| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 651,883 | G→T | 10.8% | M142I (ATG→ATT) | citA → | sensory histidine kinase in two‑component regulatory system with citB |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 651,883 | 0 | G | T | 10.8% | 166.2 / 4.4 | 55 | M142I (ATG→ATT) | citA | sensory histidine kinase in two‑component regulatory system with citB |
| Reads supporting (aligned to +/- strand): ref base G (21/28); new base T (2/4); total (23/32) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TGCAGTTCACCAAACAGGGCGCGCTGGAGAAAGGGGAGAGCTACTTCATTACCGGGAAAGGGTCAATGGGGATGGCGATGCGCGCCAAAACGCCAATCTTTGATGACGATGGAAAAGTCATCGGCGTGGTGTCGATT > W3110S.gb/651816‑651952 | tGCAGTTCACCAAACAGGGCGCGCTGGAGAAAGGGGAGAGCTACTTCATTACCGGGAAAGGGTCAATgg < 1:1697490/69‑1 (MQ=255)tGCAGTTCACCAAACAGGGCGCGCTGGAGAAAGGGGAGAGCTACTTCATTACCGGGAAAGGGTCAATgg < 1:1560769/69‑1 (MQ=255) cAGTTCACCAAACAGGGCGCGCTGGAGAAAGGGGAGAGCTACTTCATTACCGGGAAAGGGTCAATGGGGa < 1:532320/70‑1 (MQ=255) cAGTTCACCAAACAGGGCGCGCTGGAGAAAGGGGAGAGCTACTTCATTACCGGGAAAGGGTCAATGGGGAt > 1:59396/1‑71 (MQ=255) cACCAAACAGGGCGCGCTGGAGAAAGGGGAGAGCTACTTCATTACCGGGAAAGGGTCAATGGGGATGGCg < 1:3936498/70‑1 (MQ=255) ccAAACAGGGCGCGCTGGAGAAAGGGGAGAGCTACTTCATTACCGGGAAAGGGTCAATGGGGATGGCGATg > 1:4327820/1‑71 (MQ=255) aaaCAGGGCGCGCTGGAGAAAGGGGAGAGCTACTTCATTACCGGGAAAGGGTCAATGGGGATGGCGATgcg > 1:2594893/1‑71 (MQ=255) aaCAGGGCGCGCTGGAGAAAGGGGAGAGCTACTTCATTACCGGGAAAGGGTCAATGGGGATGGCGATgcgc > 1:4143098/1‑71 (MQ=255) cTGGAGAAAGGGGAGAGCTACTTCATTACCTGGAAAGGGTCAATGGGGATGGc < 1:298342/53‑1 (MQ=255) tGGAGAATGGGGAGAGCTTCTTCATTACCGGGAAAGGGTCAATGGGGATGGc < 1:3869464/52‑1 (MQ=39) ggAGAAAGGGGAGAGCTACTTCATTACCGGGAAAGGGTCAATGGGGATGGCGATGCGCGCCaa < 1:4278288/63‑1 (MQ=255) ggAGAAAGGGGAGAGCTACTTCATTACCGGGAAAGGGTCAATGGGGATGGCGATGCGCGCCaa < 1:1158716/63‑1 (MQ=255) gagaAAGGGGAGAGCTACTTCATTACCGGGAAAGGGTCAATGGGGATGGCGATGCGCGCCAAAACGCCaa > 1:2677812/1‑70 (MQ=255) gagaAAGGGGAGAGCTACTTCATTACCGGGAAAGGGTCAATGGGGATGGCGATGCGCGCCAAAACGCCa < 1:2613115/69‑1 (MQ=255) gagaAAGGGGAGAGCTACTTCATTACCGGGAAAGGGTCAATGGGGATGGCGATGCGCGCCAAAACGCCAAt > 1:1392204/1‑71 (MQ=255) aaGGGGAGAGCTACTTCATTACCGGGAAAGGGTCAATGGGGATGGCGATGCGCGCCAAAACGCCAATCttt < 1:1929513/71‑1 (MQ=255) aGGGGAGAGCTACTTCATTACCGGGAAAGGGTCAATg > 1:3468288/1‑37 (MQ=255) gagaGCTACTTCATTACCGGGAAAGGGTCAATGGGGATGGCGATGCGCGCCAAAACGCCAATCTTtgatga > 1:3591208/1‑71 (MQ=255) gagaGCTACTTCATTACCGGGAAAGGGTCAATGGGGATGGCGATGCGCGCCAAAACGCCAATCTTtgatga < 1:1646040/71‑1 (MQ=255) gagaGCTACTTCATTACCGGGAAAGGGTCAATGGGGATGGCGATGCGCGCCAAAACGCCAATCTTtgatga > 1:1359440/1‑71 (MQ=255) agagCTACTTCATTACCGGGAAAGGGTCAATGGGGATGGCGATGCGCGCCAAAACGCCAATCTTTGATGAc < 1:1865159/71‑1 (MQ=255) agagCTACTTCATTACCGGGAAAGGGTCAATGGGGATGGCGATGCGCGCCAAAACGCCAATCTTTGATGAc < 1:3328053/71‑1 (MQ=255) cTACTTCATTACCGGGAAAGGGTCAATGGGGATGGCGa > 1:2893388/1‑38 (MQ=255) tACTTCATTACCGGGAAACGGTCAATGGGGATGGc < 1:268302/35‑1 (MQ=255) aCTTCATTACCGGGAAAGGGTCAATGGGGATGGCGATGCGCGCCAAAACGCCAATCTTtg > 1:2765414/1‑60 (MQ=255) cTTCATTACCGGGAAAGGGTCAATGGGGATGGCGATGCGCGCCAAAACGCCAATCttt < 1:1817628/58‑1 (MQ=255) cTTCATTACCGGGAAAGGGTCAATGGGGATGGCGATGCGCGCCAAAACGCCAATCTTTGATGACGATgg < 1:1153923/69‑1 (MQ=255) cTTCATTACCGGGAAAGGGTCAATGGGGATGGCGATGCGCGCCAAAACGCCAATCTTTGATGACGATGGaa < 1:1464835/71‑1 (MQ=255) cTTCATTACCGGGAAAGGGTCAATGGGGATGGCGATGCGCGCCAAAACGCCAATCTTTGATGACGATGGaa < 1:1530816/71‑1 (MQ=255) ttCATTACCGGGAAAGGGTCAATGGGGATGGCGATGCGCGCCaa < 1:2279313/44‑1 (MQ=255) cATTACCGGGAAAGGGTCAATGGGGATGGCGATGCGCGCCAAAACGCCAATCTTtgat > 1:2582243/1‑58 (MQ=255) cATTACCGGGAAAGGGTCAATGGGGATGGCGATGCGCGCCAAAACGCCAATCTTTGATGACGATGGAAAAg > 1:2909059/1‑71 (MQ=255) aTTACCGGGAAAGGGTCAATGGGGATGGCGATGCGCGCCAAAACGCCAATCtt > 1:2502901/1‑53 (MQ=255) tACCGGGAAAGGGTCAATGGGGATGGCGATGCGCGCCAAAACGCCAATCTTTGATGACGATGGAAAAGTCa > 1:894166/1‑71 (MQ=255) aCCGGGAAAGTGTCAATGGGGATGGCGATGCGCGCCAAAACGCCAATCTTTGATGACGATGGAAAAGTCAt > 1:440737/1‑71 (MQ=255) aCCGGGAAAGGGTCAATGGGGATGGCGATGCGCGCCAAAACGCCAATCTTtga > 1:2353999/1‑53 (MQ=255) cGGGAAAGGGTCAATTGGCATGGCGATGCGTGCCAAAACGCCAATCTTtga < 1:2429994/51‑1 (MQ=255) cGGGAAAGGGTCAATTGGCATGGCGATGCGTGCCAAAACGCCAATCTTtga < 1:613953/51‑1 (MQ=255) cGGGAAAGGGTCAATTGGCATGGCGATGCGTGCCAAAACGCCAATCTTtga < 1:474005/51‑1 (MQ=255) cGGGAAAGGGTCAATTGGCATGGCGATGCGTGCCAAAACGCCAATCTTtga < 1:1401705/51‑1 (MQ=255) gggAAAGGGTCAATGGGGATGGCGATGCGCGCCAAAACGCCAATCTTTGATGACGATGGAAAAGTCATCgg > 1:574689/1‑71 (MQ=255) aaaGGGTCAATGGGGATGGCGATGCGCGCCAAAACGCCAATCTTTGATGACGATGGAAAAGTCATCGGCgt > 1:3586125/1‑71 (MQ=255) aaaGGGTCAATGGGGATGGCGATGCGCGCCAAAACGCCAATCTTTGATGACGATGGAAAAGTCATCGGCgt < 1:607385/71‑1 (MQ=255) aaGGGTCAATGGGGATGGCGATGCGCGCCAAAACGCCAATCTTtgatga < 1:4073854/49‑1 (MQ=255) aaGGGTCAATGGGGATGGCGATGCGCGCCAAAACGCCAATCTTTGATGACGATGGAAAAGTCATCGGCgtg < 1:3087684/71‑1 (MQ=255) tCAATGGGGATGGCGATGCGCGCCAAAACGCCAATCTTTGATGACGATGGAAAAGTCATCGGCGTGGTGTc < 1:514749/71‑1 (MQ=255) tCAATGGGGATGGCGATGCGCGCCAAAACGCCAATCTTTGATGACGATGGAAAAGTCATCGGCGTGGTGTc < 1:1530638/71‑1 (MQ=255) cAATGGGGATGGCGATGCGCGCCAAAACGCCAATc < 1:1203702/35‑1 (MQ=255) cAATGGGGATGGCGATGCGCGCCAAAACGCCAATCTTTGATGACGATGGAAAAGt > 1:813670/1‑55 (MQ=255) aaTTGGCATGGCGATGCGTGCCAAAACGCCAATCTTTGATGACGATGGAAAAGTCATCGGCGTGGTGTCGa > 1:4360123/1‑71 (MQ=255) aaTTGGCATGGCGATGCGTGCCAAAACGCCAATCTTTGATGACGATGGAAAAGTCATCGGCGTGGTGTCGa > 1:2965587/1‑71 (MQ=255) aaTGGGGATGGCGATGCGCGCCAAAACGCCAATCTTTGATGACGATGGaa < 1:2307800/50‑1 (MQ=255) aaTGGGGATGGCGATGCGCGCCAAAACGCCAATCTTTGATGACGATGGAAAAGTCATCGGCGTGGTGTc < 1:4142023/69‑1 (MQ=255) aaTGGGGATGGCGATGCGCGCCAAAACGCCAATCTTTGATGACGATGGAAAAGTCATCGGCGTGGTGTCg < 1:3850860/70‑1 (MQ=255) aaTGGGGATGGCGATGCGCGCCAAAACGCCAATCTTTGATGACGATGGAAAAGTCATCGGCGTGGTGTCGa > 1:206015/1‑71 (MQ=255) aTGGGGATGGCGATGCGCGCCAAAACGCCAATCTTTGATGACGATGGaaa < 1:1116836/50‑1 (MQ=255) tGGGGATGGCGATGCGCGCCAAAACGCCAATCTTTGATGACGATGGAAAAGTCATCGGCGTGGTGTCGa < 1:276059/69‑1 (MQ=255) tGGGGATGGCGATGCGCGCCAAAACGCCAATCTTTGATGACGATGGAAAAGTCATCGGCGTGGTGTCGAtt > 1:989430/1‑71 (MQ=255) ggggATGGCGATGCGCGCCAAAACGCCAATCTTTGATGACGATGGAAAAGTCATc > 1:4258512/1‑55 (MQ=255) | TGCAGTTCACCAAACAGGGCGCGCTGGAGAAAGGGGAGAGCTACTTCATTACCGGGAAAGGGTCAATGGGGATGGCGATGCGCGCCAAAACGCCAATCTTTGATGACGATGGAAAAGTCATCGGCGTGGTGTCGATT > W3110S.gb/651816‑651952 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |