Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	W3110S.gb	3,936,039	C→T	7.6%	G128G (GGC→GGT)	dppC →	dipeptide transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	3,936,039	0	C	T	7.6%	199.7 / 4.7	66	G128G (GGC→GGT)	dppC	dipeptide transporter
Reads supporting (aligned to +/- strand):  ref base C (28/33);  new base T (2/3);  total (30/36)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.90e-01

CTGTCTGGTAGTTGTGTTATCGCTGATTATGGGCGTTATTCTCGGCCTGATCGCCGGTTACTTTGGCGGCCTGGTCGATAACATCATTATGCGCGTGGTCGATATCATGCTGGCGCTGCCAAGTCTGCTGCTGGCGC  >  W3110S.gb/3935970‑3936106                                                                      |                                                                    cTGTCTGGTAGTTGTGTTATCGCTGATTATGGGCGTTATTCTCGGCCTGATCGCCGGTTACTTTGGCGGcc                                                                    >  1:3714763/1‑71 (MQ=255) cTGTCTGGTAGTTGTGTTATCGCTGATTATGGGCGTTATTCTCGGCCTGATCGCCGGTTACTTTGGCGGcc                                                                    >  1:4119274/1‑71 (MQ=255) cTGTCTGGTAGTTGTGTTATCGCTGATTATGGGCGTTATTCTCGGCCTGATCGCCGGTTACTTTGGCGGcc                                                                    <  1:219355/71‑1 (MQ=255) cTGTCTGGCAGTTGTGTTATCGCTGATTATGGGCGTTATTCTCGGCCTGATCGCCGGTTACTTTGGCGGcc                                                                    <  1:1343121/71‑1 (MQ=255)      tGGTAGTGGTGTTATCGCTGATTATGGGCGTTATTCTCGGCCTGATCGCTGGTTACTTTGGCGGTCTGGTc                                                               >  1:3046853/1‑71 (MQ=255)      tGGTAGTGGTGTTATCGCTGATTATGGGCGTTATTCTCGGCCTGATCGCTGGTTACTTTGGCGGTATGGTc                                                               >  1:347243/1‑71 (MQ=255)        gTAGTTGTGTTATCGCTGATTATGGGCGTTATTCTCGGCCTGATCGCCGGTTACTTTGGCGGCCTGGTc                                                               <  1:4015792/69‑1 (MQ=255)         tAGTTGTGTTATCGCTGATTATGGGCGTTATTCTCGGCCTGATCGCCGGTTACTTTGGCGGCCTGGTCGAt                                                            <  1:975527/71‑1 (MQ=255)         tAGTTGTGTTATCGCTGATTATGGGCGTTATTCTCGGCCTGATCGCCGGTTACTTTGGCGGCCTGGTCGAt                                                            <  1:1155208/71‑1 (MQ=255)              gtgtTATCGCTGATTATGGGCGTTATTCTCGGCCTGATCGCCGGTTACTTTGGCTGCCTGGTCGATAAcat                                                       >  1:1004994/1‑71 (MQ=255)              gtgtTATCGCTGATTATGGGCGTTATTCTCGGCCTGATCGCCGGTTACTTTGGCGGCCTGGTCGATAAcat                                                       >  1:1140026/1‑71 (MQ=255)              gtgtTATCGCTGATTATGGGCGTTATTCTCGGCCTGATCGCCGGTTACTTTGGCGGCCTGGTCGATAAcat                                                       >  1:3680895/1‑71 (MQ=255)              gtgtTATCGCTGATTATGGGCGTTATTCTCGGCCTGATCGCCGGTTACTTTGGCGGCCTGGTCGATAAca                                                        >  1:667959/1‑70 (MQ=255)                 ttATCGCTGATTATGGGCGTTATTCTCGGCCTGATCGCCGGTTACTTTGGCGGCCTGGTCGATAAcatcat                                                    <  1:3340169/71‑1 (MQ=255)                 ttATCGCTGATTATGGGCGTTATTCTCGGCCTGATCGCCGGTTACTTTGGCGGCCTGGTCGATAAcatca                                                     <  1:1748549/70‑1 (MQ=255)                  tATCGCTGATTATGGGCCTTATTCTCGGCCTGATCGCCGGTTACTTTGGCGGCCTg                                                                  >  1:1181761/1‑56 (MQ=255)                   aTCGCTGATTATGGGCGTTATTCTCGGCCTGATCGCCGGTTACTTTGGCGGCCTGGTCGATAAcatca                                                     <  1:2250318/68‑1 (MQ=255)                   aTCGCTGATTATGGGCGTTATTCTCGGCCTGATCGCCGGTTACTTTGGCGGCCTGGTCGATAACATCATTa                                                  >  1:3839948/1‑71 (MQ=255)                   aTCGCTGATTATGGGCGTTATTCTCGGCCTGATCGCCGGTTACTTTGGCGGCCTGGTCGATAACATCATTa                                                  >  1:2256703/1‑71 (MQ=255)                    tCGCTGATTATGGGCGTTATTCTCGGCCTGATCGCCGGTTACTTTGGCGGCCTGGTCGATAACATCATTa                                                  <  1:1760153/70‑1 (MQ=255)                    tCGCTGATTATGGGCGTTATTCTCGGCCTGATCGCCGGTTACTTTGGCGGCCTGGTCGATAACATCATTa                                                  <  1:487658/70‑1 (MQ=255)                     cGCTGATTATGGGCGTTATTCTCGGCCTGATCGCCGGTTACTTTGGCGGCCTGGTCGATAACATCATTATg                                                >  1:2734890/1‑71 (MQ=255)                      gCTGATTATGGGCGTTATTCTCGGCCTGATCGCCGGTTACTTTGGCGGCCTGGTCGATAAc                                                         >  1:4195162/1‑61 (MQ=255)                      gCTGATTATGGGCGTTATTCTCGGCCTGATCGCCGGTTACTTTGGCGGCCTGGTCGATAACATCATTATgc                                               <  1:3547085/71‑1 (MQ=255)                       cTGATTATGGGCGTTATTCTCGGCCTGATCGCCGGTTACTTTGGCGGCCTGGTCGATAACATCATTATgcg                                              >  1:2518460/1‑71 (MQ=255)                          aTTATGGGCGTTATTCTCGGCCTGATCGCCGGTTACTTTGGCGGCCTGGTCGATAACATCATTATgcgc                                             <  1:1564192/69‑1 (MQ=255)                          aTTATGGGCGTTATTCTCGGCCTGATCGCCGGTTACTTTGGCGGCCTGGTCGATAACATCATTATgcgc                                             <  1:3590858/69‑1 (MQ=255)                          aTTATGGGCGTTATTCTCGGCCTGATCGCCGGTTACTTTGGCGGCCTGGTCGATAACATCATTATGcgcg                                            <  1:3337973/70‑1 (MQ=255)                           ttATGGGCGTTATTCTCGGCCTGATCGCTTGTTACTTTGGCGGTCTg                                                                  <  1:730774/47‑1 (MQ=255)                           ttATGGGCGTTATTCTCGGCCTGATCGCTGGTTACTTTGGCGGTCTg                                                                  <  1:2722313/47‑1 (MQ=255)                           ttATGGGCGTTATTCTCGGCCTGATCGCCGGTTACTTTGGCGGCCTGGTCGATAACATCATTATgc                                               >  1:3266049/1‑66 (MQ=255)                           ttATGGGCGTTATTCTCGGCCTGATCGCCGGTTACTTTGGCGGCCTGGTCGATAACATCATTATgc                                               >  1:2552127/1‑66 (MQ=255)                             aTGGGCGTTATTCTCGGCCTGATCGCCGGTTACTTTGGCGGCCTGGTCGATAACATCATTATGCGCGTGGt                                        >  1:3518040/1‑71 (MQ=255)                             aTGGGCGTTATTCTCGGCCTGATCGCCGGTTACTTTGGCGGCCTGGTCGATAACATCATTATGCGCGTGGt                                        >  1:1442336/1‑71 (MQ=255)                              tGGGCGTTATTCTCGGCCTGATCGCCGGTTACTTTGGCGGCCTGGTCGATAACATCATTATGCGCGTGGTc                                       <  1:3945620/71‑1 (MQ=255)                                ggCGTTATTCTCGGCCTGATCGCTGGTTACTTTGGCGGTCTGGTCGATAACATCATCATgc                                               <  1:368217/61‑1 (MQ=255)                                 gCGTTATTCTCGGCCTGATCGCCGGTTACTTTGGCGGCCTGGTCGATAACATCATTATGCGCGTGGTCGa                                     <  1:1034600/70‑1 (MQ=255)                                  cGTTATTCTTGGCCTGATCGCCGGTTACTTTGGCGGCCTGGTc                                                               <  1:2743555/43‑1 (MQ=255)                                    ttATTCTCGGCCTGATCGCCGGTTACTTTggcggc                                                                     <  1:273873/35‑1 (MQ=255)                                      aTTCTCGGCCTGATCGCCGGTTACTTTGGCGGCCTGGTCGATAACATCATTATGCGCGTgg                                         >  1:1013225/1‑61 (MQ=255)                                      aTTCTCGGCCTGATCGCCGGTTACTTTGGCGGCCTGGTCGATAACATCATTATGCGCGTGGTCGATATCAt                               >  1:2882654/1‑71 (MQ=255)                                      aTTCTCGGCCTGATCGCCGGTTACTTTGGCGGCCTGGTCGATAACATCATTATGCGCGTGGTCGATATCAt                               >  1:4224803/1‑71 (MQ=255)                                      aTTCTCGGCCTGATCGCCGGTTACTTTGGCGGCCTGGTCGATAACATCATTATGCGCGTGGTCGATATCAt                               >  1:3636888/1‑71 (MQ=255)                                        tctcGGCCTGATCGCCGCTTACTTTGGCGGCCTGGTCGATAACATCATTATGCGCGTGGTCGATATCATGc                             >  1:1541398/1‑71 (MQ=255)                                         ctcGGCCTGATCGCCGGTTACTTTGGCGGCCTGGTCGATAACATCATTATGCGCGTGGTCGATATCATGc                             <  1:3579525/70‑1 (MQ=255)                                         ctcGGCCTGATCGCCGGTTACTTTGGCGGCCTGGTCGATAACATCATTATGCGCGTGGTCGATATCATGc                             <  1:2061343/70‑1 (MQ=255)                                         ctcGGCCTGATCGCCGGTTACTTTGGCGGCCTGGTCGATAACATCATTATGCGCGTGGTCGATATCATGc                             <  1:3709709/70‑1 (MQ=255)                                         ctcGGCCTGATCGCCGGTTACTTTGGCGGCCTGGTCGATAACATCATTATGCGCGTGGTCGATATCATGCt                            >  1:4014336/1‑71 (MQ=255)                                          tcGGCCTGATCGCCGGTTACTTTGGCGGCCTGGTCGATAACATCATTATGCGCGTGGTCGATATCATGCTg                           >  1:3467299/1‑71 (MQ=255)                                            ggCCTGATCGCCGGTTACTTTGGCGGCCTGGTCGATAACATCATTATGCGCGTGGTCGATATCATGCTgg                          <  1:457439/70‑1 (MQ=255)                                             gCCTGATCGCCGGTTACTTTGGCGGCCTGGTCGATAACATCATTATGCTCGTGGTCGATATCATGCTGgcg                        <  1:569004/71‑1 (MQ=255)                                                  aTCGCCGGTTACTTTGGCGGCCTGGTCGATAACATCATTATGCGCGTGGTCTATATCATGCTGGCGCTGcc                   <  1:114067/71‑1 (MQ=255)                                                   tCGCCGGTTACTTTGGCGGCCTGGTCGATAACATCATTATGCGCGTGGTCGATATCATGCTGGCGCTGcc                   >  1:3098068/1‑70 (MQ=255)                                                   tCGCCGGTTACTTTGGCGGCCTGGTCGATAACATCATTATGCGCGTGGTCGATATCATGCTGGCGCTGcc                   >  1:1228909/1‑70 (MQ=255)                                                     gCCGGTTACTTTGGCGGCCTGGTCGATAACATCATTATGCGCGTGGTCGATATCATGCTGGCGCTGCCAAg                <  1:597767/71‑1 (MQ=255)                                                        ggTTACTTTGGCGGCCTGGTCGATAACATCATTATGCGCGTGGTCGATATCATGCTGGCGCTGCCAAGTct             >  1:1013616/1‑71 (MQ=255)                                                         gTTACTTTGGCGGCCTGGTCGATAACATCATTATGCGCGTGGTCGa                                     <  1:3303091/46‑1 (MQ=255)                                                         gTTACTTTGGCGGCCTGGTCGATAACATCATTATGCGCGTGGTCGATATCATGCt                            <  1:2895702/55‑1 (MQ=255)                                                         gTTACTTTGGCGGCCTGGTCGATAACATCATTATGCGCGTGGTCGATATCATGCt                            <  1:443581/55‑1 (MQ=255)                                                         gTTACTTTGGCGGCCTGGTCGATAACATCATTATGCGCGTGGTCGATATCATGCTGGCGCTGCCAAGTctg            >  1:1178111/1‑71 (MQ=255)                                                          ttACTTTGGCGGCCTGGTCGATAACATCATTATGCGCGTGGTCGATATCa                                <  1:1112222/50‑1 (MQ=255)                                                          ttACTTTGGCGGCCTGGTCGATAACATCATTATGCGCGTGGTCGATATCATGCTGGCGCTGCCAAGTctgc           <  1:1713914/71‑1 (MQ=255)                                                          ttACTTTGGCGGCCTGGTCGATAACATCATTATGCGCGTGGTCGATATCATGCTGGCGCTGCCAAGTctgc           <  1:2934607/71‑1 (MQ=255)                                                           tACTTTGGCGGCCTGGTCGATAACATCATTATGCGCGTGGTCGATATCATGCTGGCGCTGCCAAGTctgc           >  1:4288627/1‑70 (MQ=255)                                                            aCTTTGGCGGCCTGGTCGATAACATCATTATGCGCGTGGTCGATATCATGCTGGCGCTGCCAAGTctgctg         >  1:3927419/1‑71 (MQ=255)                                                            aCTTTGGCGGCCTGGTCGATAACATCATTATGCGCGTGGTCGATATCATGCTGGCGCTGCCAAGTctgct          <  1:2257480/70‑1 (MQ=255)                                                             cTTTGGCGGCCTGGTCGATAACATCATTATGCGCGTGGTCGATATCATGCTGGCGCTGCCAAGTctgctgc        <  1:1501718/71‑1 (MQ=255)                                                              tttGGCGGCCTGGTCGATAACATCATTATGCGCGt                                           <  1:2247681/35‑1 (MQ=255)                                                              tttGGCGGCCTGGTCGATAACATCATTATGCGCGTGGTCGa                                     <  1:4181587/41‑1 (MQ=255)                                                                tGGCGGCCTGGTCGATAACATCATTATGCGCGTGGTCGATATCATGCTGGCGCTGCCAAGTctgctgctg      <  1:1546871/70‑1 (MQ=255)                                                                tGGCGGCCTGGTCGATAACATCATTATGCGCGTGGTCGATATCATGCTGGCGCTGCCAAGTCTGCTGCTgg     >  1:1258791/1‑71 (MQ=255)                                                                 ggcggcCTGGTCGATAACATCATTATGCGCGTGGTCGATATCATGCTGGCGCTGCCAAGTCTGCTGCTGgc    >  1:1871090/1‑71 (MQ=255)                                                                   cggcCTGGTCGATAACATCATTATGCGCGTGGTCGATATCATGCTGGCGCTGCCAAGTCTGCTGCTGgcgc  <  1:4393669/71‑1 (MQ=255)                                                                      |                                                                    CTGTCTGGTAGTTGTGTTATCGCTGATTATGGGCGTTATTCTCGGCCTGATCGCCGGTTACTTTGGCGGCCTGGTCGATAACATCATTATGCGCGTGGTCGATATCATGCTGGCGCTGCCAAGTCTGCTGCTGGCGC  >  W3110S.gb/3935970‑3936106

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑