| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,883,198 | A→G | 50.4% | F429L (TTC→CTC) | rumA ← | 23S rRNA (uracil‑5)‑methyltransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,883,198 | 0 | A | G | 50.4% | 4.2 / 177.7 | 115 | F429L (TTC→CTC) | rumA | 23S rRNA (uracil‑5)‑methyltransferase |
| Reads supporting (aligned to +/- strand): ref base A (28/29); new base G (32/26); total (60/55) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.77e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.83e-01 | |||||||||||
Only 100 of 130 total aligned reads displayed.
AACCATCGTCCTCTCCTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAACTATTTAACGCGCGAGAAAAGTACCATCGATTCCAGATGTCCCGTGTGTGGGAACATATCCAGCATCGCCAGTCGCGCAATGGT > minE/1883128‑1883264 | aaCCATCGTCCTCTCCTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAACTATTTAACGCGCGAGaa < 1:1096725/71‑1 (MQ=255)aaCCATCGTCCTCTCCTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAACTATTTAACGCGCGAGaa < 1:2738239/71‑1 (MQ=255)aaCCATCGTCCTCTCCTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAACTATTTAACGCGCGAGaa < 1:2111209/71‑1 (MQ=255) aCCATCGTCCTCTCCTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAACTATTTAACGCGCGAGaaa < 1:1759678/71‑1 (MQ=255) aCCATCGTCCTCTCCTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAACTATTTAACGCGCGAGaaa < 1:2541283/71‑1 (MQ=255) ccATCGTCCTCTCCTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAACTATTTAACGCGCGAGaaa < 1:435983/70‑1 (MQ=255) cATCGTCCTCTCCTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAACTATTTAACGCGCGAGagaa < 1:287681/70‑1 (MQ=255) cATCGTCCTCTCCTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAACTATTTAACGCGCGAGAGAAg > 1:960709/1‑71 (MQ=255) tCGTCCTCTCCTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAACTATTTAACGCGCGAGAGAAGTa < 1:372776/71‑1 (MQ=255) cGTCCTCTCCTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAACTATTTAACGCGCGAGAAAAGTAc > 1:4102333/1‑71 (MQ=255) cGTCCTCTCCTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAACTATTTAACGCGCGAGAAAAGTAc > 1:3011788/1‑71 (MQ=255) gTCCTCTCCTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAACTATTTAACGCGCGAGAGAAGTAc > 1:2385400/1‑70 (MQ=255) gTCCTCTCCTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAACTATTTAACGCGCGAGAGAAGTAc > 1:1850570/1‑70 (MQ=255) tcCTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAACTATTTAACGCGCGAGAAAAGTACCATCGAt > 1:881587/1‑71 (MQ=255) tcCTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAACTATTTAACGCGCGAGAAAAGTACCATCGAt > 1:2071470/1‑71 (MQ=255) cTTTAGGGACTAGACCTGCCGAAATCGGCAAATCGCAACTATTTAACGCGCGAGAGAAGTACCATCGATTc > 1:2760422/1‑71 (MQ=255) cTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAACTATTTAACGCGCGAGAGAAGTACCa > 1:4206801/1‑64 (MQ=255) cTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAACTATTTAACGCGCGAGAGAAGTACCa > 1:1304308/1‑64 (MQ=255) cTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAACTATTTAACGCGCGAGAGAAGTACCa > 1:1228623/1‑64 (MQ=255) cTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAACTATTTAACGCGCGAGAGAAGTACCATCGAt < 1:1747893/69‑1 (MQ=255) cTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAACTATTTAACGCGCGAGAGAAGTACCATCGAt < 1:3379766/69‑1 (MQ=255) cTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAACTATTTAACGCGCGAGAGAAGTACCATCGAt < 1:1561432/69‑1 (MQ=255) cTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAACTATTTAACGCGCGAGAGAAGTACCATCGAt < 1:1466135/69‑1 (MQ=255) cTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAACTATTTAACGCGCGAGAGAAGTACCATCGAt < 1:3822309/69‑1 (MQ=255) cTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAACTATTTAACGCGCGAGAGAAGTACCATCGAt < 1:4035607/69‑1 (MQ=255) tttAGGGACCAGACCTGCCGAAATCGGCAAATCGCAACTATTTAACGCGCGAGAGAAGTACCATCGAt < 1:904737/68‑1 (MQ=255) tAGGGACCAGACCTGCCGAAATCGGCAAATCGCAACTATTTAACGGGCGAGAGAAGTAc > 1:215283/1‑59 (MQ=255) tAGGGACCAGACCTGCCGAAATCGGCAAATCGCAACTATTTAACGCGCGAGAGAAGTAc > 1:2365470/1‑59 (MQ=255) tAGGGACCAGACCTGCCGAAATCGGCAAATCGCAACTATTTAACGCGCGAGAGAAGTACCATCGATTc > 1:3020873/1‑68 (MQ=255) cAGACCTGCCGAAATCGGCAAATCGCAACTATTTAACGCGCGAGAGAAGTACCATCGATTCCAGa > 1:3586535/1‑65 (MQ=255) cAGACCTGCCGAAATCGGCAAATCGCAACTATTTAACGCGCGAGAGAAGTACCATCGATTCCAGa > 1:1953290/1‑65 (MQ=255) cAGACCTGCCGAAATCGGCAAATCGCAACTATTTAACGCGCGAGAGAAGTACCATCGATTCCAGa > 1:3745872/1‑65 (MQ=255) aGACCTGCCGAAATCGGCAAATCGCAACTATTTAACGCGCGAGAAAAGTAc > 1:1898781/1‑51 (MQ=255) aGACCTGCCGAAATCGGCAAATCGCAACTATTTAACGCGCGAGAAAAGTAc > 1:2234742/1‑51 (MQ=255) aGACCTGCCGAAATCGGCAAATCGCAACTATTTAACGCGCGAGAAAAGTACCATCGATTCCAGATGTCCCg < 1:2857786/71‑1 (MQ=255) aGACCTGCCGAAATCGGCAAATCGCAACTATTTAACGCGCGAGAAAAGTACCATCGATTCCAGATGTCCCg < 1:2926657/71‑1 (MQ=255) aGACCTGCCGAAATCGGCAAATCGCAACTATTTAACGCGCGAGAAAAGTACCATCGATTCCAGATGTCCCg < 1:1890419/71‑1 (MQ=255) aGACCTGCCGAAATCGGCAAATCGCAACTATTTAACGCGCGAGAAAAGTACCATCGATTCCAGATGTCCCg < 1:1943121/71‑1 (MQ=255) gACCTGCCGAAATCGGCAAATCGCAACTATTTAACGCGCGAGagaa > 1:3227766/1‑46 (MQ=255) gACCTGCCGAAATCGGCAAATCGCAACTATTTAACGCGCGAGagaa > 1:2759870/1‑46 (MQ=255) aCCTGCCGAAATTGGCAAATCGCAACTATTTAACGCGCGAGAAAAGTACCATCGATTCCAGATGTCCCgtg < 1:1245877/71‑1 (MQ=255) aCCTGCCGAAATCGGCAAATCGCAACTATTTAACGCGCGAGAAAAGTACCATCGATTCCAGATGTCCCgtg < 1:1467590/71‑1 (MQ=255) aCCTGCCGAAATCGGCAAATCGCAACTATTTAACGCGCGAGAAAAGTACCATCGATTCCAGATGTCCCgtg < 1:2063280/71‑1 (MQ=255) aCCTGCCGAAATCGGCAAATCGCAACTATTTAACGCGCGAGAAAAGTACCATCGATTCCAGATGTCCCgtg < 1:1729484/71‑1 (MQ=255) aCCTGCCGAAATCGGCAAATCGCAACTATTTAACGCGCGAGAAAAGTACCATCGATTCCAGATGTCCCgtg < 1:3147256/71‑1 (MQ=255) ccTGCCGAAATCGGCAAATCGCAACTATTTAACGCGCGAGAGAAGTACCATCGATTCCAGAt > 1:1444942/1‑62 (MQ=255) gCCGAAATCGGCAAATCGCAACTATTTAACGCGCGAGAAAAGTACCATCGATTCCAGATGTCCCGtgtgtg > 1:1046224/1‑71 (MQ=255) ccGAAATCGGCAAATCGCAACTATTTAACGCGCGAGAGAAGTACCATCGATTCCAGATGTCCCGtgtgtg < 1:2676433/70‑1 (MQ=255) ccGAAATCGGCAAATCGCAACTATTTAACGCGCGAGAGAAGTACCATCGATTCCAGATGTCCCGtgtgtg < 1:696391/70‑1 (MQ=255) ccGAAATCGGCAAATCGCAACTATTTAACGCGCGAGAGAAGTACCATCGATTCCAGATGTCCCGtgtgtg < 1:1935455/70‑1 (MQ=255) gAAATCGGCAAATCGCAACTATTTAACGCGCGAGAGAAGTACCATCGATTCCAGATGTCCCGTGTGTGGGa < 1:1507478/71‑1 (MQ=255) gAAATCGGCAAATAGCAACTATTTAACGCGCGAGAAAAGTACCATCGATTCCAGATGTCCCGTGTGTggg > 1:351598/1‑70 (MQ=255) aaaTCGGCAAATCGCAACTATTTAACGCGCGAGAGAAGt < 1:2511762/39‑1 (MQ=255) aaaTCGGCAAATCGCAACTATTTAACGCGCGAGAAAAGTACCATCGATTCCAGATGTCCCGTGTGTGGGaa < 1:3622815/71‑1 (MQ=255) aaaTCGGCAAATCGCAACTATTTAACGCGCGAGAAAAGTACCATCGATTCCAGATGTCCCGTGTGTGGGaa < 1:968648/71‑1 (MQ=255) aaaTCGGCAAATCGCAACTATTTAACGCGCGAGAAAAGTACCATCGATTCCAGATGTCCCGTGTGTGGGaa < 1:2946517/71‑1 (MQ=255) aaTCGGCAAATCGCAACTATTTAACGCGCGAGAGAAGTACCATCGATTCCAGATGTCCCGTGTGTGGGAAc < 1:3119784/71‑1 (MQ=255) aaTCGGCAAATCGCAACTATTTAACGCGCGAGAGAAGTACCATCGATTCCAGATGTCCCGTGTGTGGGAAc < 1:2441850/71‑1 (MQ=255) aTCGGCAAATCGCAACTATTTAACGCGCGAGAGAAGTAc > 1:1434089/1‑39 (MQ=255) cGGCAAATCGCAACTATTTAACGCGCGAGAGAAGTACCATCGATTCCAg < 1:3205699/49‑1 (MQ=255) cGGCAAATCGCAACTATTTAACGCGCGAGAGAAGTACCATCGATTCCAg < 1:27633/49‑1 (MQ=255) cGGCAAATCGCAACTATTTAACGCGCGAGAGAAGTACCATCGATTCCAGATGTCCCGTGTGTGGGAACat > 1:2874985/1‑70 (MQ=255) ggCAAATCGCAACTATTTAACGCGCGAGAGAAGTACCATCGATTCCAg < 1:4006520/48‑1 (MQ=255) gCAAATCGCAACTATTTAACGCGCGAGAAAAGTACCATCGATTCCAGATGTCCCGTGTGTGGGaa > 1:3239766/1‑65 (MQ=255) cAAATCGCAACTATTTAACGCGCGAGAAAAGTACCATCGATTCCAGATGTCCCGTGTGTGGGAACATATcc < 1:227766/71‑1 (MQ=255) cAAATCGCAACTATTTAACGCGCGAGAAAAGTACCATCGATTCCAGATGTCCCGTGTGTGGGAACATATcc < 1:348674/71‑1 (MQ=255) aaaTCGCAACTATTTAACGCGCGAGAAAAGTACCATCGATTCCAGATGTCCCGTGTGTGGGAACATATc > 1:3407659/1‑69 (MQ=255) aaTCGCAACTATTTAACGCGCGAGAGAAGTACCATCGATTCCAGATGTCCCGTGTGTGGGAACATATCCAg > 1:479698/1‑71 (MQ=255) aaTCGCAACTATTTAACGCGCGAGAAAAGTACCATCGATTCCa < 1:910428/43‑1 (MQ=255) aaTCGCAACTATTTAACGCGCGAGAAAAGTACCATCGATTCCa < 1:2048908/43‑1 (MQ=255) aTCGCAACTATTTAACGCGCGAGAAAAGTACCATCGATTCCAGATGTCCCGTGTGTGGGaa < 1:4004507/61‑1 (MQ=255) aTCGCAACTATTTAACGCGCGAGAAAAGTACCATCGATTCCAGATGTCCCGTGTGTGGGaa < 1:3345591/61‑1 (MQ=255) aTCGCAACTATTTAACGCGCGAGAAAAGTACCATCGATTCCAGATGTCCCGTGTGTGGGAACATATCCAg > 1:1660430/1‑70 (MQ=255) aTCGCAACTATTTAACGCGCGAGAAAAGTACCATCGATTCCAGATGTCCCGTGTGTGGGAACATATCCAg > 1:1138343/1‑70 (MQ=255) tCGCAACTATTTAACGCGCGAGAGAAGTACCATCGATTCCAGATGTCCCGTGTGTGGGAACATATCCAGCa > 1:2536194/1‑71 (MQ=255) cGCAACTATTTAACGCGCGAGAGAAGTACCATCGATTCCAGATGTCCCGTGTGTGGGAACATATCCAGCAt > 1:4100126/1‑71 (MQ=255) aaCTATTTAACGCGCGAGAGAAGTACCATCGATTCCAGATGTCCCGTGTGTGGGAACATATCCAGCATCGc < 1:3524598/71‑1 (MQ=255) aCTATTTAACGCGCGAGAAAAGTACCATCGATTCCAGATGTCCCGTGTGTGGGaa < 1:795321/55‑1 (MQ=255) cTATTTAACGCGCGAGAGAAGTACCATCGATTCCAGATGTCCCGTGTGTGGGaa < 1:2615068/54‑1 (MQ=255) cTATTTAACGCGCGAGAGAAGTACCATCGATTCCAGATGTCCCGTGTGTGGGAACATATCCAGCATCGc > 1:76360/1‑69 (MQ=255) cTATTTAACGCGCGAGAAAAGTACCATCGATTCCAGATGTCCCGTGTGTGGGAACATATCCa > 1:1294399/1‑62 (MQ=255) cTATTTAACGCGCGAGAAAAGTACCATCGATTCCAGATGTCCCGTGTGTGGGAACATATCCa > 1:3017133/1‑62 (MQ=255) tATTTAACGCGCGAGAGAAGTACCATCGATTCCAGATGTCCCGTGTGTGGGaa < 1:3928323/53‑1 (MQ=255) aTTTAACGCGCGAGAGAAGTACCATCGATTCCAGATGTcc > 1:939601/1‑40 (MQ=255) aTTTAACGCGCGAGAGAAGTAACATCGATTCCAGATGTCCCGTGTGTGGGAACATATCCAGCATCGCCAGt > 1:3577081/1‑71 (MQ=255) aTTTAACGCGCGAGAAAAGTACCATCGATTCCAGATGTCCCgtgtg > 1:3281225/1‑46 (MQ=255) aTTTAACGCGCGAGAAAAGTACCATCGATTCCAGATGTCCCgtgtg > 1:2152146/1‑46 (MQ=255) aTTTAACGCGCGAGAAAAGTACCATCGATTCCAGATGTCCCgtgtg > 1:3640327/1‑46 (MQ=255) aTTTAACGCGCGAGAAAAGTACCATCGATTCCAGATGTCCCGTGTGTGGGAACATATCCAGCATCGCCAGt > 1:1844611/1‑71 (MQ=255) aTTTAACGCGCGAGAAAAGTACCATCGATTCCAGATGTCCCGTGTGTGGGAACATATCCAGCATCGCCAGt > 1:2414187/1‑71 (MQ=255) aTTTAACGCGCGAGAAAAGTACCATCGATTCCAGATGTCCCGTGTGTGGGAACATATCCAGCATCGCCAGt > 1:3721528/1‑71 (MQ=255) aaCGCGCGAGAGAAGTACCATCGATTCCAGATGTCCCGTGTGTGGGAACATATCCAGCATCGCCAGTcgc < 1:1259808/70‑1 (MQ=255) aaCGCGCGAGAGAAGTACCATCGATTCCAGATGTCCCGTGTGTGGGAACATATCCAGCATCGCCAGTcgc < 1:809938/70‑1 (MQ=255) aaCGCGCGAGAAAAGTACCATCGATTCCAGATGTCCCGTGTGTGGGAACATATCCAGCATCGCCAGTcgcg > 1:1283976/1‑71 (MQ=255) gcgcgAGAAAAGTACCATCGATTCCAGCTGTCCCgtgtgt < 1:3813447/40‑1 (MQ=255) gcgcgAGAAAAGTACCATCGATTCCAGATGTCCCgtgtgt < 1:2723203/40‑1 (MQ=255) gcgcgAGAAAAGTACCATCGATTCCAGATGTCCCgtgtgt < 1:1123863/40‑1 (MQ=255) gcgAGAAAAGTACCATCGATTCCAGATGTCCCGTGTGTGGCAACATATCCAGCATCGCCAGTCGCGCAATg > 1:4141444/1‑71 (MQ=255) aaaaGTACCATCGATTCCAGATGTCCCGTGTGTGGGAACATATCCAGCATCGCCAGTCGCGCAATGGt < 1:2663417/68‑1 (MQ=255) aaaaGTACCATCGATTCCAGATGTCCCGTGTGTGGGAACATATCCAGCATCGCCAGTCGCGCAATGGt < 1:3820064/68‑1 (MQ=255) | AACCATCGTCCTCTCCTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAACTATTTAACGCGCGAGAAAAGTACCATCGATTCCAGATGTCCCGTGTGTGGGAACATATCCAGCATCGCCAGTCGCGCAATGGT > minE/1883128‑1883264 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |