| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 1,796,459 | G→A | 11.6% | A136A (GCC→GCT) | btuC ← | vitamin B12 transporter subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 1,796,459 | 0 | G | A | 11.6% | 162.1 / 10.5 | 60 | A136A (GCC→GCT) | btuC | vitamin B12 transporter subunit |
| Reads supporting (aligned to +/- strand): ref base G (24/29); new base A (2/5); total (26/34) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.88e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.96e-01 | |||||||||||
TTAGTGCGCTACAGATAATCCCTAATGCAACGCCAGCCAGCAATAACCGACTGGTCGAAAGATGACGACGGGCGAAACGTAAGAGTATTAAAGTGATGATAAGCGCGCCAGCAATCGCACACAGCCCTAGCGCCCAGTTG > W3110S.gb/1796389‑1796528 | ttAGTGCGCTACAGATAATCCCTAATGCAACGCCAGCCAGCAATAACCGACTGGTCGAAAGATGACGACgg > 1:1070313/1‑71 (MQ=255)ttAGTGCGCTACAGATAATCCCTAATGCAACGCCAGCCAGCAATAACCGACTGGTCGAAAGATGACGACgg > 1:1065070/1‑71 (MQ=255) tAGTGCGCTACAGATAATCCCTAATGCAACGCCAGCCAGCAATAACCGACTGGTCGAAAGATGACGACggg > 1:3520762/1‑71 (MQ=255) tGCGCTACAGATAATCCCTAATGCAACGCCAGCCAGCAATAACCGACTGGTCGAAAGATGACGACGGGCGa < 1:1654795/71‑1 (MQ=255) cgcTACAGATAATCCCTAATGCAACGCCAGCCAGCAATAACCGACTGGTCGAAAGATGACGACGGGCGaaa > 1:36785/1‑71 (MQ=255) gcTACAGATAATCCCTAATGCAACGCCAGCCAGCAATAACCGACTGGTCGAAAGATGACGACGGGCGaa < 1:4254852/69‑1 (MQ=255) aCAGATAATCCCTAATGCAACGCCAGCCAGCAATAACCGACTGGTCGAAAGATGACGACGGGCGAAACGt > 1:444850/1‑70 (MQ=255) aCAGATAATCCCTAATGCAACGCCAGCCAGCAATAACCGACTGGTCGAAAGATGACGACGGGCGAAACGt > 1:2698123/1‑70 (MQ=255) cAGATAATCCCTAATGCAACGCCAGCCAGCAATAACCGACTGGTCGAAAGATGACGACGGGCGAAACGTaa < 1:1616057/71‑1 (MQ=255) cAGATAATCCCTAATGCAACGCCAGCCAGCAATAACCGACTGGTCGAAAGATGACGACGGGCGAAACGTa < 1:4388818/70‑1 (MQ=255) aGATAATCCCTAATGCAACGCCAGCCAGCAATAACCGACTGGTCGAAAGATGACGACGGGCGAAACGt > 1:2076198/1‑68 (MQ=255) aTAATCCCTAATGCAACGCCAGCCAGCAATAACCGACTGGTCGAAAGATGACGACGGGCGAAACGTAagag < 1:2247007/71‑1 (MQ=255) aaTCCCTAATGCAACGCCAGCCAGCAATAACCGACTGGTCGAAAGATGACGACGGGCGAAACGTAAGAGTa > 1:3020388/1‑71 (MQ=255) tCCCTAATGCAACGCCAGCCAGCAATAACCGACTGGTCGAAAGATGACGACGGGCGAAACGTAAGAGTAtt < 1:1251284/71‑1 (MQ=255) ccTAATGCAACGCCAGCCAGCAATAACCGACTGGTCGAAAGATGACGACGGGCGAAACGTAAGAGTATTa < 1:1768771/70‑1 (MQ=255) ccTAATCCAACGCCAGCCAGCAATAACCGACTGGTCGAAAGATGACGACGGGCGAAACGTAAGAGTATTa < 1:2855740/70‑1 (MQ=255) cAACGCCAGCCAGCAATAACCGACTGGTCGAAAGATGACGACGGGCGAAACGTAagag > 1:3302723/1‑58 (MQ=255) cAACGCCAGCCAGCAATAACCGACTGGTCGAAAGATGACGACGGGCGAAACGTAagag > 1:552981/1‑58 (MQ=255) cAACGCCAGCCAGCAATAACCGACTGGTCGAAAGATGACGACGGGCGAAACGTAAGAGTATTaaa > 1:3987734/1‑65 (MQ=255) aaCGCCAGCCAGCAATAACCGACTGGTCGAAAGATGACGACGGGCGAAACGTAAGAGTATTAAAGtgatga > 1:3274916/1‑71 (MQ=255) aCGCCAGCCAGCAATAACCGACTGGTCGAAAGATGACGACGGGCGAAACGTAAGAGTATTAAAGTgatgat < 1:799057/71‑1 (MQ=255) ccagcAATAACCGACTGGTCGAAAGATGACGACGAGCGAAACGTAAGAGTATTAAAg > 1:1602998/1‑57 (MQ=255) ccagcAATAACCGACTGGTCGAAAGATGACGACGAGCGAAACGTAAGAGTATTAAAg > 1:3041411/1‑57 (MQ=255) gcAATAACCGACTGGTCGAAAGATGACGACGGGCGAAACGTAAGAGTATTAAAGTGATGATAAgcgcgc > 1:1575404/1‑69 (MQ=255) aaTAACCGACTGGTCGAAAGATGACGACGGGCGAAACTTAAGAGTATTAAAGTGATGATAAGCGCGCCAg > 1:1308495/1‑70 (MQ=255) aaTAACCGACTGGTCGAAAGATGACGACGGGCGAAACGTAAGAGTATTAAAGTGATGATAAGCGCGCCa > 1:1596463/1‑69 (MQ=255) aaTAACCGACTGGTCGAAAGATGACGACGGGCGAAACGTAAGAGTATTAAAGTGATGATAAGCGCGCCAg > 1:2200280/1‑70 (MQ=255) tAACCGACTGGTCGAAAGATGACGACGGGCGAAACGt < 1:3985051/37‑1 (MQ=255) tAACCGACTGGTCGAAAGATGACGACGGGCGAAACGTAAGAGTATTAAAGTGATGATAAGCGCGCCAGCa > 1:1568246/1‑70 (MQ=255) aCCGACTGGTCGAAAGATGACGACGGGCGAAACGTAAGAGTATTaaa > 1:3958269/1‑47 (MQ=255) ccGACTGGTCGAAAGATGACGACGGGCGAAACGTAAGAGTATTAAAGTGATGATAAGCGCGCCAGCAATCg < 1:2729797/71‑1 (MQ=255) ccGACTGGTCGAAAGATGACGACGGGCGAAACGTAAGAGTATTAAAGTGATGATAAGCGCGCCAGCAATCg < 1:3763479/71‑1 (MQ=255) ccGACTGGTCGAAAGATGACGACGGGCGAAACGTAAGAGTATTAAAGTGATGATAAGCGCGCCAGCAATCg < 1:1911316/71‑1 (MQ=255) cGACTGGTCGAAAGATGACGACGGGCGAAACGTAAGAGTAt > 1:3734936/1‑41 (MQ=255) cGACTGGTCGAAAGATGACGACGGGCGAAACGTAAGAGTATTAAAGTGATGATAAGCGCGCCAGCAATCg < 1:1307177/70‑1 (MQ=255) gACTGGTCGAAAGATGACGACGGGCGAAACGTAAGAGTATTAAAGTGATGATAAGCGCGCCAGCAATCGca < 1:563919/71‑1 (MQ=255) gACTGGTCGAAAGATGACGACGGGCGAAACGTAAGAGTATTAAAGTGATGATAAGCGCGCCAGCAATCGca < 1:3551057/71‑1 (MQ=255) gACTGGTCGAAAGATGAAGACGGGCGAAACGTAAGAGTATTAAAGTGATGATAAg < 1:2120181/55‑1 (MQ=255) aCTGGTCGAAAGATGACGACGGGCGAAACGTAAGAGTATTa < 1:52164/41‑1 (MQ=255) aCTGGTCGAAAGATGACGACGAGCGAAACGTAAGAGTATTAAAGTGATGATAAGCGCGCCAGCaa < 1:3551292/65‑1 (MQ=255) aCTGGTCGAAAGATGACGACGAGCGAAACGTAAGAGTATTAAAGTGATGATAAGCGCGCCAGCaa < 1:757780/65‑1 (MQ=255) aCTGGTCGAAAGATGACGACGAGCGAAACGTAAGAGTATTAAAGTGATGATAAGCGCGCCAGCaa < 1:2379426/65‑1 (MQ=255) aCTGGTCGAAAGATGACGACGAGCGAAACGTAAGAGTATTAAAGTGATGATAAGCGCGCCAGCaa < 1:3909338/65‑1 (MQ=255) gTCGAAAGATGACGACGGGCGAAACGTAAGAGTATTAAAGtga > 1:566239/1‑43 (MQ=255) gTCGAAAGATGACGACGGGCGAAACGTAAGAGTATTAAAGTGATGATAAGCGCGCCAGCAATCGcaca > 1:4431569/1‑68 (MQ=255) tCGAAAGATGACGACGGGCGAAACGTAAGAGTATTa < 1:3304851/36‑1 (MQ=255) tCGAAAGATGACGACGGGCGAAACGTAAGAGTATTAAAGTGATGATAAGCGCGCCAGCAAt < 1:3602127/61‑1 (MQ=255) cGAAAGATGACGAAGGGCGAAACGTAAGAGTATTAAAGtga > 1:2834547/1‑41 (MQ=255) aaGATGACGACGGGCGAAACGTAAGAGTATTAAAGTGATGATTAg < 1:1164456/45‑1 (MQ=255) aaGATGACGACGGGCGAAACGTAAGAGTATTAAAGTGATGATAAgc < 1:2374500/46‑1 (MQ=255) aaGATGACGACGGGCGAAACGTAAGAGTATTAAAGTGATGATAAGCGCGCCAGCAATCGCACACAGCCCTa < 1:617636/71‑1 (MQ=255) aGATGACGACGGGCGAAACGTAAGAGTATTAAAGTGATGATAAGCGCGCCAGCAATCGCACACAGCCCTAg > 1:2236385/1‑71 (MQ=255) aGATGACGACGGGCGAAACGTAAGAGTATTAAAGTGATGATAAGCGCGCCAGCAATCGCACACAGCCCTAg > 1:2009422/1‑71 (MQ=255) gATGACGACGAGCGAAACGTAAGAGTATTAAAGTGATGATAAGCGCGCCAGCaa < 1:3154495/54‑1 (MQ=255) aTGACGACGGGCGAAACGTAAGAGTATTAAAGTgatgat < 1:4434879/39‑1 (MQ=255) tGACGACGGGCGAAACGTAAGAGTATTAAAGTGATGATAAGCGCGCCAGCAATCGCACACAg < 1:832778/62‑1 (MQ=255) tGACGACGGGCGAAACGTAAGAGTATTAAAGTGATGATAAGCGCGCCAGCAATCGCACACAGCCCTAgcg > 1:1558343/1‑70 (MQ=255) gacgacGGGCGAAACGTAAGAGTATTAAAGTGATGATAAGCGCGCCAGCAATCGCACACAGc < 1:1877813/62‑1 (MQ=255) acgacgGGCGAAACGTAAGAGTATTAAAGTGATGATAAGCGCGCCAGCAATCGCACACAGCCCTAgc > 1:1235628/1‑67 (MQ=255) acgacgGGCGAAACGTAAGAGTATTAAAGTGATGATAAGCGCGCCAGCAATCGCACACAGCCCTAGCGcc < 1:1156567/70‑1 (MQ=255) acgGGCGAAACGTAAGAGTATTAAAGTGATGATAAGCGCGCCAGCAATCGCACACAGCCCTAGCGCCCAGt < 1:394750/71‑1 (MQ=255) cgGGCGAAACGTAAGAGTATTAAAGTGATGATAAGCGCGCCAGCAATCGcaca > 1:1721196/1‑53 (MQ=255) cgGGCGAAACGTAAGAGTATTAAAGTGATGATAAGCGCGCCAGCAATCGCACACAGCCCTAGCGCCCAGtt < 1:2015252/71‑1 (MQ=255) gggCGAAACGTAAGAGTATTAAAGTGATGATAAGCGCGCCAGCAATCGCACACAGCCCTAGCGCCCAGTTg < 1:3406631/71‑1 (MQ=255) | TTAGTGCGCTACAGATAATCCCTAATGCAACGCCAGCCAGCAATAACCGACTGGTCGAAAGATGACGACGGGCGAAACGTAAGAGTATTAAAGTGATGATAAGCGCGCCAGCAATCGCACACAGCCCTAGCGCCCAGTTG > W3110S.gb/1796389‑1796528 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |