| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 4,013,464 | C→T | 6.3% | Y862Y (TAC→TAT) | rbbA → | fused ribosome‑associated ATPases |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 4,013,464 | 0 | C | T | 6.3% | 185.9 / 2.6 | 63 | Y862Y (TAC→TAT) | rbbA | fused ribosome‑associated ATPases |
| Reads supporting (aligned to +/- strand): ref base C (27/31); new base T (2/2); total (29/34) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.91e-01 | |||||||||||
CAGTTTTCCGGGATGATCGATCCGGTAGCTTCGCTGGAAGGGCCTGGACGTTGGATCGGCGAGGTTTACCCGACCAGTCATTTTCTGACTATCGCCCGCGGGACGTTCTCGAAAGCGCTGGATCTGACTGATTTGTGG > W3110S.gb/4013396‑4013533 | caGTTTTCCGGGATGATCGATCCGGTAGCTTCGCTGGAAGGGCCTGGACGTTGGATCGGCGAGGTTTAccc < 1:1735508/71‑1 (MQ=255) aGTTTTCCGGGATGATCGATCCGGTAGCTTCGCTGGAAGGGCCTGGACGTTGGATCGGCGAGGTTTAccc > 1:2007395/1‑70 (MQ=255) aGTTTTCCGGGATGATCGATCCGGTAGCTTCGCTGGAAGGGCCTGGACGTTGGATCGGCGAGGTTTAccc > 1:1703884/1‑70 (MQ=255) ttttCCGGGATGATCGATCCGGTAGCTTCGCTGGAAGGGCCTGGACGTTGGATCGGCGAGGTTTAccc < 1:2309850/68‑1 (MQ=255) gggATGATCGATCCGGTAGCTTCGCTGGAAGGGCCTGGACGTTGGATCGGCGAGGTTTACCCGACCAGTCa > 1:3897539/1‑71 (MQ=255) gggATGATCGATCCGGTAGCTTCGCTGGAAGGGCCTGGACGTTGGATCGGAGAGGTTTACCCGACCAGTCa > 1:2378547/1‑71 (MQ=255) gatgatCGATCCGGTAGCTTCGCTGGAAGGGCCTGGACGTTGGATCGGCGAGGTTTACCCGACCAGTCAtt < 1:3730389/71‑1 (MQ=255) tgatCGATCCGGTAGCTTCGCTGGAAGGGCCTGGACGTTGGATCGGCGAGGTTTACCCGACCAGTCAtttt < 1:1391201/71‑1 (MQ=255) atcgatcCGGTAGCTTCGCTGGAAGGGCCTGGACGTTGGATCGGCGAGGTTTACCCGACCAGTCATTTTCt < 1:1804913/71‑1 (MQ=255) atcgatcCGGTAGCTTCGCTGGAAGGGCCTGGACGTTGGATCGGCGAGGTTTACCCGACCAGTCATTTTCt < 1:4206410/71‑1 (MQ=255) atcgatcCGGTAGCTTCGCTGGAAGGGCCTGGACGTTGGATCGGCGAGGTTTACCCGACCAGTCATTTTCt < 1:2655559/71‑1 (MQ=255) atcgatcCGGTAGCTTCGCTGGAAGGGCCTGGACGTTGGATCGGCGAGGTTTACCCGACCAGTCATTTTCt < 1:2728849/71‑1 (MQ=255) cgatcCTGTAGCTTCGCTGGAAGGGCCTGGACGTTGGATCGGCGAGGTTTAc > 1:2716718/1‑52 (MQ=255) cgatcCGGTAGCTTCGCTGGAAGGGCCTGGACGTTGGATCGGCGAGGTTTACCCGACCAGTCATTTTCTGa > 1:309025/1‑71 (MQ=255) gatcCGGTAGCTTCGCTGGAAGGGCCTGGACGTTGGATCGGCGAGGTTTACCCGACCAGTCATTTTCt > 1:3670956/1‑68 (MQ=255) gatcCGGTAGCTTCGCTGGAAGGGCCTGGACGTTGGATCGGCGAGGTTTACCCGACCAGTCATTTTCTg < 1:2881709/69‑1 (MQ=255) gatcCGGTAGCTTCGCTGGAAGGGCCTGGACGTTGGATCGGCGAGGTTTACCCGACCAGTCATTTTCTGa > 1:3934187/1‑70 (MQ=255) gatcCGGTAGCTTCGCTGGAAGGGCCTGGACGTTGGATCGGCGAGGTTTACCCGACCAGTCATTTTCTGAc < 1:941940/71‑1 (MQ=255) atcCGGTAGCTTCGCTGGAAGGGCCTGGACGTTGGATCGGCGAGGTTTAGCCGACCAGt < 1:1539634/59‑1 (MQ=255) atcCGGTAGCTTCGCTGGAAGGGCCTGGACGTTGGATCGGCGAGGTTTACCCGACCAGt < 1:4202664/59‑1 (MQ=255) ccGGTAGCTTCGCTGGAAGGGCCTGGACGTTGGATCGGCGAGGTTTAc < 1:2946750/48‑1 (MQ=255) ccGGTAGCTTCGCTGGAAGGGCCTGGACGTTGGATCGGCGAGGTTTACCCGACCAGTCAttt > 1:42885/1‑62 (MQ=255) gTAGCTTCGCTGGAAGGGCCTGGACGTTGGATCGGCGAGGTTTACCCGACCAGTCATTTTCTGAc < 1:2499654/65‑1 (MQ=255) gTAGCTTCGCTGGAAGGGCCTGGACGTTGGATCGGCGAGGTTTACCCGACCAGTCATTTTCTGACTATCGc > 1:1262626/1‑71 (MQ=255) tAGCTTCGCTGGAAGGGCCTGGACGTTGGATCGGCGAGGTTTAccc < 1:3494505/46‑1 (MQ=255) aGCTTCGCTGGAAGGGCCTGGACGTTGGATCGGCGAGGTTTACCCGACCAGTCATTTTCTGACTATCGccc > 1:1012256/1‑71 (MQ=255) cTTCGCTGGAAGGGCCTGGACGTTGGATCGGCGAGGTTTACCCGACCAGTCATTTTCTGACTATCGCCcgc < 1:2200614/71‑1 (MQ=255) tCGCTGGAAGGGCCTGGACGTTGGATCGGCGAGGTTTACCCGACCAGTCATTTTCTGACTATCGCCCGCgg > 1:3958107/1‑71 (MQ=255) tCGCTGGAAGGGCCTGGACGTTGGATCGGCGAGGTTTACCCGACCAGTCATTTTCTGACTATCGCCCGCgg > 1:1350709/1‑71 (MQ=255) gCTGGAAGGGCCTGGACGTTGGATCGGCGAGGTTTACCCGACCAGTCATTTTCTGACTATCGCCCGCggg < 1:210551/70‑1 (MQ=255) gCTGGAAGGGCCTGGACGTTGGATCGGCGAGGTTTACCCGACCAGTCATTTTCTGACTATCGCCCGCggg < 1:6636/70‑1 (MQ=255) cTGGAAGGGCCTGGACGTTGGATCGGCGAGGTTTACCCGACCAGTCATTTTCTGACTATCGCCCGCGGGAc < 1:3752025/71‑1 (MQ=255) aaGGGCCTGGACGTTGGATCGGCGAGGTTTACCCGACCAGTCATTTTCTGACTATCGCCCGCGGGACGTtc > 1:4012365/1‑71 (MQ=255) gCCTGGACGTTGGGTCGGCGAGGTTTACCCGACCAGTCAttt < 1:4095361/42‑1 (MQ=255) gCCTGGACGTTGGATCGGCGAGGTTTACCCGACCAGTCATTTTCTGACTATCGCCCGCGGGACGTTCTCGa < 1:149036/71‑1 (MQ=255) gCCTGGACGTTGGATCGGCGAGGTTTACCCGACCAGTCATTTTCTGACTATCGCCCGCGGGACGTTCTCGa < 1:3978224/71‑1 (MQ=255) ccTGGACGTTGGATCGGCGAGGTTTACCCGACCAGTCATTTTCTGACTATCGCCCGCGGGACGTTCTCGa < 1:3118133/70‑1 (MQ=255) caggACGTTGGATCGGCGAGGTTTATCCCACCAGTCATTTTCTGACt < 1:2948337/45‑1 (MQ=255) caggACGTTGGATCGGCGAGGTTTATCCCACCAGTCATTTTCTGACt < 1:209985/45‑1 (MQ=255) cTGGACGTTGGATCGGCGAGGTTTACCCGACCAGTCATTTTc > 1:146837/1‑42 (MQ=255) ggACGTTGGATCGGCGAGGTTTACCCGACCAGTCATTTTCTGAc > 1:1041898/1‑44 (MQ=255) gACGTTGGATCGGCGAGGTTTACCCGACCAGTCATTTTCTGACTATCGCCcgc < 1:2118111/53‑1 (MQ=255) gACGTTGGATCGGCGAGGTTTACCCGACCAGTCATTTTCTGACTATCGCCCGCGGGACGTTCTCGAAAgcg > 1:3575646/1‑71 (MQ=255) gACGTTGGATCGGCGAGGTTTACCCGACCAGTCATTTTCTGACTATCGCCCGCGGGACGTTCTCGAAAgc > 1:1130436/1‑70 (MQ=255) gACGTTGGATCGGCGAGGTTTACCCGACCAGTCATTTTCTGACTATCGCCCGCGGGACGTTCTCGAAAgc > 1:2533428/1‑70 (MQ=255) ggATCGGCGAGGTTTACCCGACCAGTCATTTTCTGACTATCGCCCGCGGGACGTTCTCGAAAGCGCTGGAt > 1:3970337/1‑71 (MQ=255) aTCGGCGAGGTTTACCCGACCAGTCATTTTCTGACTATCGCCCGCGGGACGTTCTCGAAAgcgc < 1:333709/64‑1 (MQ=255) aTCGGCGAGGTTTACCCGACCAGTCATTTTCTGACTATCGCCCGCGGGACGTTCTCGAAAGCGCTGGATct < 1:3449662/71‑1 (MQ=255) aTCGGCGAGGTTTACCCGACCAGTCATTTTCTGACTATCGCCCGCGGGACGTTCTCGAAAGCGCTGGATct < 1:4164273/71‑1 (MQ=255) tCGGCGAGGTTTACCCGACCAGTCATTTTCTGACTATCGCCCGCGGGACGTTCTCGAAAGCGCTGGATct > 1:3007188/1‑70 (MQ=255) cagCGAGGTTTATCCCACCAGTCATTTTCTGACTATCGCCCGCGGAACGTTCTCGAAAGCGCTGGATctg > 1:2981410/3‑70 (MQ=255) cGGCGAGGTTTATCC‑‑CAAGTCATTTTCTGACTATCGCCCGCGGAACGtt > 1:3843949/1‑49 (MQ=255) cGGCGAGGTTTACCCGACCAGTCATTTTCTGAATATCGCCCGCGGGACGTTCTCGAAAGCGCTGGATctga > 1:3034781/1‑71 (MQ=255) ggCGAGGTTTACCCGACCAGTCATTTTCTGACTATCGCCCGCGGGACGTTCTCGAAAGCGCTGGAt > 1:3699213/1‑66 (MQ=255) gCGAGGTTTACCCGACCAGTCATTTTCTGACTATCGCCCGCGGGACGTTCTCGAAAGCGCTGGATctgact < 1:2081280/71‑1 (MQ=255) gCGAGGTTTACCCGACCAGTCATTTTCTGACTATCGCCCGCGGGACGTTCTCGAAAGCGCTGGATctgact < 1:3302798/71‑1 (MQ=255) cGAGGTTTACCCGACCAGTCATTTTCTGACTATCGCCCGCGGGACGTTCTCGAAAGCGCTGGATctgactg < 1:1741863/71‑1 (MQ=255) cGAGGTTTACCCGACCAGTCATTTTCTGACTATCGCCCGCGGGACGTTCTCGAAAGCGCTGGATct < 1:627245/66‑1 (MQ=255) aGGTTTACCCGACCAGTCATTTTCTGACTATCGCCCGCGGGACGTTCTCGaa > 1:1403924/1‑52 (MQ=255) ggTTTACCCGACCAGTCATTTTCTGACTATCGCCCGCGGGACGTTCTCGAAAGCGCTGGATCTGACTGAtt < 1:3600637/71‑1 (MQ=255) gTTTACCCGACCAGTCATTTTCTGACTATCGCCCGCGGGACGTTCTCGa < 1:2314379/49‑1 (MQ=255) tttACCCGACCAGTCATTTTCTGACTATCGCCCGCGGGACGTTCTCGAAAGCGCTGGATCTGACTGATTtg < 1:1707007/71‑1 (MQ=255) ttACCCGACCAGTCATTTTCTGACTATCGCCCGCgg > 1:1742717/1‑36 (MQ=255) ttACCCGACCAGTCATTTTCTGACTATCGCCCGCgg > 1:3710757/1‑36 (MQ=255) ttACCCGACCAGTCATTTTCTGACTATCGCCCGCTGGACGTTCTCGAAAGCGCTGGATCTGACTGATTtg < 1:3013967/70‑1 (MQ=255) ttACCCGACCAGTCATTTTCTGACTATCGCCCGCGGGACGTTCTCGAAAGCGCTGGATCTGACTGATTtgt > 1:725413/1‑71 (MQ=255) tACGCGACCAGTCATTTTCTGACTATCGCCCGCGGGACGTTCTCGAAAGCGCTGGATCTGACTGATTtgt < 1:2078184/70‑1 (MQ=255) tACCCGACCAGTCATTTTCTGACTATCGCCCGCGGGACGTTCTCGaaa > 1:1527973/1‑48 (MQ=255) tACCCGACCAGTCATTTTCTGACTATCGCCAGCGGGACGTTCTCGAAAGCGCTGGATCTGACTGATTtgtg > 1:3511525/1‑71 (MQ=255) aCCCGACCAGTCATTTTCTGACTATCGCCCGCGGGACGTTCTCGAAAGCGCTGGATCTGACTGATTTGTgg > 1:1397864/1‑71 (MQ=255) | CAGTTTTCCGGGATGATCGATCCGGTAGCTTCGCTGGAAGGGCCTGGACGTTGGATCGGCGAGGTTTACCCGACCAGTCATTTTCTGACTATCGCCCGCGGGACGTTCTCGAAAGCGCTGGATCTGACTGATTTGTGG > W3110S.gb/4013396‑4013533 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |