BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	minE	1,993,572	G→A	42.6%	V289M (GTG→ATG)	nupG →	nucleoside transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	1,993,572	0	G	A	42.6%	55.2 / 128.5	101	V289M (GTG→ATG)	nupG	nucleoside transporter
Reads supporting (aligned to +/- strand): ref base G (24/34); new base A (24/19); total (48/53)
Fisher's exact test for biased strand distribution p-value = 1.64e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.22e-01

Only 100 of 103 total aligned reads displayed.

ATTCTGACCATCCCGTTCTTCTTAAGCCGCTACGGTATTAAGAACGTAATGATGATCAGTATTGTGGCGTGGATCCTGCGTTTTGCGCTGTTTGCTTACGGCGACCCGACTCCGTTCGGTACTGTACTGCTGG  >  minE/1993509‑1993641

aTTCTGACCATCCCGTTCTTCTTAAGCCGCTACGGTATTAAGAACGTAATGATGATCAGTATTATGGCGTg                                                                >  1:3511809/1‑71 (MQ=255)

aTTCTGACCATCCCGTTCTTCTTAAGCCGCTACGGTATTAAGAACGTAATGATGATCAGTATTATGGCGTg                                                                >  1:1909186/1‑71 (MQ=255)

  tCTGACCATCCCGTTCTTCTTAAGCCGCTACGGTATTAAGAACGTAATGATGATCAGTATTATGGCGTGGa                                                              >  1:2884741/1‑71 (MQ=255)

   cTGACCATCCCGTTCTTCTTAAGCCGCTACGGTATTAAGAACGTAATGATGATCAGTATTGTGGc                                                                   >  1:446910/1‑65 (MQ=255)

   cTGACCATCCCGTTCTTCTTAAGCCGCTACGGTATTAAGAACGTAATGATGATCAGTATTGTGGCGTGGa                                                              >  1:3469520/1‑70 (MQ=255)

   cTGACCATCCCGTTCTTCTTAAGCCGCTACGGTATTAAGAACGTAATGATGATCAGTATTGTGGCGTGGAt                                                             >  1:3687625/1‑71 (MQ=255)

   cTGACCATCCCGTTCTTCTTAAGCCGCTACGGTATTAAGAACGTAATGATGATCAGTATTATGGCGTGGAt                                                             >  1:1072131/1‑71 (MQ=255)

   cTGACCATCCCGTTCTTCTTAAGCCGCTACGGTATTAAGAACGTAATGATGATCAGTATTAAGGCGTGGAt                                                             >  1:3477134/1‑71 (MQ=255)

     gACCATCCCGTTCTTCTTAAGCCGCTACGGTATTAAGAACGTAATGATGATCAGTATTGTGGCGTGGATcc                                                           >  1:4212386/1‑71 (MQ=255)

     gACCATCCCGTTCTTCTTAAGCCGCTACGGTATTAAGAACGTAATGATGATCAGTATTGTGGCGTGGATcc                                                           >  1:4046264/1‑71 (MQ=255)

      aCCATCCCGTTCTTCTTAAGCCGCTACGGTATTAAGAACGTAATGATGATCAGTATTATGGCGTGGATc                                                            <  1:1102278/69‑1 (MQ=255)

       ccATCCCGTTCTTCTTAAGCCGCTACGGTATTAAGAACGTAATGATGATCAGTATTATGGCGTGGATCCt                                                          >  1:3995844/1‑70 (MQ=255)

        cATCCCGTTCTTCTTAAGCCGCTACGGTATTAAGAACGTAATGATGATCAGTATTGTGGCGTGGATCCTGc                                                        <  1:3408409/71‑1 (MQ=255)

        cATCCCGTTCTTCTTAAGCCGCTACGGTATTAAGAACGTAATGATGATCAGTATTGTGGCGTGGATCCTGc                                                        <  1:106442/71‑1 (MQ=255)

          tCCCGTTCTTCTTAAGCCGCTACGGTATTAAGAACGTAATGATGATCAGTATTGTGGCGTGGATCCTGCGt                                                      >  1:2715320/1‑71 (MQ=255)

          tCCCGTTCTTCTTAAGCCGCTACGGTATTAAGAACGTAATGATGATCAGTATTGTGGCGTGGATCCTGCGt                                                      >  1:4035848/1‑71 (MQ=255)

          tCCCGTTCTTCTTAAGCCGCTACGGTATTAAGAACGTAATGATGATCAGTATTATGGCGTGGAt                                                             <  1:1848729/64‑1 (MQ=255)

            ccGTTCTTCTTAAGCCGCTACGGTATTAAGAACGTAATGATGATCAGTATTATGGCGTGGATCCTGCGttt                                                    >  1:733254/1‑71 (MQ=255)

            ccGTTCTTCTTAAGCCGCTACGGTATTAAGAACGTAATGATGATCAGTATTATGGCGTGGATCCTGCGttt                                                    >  1:1678534/1‑71 (MQ=255)

            ccGTTCTTCTTAAGCCGCTACGGTATTAAGAACGTAATGATGATCAGTATTATGGCGTGGATCCTGCGttt                                                    >  1:2280080/1‑71 (MQ=255)

            ccGTTCTTCTTAAGCCGCTACGGTATTAAGAACGTAATGATGATCAGTATTATGGCGTCGATCCTGCGttt                                                    >  1:2245995/1‑71 (MQ=255)

               ttcttcTTAAGCCGCTACGGTATTAAGAACGTAATGATGATCAGTATTGTGGCGTGGATCCTGCGTTTTgc                                                 >  1:1635139/1‑71 (MQ=255)

               ttcttcTTAAGCCGCTACGGTATTAAGAACGTAATGATGATCAGTATTGTGGCGTGGATCCTGCGTTTTgc                                                 >  1:3270217/1‑71 (MQ=255)

               ttcttcTTAAGCCGCTACGGTATTAAGAACGTAATGATGATCAGTATTGTGGCGTGGATCCTGCGTTTTgc                                                 >  1:2106119/1‑71 (MQ=255)

               ttcttcTTAAGCCGCTACGGTATTAAGAACGTAATGATGATCAGTATTGTGGCGTGGATCCTGCGTTTTgc                                                 >  1:891647/1‑71 (MQ=255)

                 cttcttAAGCCGCTACGGTATTAAGAACGTAATGATGATCAGTATTGTGGCGTGGa                                                              <  1:2772830/56‑1 (MQ=255)

                 cttcttAAGCCGCTACGGTATTAAGAACGTAATGATGATCAGTATTGTGGCGTGGATCCTGCGTTTTgcgc                                               >  1:3460472/1‑71 (MQ=255)

                  ttcttAAGCCGCTACGGTATTAAGAACGTAATGATGATCAGTATTATGGCGTGGATCCTGCGTTTTgc                                                 <  1:2256023/68‑1 (MQ=255)

                  ttcttAAGCCGCTACGGTATTAAGAACGTAATGATGATCAGTATTATGGCGTGGATCCTGCGTTTTgc                                                 <  1:3537840/68‑1 (MQ=255)

                   tcttAAGCCGCTACGGTATTAAGAACGTAATGATGATCAGTATTGTGGCGTGGATc                                                            >  1:3809687/1‑56 (MQ=255)

                    cttAAGCCGCTACGGTATTAAGAACGTAATGATGATCAGTATtatg                                                                     >  1:92021/1‑46 (MQ=255)

                    cttAAGCCGCTACGGTATTAAGAACGTAATGATGATCAGTATTATGGCGTGGATCCTGCGTTTTgcgc                                               <  1:3332840/68‑1 (MQ=255)

                    cttAAGCCGCTACGGTATTAAGAACGTAATGATGATCAGTATTATGGCGTGGATCCTGCGTTTTgcgc                                               >  1:2610911/1‑68 (MQ=255)

                    cttAAGCCGCTACGGTATTAAGAACGTAATGATGATCAGTATTATGGCGTGGATCCTGCGTTTTgcgc                                               >  1:197570/1‑68 (MQ=255)

                    cttAAGCCGCTACGGTATTAAGAACGTAATGATGATCAGTATTATGGCGTGGATCCTGCGTTTTgcgc                                               >  1:1011995/1‑68 (MQ=255)

                    cttAAGCCGCTACGGTATTAAGAACGTAATGATGATCAGTATTATGGCGTGGATCCTGCGTTTTgcgc                                               <  1:2766439/68‑1 (MQ=255)

                    cttAAGCCGCTACGGTATTAAGAACGTAATGATGATCAGTATTATGGCGTGGATCCTGCGTTTTgcgc                                               >  1:3651997/1‑68 (MQ=255)

                    cttAAGCCGCTACGGTATTAAGAACGTAATGATGATCAGTATTATGGCGTGGATCCTGCGTTTTgcgc                                               <  1:3712335/68‑1 (MQ=255)

                    cttAAGCCGCTACGGTATTAAGAACGTAATGATGATCAGTATTATGGCGTGGATCCTGCGTTTTgcgc                                               >  1:4132653/1‑68 (MQ=255)

                    cttAAGCCGCTACGGTATTAAGAACGTAATGATGATCAGTATTATGGCGTGGATCCTGCGTTTTgcgc                                               >  1:630952/1‑68 (MQ=255)

                      tAAGCCGCTACGGTATTAAGAACGTAATGATGATCAGTATTATGGCGTGGATCCTGCGTTTTGCGCTGttt                                          >  1:4121180/1‑71 (MQ=255)

                       aaGCCGCTACGGTATTAAGAACGTAATGATGATCAGTATTGTGGCGTGGATCCTGCGTTTTGCGCTGt                                            <  1:3306552/68‑1 (MQ=255)

                       aaGCCGCTACGGTATTAAGAACGTAATGATGATCAGTATTGTGGCGTGGATCCTGCGTTTTGCGCTGt                                            <  1:4194253/68‑1 (MQ=255)

                       aaGCCGCTACGGTATTAAGAACGTAATGATGATCAGTATTGTGGCGTGGATCCTGCGTTTTGCGCTGt                                            <  1:465700/68‑1 (MQ=255)

                       aaGCCGCTACGGTATTAAGAACGTAATGATGATCAGTATTGTGGCGTGGATCCTGCGTTTTGCGCTGt                                            <  1:3031256/68‑1 (MQ=255)

                       aaGCCGCTACGGTATTAAGAACGTAATGATGATCAGTATTGTGGCGTGGATCCTGCGTTTTGCGCTGt                                            <  1:213114/68‑1 (MQ=255)

                       aaGCCGCTACGGTATTAAGAACGTAATGATGATCAGTATTATGGCGTGGATCCTGCGTTTTGCGCTGt                                            <  1:3767293/68‑1 (MQ=255)

                        aGCCGCTACGGTATTAAGAACGTAATGATGATCAGTATTATGGCGTGGATCCTGCGTTTTGCGCTGTTTg                                         <  1:1563873/70‑1 (MQ=255)

                         gCCGCTACGGTATTAAGAACGTAATGATGATCAGTATTGTGGCGTGGATCCTGCGTTTTGCTCTGt                                            <  1:1921215/66‑1 (MQ=255)

                            gCTACGGTATTAAGAACGTAATGATGATCAGTATTATGGCGTGGATCCTGCGTTTTGCGCTGTTTGCTTAc                                    <  1:2501223/71‑1 (MQ=255)

                             cTACGGTATTAAGAACGTAATGATGATCAGTATTGTGGCGTGGATCCTGc                                                        <  1:1555218/50‑1 (MQ=255)

                             cTACGGTATTAAGAACGTAATGATGATCAGTATTGTGGCGTGGATCCTGCGTTTTgc                                                 <  1:275785/57‑1 (MQ=255)

                             cTACGGTATTAAGAACGTAATGATGATCAGTATTGTGGCGTGGATCCTGCGTTTTGCGCTGt                                            <  1:1510960/62‑1 (MQ=255)

                             cTACGGTATTAAGAACGTAATGATGATCAGTATTGTGGCGTGGATCCTGCGTTTTGCGCTGt                                            <  1:224908/62‑1 (MQ=255)

                             cTACGGTATTAAGAACGTAATGATGATCAGTATTGTGGCGTGGATCCTGCGTTTTGCGCTGt                                            <  1:37637/62‑1 (MQ=255)

                             cTACGGTATTAAGAACGTAATGATGATCAGTATTGTGGCGTGGATCCTGCGTTTTGCGCTGt                                            <  1:1456055/62‑1 (MQ=255)

                             cTACGGTATTAAGAACGTAATGATGATCAGTATTGTGGCGTGGATCCTGCGTTTTGCGCTGt                                            <  1:2922490/62‑1 (MQ=255)

                              tACGGTATTAAGAACGTAATGATGATCAGTATTATGGCGTGGATCCTGCGTTTTGCGCTGTTTGCTTACgg                                  >  1:3121932/1‑71 (MQ=255)

                              tACGGTATTAAGAACGTAATGATGATCAGTATTATGGCGTGGATCCTGCGTTTTGCGCTGTTTGCTTACgg                                  >  1:136367/1‑71 (MQ=255)

                              tACGGTATTAAGAACGTAATGATGATCAGTATTATGGCGTGGATCCTGCGTTTTGCGCTGTTTGCTTACgg                                  >  1:2089028/1‑71 (MQ=255)

                               aCGGTATTAAGAACGTAATGATGATCAGTATTATGGCGTGGATCCTGCGTTTTGCGCTGTTTGCTTACGGc                                 >  1:3503209/1‑71 (MQ=255)

                               aCGGTATTAAGAACGTAATGATGATCAGTATTATGGCGTGGATCCTGCGTTTTGCGCTGTTTGCTTACGGc                                 >  1:2845914/1‑71 (MQ=255)

                                     ttAAGAACGTAATGATGATCAGTATTGTGGCGTGGa                                                              <  1:3186231/36‑1 (MQ=255)

                                     ttAAGAACGTAATGATGATCAGTATTGTGGCGTGGATCCTGCGTTTTGCGCTGTTTGCt                                       <  1:2912672/59‑1 (MQ=255)

                                     ttAAGAACGTAATGATGATCAGTATTGTGGCGTGGATCCTGCGTTTTGCGCTGTTTGCt                                       <  1:365099/59‑1 (MQ=255)

                                     ttAAGAACGTAATGATGATCAGTATTGTGGCGTGGATCCTGCGTTTTGCGCTGTTTGCt                                       <  1:3536648/59‑1 (MQ=255)

                                     ttAAGAACGTAATGATGATCAGTATTGTGGCGTGGATCCTGCGTTTTGCGCTGTTTGCTTACGGCGAccc                            <  1:14488/70‑1 (MQ=255)

                                     ttAAGAACGTAATGATGATCAGTATTGTGGCGTGGATCCTGCGTTTTGCGCTGTTTGCTTACGGCGAccc                            <  1:1234110/70‑1 (MQ=255)

                                      tAAGAACGTAATGATGATCAGTATTGTGGCGTGGATCCTGCGTTTTGCGCTGTTTGCt                                       <  1:3192029/58‑1 (MQ=255)

                                       aaGAACGTAATGATGATCAGTATTGTGGCGTGGAt                                                             >  1:3661259/1‑35 (MQ=255)

                                       aaGAACGTAATGATGATCAGTATTGTGGCGTGGATc                                                            >  1:1838450/1‑36 (MQ=255)

                                       aaGAACGTAATGATGATCAGTATTGTGGCGTGGATc                                                            >  1:3898314/1‑36 (MQ=255)

                                       aaGAACGTAATGATGATCAGTATTATGGCGTGGATCCTGCGTTTTGCGCTGTTTGCTTACGGCGACCCGa                          <  1:2869545/70‑1 (MQ=255)

                                        aGAACGTAATGATGATCAGTATTGTGGCGTGGATCCTGCGTTTTGCGCTGTTTGCtt                                      <  1:2226067/57‑1 (MQ=255)

                                        aGAACGTAATGATGATCAGTATTGTGGCGTGGATCCTGCGTTTTGCGCTGTTTGCtt                                      <  1:2389269/57‑1 (MQ=255)

                                        aGAACGTAATGATGATCAGTATTGTGGCGTGGATCCTGCGTTTTGCGCTGTTTGCtt                                      <  1:438605/57‑1 (MQ=255)

                                        aGAACGTAATGATGATCAGTATTGTGGCGTGGATCCTGCGTTTTGCGCTGTTTGCtt                                      <  1:3277141/57‑1 (MQ=255)

                                        aGAACGTAATGATGATCAGTATTGTGGCGTGGATCCTGCGTTTTGCGCTGTTTGCtt                                      <  1:2698318/57‑1 (MQ=255)

                                         gAACGTAATGATGATCAGTATTATGGCGTGGATCCTGCGTTTTgc                                                 <  1:1674008/45‑1 (MQ=255)

                                             ttAATGATGATCAGTATTGTGGCGTGGATCCTGCGTTTTGCGCTGttt                                          >  1:1265532/2‑48 (MQ=255)

                                             gTAATGATGATCAGTATTGTGGCGTGGATCCTGCGTTTTGCGCTGttt                                          >  1:3668496/1‑48 (MQ=255)

                                             gTAATGATGATCAGTATTGTGGCGTGGATCCTGCGTTTTGCGCTGttt                                          >  1:1270540/1‑48 (MQ=255)

                                             gTAATGATGATCAGTATTGTGGCGTGGATCCTGCGTTTTGCGCTGTTTGCTTa                                     <  1:929690/53‑1 (MQ=255)

                                             gTAATGATGATCAGTATTGTGGCGTGGATCCTGCGTTTTGCGCTGTTTGCTTa                                     <  1:2923164/53‑1 (MQ=255)

                                             gTAATGATGATCAGTATTGTGGCGTGGATCCTGCGTTTTGCGCTGTTTGCTTa                                     <  1:4070772/53‑1 (MQ=255)

                                              tAATGATGATCAGTATTATGGCGTGGATCCTGCGTTTTGCCCTGTTTGCTTACGGCGACCCGACTCCGTTc                  <  1:3533414/71‑1 (MQ=255)

                                                atgatgATCAGTATTGTGGCGTGGATCCTGCGTTTTGCGCTGTTTGCTTACGGCGACCCGACTCCGTTCgg                >  1:4257261/1‑71 (MQ=255)

                                                atgatgATCAGTATTGTGGCGTGGATCCTGCGTTTTGCGCTGTTTGCTTACGGCGACCCGACTCCGTTCgg                >  1:675509/1‑71 (MQ=255)

                                                 tgatgaTCATTATTGTGGCGTGGATCCTGCGTTTTGCGCTg                                             <  1:1526401/41‑1 (MQ=255)

                                                 tgatgaTCAGTATTGTGGCGTGGATCCTGCGTTTTGCGCTg                                             <  1:3088495/41‑1 (MQ=255)

                                                 tgatgaTCAGTATTGTGGCGTGGATCCTGCGTTTTGCGCTg                                             <  1:2313081/41‑1 (MQ=255)

                                                   atgatCAGTATTATGGCGTGGATCCTGCGTTTTGCGCTGTTTGCTTACGGCGACCCGACTCCGTTCGgta              <  1:1501671/70‑1 (MQ=255)

                                                    tgatCAGTATTATGGCGTGGATCCTGCGTTTTGCGCTGTTTGCTTACGGCGACCCGACTCCGTTc                  <  1:3111855/65‑1 (MQ=255)

                                                    tgatCAGTATTATGGCGTGGATCCTGCGTTTTGCGCTGTTTGCTTACGGCGACCCGACTCCGTTc                  <  1:735938/65‑1 (MQ=255)

                                                      atCAGTATTATGGCGTGGATCCTGCGTTTTGCGCTGTTTGCTTACGGCGACCCGACTCCGTTCGgtactg           <  1:2667991/70‑1 (MQ=255)

                                                      atCAGTATTATGGCGTGGATCCTGCGTTTTGCGCTGTTTGCTTACGGCGACCCGACTCCGTTCGgtactg           <  1:1722309/70‑1 (MQ=255)

                                                      atCAGTATTATGGCGTGGATCCTGCGTTTTGCGCTGTTTGCTTACGGCGACCCGACTCCGTTCGgtactg           <  1:2442117/70‑1 (MQ=255)

                                                             ttGTGGCGTGGATCCTGCGTTTTGCGCTGTTTGCTTACGGCGACCCGACTCCGTTCGGTACTGTActgctg   >  1:1523393/1‑71 (MQ=255)

                                                             ttGTGGCGTGGATCCTGCGTTTTGCGCTGTTTGCTTACGGCGACCCGACTCCGTTCGGTACTGTActgctg   >  1:3152992/1‑71 (MQ=255)

                                                               gtgGCGTGGATCCTGCGTTTTGCGCTGTTTGCTTACGGCGACCCGACTCCGTTCGGTACTGTACTGCTgg  <  1:736865/70‑1 (MQ=255)

ATTCTGACCATCCCGTTCTTCTTAAGCCGCTACGGTATTAAGAACGTAATGATGATCAGTATTGTGGCGTGGATCCTGCGTTTTGCGCTGTTTGCTTACGGCGACCCGACTCCGTTCGGTACTGTACTGCTGG  >  minE/1993509‑1993641

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`