| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 50,527 | C→T | 8.5% | E232E (GAG→GAA) | apaH ← | diadenosine tetraphosphatase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 50,527 | 0 | C | T | 8.5% | 132.6 / 3.1 | 48 | E232E (GAG→GAA) | apaH | diadenosine tetraphosphatase |
| Reads supporting (aligned to +/- strand): ref base C (21/22); new base T (2/2); total (24/24) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.39e-01 | |||||||||||
CAGGTTAATGTACCACCCCAGCAGCAGCCGGTATCCAGCGCGTATATACCTTCCGGCGTACCTTTGCCCTCCAGCGATGCCCAGTGACCAAAGGCGATGCTGTATTCTTCAGCGACAGGGCCAGGAATCGCAAACCAC > W3110S.gb/50459‑50596 | caggTTAATGTACCACCCCAGCAGCAGCCGGTATCCAGCGCGTATATACCTTCCGGCGTACCTTTGCCCt < 1:3966419/70‑1 (MQ=255)caggTTAATGTACCACCCCAGCAGCAGCCGGTATCCAGCGCGTATATACCTTCCGGCGTACCTTTGCCCTc < 1:3087548/71‑1 (MQ=255) aggTTAATGTACCACCCCAGCAGCAGCCGGTATCCAGCGCGTATATACCTTCCGGCGTACCTTTGCCCTc > 1:1802319/1‑70 (MQ=255) ttAATGTACCACCCCAGCAGCAGCCGGTATCCAGCGCGTATATACCTTCCGGCGTACCTTTGCCCTCCAGc < 1:946669/71‑1 (MQ=255) tAATGTACCACCCCAGCAGCAGCCGGTATCCAGCGCGTATATACCTTCCGGCGTACCTTTGCCCTCCAGc > 1:3221188/1‑70 (MQ=255) aaTGTACCACCCCAGCAGCAGCCGGTATCCAGCGCGTATATACCTTCCGGCGTACCTTTGCCCTCCAGCg < 1:198773/70‑1 (MQ=255) ccaccCCAGCAGCAGACGGTATCCAGCGCGTATATACCTTCCGGCGTACCTTTGCCCTCCAGCGa > 1:1662666/1‑65 (MQ=255) gcagcagcCGGTATCCAGCGCGTATATACCTTCCGGCGTACCTTTGCCCTCCAGCGATGCCCAGTGACCa > 1:633231/1‑70 (MQ=255) cagcCGTTATCCAGCGCGTATATACCTTCCGGCGTACCTTTGCCCTCCAGCGATGCCCAGTGACCAAAGGc < 1:3365430/71‑1 (MQ=255) cagcCGGTATCCAGCGCGTATATACCTTCCGGCGTACCTTTGCCCTCCAGCGATGCCCAGTGACCAAAGGc < 1:340133/71‑1 (MQ=255) agcCGGTATCCAGCGCGTATATACCTTCCGGCGTACCTTTGCCCTCCAGCGATGCCCAGTGACCAAAGGc < 1:4374201/70‑1 (MQ=255) agcCGGTATCCAGCGCGTATATACCTTCCGGCGTACCTTTGCCCTCCAGCGATGCCCAGTGACCAAAGGc > 1:375547/1‑70 (MQ=255) agcCGGTATCCAGCGCGTATATACCTTCCGGCGTACCTTTGCCCTCCAGCGATGCCCAGTGACCAAAGGc > 1:2410122/1‑70 (MQ=255) cGGTATCCAGCGCGTAAATACCTTCCGGCGTACCTTTGCCTTCCAGCGATGCCCAGTGACCAAAGGCGATg > 1:657565/1‑71 (MQ=255) gTATCCAGCGCGTATATACCTTCCGGCGTACCTTTGCCCTCCAGCGATGCCCAGTGACCAAAGGCGATGc > 1:1456756/1‑70 (MQ=255) gTATCCAGCGCGTATATACCTTCCGGCGTACCTTTGCCCTCCAGCGATGCCCAGTGACCAAAGGCGATGCt > 1:1677056/1‑71 (MQ=255) gTATCCAGCGCGTATATACCTTCAGGCGTACCTTTGCCCTCCAGCGa > 1:405343/1‑47 (MQ=255) tATCCAGCGCGTATATACCTTCCGGCGTACCTTTGCCCTCCAGCGATGCCCAGTGACCAAAGGCGATGCTg < 1:717163/71‑1 (MQ=255) ccAGCGCGTATATACCTTCCGGCGTACCTTTGCCCTCCAGCGATGCCCAGTGACCAAAGGCGATGCTGTAt > 1:3211087/1‑71 (MQ=255) cAGCGCGTAAATACCTTCCGGCGTACCTTTGCCTTcc > 1:740868/1‑37 (MQ=37) aGCGCGTATATACCTTCCGGCGTACCTTTGCCCTCCAGCGATGCCCAGTg < 1:449598/50‑1 (MQ=255) aGCGCGTATATACCTTCCGGCGTACCTTTGCCCTCCAGCGATGCCCAGTGACCaa < 1:3650893/55‑1 (MQ=255) aGCGCGTATATACCTTCCGGCGTACCTTTGCCCTCCAGCGATGCCCAGTGACCaa < 1:1482152/55‑1 (MQ=255) aGCGCGTATATACCTTCCGGCGTACCTTTGCCCTCCAGCGATGCCCAGTGACCAAAGGCGATGCTGTAttc > 1:4336703/1‑71 (MQ=255) cgcgTATATACCTTCCGGCGTACCTTTGCCCTCCAGCGATGCCCAGTGACCAAAGGCGATGCTGTAttctt < 1:4341370/71‑1 (MQ=255) gTAAATACCTTCCGGCGTACCTTTGCCTTCCAGCGATGCCCAGTGACCAAAGGc < 1:19926/54‑1 (MQ=39) gTAAATACCTTCCGGCGTACCTTTGCCTTCCAGCGATGCCCAGTGACCAAAGGc < 1:762887/54‑1 (MQ=39) tatataCCTTCCGGCGTACCTTTGCCCTCCAGCGATGCCCAGTGACCAAAGGc < 1:527912/53‑1 (MQ=255) tataCCTTCCGGCGTACCTTTGCCGTCCCGCGATGCCCAGTGACCAAAGGCGa > 1:408695/1‑53 (MQ=255) tataCCTTCCGGCGTACCTTTGCCCTCCAGCGATGtcc < 1:3203038/38‑1 (MQ=255) tataCCTTCCGGCGTACCTTTGCCCTCCAGCGATGCCCAGTGACCa < 1:467169/46‑1 (MQ=255) tataCCTTCCGGCGTACCTTTGCCCTCCAGCGATGCCCAGTGACCAAAGGCGATGCTGTa < 1:714358/60‑1 (MQ=255) tataCCTTCCGGCGTACCTTTGCCCTCCAGCGATGCCCAGTGACCAAAGGCGATGCTGTa < 1:4418000/60‑1 (MQ=255) tataCCTTCCGGCGTACCTTTGCCCTCCAGCGATGCCCAGTGACCAAAGGCGATGCTGTATTCTTCAGCGa > 1:309818/1‑71 (MQ=255) tataCCTTCCGGCGTACCTTTGCCCTCCAGCGATGCCCAGTGACCAAAGGCGATGCTGTATTCTTCAGCGa > 1:2920827/1‑71 (MQ=255) tataCCTTCCGGCGTACCTTTGCCCTCCAGCGATGCCCAGTGACCAAAGGCGATGCTGTATTCTTCAGCGa > 1:2265887/1‑71 (MQ=255) tataCCTTCCGGCGTACCTTTGCCCTCCAGCGATGCCCAGTGACCAAAGGCGATGCTGTATTCTTCAGCGa > 1:4415008/1‑71 (MQ=255) taCCTTCCGGCGTACCTTTGCCCTCCAGCGATGCCCAGTGACCAAAGGCGATGCTGTATTCTTCAGCGa > 1:121667/1‑69 (MQ=255) aCCTGCCGGCGTACCTTTGCCCTCCAGCGATGCCCAGTGACCAAAGGCGATGCTGTAt < 1:2806392/58‑1 (MQ=255) ttCCGGCGTACCTTTGCCCTCCAGCGATGCCCAGTGACCAAAGGc < 1:3062820/45‑1 (MQ=255) ccGGCGTACCTTTGCCCTCCAGCGATGCCCAGTGACCAAAGGCGATGCTGTATTCTTCAGCGACAGGGc > 1:3486854/1‑69 (MQ=255) ggCGTACCTTTGCCCTCCAGCGATGCCCAGTGACCAAAGGCGATGCTGTATTCTTCAGCGACAGGGCCAgg < 1:840020/71‑1 (MQ=255) tACCTTTGCCCTCCAGCGATGCCCAGTGACCAAAGGCGATGCTGTATTCTTCAGCGACAGGGCCAgg < 1:482816/67‑1 (MQ=255) cTTTGCCCTCCAGCGATGCCCAGTGACCAAAGGCGATGCTGTATTCTTCAGCGACAGGGCCAGGAATCGCa < 1:4092305/71‑1 (MQ=255) ttGCCCTCCAGCGATGCCCAGTGACCAAAGGCGATGCTGTATTCTTCAGCGACAGGGCCAGGaa > 1:2353053/1‑64 (MQ=255) ttGCCCTCCAGCGATGCCCAGTGACCAAAGGCGATGCTGTATTCTTCAGCGACAGGGCCAGGaa > 1:4055750/1‑64 (MQ=255) ttGCCCTCCAGCGATGCCCAGTGACCAAAGGCGATGCTGTATTCTTCAGCGACAGGGCCAGGAATCGCaaa > 1:1403434/1‑71 (MQ=255) tGCCCTCCAGCGATGCCCAGTGACCAAAGGCGATGCTGTATTCTTCAGCGACAGGGCCAGGAATCGCAAAc > 1:366495/1‑71 (MQ=255) ccTCCAGCGATGCCCAGTGACCAAAGGCGATGCTGTATTCTTCAGCGACAGGGCCAGGAATCGCa < 1:3159028/65‑1 (MQ=255) ccTCCAGCGATGCCCAGTGACCAAAGGCGATGCTGTATTCTTCAGCGACAGGGCCAGGAATCGCAAACCAc < 1:583440/71‑1 (MQ=255) ccTCCAGCGATGCCCAGTGACCAAAGGCGATGCTGTATTCTTCAGCGACAGGGCCAGGAATCGCAAACCAc < 1:2416484/71‑1 (MQ=255) | CAGGTTAATGTACCACCCCAGCAGCAGCCGGTATCCAGCGCGTATATACCTTCCGGCGTACCTTTGCCCTCCAGCGATGCCCAGTGACCAAAGGCGATGCTGTATTCTTCAGCGACAGGGCCAGGAATCGCAAACCAC > W3110S.gb/50459‑50596 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |