| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,065,298 | T→C | 7.8% | D513D (GAT→GAC) | rpoD → | RNA polymerase, sigma 70 (sigma D) factor |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,065,298 | 0 | T | C | 7.8% | 287.7 / 14.4 | 102 | D513D (GAT→GAC) | rpoD | RNA polymerase, sigma 70 (sigma D) factor |
| Reads supporting (aligned to +/- strand): ref base T (58/36); new base C (4/4); total (62/40) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.08e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01 | |||||||||||
Only 100 of 118 total aligned reads displayed.
AAGACAAGATCCGCAAAGTGCTGAAGATCGCCAAAGAGCCAATCTCCATGGAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAGGATACCACCCTCGAGCTGCCGCTGGATTCTG > minE/2065231‑2065362 | aaGACAAGATCCGCAAAGTGCTGAAGATCGCCAAAGAGCCAATCTCCATGGAAACGCCGATCGGtgatgat > 1:2890412/1‑71 (MQ=255)aaGACAAGATCCGCAAAGTGCTGAAGATCGCCAAAGAGCCAATCTCCATGGAAACGCCGATCGGtgatgat > 1:506155/1‑71 (MQ=255)aaGACAAGATCCGCAAAGTGCTGAAGATCGCCAAAGAGCCAATCTCCATGGAAACGCCGATCGGtgatga > 1:4198503/1‑70 (MQ=255)aaGACAAGATCCGCAAAGTGCTGAAGATCGCCAAAGAGCCAATCTCCATGGAAACGCCGATCGGtgatga > 1:2302599/1‑70 (MQ=255)aaGACAAGATCCGCAAAGTGCTGAAGATCGCCAAAGAGCCAATCTCCATGGAAACGCCGATCGGtgatga > 1:2523365/1‑70 (MQ=255)aaGACAAGATCCGCAAAGTGCTGAAGATCGCCAAAGAGCCAATCTCCATGGAAACGCCGATCGGtgatga > 1:3747974/1‑70 (MQ=255) aGACAAGATCCGCAAAGTGCTGAAGATCGCCAAAGAGCCAATCTCCATGTAAACGCCGATCGGtgatgatg < 1:3166257/71‑1 (MQ=255) aGACAAGATCCGCAAAGTGCTGAAGATCGCCAAAGAGCCAATCTCCATGGAAACGCCGATCGGtgatgatg < 1:2288219/71‑1 (MQ=255) aCAAGATCCGCAAAGTGCTGAAGATCGCCAAAGAGCCAATCTCCATGGAAACGCCGATCGGTGATGATGaa < 1:2755709/71‑1 (MQ=255) aCAAGATCCGCAAAGTGCTGAAGATCGCCAAAGAGCCAATCTCCATGGAAACGCCGATCGGTGATGATGaa < 1:562301/71‑1 (MQ=255) aCAAGATCCGCAAAGTGCTGAAGATCGCCAAAGAGCCAATCTCCATGGAAACGCCGATCGGTGATGATGaa < 1:3557579/71‑1 (MQ=255) aCAAGATCCGCAAAGTGCTGAAGATCGCCAAAGAGCCAATCTCCATGGAAACGCCGATCGGTGATGATGaa < 1:1880114/71‑1 (MQ=255) aaGATCCGCAAAGTGCTGAAGATCGCCAAAGAGCCAATCTCCATGGAAACGCCGATCGGtgatgatg > 1:1888100/1‑67 (MQ=255) aaGATCCGCAAAGTGCTGAAAATCGCCAAAGAGCCAATCTCCAAGGAAACGCCGATCGGtgatgatg > 1:3870036/1‑67 (MQ=255) aGATCCGCAAAGTGCTGAAGATCGCCAAAGAGCCAATCTCCATGGAAACGCCGATCGGTGATGATGAAGAt < 1:722895/71‑1 (MQ=255) gATCCGCAAAGTGCTGAAGATCGCCAAAGAGCCAATCTCCATGGAAACGCCGATCGGTGATGATGAAGAtt < 1:624232/71‑1 (MQ=255) tcagCAAAGTGCTGAAGATCGCCAAAGAGCCAATCTCCATGGAAACGCCGATCGGTGATGATGAAGATTCg > 1:1060041/4‑71 (MQ=255) tCCGCAAAGTGCTGAAGATCGCCAAAGAGCCAATCTCCATGGAAACGCCGATCGGTGATGATGAAGATTCg > 1:2300954/1‑71 (MQ=255) tCCGCAAAGTGCTGAAGATCGCCAAAGAGCCAATCTCCATGGAAACGCCGATCGGTGATGATGAAGATTCg > 1:2728218/1‑71 (MQ=255) tCCGCAAAGTGCTGAAGATCGCCAAAGAGCCAATCTCCATGGAAACGCCGATCGGTGATGATGAAGATTCg > 1:1088184/1‑71 (MQ=255) aaaGTGCTGAAGATCGCCAAAGAGCCAATCTCCATGGAAACGCCGATCGGTGATGATGAAGATTCGCATCt < 1:218585/71‑1 (MQ=255) aaaGTGCTGAAGATCGCCAAAGAGCCAATCTCCATGGAAACGCCGATCGGTGATGATGAAGATTCGCATCt < 1:2307002/71‑1 (MQ=255) tGCTGAAGATCGCCAAAGAGCCAATCTCCATGGAAACGCCGATCGGTGATGATGAAGATTCGCATCTgggg < 1:794942/71‑1 (MQ=255) atGAAGATTGCGAAAGAGCCAATCTCCATGGAAACGCCGATCGGTGACGACGACGATTCGCATCTGGGCGa < 1:539733/70‑1 (MQ=255) atGAAGATTGCGAAAGAGCCAATCTCCATGGAAACGCCGATCGGTGACGACGACGATTCGCATCTGGGCGa < 1:2734669/70‑1 (MQ=255) tGAAGATTGCGAAAGAGCCAATCTCCATGGAAACGCCGATCGGTGACGACGACGATTCGCATCTGGGCGa < 1:2835631/70‑1 (MQ=255) gAAGATTGCGAAAGAGCCAATCTCCATGGAAACGCCGATCGGTGACGACGACGATTCGCATCTGGGCGa < 1:1103652/69‑1 (MQ=255) gATCGCCAAAGAGCCAATCTCCATGGAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCa > 1:1906674/1‑71 (MQ=255) cGCCAAAGAGCCAATCTCCATGGAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATc > 1:4160679/1‑70 (MQ=255) cGCCAAAGAGCCAATCTCCATGGAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATc > 1:1376201/1‑70 (MQ=255) cGCCAAAGAGCCAATCTCCATGGAAACGCCGATCGGTGATGATGAAGATTCGCATCT‑GGGGATTTCATc > 1:2489449/1‑69 (MQ=255) gCCAAAGAGCCAATCTCCATGGAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCg < 1:2138166/70‑1 (MQ=255) ccAAAGAGCCAATCTCCATGGAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAg < 1:258792/71‑1 (MQ=255) aaaGAGCCAATCTCCATGGAAACTCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAGGa < 1:1478647/71‑1 (MQ=255) aaaGAGCCAATCTCCATGGAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAGGa < 1:2056829/71‑1 (MQ=255) aaaGAGCCAATCTCCATGGAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAGGa < 1:187460/71‑1 (MQ=255) aaaGAGCCAATCTCCATGGAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAGGa < 1:4061185/71‑1 (MQ=255) aaGAGCCAATCTCCATGGAAACGCCGATCGGTGATGATGAAGATTCGCATCTg < 1:1005285/53‑1 (MQ=255) aaGAGCCAATCTCCATGGAAACGCCGATCGGTGATGATGAAGATTCGCATCTg < 1:1843075/53‑1 (MQ=255) aaGAGCCAATCTCCATGGAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAGGa > 1:1766298/1‑70 (MQ=255) aaGAGCCAATCTCCATGGAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAGGa > 1:4015882/1‑70 (MQ=255) aaGAGCCAATCTCCATGGAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAGGAt < 1:2413665/71‑1 (MQ=255) aaGAGCCAATCTCCATGGAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAGGAt < 1:206824/71‑1 (MQ=255) aaGAGCCAATCTCCATGGAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAGGAt < 1:182618/71‑1 (MQ=255) aaGAGCCAATCTCCATGGAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAGGAt < 1:3433476/71‑1 (MQ=255) gagCCAATCTCCATGGAAACGCCGATCGGTGATGATGAAGATTCGCATCTggg > 1:3158147/1‑53 (MQ=255) gagCCAATCTCCATGGAAACGCCGATCGGTGATGATGAAGATTCGCATCTggg > 1:2621536/1‑53 (MQ=255) gagCCAATCTCCATGGAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAg > 1:2325880/1‑66 (MQ=255) gagCCAATCTCCATGGAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAg > 1:2764132/1‑66 (MQ=255) gagCCAATCTCCATGGAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAg > 1:2749768/1‑66 (MQ=255) gagCCAATCTCCATGGAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAg > 1:1900769/1‑66 (MQ=255) gagCCAATCTCCATGGAAACGCCGATCGGTGACGACGACGATTCGCATCTGGGCGATTTTATCGAGGa > 1:1687634/1‑68 (MQ=255) gagCCAATCTCCATGGAAACGCCGATCGGTGACGACGACGATTCGCATCTGGGCGATTTTATCGAGGa > 1:4238216/1‑68 (MQ=255) gagCCAATCTCCATGGAAACGCCGATCGGTGACGACGACGATTCGCATCTGGGCGATTTTATCGAGGa > 1:709266/1‑68 (MQ=255) ccAATCTCCATGGAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAGGa > 1:551090/1‑65 (MQ=255) ccAATCTCCATGGAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAGGa > 1:1928785/1‑65 (MQ=255) aTCTCCATGGAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATc < 1:2501722/57‑1 (MQ=255) tctcCATGGAAACGCCGATCGGTGATGATGAAGATTCGCATCTggggg < 1:606987/48‑1 (MQ=255) tctcCATGGAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGa > 1:1569285/1‑58 (MQ=255) ctcCATGGAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAGGATACCAccc > 1:1045689/1‑68 (MQ=255) ctcCATGGAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAGGATACCACCCTc > 1:1165973/1‑70 (MQ=255) tcCATGGAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTc < 1:940158/51‑1 (MQ=255) tcCATGGAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAg > 1:775836/1‑57 (MQ=255) tcCATGGAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAg > 1:2152072/1‑57 (MQ=255) tcCATGGAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAg > 1:2878610/1‑57 (MQ=255) tcCATGGAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAGGATACCACCCTc > 1:1731627/1‑69 (MQ=255) cATGGAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAGGATACCACCCTc > 1:2465121/1‑67 (MQ=255) cATGGAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAGGATACCACCCTc > 1:1638111/1‑67 (MQ=255) cATGGAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAGGATACCACCCTc > 1:2182538/1‑67 (MQ=255) cATGGAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAGGATACCACCCTCGAGc > 1:687364/1‑71 (MQ=255) aTGGAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCg < 1:3701678/52‑1 (MQ=255) aTGGAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAGGATACCACCCTCGAGCt < 1:345118/71‑1 (MQ=255) aTGGAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAGGATACCACCCTCGAGCt < 1:2922867/71‑1 (MQ=255) ggAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAGGa > 1:3523336/1‑54 (MQ=255) ggAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAGGa > 1:2832599/1‑54 (MQ=255) ggAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAGGa > 1:1751771/1‑54 (MQ=255) ggAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAGGa > 1:2539986/1‑54 (MQ=255) gAAACGCCGATCGGTGATGATGAAGATTCGCATCt > 1:1690168/1‑35 (MQ=255) gAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAGGATACCACCCTCGAGCTGcc > 1:2476499/1‑71 (MQ=255) gAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAGGATACCACCCTCGAGCTGc > 1:1920338/1‑70 (MQ=255) cGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAGGATACCACCCTCGAGCTGCCGCTg < 1:692985/71‑1 (MQ=255) gCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAg < 1:2488736/46‑1 (MQ=255) gCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAGGa > 1:131819/1‑48 (MQ=255) gCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAGGa > 1:2837606/1‑48 (MQ=255) cGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAGGa > 1:2610645/1‑46 (MQ=255) cGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAGGa > 1:187037/1‑46 (MQ=255) cGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAGGATACCACCCTCGAGCTGCCGCTGGAt > 1:807155/1‑71 (MQ=255) tCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGa > 1:3178993/1‑40 (MQ=255) tCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGa > 1:3741131/1‑40 (MQ=255) tCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGa > 1:3348527/1‑40 (MQ=255) tCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGa > 1:3635406/1‑40 (MQ=255) tCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGa > 1:2779735/1‑40 (MQ=255) tCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGa > 1:936429/1‑40 (MQ=255) cGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAGGATACCACCCTCGAGCTGCCGCTGGATTCTg > 1:1543789/1‑71 (MQ=255) cGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAGGATACCACCCTCGAGCTGCCGCTGGATTCTg > 1:4055775/1‑71 (MQ=255) tgatgatgaAGATTCGCATCTGGGGGATTTCATCGAGGATACCACCCTCGAGCTGCCGCTg > 1:4172249/1‑61 (MQ=255) atgatgaAGATTCGCATCTGGGGGATTTCATCGAGGa > 1:1852795/1‑37 (MQ=255) atgatgaAGATTCGCATCTGGGGGATTTCATCGAGGa > 1:3087682/1‑37 (MQ=255) atgatgaAGATTCGCATCTGGGGGATTTCATCGAGGATacc < 1:3098027/41‑1 (MQ=255) atgatgaAGATTCGCATCTGGGGGATTTCATCGAGGATacc < 1:3759643/41‑1 (MQ=255) | AAGACAAGATCCGCAAAGTGCTGAAGATCGCCAAAGAGCCAATCTCCATGGAAACGCCGATCGGTGATGATGAAGATTCGCATCTGGGGGATTTCATCGAGGATACCACCCTCGAGCTGCCGCTGGATTCTG > minE/2065231‑2065362 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |