Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	W3110S.gb	11,430	C→T	7.8%	T119T (ACG→ACA)	yaaI ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	11,430	0	C	T	7.8%	152.0 / 2.7	51	T119T (ACG→ACA)	yaaI	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (19/28);  new base T (2/2);  total (21/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.16e-01

TGTGGCTTCTATATTCTGGCGTTAGTCGTCGCCGATAATTTTCAGCGTGGCCATATCCGATGAGTTCACCGTATGACCCGAAAAGGTGATTTTTGAGACGCAGCGTTTATTGTCGTTATCGCTGTTAATGTTGATCCA  >  W3110S.gb/11361‑11498                                                                      |                                                                     tgtgGCTTCTATATTCTGGCGTTAGTCGTCGCCGATAATTTTCAGCGTGGCCATATCCGATGAGTTCAcc                                                                      <  1:2499501/70‑1 (MQ=255)  gtgGCTTCTATATTCTGGGGTTAGTCGTCGCCGATAATTTTCAGCGTGGCCATATCCGATGAGTTCACCg                                                                     <  1:2971921/70‑1 (MQ=255)  gtgGCTTCTATATTCTGGCGTTAGTCGTCGCCTATAATTTTCAGCGTGGCCATATCCGATGAGTTCACCg                                                                     <  1:309169/70‑1 (MQ=255)  gtgACTTCTATATTCTGGCGTTAGTCGTCGCCGATAATTTTCAGCGTGGCAATATCCGATGAGTTCACCg                                                                     <  1:1309223/70‑1 (MQ=255)   tgGCTTCTATATTCTGGCGTTAGTCGTCGCCGATAATTTTCAGCGTGGCCATATCCGATGAGTTCACCGt                                                                    <  1:3084084/70‑1 (MQ=255)     gCTTCTATATTCTGGCGTTAGTCGTCGCCGATAATTTTCAGCGTGGCCATATCCGATGAGTTCACCGTATg                                                                 <  1:1123598/71‑1 (MQ=255)         cTATATTCTGGCGTTAGTCGTCGCCGATAATTTTCAGCGTGGCCATATCCGATGAGTTCACCGTATGAc                                                               <  1:1270285/69‑1 (MQ=255)         cTATATTCTGGCGTTAGTCGTCGCCGATAATTTTCAGCGTGGCCATATCCGATGAGTTCACCGTATGAc                                                               <  1:1265097/69‑1 (MQ=255)          tataTTCTGGCGTTAGTCGTCGCCGATAATTTTCAGCGTGGCCATATCCGATGAGTTCACCGTATGAccc                                                             >  1:2632818/1‑70 (MQ=255)           atatTCTGGCGTTAGTCGTCGCCGATAATTTTCAGCGTGGCCATATCCGATGAGTTCACTGTATGAccgg                                                            >  1:3744992/1‑68 (MQ=255)           atatTCTGGCGTTAGTCGTCGCCGATAATTTTCAGCGTGGCCATATCCGATGAGTTCACTGTATGAccg                                                             >  1:1845131/1‑68 (MQ=255)              ttCTGGCGTTAGTCGTCGCCGATAATTTTCAGCGTGGCCATATCCGATGAGTTCACCGTATGACCCGaa                                                          >  1:1293408/1‑69 (MQ=255)                cTGGCGTTAGTCGTCGCCGATAATTTTCAGCGTGGCCATATCCGATGAGTTCACCGTATGACCCGAAAAgg                                                      >  1:3346492/1‑71 (MQ=255)                     gTTAGTCGTCGCCGATAATTTTCAGCGTGGCCATATCCGATGAGTTCACCg                                                                     <  1:4147031/51‑1 (MQ=255)                      ttAGTCGTCGCCGATAATTTTCAGCGTGGCCATATCCGATGAGTTCACTGTATGACCGGAAAAGGTGAttt                                                <  1:3306402/71‑1 (MQ=255)                      ttAGTCGTCGCCGATAATTTTCAGCGTGGCCATATCCGATGAGTTCACCGTATGACCCGAAAAGGTGAttt                                                <  1:3200308/71‑1 (MQ=255)                       tAGTCGTCGCCGATAATTTTCAGCGTGGCCATATCCGATGAGTTCACCGTATGACCCGAAAAGGTGAtttt                                               >  1:3024731/1‑71 (MQ=255)                         gtcgtcGCCGATAATTTTCAGCGTGGCCATATCCGATGAGTTCACCGTATGACCCGAAAAGGTGAttttt                                              <  1:4117958/70‑1 (MQ=255)                         gtcgtcGCCGATAATTTTCAGCGTGGCCATATCCGATGAGTTCACCGTATGACCCGAAAAGGTGAttttt                                              <  1:3448364/70‑1 (MQ=255)                          tcgtcgCCGATAATTTTCAGCGTGGCCATATCCGATGAGTTCACCGTATGACCCGaaaa                                                        <  1:1570753/59‑1 (MQ=255)                          tcgtcgCCGATAATTTTCAGCGTGGCCATATCCGATGAGTTCACCGTATGACCCGAAAAGGTGAttttt                                              >  1:3949099/1‑69 (MQ=255)                          tcgtcgCCGATAATTTTCAGCGTGGCCATATCCGATGAGATCACCGTATGACCAGAAAAGGTGAttttt                                              <  1:1866899/69‑1 (MQ=255)                            gtcgCCGATAATTTTCAGCGTGGCCATATCCGATGAGTTCACCGTATGACCCGAAAAGGTGATTTTTgaga                                          >  1:1157145/1‑71 (MQ=255)                                  gATAATTTTCAGCGTGGCCATATCCGATGAGTTCACCGTATGACCCGAAAAGGTGATTTTTGAGACGCAGc                                    >  1:1140658/1‑71 (MQ=255)                                   aTAATTTTCAGCGTGGCCATATCCGATGAGTTCACCGTATGACCCGAAAAGGTGATTTTTGAGACGCAGCg                                   >  1:2920086/1‑71 (MQ=255)                                    tAATTTTCAGCGTGGCCATATCCGATGAGTTCACCGTATGACCCGAAAAGGTGATTTTTGAGACGCAGCGt                                  >  1:1665191/1‑71 (MQ=255)                                       ttttCAGCGTGGCCATATCCGATGAGTTCACCGTATGACCCGAAAAGGTGATTTTTgag                                           >  1:4413234/1‑59 (MQ=255)                                        tttCAGCGTGGCCATATCCGATGAGTTCACCGTATg                                                                 >  1:100804/1‑36 (MQ=255)                                        tttCAGCGTGGCCATATCCGATGAGTTCACCGTATg                                                                 >  1:1804507/1‑36 (MQ=255)                                                  gCCATATCCGATGAGTTCACCGTATGACCCGAAAAGGTGAtttt                                               >  1:3887960/1‑44 (MQ=255)                                                  gCCATATCCGATGAGTTCACCGTATGACCCGAAAAGGTGATTTTTGAGACGCAGCGTTTATTGTCGTTa                      <  1:2452846/69‑1 (MQ=255)                                                   ccATATCCGATGAGTTCACCGTATGACCCGAAAAGGTGATTTTTGAGACGCAGCGTTTATTGTCGTTATCg                   <  1:2159720/71‑1 (MQ=255)                                                     atatCCGATGAGTTCACCGTATGACCCGAAAAGGTGATTTTTGAGACGCAGCGTTTATTGTCGTTATCGc                  >  1:272654/1‑70 (MQ=255)                                                      tatCCGATGAGTTCACTGTATGACCGGAAAAGGTGATTTTTGAGACGCAGCGTTTGTTGTc                          <  1:2072030/61‑1 (MQ=255)                                                      tatCCGATGAGTTCACCGTATGACCCGAACAGGTGATTTTTGAGACGCAGCGTTTATTGTCGtt                       <  1:2571174/64‑1 (MQ=255)                                                      tatCCGATGAGTTCACCGTATGACCCGAAAAGGTGATTTTTGAGACGCAGCGTTTATTGTCGtt                       <  1:2830455/64‑1 (MQ=255)                                                      tatCCGATGAGTTCACCGTATGACCCGAAAAGGTGATTTTTGAGACGCAGCGTTTATTGTCGtt                       <  1:375741/64‑1 (MQ=255)                                                       atCCGATGAGTTCACCGTATGACCCGAAAAGGTGATTTTTGAGACGCAGCGTTTATTGTCGTTATCGCTGt               >  1:2535472/1‑71 (MQ=255)                                                        tCCGATGAGTTCACCGTATGACCCGAAAAGGTGATTTTTGAGACGCAGCGtt                                 >  1:2272718/1‑52 (MQ=255)                                                        tCCGATGAGTTCACCGTATGACCCGAAAAGGTGATTTTTGAGACGCAGCGTTTATTGTCGtt                       <  1:470806/62‑1 (MQ=255)                                                        tCCGATGAGTTCACCGTATGACCCGAAAAGGTGATTTTTGAGACGCAGCGTTTATTGTCGtt                       <  1:1129165/62‑1 (MQ=255)                                                        tCCGATGAGTTCACCGTATGACCCGAAAAGGTGATTTTTGAGACGCAGCGTTTATTGTCGt                        >  1:3853146/1‑61 (MQ=255)                                                               aGTTCACCGTATGACCCGAAAAGGTGATTTTTGAGACGCAGCGTTTATTGTCGTTATCGCTGTTAATGtt        >  1:4101523/1‑70 (MQ=255)                                                               aGTTCACCGTATGACCCGAAAAGGTGATTTTTGAGACGCAGCGTTTATTGTCGTTATCGCTGTTAATGTTg       <  1:1175122/71‑1 (MQ=255)                                                                 ttCACCGTATGACCCGAAAAGGTGATTTTTGAGACGCAGCGTTTATTGTCGTTATCGCTGTTAATGTTg       <  1:911335/69‑1 (MQ=255)                                                                 ttCACCGTATGACCCGAAAAGGTGATTTTTGAGACGCAGCGTTTATTGTCGTTATCGCTGTTAATGTTg       <  1:4393776/69‑1 (MQ=255)                                                                 ttCACCGTATGACCCGAAAAGGTGATTTTTGAGACGCAGCGTTTATTGTCGTTATCGCTGTTAATGTTg       <  1:1084256/69‑1 (MQ=255)                                                                   cACCGTATGACCCGAAAAGGTGATTTTTGAGACGCAGCGTTTAt                              >  1:3961472/1‑44 (MQ=255)                                                                   cACCGTATGACCCGAAAAGGTGATTTTTGAGACGCAGCGTTTATTGTGGTTATCGCTGTTAATGTTGATc    <  1:2257904/70‑1 (MQ=255)                                                                   cACCGTATGACCCGAAAAGGTGATTTTTGAGACGCAGCGTTTATTGTCGTTATCGCTGTTAATGTTGATc    <  1:1134688/70‑1 (MQ=255)                                                                    aCCGTATGACCCGAAAAGGTGATTTTTGAGACGCAGCGTTTATTGTCGTTATCGCTGTTa             <  1:4225393/60‑1 (MQ=255)                                                                    aCCGTATGACCCGAAAAGGTGATTTTTGAGACGCAGCGTTTATTGTCGTTATCGCTGTTAATGTTGATCca  <  1:1911558/71‑1 (MQ=255)                                                                      cGTATGACCCGAAAAGGTGATTTTTGAGACGCAGCGTTTATTGTCGTTATCGCTGTTa             <  1:1823960/58‑1 (MQ=255)                                                                      cGTATGACCCGAAAAGGTGATTTTTGAGACGCAGCGTTTATTGTCGTTATCGCTGTTAATGTTGATcc   <  1:208351/68‑1 (MQ=255)                                                                      |                                                                     TGTGGCTTCTATATTCTGGCGTTAGTCGTCGCCGATAATTTTCAGCGTGGCCATATCCGATGAGTTCACCGTATGACCCGAAAAGGTGATTTTTGAGACGCAGCGTTTATTGTCGTTATCGCTGTTAATGTTGATCCA  >  W3110S.gb/11361‑11498

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑