| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,914,848 | T→C | 56.8% | E208E (GAA→GAG) | ptsP ← | fused PEP‑protein phosphotransferase (enzyme I) of PTS system |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,914,848 | 0 | T | C | 56.8% | 16.5 / 43.6 | 37 | E208E (GAA→GAG) | ptsP | fused PEP‑protein phosphotransferase (enzyme I) of PTS system |
| Reads supporting (aligned to +/- strand): ref base T (7/9); new base C (15/6); total (22/15) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.07e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.60e-01 | |||||||||||
GCAAAGCGTTTGCTGTAGCGGCGAAACTCGTTTGCCGCTTCTTCCAGCGCCCCGGTCAGTCGTTCGCGTTCCAGAGCCGGATCCAGCGTTGATGCCTGATACACCTGTTCCATTAAAGGTAACGTGGCATCCTGC > minE/1914780‑1914914 | gcAAAGCGTTTGCTGTAGCGGCGAAACTCGTTTGCCGCTTCTTCCAGCGCCCCGGTCAGTCGTTCGCGTTc < 1:2218858/71‑1 (MQ=255) aGCGGCGAAACTCGTTTGCCGCTTCTTCCAGCGCCCCGGTCAGTCGTTCGCGTTCCAGAGCCGGATCCAg < 1:270757/70‑1 (MQ=255) aGCGGCGAAACTCGTTTGCCGCTTCTTCCAGCGCCCCGGTCAGTCGTTCGCGCTCCAGAGc > 1:469718/1‑61 (MQ=255) cggcgAAACTCGTTTGCCGCTTCTTCCAGCGCCCCGGTCAGTCGTTCGCGTTCCAGAGCCGGATCCAGCGt < 1:2930083/71‑1 (MQ=255) ggcgAAACTCGTTTGCCGCTTCTTCCAGCGCCCCGGTCAGTCGTTCGCGTTCCAGAGCCGGATCCAGCGtt < 1:157663/71‑1 (MQ=255) ggcgAAACTCGTTTGCCGCTTCTTCCAGCGCCCCGGTCAGTCGTTCGCGTTCCAGAGCCGGATCCAGCGtt < 1:2601684/71‑1 (MQ=255) ggcgAAACTCGTTTGCCGCTTCTTCCAGCGCCCCGGTCAGTCGTTCGCGTTCCAGAGCCGGATCCAGCGtt < 1:2357075/71‑1 (MQ=255) ggcgAAACTCGTTTGCCGCTTCTTCCAGCGCCCCGGTCAGTCGTTCGCGCTCCAGAGCCGGATCCAGCGtt < 1:1371325/71‑1 (MQ=255) ggcgAAACTCGTTTGCAGCTTCTTCCAGCGCCCCGGTCAGTCGTTCGCGCTCCAGAGCCGGATCCAGCGtt < 1:3176555/71‑1 (MQ=255) cgAAACTCGTTTGCCGCTTCTTCCAGCGCCCCGGTCAGTCGTTCGCGCTCCAGAGCCGGATCCAGCGTTGa > 1:2208824/1‑71 (MQ=255) gAAACTCGTTTGCCGCTTCTTCCAGCGCCCCGGTCAGTCGTTCGCGCTCCAGAGCCGGATCCAGCGTTGAt > 1:3546388/1‑71 (MQ=255) aaaCTCGTTTGCCGCTTCTTCCAGCGCCCCGGTCAGTCGTTCGCGTTCCAGAGCCGGATCCAGCGtt < 1:1393958/67‑1 (MQ=255) aaaCTCGTTTGCCGCTTCTTCCAGCGCCCCGGTCAGTCGTTCGCGTTCCAGAGCCGGATCCAGCGtt < 1:4233492/67‑1 (MQ=255) aCTCGTTTGCCGCTTCTTCCAGCGCCCCGGTCAGTCGTTCGCGTTCCAGAGCCGGATCCAGCGTTGa > 1:807336/1‑67 (MQ=255) aCTCGTTTGCCGCTTCTTCCAGCGCCCCGGTCAGTCGTTCGCGTTCCAGAGCCGGATCCAGCGTTGa > 1:4158726/1‑67 (MQ=255) aCTCGTTTGCCGCTTCTTCCAGCGCCCCGGTCAGTCGTTCGCGCTCCAGAGCCGGATCCAGCGtt < 1:2673209/65‑1 (MQ=255) gTTTGCCGCTTCTTCCAGCGCCCCGGTCAGTCGTTCGCGTTCCAGAGCCGGATCCAGCGtt > 1:578220/1‑61 (MQ=255) gTTTGCCGCTTCTTCCAGCGCCCCGGTCAGTCGTTCGCGTTCCAGAGCCGGATCCAGCGtt > 1:3909709/1‑61 (MQ=255) gTTTGCCGCTTCTTCCAGCGCCCCGGTCAGTCGTTCGCGCTcc > 1:2351428/1‑43 (MQ=255) gTTTGCCGCTTCTTCCAGCGCCCCGGTCAGTCGTTCGCGCTcc > 1:2536815/1‑43 (MQ=255) gTTTGCCGCTTCTTCCAGCGCCCCGGTCAGTCGTTCGCGCTcc > 1:945773/1‑43 (MQ=255) gTTTGCCGCTTCTTCCAGCGCCCCGGTCAGTCGTTCGCGCTcc > 1:66743/1‑43 (MQ=255) gTTTGCCGCTTCTTCCAGCGCCCCGGTCAGTCGTTCGCGCTcc > 1:565610/1‑43 (MQ=255) gCTTCTTCCAGCGCCCCGGTCAGTCGTTCGCGTTCCAGAGCCGGATCCAGCGTTGATGCCTGATACACCTg > 1:903593/1‑71 (MQ=255) ttcttcCAGCGCCCCGGTCAGTCGTTCGCGTTCCAGAGCCGGATCCAGCGtt < 1:2518895/52‑1 (MQ=255) cttcCAGCGCCCCGGTCAGTCGTTCGCGTTCCAGAGCCGGATCCAGCGTTGATGCCTGATACACCTGTTc > 1:101830/1‑70 (MQ=255) cttcCAGCGCCCCGGTCAGTCGTTCGCGTTCCAGAGCCGGATCCAGCGTTGATGCCTGATACACCTGTTc > 1:935547/1‑70 (MQ=255) cGGTCAGTCGTTCGCGCTCCAGAGCCGGATCCAGCGTTGa > 1:47729/1‑40 (MQ=255) cGGTCAGTCGTTCGCGCTCCAGAGCCGGATCCAGCGTTGa > 1:763849/1‑40 (MQ=255) cGGTCAGTCGTTCGCGCTCCAGAGCCGGATCCAGCGTTGATGCCTGa > 1:3521917/1‑47 (MQ=255) cGGTCAGTCGTTCGCGCTCCAGAGCCGGATCCAGCGTTGATGCCTGa > 1:1969137/1‑47 (MQ=255) cAGTCGTTCGCGCTCCAGAGCCGGATCCAGCGTTGATGCCTGATACACCTGTTCCATTAAAGGTAACGTgg > 1:2549104/1‑71 (MQ=255) gTCGTTCGCGCTCCAGAGCCGGATCCAGCGTTGATGCCTGATACACCTGTTCCATTAAAGGTAACGTGGCa < 1:3234189/71‑1 (MQ=255) tCGTTCGCGCTCCAGAGCCGGATCCAGCGTTGATGCCTGATACACCTGTTCCATTa < 1:2876402/56‑1 (MQ=255) tCGTTCGCGCTCCAGAGCCGGATCCAGCGTTGATGCCTGATACACCTGTTCCATTa < 1:949908/56‑1 (MQ=255) cgcgCTCCAGAGCCGGATCCAGCGTTGATGCCTGATACACCTGTTCCATTAAAGGTAACGTGGCATCCTg > 1:386556/1‑70 (MQ=255) cgcgCTCCAGAGCCGGATCCAGCGTTGATGCCTGATACACCTGTTCCATTAAAGGTAACGTGGCATCCTGc > 1:2095325/1‑71 (MQ=255) | GCAAAGCGTTTGCTGTAGCGGCGAAACTCGTTTGCCGCTTCTTCCAGCGCCCCGGTCAGTCGTTCGCGTTCCAGAGCCGGATCCAGCGTTGATGCCTGATACACCTGTTCCATTAAAGGTAACGTGGCATCCTGC > minE/1914780‑1914914 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |