Predicted mutation
evidence seq id position mutation freq annotation gene description
RA W3110S.gb 1,303,631 C→T 100% Q246Y (CAG→TAT)  oppA → oligopeptide transporter subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb1,303,6310CT100.0% 226.8 / NA 78Q246Y (CAG→TAT) oppAoligopeptide transporter subunit
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (44/34);  total (44/34)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

AAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATC  >  W3110S.gb/1303567‑1303696
                                                                |                                                                 
aaaCGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTaa                                                             >  1:1340086/1‑71 (MQ=255)
aaaCGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTaa                                                             >  1:2950051/1‑71 (MQ=255)
aaaCGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTaa                                                             >  1:144206/1‑71 (MQ=255)
aaaCGAACGAATCGTTCTTGAACGCAGCACGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTaa                                                             >  1:242730/1‑71 (MQ=255)
  acgaacgaATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAAcc                                                           <  1:118617/71‑1 (MQ=255)
   cgaacgaaTCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAAcc                                                           >  1:2619242/1‑70 (MQ=255)
   cgaacgaaTCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAAc                                                            >  1:1989008/1‑69 (MQ=255)
   cgaacgaaTCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAAc                                                            >  1:2932207/1‑69 (MQ=255)
    gaacgaaTCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTa                                                         <  1:3478084/71‑1 (MQ=255)
    gaacgaaTCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTa                                                         <  1:2847161/71‑1 (MQ=255)
       cgaaTCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTAttt                                                      <  1:1485909/71‑1 (MQ=255)
          aTCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGcc                                                   <  1:185720/71‑1 (MQ=255)
          aTCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGcc                                                   >  1:3372051/1‑71 (MQ=255)
          aTCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGcc                                                   <  1:3045481/71‑1 (MQ=255)
          aTCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGcc                                                   <  1:1159266/71‑1 (MQ=255)
           tCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGcc                                                   >  1:323506/1‑70 (MQ=255)
           tCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCt                                                  <  1:1119282/71‑1 (MQ=255)
            cGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTa                                                 >  1:3246539/1‑71 (MQ=255)
             gTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTAtt                                                       >  1:910736/1‑64 (MQ=255)
             gTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTAtt                                                       >  1:2053802/1‑64 (MQ=255)
             gTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTAt                                                >  1:2630985/1‑71 (MQ=255)
              ttCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTAttt                                                      <  1:997309/64‑1 (MQ=255)
               tCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTAt                                                        >  1:4051734/1‑61 (MQ=255)
                cTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGc                                             >  1:3332579/1‑71 (MQ=255)
                cTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGc                                             >  1:4183239/1‑71 (MQ=255)
                cTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGc                                             >  1:2685004/1‑71 (MQ=255)
                 ttGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCt                                                          <  1:969190/57‑1 (MQ=255)
                 ttGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCt                                            >  1:3410659/1‑71 (MQ=255)
                   gAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGc                                             <  1:3350591/68‑1 (MQ=255)
                   gAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTc                                          >  1:136289/1‑71 (MQ=255)
                     aCGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTg                                        <  1:182731/71‑1 (MQ=255)
                     aCGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTg                                        <  1:2884042/71‑1 (MQ=255)
                      cGCAGCCCGATCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTa                                                 <  1:2239224/61‑1 (MQ=255)
                            ccGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTAc                                 >  1:964558/1‑71 (MQ=255)
                             cGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTAcc                                <  1:2730921/71‑1 (MQ=255)
                                ccTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGAt                             >  1:267764/1‑71 (MQ=255)
                                 cTACTGGAACAACGCGAAAACCGTTATTAACTATGTa                                                              <  1:3825374/37‑1 (MQ=37)
                                   aCTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTa                                                 >  1:2563190/1‑48 (MQ=39)
                                   aCTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTa                                                 >  1:3034713/1‑48 (MQ=39)
                                   aCTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTa                                                 >  1:1233683/1‑48 (MQ=39)
                                   aCTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGt                           >  1:3431531/1‑70 (MQ=255)
                                    cTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTAcc                                <  1:3605358/64‑1 (MQ=255)
                                    cTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTAcc                                <  1:2520326/64‑1 (MQ=255)
                                    cTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTc                          <  1:3999545/70‑1 (MQ=255)
                                    cTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCa                         <  1:1755861/71‑1 (MQ=255)
                                    cTGGAACAACGAGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTAcc                                <  1:149948/64‑1 (MQ=255)
                                       gAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGaa                                      >  1:2288272/1‑55 (MQ=255)
                                       gAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGa                                       >  1:2402769/1‑54 (MQ=255)
                                       gAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAAcc                      >  1:3019702/1‑71 (MQ=255)
                                        aacaacGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGa                              >  1:1441855/1‑62 (MQ=255)
                                        aacaacGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCg                     >  1:1652010/1‑71 (MQ=255)
                                         acaacGCGAAAACCGTTATTAACTATGTAACCTAtt                                                       >  1:173787/1‑36 (MQ=37)
                                         acaacGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGaa                                      >  1:474411/1‑53 (MQ=255)
                                           aacGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTAtt                                               <  1:2114148/42‑1 (MQ=38)
                                            acGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTAc                 <  1:4230355/71‑1 (MQ=255)
                                             cgcgAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGaa                                      >  1:1438360/1‑49 (MQ=39)
                                             cgcgAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTAcc                >  1:2182223/1‑71 (MQ=255)
                                             cgcgAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTAcc                >  1:3892164/1‑71 (MQ=255)
                                              gcgAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTg                                        <  1:4109390/46‑1 (MQ=39)
                                               cgAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGt              <  1:3806908/71‑1 (MQ=255)
                                               cgAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGt              <  1:1485266/71‑1 (MQ=255)
                                                gAAAACCGTTATTACCTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTc                          <  1:3997476/58‑1 (MQ=255)
                                                gAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCg               >  1:2345700/1‑69 (MQ=255)
                                                  aaaCCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTAccta                              >  1:2478927/1‑50 (MQ=255)
                                                  aaaCCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAgt           >  1:4318835/1‑71 (MQ=255)
                                                    aCCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGt                           <  1:1930176/53‑1 (MQ=255)
                                                    aCCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGt                           <  1:1472806/53‑1 (MQ=255)
                                                    aCCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGt                           <  1:4282391/53‑1 (MQ=255)
                                                    aCCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGt                           <  1:724609/53‑1 (MQ=255)
                                                    aCCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAgtgg         >  1:4118830/1‑71 (MQ=255)
                                                        ttattaACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGaa     <  1:370747/71‑1 (MQ=255)
                                                          attaACTATGTAACCTATTTGCCTATTGCTTCTGaa                                      >  1:4065408/1‑36 (MQ=37)
                                                           ttaACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATc  <  1:3831794/71‑1 (MQ=255)
                                                           ttaACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGAGAAAt   <  1:2788589/70‑1 (MQ=255)
                                                            taACTATGTAACCTATTTGCCTATTGCTTCTGAAg                                     >  1:447164/1‑35 (MQ=37)
                                                            taACTATGTAACCTATTTGCCTATTGCTTCTGAAg                                     >  1:3503821/1‑35 (MQ=37)
                                                            taACTATGTAACCTATTTGCCTATTGCTTCTGAAg                                     >  1:3719217/1‑35 (MQ=37)
                                                            taACTATGTAACCTATTTGCCTATTGCTTCTGAAg                                     >  1:1008259/1‑35 (MQ=37)
                                                                |                                                                 
AAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATC  >  W3110S.gb/1303567‑1303696

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: