCATCCTTTTGGCTAATCg > 1:1339678/1‑71 (MQ=255) cGACAAACGCCAATTACGCGCAATGCAGGTTTCAATGCACAAACGTGTGGCATCATCCTTTTGGCTAATCg > 1:1663316/1‑71 (MQ=255) cGACAAACGCCAATTACGCGCAATGCAGGTTTCAATGCACAAACGTGTGGCATCATCCTTTTGGCTAATCg > 1:2862599/1‑71 (MQ=255) cGACAAACGCCAATTACGCACAATGCAGGTTTCAATGCACAAACGTGTGGCATCATCCTTTTGGCTAATCg > 1:3227708/1‑71 (MQ=255) gACAAACGCCAATTACGCGCAATGCAGGTTTCAATGCACAAACGTGTGGCATCATCCTTTTGGCTAGTCg > 1:2682664/1‑70 (MQ=255) gACAAACGCCAATTACGCGCAATGCAGGTTTCAATGCACAAACGTGTGGCATCATCCTTTTGGCTAATCgg > 1:2859655/1‑71 (MQ=255) aaaCGCCAATTACGCGCAATGCAGGTTTCAATGCACAAACGTGTGGCATCATCCTTTTGGCTAGTCGGCTg < 1:3751137/71‑1 (MQ=255) aaaCGCCAATTACGCGCAATGCAGGTTTCAATGCACAAACGTGTGGCATCATCCTTTTGGCTAATCGGCTg < 1:3063180/71‑1 (MQ=255) cGCCAATTACGCGCAATGCAGGTTTCAATGCACAAACGTGTGGCATCATCCTTTTGGCTAGTCGGCTg > 1:452303/1‑68 (MQ=255) gCCAATTACGCGCAATGCAGGTTTCAATGCACAAACGTGTGGCATCATCCTTTTGGCTAGTCGGCTGCAGt < 1:1031981/71‑1 (MQ=255) gCCAATTACGCGCAATGCAGGTTTCAATGCACAAACGTGTGGCATCATCCTTTTGGCTAGTCGGCTGCAGt < 1:117905/71‑1 (MQ=255) ttACGCGCAATGCAGGTTTCAATGCACAAACGTGTGGCATCATCCTTTTGGCTAATc < 1:1423260/57‑1 (MQ=255) ttACGCGCAATGCAGGTTTCAATGCACAAACGTGTGGCATCATCCTTTTGGCTAATc < 1:2817833/57‑1 (MQ=255) ttACGCGCAATGCAGGTTTCAATGCACAAACGTGTGGCATCATCCTTTTGGCTAATc < 1:2947907/57‑1 (MQ=255) ttACGCGCAATGCAGGTTTCAATGCACAAACGTGTGGCATCATCCTTTTGGCTAATCGGCTGCAGTGCAAt < 1:1507644/71‑1 (MQ=255) ttACGCGCAATGCAGGTTTCAATGCACAAACGTGTGGCATCATCCTTTTGGCTAATCGGCTGCAGTGCAAt < 1:2823113/71‑1 (MQ=255) ttACGCGCAATGCAGGTTTCAATGCACAAACGTGTGGCATCATCCTTTTGGCTAATCGGCTGCAGTGCAAt < 1:1392191/71‑1 (MQ=255) ttACGCGCAATGCAGGTTTCAATGCACAAACGTGTGGCATCATCCTTTTGGCTAATCGGCTGCAGTGCAAt < 1:1113753/71‑1 (MQ=255) tACGCGCAATGCAGGTTTCAATGCACAAACGTGTGGCATCATCCTTTTGGCTAGTCGGCTGCAGTGCAAt > 1:1854182/1‑70 (MQ=255) aCGCGCAATGCAGGTTTCAATGCACAAACGTGTGGCATCATCCTTTTGGCTAATCGGCTGCAGTGCAATGa > 1:2310221/1‑71 (MQ=255) cAATGCAGGTTTCAATGCACAAACGTGTGGCATCATCCTTTTGGCTAGTCGGCTGCAGTGCAATGACTcgc < 1:2835280/71‑1 (MQ=255) cAATGCAGGTTTCAATGCACAAACGTGTGGCATCATCCTTTTGGCTAGTCGGCTGCAGTGCAATGACTcgc < 1:3951989/71‑1 (MQ=255) tGCAGGTTTCAATGCACAAACGTGTGGCATCATCCTTTTGGCTAGTCGGCTg < 1:104336/52‑1 (MQ=255) tGCAGGTTTCAATGCACAAACGTGTGGCATCATCCTTTTGGCTAGTCGGCTg < 1:4212606/52‑1 (MQ=255) tGCAGGTTTCAATGCACAAACGTGTGGCATCATCCTTTTGGCTAGTCGGCTGCAGTGCAATGACTCGCGGt > 1:306649/1‑71 (MQ=255) gCAGGTTTCAATGCACAAACGTGTGGCATCATCCTTTTGGCTAATCGGCTGCAGTGCAATGACTCGCGGtt > 1:1363130/1‑71 (MQ=255) cAGGTTTCAATGCACAAACGTGTGGCATCATCCTTTTGGCTAATCGGCTGCAGTGCAATGACTCGCGGtt > 1:2791406/1‑70 (MQ=255) aaGGTTTCAATGCACAAACGTGTGGCATCATCCTTTTGGCTAATCGGCTGCAGTGCAATGACTCGCGGtt > 1:2231279/2‑70 (MQ=255) aGGTTTCAATGCACAAACGTGTGGCATCATCCTTTTGGCTAATCGGCTGCAGTGCAATGACTCGCGGTTTa < 1:158802/71‑1 (MQ=255) gTTTCAATGCACAAACGTGTGGCATCATCCTTTTGGCTAGTCGTCTGc < 1:4103864/48‑1 (MQ=255) gTTTCAATGCACAAACGTGTGGCATCATCCTTTTGGCTAGTCGGCTGc < 1:2703830/48‑1 (MQ=255) gTTTCAATGCACAAACGTGTGGCATCATCCTTTTGGCTAGTCGGCTGc < 1:777997/48‑1 (MQ=255) gTTTCAATGCACAAACGTGTGGCATCATCCTTTTGGCTAGTCGGCTGc < 1:2059170/48‑1 (MQ=255) gTTTCAATGCACAAACGTGTGGCATCATCCTTTTGGCTAGTCGGCTGCAGTGc < 1:627334/53‑1 (MQ=255) gTTTCAATGCACAAACGTGTGGCATCATCCTTTTGGCTAGTCGGCTGCAGTGc < 1:1317096/53‑1 (MQ=255) gTTTCAATGCACAAACGTGTGGCATCATCCTTTTGGCTAGTCGGCTGCAGTGc < 1:3763788/53‑1 (MQ=255) gTTTCAATGCACAAACGTGTGGCATCATCCTTTTGGCTAGTCGGCTGCAGTGc < 1:1221580/53‑1 (MQ=255) gTTTCAATGCACAAACGTGTGGCATCATCCTTTTGGCTAATCGGCTGCAGTGCAATGACTCGCGGTTTat > 1:2324323/1‑70 (MQ=255) gTTTCAATGCACAAACGTGTGGCATCATCCTTTTGGCTAATCGGCTGCAGTGCAATGACTCGCGGTTTat > 1:2902771/1‑70 (MQ=255) ttCAATGCACAAACGTGTGGCATCATCCTTTTGGCTAGTCGGCTGCAGTGCaa > 1:1516802/1‑53 (MQ=255) tCAATGCACAAACGTGTGGCATCATCCTTTTGGCTAGTCGGCTGCAGTGCAATGACTCGCGGTTTatcatc > 1:1656579/1‑71 (MQ=255) tCAATGCACAAACGTGTGGCATCATCCTTTTGGCTAGTCGGCTGCAGTGCAATGACTCGCGGTTTatcatc > 1:1176646/1‑71 (MQ=255) tCAATGCACAAACGTGTGGCATCATCCTTTTGGCTAATCGGCTGCAGTGCAATGACTCGCGGTTTa < 1:348051/66‑1 (MQ=255) cAATGCACAAACGTGTGGCATCATCCTTTTGGCTAATCGGCTGCAGTGCAATGACTCGCGGTTTATCATCg > 1:817458/1‑71 (MQ=255) cAATGCACAAACGTGTGGCATCATCCTTTTGGCTAATCGGCTGCAGTGAAATGACTCGCGGTTTATCATCg > 1:1766842/1‑71 (MQ=255) cacaAACGTGTGGCATCATCCTTTTGGCTAATCGGCTGCAGTGCAATGACTCGCGGTTTATCATCGGTCa < 1:3393693/70‑1 (MQ=255) cacaAACGTGTGGCATCATCCTTTTGGCTAATCGGCTGCAGTGCAATGACTCGCGGTTTATCATCGGTCa < 1:1228515/70‑1 (MQ=255) cacaAACGTGTGGCATCATCCTTTTGGCTAATCGGCTGCAGTGCAATGACTCGCGGTTTATCATCGGTCAg > 1:250733/1‑71 (MQ=255) cacaAACGTGTGGCATCATCCTTTTGGCTAATCGGCTGCAGTGCAATGACTCGCGGTTTATCATCGGTCAg > 1:1993338/1‑71 (MQ=255) cacaAACGTGTGGCATCATCCTTTTGGCTAATCGGCTGCAGTGCAATGACTCGCGGTTTATCATCGGTCAg > 1:4178050/1‑71 (MQ=255) acaAACGTGTGGCATCATCCTTTTGGCTAGTCGGCTGCAGTGCAATGACTCGCGGTTTATCATCGGTCAGc < 1:499104/71‑1 (MQ=255) acaAACGTGTGGCATCATCCTTTTGGCTAATCGGCTGCAGTGCAATGACTCGCGGTTTATCATCGGTCAGc < 1:347374/71‑1 (MQ=255) acaAACGTGTGGCATCATCCTTTTGGCTAATCGGCTGCAGTGCAATGACTCGCGGTTTATCATCGGTCAGc < 1:1584363/71‑1 (MQ=255) aaCGTGTGGCATCATCCTTTTGGCTAATCGGCTGCATTGCAATGACt < 1:859080/47‑1 (MQ=255) aaCGTGTGGCATCATCCTTTTGGCTAATCGGCTGCAGTGCAATGACt < 1:2899773/47‑1 (MQ=255) aaCGTGTGGCATCATCCTTTTGGCTAATCGGCTGCAGTGCAATGACt < 1:649950/47‑1 (MQ=255) aaCGTGTGGCATCATCCTTTTGGCTAATCGGCTGCAGTGCAATGACt < 1:29835/47‑1 (MQ=255) gtgtGGCAGCATCCTTTTGGCTAGTCGGGTGCAGt < 1:545055/35‑1 (MQ=25) tgGCATCATCCTTTTGGCTAATCGGCTGCAGTGCAATGACTCGCGGTTTATCATCGGTCAGCGTTGCCagt < 1:4115170/71‑1 (MQ=255) tgGCATCATCCTTTTGGCTAATCGGCTGCAGTGCAATGACTCGCGGTTTATCATCGGTCAGCGTTGCCagt < 1:1961646/71‑1 (MQ=255) tgGCATCATCCTTTTGGCTAATCGGCTGCAGTGCAATGACTCGCGGTTTATCATCGGTCAGCGTTGCCagt < 1:1480577/71‑1 (MQ=255) tgGCATCATCCTTTTGGCTAATCGGCTGCAGTGCAATGACTCGCGGTTTATCATCGGTCAGCGTTGCCagt < 1:2939795/71‑1 (MQ=255) tgGCATCATCCTTTTGGCTAATCGGCTGCAGTGCAATGACTCGCGGTTTATCATCGGTCAGCGTTGCCagt < 1:2768870/71‑1 (MQ=255) tgGCATCATCCTTTTGGCTAATCGGCTGCAGTGCAATGACTCGCGGTTTATCATCGGTCAGCGTTGCCagt < 1:3033939/71‑1 (MQ=255) gCATCATGCTTTTGGCTAGTCGGCTGCAGTGCAATGACTCGCGGTTTATCATCGGTCAGCGTTGc > 1:3137657/1‑65 (MQ=255) gCATCATCCTTTTGGCTAATCGGCTGCAGTGCAATGACTCGCGGTTTATCATCGGTCAGCGTTGCCagtag > 1:1924474/1‑71 (MQ=255) gCATCATCCTTTTGGCTAATCGGCTGCAGTGCAATGACTCGCGGTTTATCATCGGTCAGCGTTGCCagtag > 1:4234502/1‑71 (MQ=255) gatcCTTTTGGCTAATCGGCTGCAGTGCAATGACTCGCGGTTTATCATCGGTCa < 1:2001263/53‑1 (MQ=255) catcCTTTTGGCTAATCGGCTGCAGTGCAATGACTCGCGGTTTATCATCGGTCAGCGTTGCCAGTAGTTCa > 1:1767239/1‑71 (MQ=255) atcCTTTTGGCTAGTCGGCTGCAGTGCAATGACTCGCGGTTTATCATCGGTCAGCGTTGCCAGTAGTTCAt > 1:5952/1‑71 (MQ=255) atcCTTTTGGCTAGTCGGCTGCAGTGCAATGACTCGCGGTTTATCATCGGTCAGCGTTGCCAGTAGTTCAt > 1:2626114/1‑71 (MQ=255) atcCTTTTGGCTAGTCGGCTGCAGTGCAATGACTCGCGGTTTATCATCGGTCAGCGTTGCCAGTAGTTCAt > 1:3312219/1‑71 (MQ=255) atcCTTTTGGCTAGTCGGCTGCAGTGCAATGACTCGCGGTTTATCATCGGTCAGCGTTGCCAGTAGTTCAt > 1:1906246/1‑71 (MQ=255) tcCTTTTGGCTAATCGGCTGCAGTGCAATGACTCGCGGTTTATCATCGGTCAGCGTTGCCAGTAGTTCATc < 1:2912709/71‑1 (MQ=255) tcCTTTTCGCTAATCGGCTGCAGTGCAATGACTCGCGGTTTATCATCGGTCAg < 1:4240920/53‑1 (MQ=255) cTTTTGGCTAATCGGCTGCAGTGCAGTGACTCGCGGTTTATCATCGGTCAGCGTTGCCa > 1:2759690/1‑59 (MQ=255) cTTTTGGCTAATCGGCTGCAGTGCAATGACTCGCGGTTTATCATCGGTCAGCGTTGCCa > 1:3579433/1‑59 (MQ=255) cTTTTGGCTAATCGGCTGCAGTGCAATGACTCGCGGTTTATCATCGGTCAGCGTTGCCa > 1:481038/1‑59 (MQ=255) ttttGGCTAATCGGCTGCAGTGCAATGACTCGCGGTTTATCATCGGTCAGCGTTGcc < 1:2710178/57‑1 (MQ=255) ttGGCTAGTCGGCTGCAGTGCAATGACTCGCGGTTTATCATTGGTCAGCGTTGCCAGTAGTTCATCCAGt < 1:1168037/70‑1 (MQ=255) ttGGCTAGTCGGCTGCAGTGCAATGACTCGCGGTTTATCATCGGTCAGCGTTGCCAGTAGTTCATCCAGt < 1:376056/70‑1 (MQ=255) ttGGCTAGTCGGCTGCAGTGCAATGACTCGCGGTTTATCATCGGTCAGCGTTGCCAGTAGTTCATCCAGt < 1:1669542/70‑1 (MQ=255) ttGGCTAGTCGGCTGCAGTGCAATGACTCGCGGTTTATCATCGGTAAGCGTTGCCAGTAGTTCATCCAGt < 1:1988341/70‑1 (MQ=255) ttGGCTAATCGGCTGCAGTGCAATGACTCGCGGTTTATCATCGGTCAGCGTTGCCAGTAGTTCATCCAGTg > 1:2033887/1‑71 (MQ=255) gCTAATCGGCTGCAGTGCAATGACTCGCGGTTTATCATCGGTCAGCGTTGCCAGTAGTTCATCCAGTGCtt < 1:3919291/71‑1 (MQ=255) tAATCGGCTGCAGTGCAATGACTCGCGGTTTATCATCGGTCAGc < 1:935282/44‑1 (MQ=255) tAATCGGCTGCAGTGCAATGACTCGCGGTTTATCATCGGTCAGc < 1:418814/44‑1 (MQ=255) tAATCGGCTGCAGTGCAATGACTCGCGGTTTATCATCGGTCAGc < 1:2254817/44‑1 (MQ=255) tAATCGGCTGCAGTGCAATGACTCGCGGTTTATCATCGGTCAGCGTTGCCagtag > 1:3379610/1‑55 (MQ=255) tAATCGGCTGCAGTGCAATGACTCGCGGTTTATCATCGGTCAGCGTTGCCagtag > 1:1983806/1‑55 (MQ=255) tAATCGGCTGCAGTGCAATGACTCGCGGTTTATCATCGGTCAGCGTTGCCAGTAGTTCATCCAGTGCTTc > 1:3396477/1‑70 (MQ=255) tAATCGGCTGCAGTGCAATGACTCGCGGTTTATCATCGGTCAGCGTTGCCAGTAGTTCATCCAGTGCTTc > 1:3353297/1‑70 (MQ=255) tAATCGGCTGCAGTGCAATGACTCGCGGTTTATCATCGGTCAGCGTTGCCAGTAGTTCATCCAGTGCTTc > 1:1379914/1‑70 (MQ=255) aTCGGCTGCAGTGCAATGACTCGCGGTTTATCATCGGTCAGCGTTGCCAGTAGTTCATCCAGTGCTTCa < 1:917845/69‑1 (MQ=255) aTCGGCTGCAGTGCAATGACTCGCGGTTTATCATCGGTCAGCGTTGCCAGTAGTTCATCCAGTGCTTCAat < 1:3023129/71‑1 (MQ=255) aTCGGCTGCAGTGCAATGACTCGCGGTTTATCATCGGTCAGCGTTGCCAGTAGTTCATCCAGTGCTTCAat < 1:2950460/71‑1 (MQ=255) aTCGGCTGCAGTGCAATGACTCGCGGTTTATCATCGGTCAGCGTTGCCAGTAGTTCATCCAGTGCTTCAat < 1:2258845/71‑1 (MQ=255) | CATCGACAAACGCCAATTACGCGCAATGCAGGTTTCAATGCACAAACGTGTGGCATCATCCTTTTGGCTAATCGGCTGCAGTGCAATGACTCGCGGTTTATCATCGGTCAGCGTTGCCAGTAGTTCATCCAGTGCTTCAAT > minE/1874259‑1874399 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |