| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,890,961 | G→A | 69.4% | P208S (CCG→TCG) | gudP ← | predicted D‑glucarate transporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,890,961 | 0 | G | A | 69.4% | 65.2 / 39.9 | 49 | P208S (CCG→TCG) | gudP | predicted D‑glucarate transporter |
| Reads supporting (aligned to +/- strand): ref base G (5/10); new base A (18/16); total (23/26) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.33e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TTGCTGATCCATATTGATCAGCGCACCACCCGCGGCGATGTACTCCAGCTCTTTCTTATTTACCCCCGGATGTTGATTTGGCTCGTGGATGACTTTCAACCAGATAAAGCTGATGACAATCCCCAGAC > minE/1890893‑1891020 | ttGCTGATCCATATTGATCAGCGCACCACCCGCGGCGATGTACTCCAGCTCTTTCTTATTTACCCCCGGa < 1:3305279/70‑1 (MQ=255)ttGCTGATCCATATTGATCAGCGCACCACCCGCGGCGATGTACTCCAGCTCTTTCTTATTTACCCCCGGa < 1:2310563/70‑1 (MQ=255) tCCATATTGATCAGCGCACCACCCGCGGCGATGTACTCCAGCTCTTTCTTATTTACCCCCGAATGTTGAt < 1:3390391/70‑1 (MQ=255) tatTGATCAGCGCACCACCCGCGGCGATGTACTCCAGCTCTTTCTTATTTACCCCCGAATGTTGATTTGGc > 1:766399/1‑71 (MQ=255) tatTGATCAGCGCACCACCCGCGGCGATGTACTCCAGCTCTTTCTTATTTACCCCCGAATGTTGATTTGGc > 1:1252820/1‑71 (MQ=255) tatTGATCAGCGCACCACCCGCGGCGATGTACTCCAGCTCTTTCTTATTTACCCCCGAATGTTGATTTGGc > 1:2031621/1‑71 (MQ=255) tatTGATCAGCGCACCACCCGCGGCGATGTACTCCAGCTCTTTCTTATTTACCCCCGAATGTTGATTTGGc > 1:2060957/1‑71 (MQ=255) ttGATCAGCGCACCACCCGCGGCGATGTACTCCAGCTCTTTCTTATTTACCCCCGGATGTTGATTTGGCTc > 1:738350/1‑71 (MQ=255) ttGATCAGCGCACCACCCGCGGCGATGTACTCCAGCTCTTTCTTATTTACCCCCGGATGTTGATTTGGCTc > 1:4244216/1‑71 (MQ=255) ttGATCAGCGCACCACCCGCGGCGATGTACTCCAGCTCTTTCTTATTTACCCCCGAATGTTGATTTGGCt < 1:2809255/70‑1 (MQ=255) ttGATCAGCGCACCACCCGCGGCGATGTACTCCAGCTCTTTCTTATTTACCCCCGAATGTTGATTTGGCTc > 1:1139546/1‑71 (MQ=255) ttGATCAGCGCACCACCCGCGGCGATGTACTCCAGCTCTTTCTTATTTACCCCCGAATGTTGATTTGGCTc > 1:4188039/1‑71 (MQ=255) ttGATCAGCGCACCACCCGCGGCGATGTACTCCAGCTCTTTCTTATTTACCCCCGAATGTTGATTTGGCTc > 1:3651435/1‑71 (MQ=255) ttGATCAGCGCACCACCCGCGGCGATGTACTCCAGCTCTTTCTTATTTACCCCCGAATGTTGATTTGGCTc > 1:190255/1‑71 (MQ=255) aTCAGCGCACCACCCGCGGCGATGTACTCCAGCTCTTTCTTATTTACCCCCGAATGTTGATTTGGCTCGTg < 1:987301/71‑1 (MQ=255) aTCAGCGCACCACCCGCGGCGATGTACTCCAGCTCTTTCTTATTTACCCCCGAATGTTGATTTGGCTCGTg < 1:2711960/71‑1 (MQ=255) aTCAGCGCACCACCCGCGGCGATGTACTCCAGCTCTTTCTTATTTACCCCCGAATGTTGATTTGGCTCGTg < 1:2179619/71‑1 (MQ=255) aTCAGCGCACCACCCGCGGCGATGTACTCCAGCTCTTTCTTATTTACCCCCGAAGGTTGATTTGGCTCGTg < 1:3732988/71‑1 (MQ=255) gcgcACCACCCGCGGCGATGTACTCCAGCTCTTTCTTATTTACCCCCGGATGTTGATTTGGCTCGTGGATg < 1:1898702/71‑1 (MQ=255) gcgcACCACCCGCGGCGATGTACTCCAGCTCTTTCTTATTTACCCCCGGATGTTGATTTGGCTCGTGGATg < 1:180146/71‑1 (MQ=255) cgcACCACCCGCGGCGATGTACTCCAGCTCTTTCTTATTTACCCCCGGATGTTGATTTGGCTCGTGGATg < 1:167243/70‑1 (MQ=255) caccCGCGGCGATGTACTCCAGCTCTTTCTTATTTACCCCCGGATGTTGATTTGGCTCGTGGATGACTTTc < 1:416127/71‑1 (MQ=255) accCGCGGCGATGTACTCCAGCTCTTTCTTATTTACCCCCGAATGTTGATTTGGCTCGTGGATGACTTTCa < 1:3782310/71‑1 (MQ=255) cccGCGGCGATGTACTCCAGCTCTTTCTTATTTACCCCCGGATGTTGATTTGGCTCGTg < 1:3374103/59‑1 (MQ=255) cccGCGGCGATGTACTCCAGCTCTTTCTTATTTACCCCCGGATGTTGATTTGGCTCGTGGATGACTTTCaa > 1:4020611/1‑71 (MQ=255) cccGCGGCGATGTACTCCAGCTCTTTCTTATTTACCCCCGAATGTTGATTTGGCTCGtt < 1:3219993/59‑2 (MQ=255) tACTCCAGCTCTTTCTTATTTACCCCCGGATGTTGATTTGGCTCGTGGATGACTTTCAACCAGATAAAGCt > 1:2783679/1‑71 (MQ=255) tACTCCAGCTCTTTCTTATTTACCCCCGGATGTTGATTTGGCTCGTGGATGACTTTCAACCAGATAAAGCt < 1:469009/71‑1 (MQ=255) aCTCCAGCTCTTTCTTATTTACCCCCGAATGTTGATTTGGCTCGTGGATGACtt > 1:1868096/1‑54 (MQ=255) ccAGCTCTTTCTTATTTACCCCCGAATGTTGATTTGGCTCGTGGATGACTTTCAACCAGATAAAGc > 1:1210315/1‑66 (MQ=255) cAGCTCTTTCTTATTTACCCCCGAATGTTGATTTGGCTCGTGGATGACTTTCAACCAGATAAAGCtgatga > 1:1027094/1‑71 (MQ=255) cAGCTCTTTCTTATTTACCCCCGAATGTTGATTTGGCTCGTGGATGACTTTCAACCAGATAAAGCtgatg < 1:3558120/70‑1 (MQ=255) cAGCTCTTTCTTATTTACCCCCGAATGTTGATTTGGCTCGTGGATGACTTTCAACCAGATAAAGCtgatg < 1:1280107/70‑1 (MQ=255) cAGCTCTTTCTTATTTACCCCCGAATGTTGATTTGGCTCGTGGATGACTTTCAACCAGATAAAGCtgatg < 1:3514757/70‑1 (MQ=255) gCTCTTTCTTATTTACCCCCGAATGTTGATTTGGCTCGTGGATGACTTTCAACCAGATAAAGCTGATGACa < 1:3231462/71‑1 (MQ=255) ctctTTCTTATTTACCCCCGAATGTTGATTTGGCTCGTGGATGAc > 1:3596497/1‑45 (MQ=255) ctctTTCTTATTTACCCCCGAATGTTGATTTGGCTCGTGGATGAc > 1:370118/1‑45 (MQ=255) ctctTTCTTATTTACCCCCGAATGTTGATTTGGCTCGTGGATGAc > 1:4206692/1‑45 (MQ=255) tttcttATTTACCCCCGGATGTTGATTTGGCTCGTGGATg < 1:1764310/40‑1 (MQ=255) tttcttATTTACCCCCGGATGTTGATTTGGCTCGTGGATg < 1:2976259/40‑1 (MQ=255) tcttATTTACCCCCGGATGTTGATTTGGCTCGTGGATGACTTTCAACCAGATAAAGCTGATGACAATccc > 1:1777746/1‑70 (MQ=255) tcttATTTACCCCCGAATGTTGATTTGGCTCGTGGATGACTTTCAACCAGATAAAGCTGATGACAATc < 1:3256431/68‑1 (MQ=255) tcttATTTACCCCCGAATGTTGATTTGGCTCGTGGATGACTTTCAACCAGATAAAGCTGATGACAATc < 1:3079609/68‑1 (MQ=255) cttATTTACCCCCGAATGTTGATTTGGCTCGTGGATGACTTTCAACCAGATAAAGCTGATGACAATccc > 1:3856114/1‑69 (MQ=255) aTTTACCCCCGAATGTTGATTTGGCTCGTGGATGACTTTCAACCAGATAAAGCTGATGACAATCCCCa < 1:1298944/68‑1 (MQ=255) aTTTACCCCCGAATGTTGATTTGGCTCGTGGATGACTTTCAACCAGATAAAGCTGATGACAATCCCCAGAc > 1:3625218/1‑71 (MQ=255) tttACCCCCGAATGTTGATTTGGCTCGTGGATGACTTTCAACCAGATAAAGCTGATGACAATCCCCa < 1:3512657/67‑1 (MQ=255) ttACCCCCGAATGTTGATTTGGCTCGTGGATGACTTTCAACCAGATAAAGCTGATGACaa > 1:2822508/1‑60 (MQ=255) ttACCCCCGAATGTTGATTTGGCTCGTGGATGACTTTCAACCAGATAAAGCTGATGACaa > 1:1806485/1‑60 (MQ=255) | TTGCTGATCCATATTGATCAGCGCACCACCCGCGGCGATGTACTCCAGCTCTTTCTTATTTACCCCCGGATGTTGATTTGGCTCGTGGATGACTTTCAACCAGATAAAGCTGATGACAATCCCCAGAC > minE/1890893‑1891020 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |