| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 2,193,567 | G→A | 9.0% | R226R (CGC→CGT) | yehB ← | predicted outer membrane protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,193,567 | 0 | G | A | 9.0% | 126.3 / 2.1 | 44 | R226R (CGC→CGT) | yehB | predicted outer membrane protein |
| Reads supporting (aligned to +/- strand): ref base G (21/19); new base A (2/2); total (23/21) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
ACAACCGCACACCTCTGAAGCGAACAGAATCAAAAATATCGCTTGATGTGTACATATCACCCACGCGAAGCGTGCCGAGAAGTTGGGCAAATCCACGTTCCAGATACAGGGTATTGCTTTTCCACACCCCTG > W3110S.gb/2193503‑2193634 | acaacCGCACACCTCTGAAGCGAACAGAATCAAAAATATCGCTTGATGTGTACATATCACCCACGCGAAGc > 1:4322756/1‑71 (MQ=255)acaacCGCACACCTCTGAAGCGAACAGAATCAAAAATATCGCTTGATGTGTACATATCACCCACGCGAAGc > 1:2040894/1‑71 (MQ=255) ccTCTGAAGCGAACAGAATCAAAAATATCGGTTGATGTGTACATATCACCCACGCGAAGCGTGCCGAGAAg > 1:2994282/1‑71 (MQ=255) tGAAGCGAACAGAATCAAAAATATCGCTTGATGTGTACATATCACCCACGCGAAGCGTGCCGAGAAGtt < 1:2133050/69‑1 (MQ=255) tGAAGCGAACAGAATCAAAAATATCGCTTGATGTGTACATATCACCCACGCGAAGCGTGCCGAGAAGtt < 1:359876/69‑1 (MQ=255) tGAAGCGAACAGAATCAAAAATATCGCTTGATGTGTACATATCACCCACGCGAAGCGTGCCGAGAAGTTgg > 1:3807243/1‑71 (MQ=255) tGAAGCGAACAGAATCAAAAATATCGCTTGATGTGTACATATCACCCACGCGAAGCGTGCCGAGAAGTTgg > 1:3552432/1‑71 (MQ=255) gCGAACAGAATCAAAAATATCGCTTGATGTGTACATATCACCCACGCGAAGCGTGCCGAGAAGTTGGGCaa < 1:2846179/71‑1 (MQ=255) aaCAGAATCAAAAATATCGCTTGATGTGTACATATCACCCACGCGAAGCGTGCCGAGAAGTTGGGCAAATc < 1:2225548/71‑1 (MQ=255) cAGAATCAAAAATATCGCTTGATGTGTACATATCACCCACGCGAAGCGTGCCGAGAAGTTGGGCAAATCCa > 1:893708/1‑71 (MQ=255) cAGAATCAAAAATATCGCTTGATGTGTACATATCACCCACGCGAAGCGTGCCGAGAAGTTGGGCAAATCCa < 1:3851322/71‑1 (MQ=255) cAGAATCAAAAATATCGCTTGATGTGTACATATCACCCACGCGAAGCGTGCCGAGAAGTTGGGCAAATCCa > 1:1095168/1‑71 (MQ=255) gAATCAAAAATATCGCTTGATGTGTACATATCACCCACGCGAAGCGTGCCGAGAAGTTGGGCaa < 1:1978056/64‑1 (MQ=255) gAATCAAAAATATCGCTTGATGTGTACATATCACCCACGCGAAGCGTGCCGAGAAGTTGGGCAAAt > 1:1415107/1‑66 (MQ=255) gAATCAAAAATATCGCTTGATGTGTACATATCACCCACGCGAAGCGTGCCGAGAAGTTGGGCAAATCCACg > 1:2203150/1‑71 (MQ=255) tCAAAAATATCGCTTGATGTGTACATATCACCCACGCGAAGCGTGCCGAGAAGTTGGGCAAATCCACGTTc > 1:3698127/1‑71 (MQ=255) aaaaaTATCGCTTGATGTGTACATATCACCCACGCGAAGCGTGCCGAGAAGTTGGGCAAATCCa > 1:2334106/1‑64 (MQ=255) aaTATCGCTTGATGTGTACATATCACCCACGCGAAGCGTGCCGAGAAGTTGGGCAAATCCACGTTCCAGAt < 1:2682629/71‑1 (MQ=255) atatCGCTTGATGTGTACATATCACCCACGCGAAGCGTGCCGAGAAGTTGGGCAAATcc < 1:1837626/59‑1 (MQ=255) atatCGCTTGATGTGTACATATCACCCACGCGAAGCGTGCCGAGAAGTTGGGCAAATCCACGTTCCAGATa < 1:3027937/71‑1 (MQ=255) gCTTGATGTGTACATATCACCCACGCGAAGCGTGCCgaga < 1:1342807/40‑1 (MQ=255) gATGTGTACATATCACCCACGCGAAGCGTGCCGAGAAGTTGGGCAAATCCACGTTCCAGATACAGGGTa < 1:3030276/69‑1 (MQ=255) gATGTGTACATATCACACACGCGAAGCGTGCCGAGAAGTTGGGCAAATCCACGTTCCAGATACAGGGTAt < 1:1025453/70‑1 (MQ=255) aTGTGTACATATCACCCACGCGAAGCGTGCCGAGAAGTTGGGCAAATCCACGTTCCAGATACAGGGTATTg > 1:1026299/1‑71 (MQ=255) aTGTGTACATATCACCCACGCGAAGCGTGCCGAGAAGTTGGGCAAATCCACGTTCCAGATACAGGGTATTg > 1:2643627/1‑71 (MQ=255) aTGTGTACATATCACCCACGCGAAGCGTGCCGAGAAGTTGGGCAAATCCACGTTCCAGATACAGGGTATTg > 1:3625353/1‑71 (MQ=255) tgtACATATCACCCACGCGAAGCGTGCCGAGAAGTTGGGCAAATCCACGTTCCa < 1:3624642/54‑1 (MQ=255) tACATATCACCCACGCGAAGCGTGCCGAGAAGTTGGGCAAATCCACGTTCCa < 1:3021163/52‑1 (MQ=255) tACATATCACCCACGCGAAGCGTGCCGAGAAGTTGGGCAAATCCACGTTCCa < 1:3762931/52‑1 (MQ=255) tACATATCACCCACGCGAAGCGTGACGAGAAGTTGGGCAAATCCACGTTCCAGAt < 1:2586553/55‑1 (MQ=255) cATATCACCCACGCGAAGCGTGCCTAGAAGTTGGGCAAATCCACGTTCCAGATACAGGGTATTGCTTTTc < 1:398224/70‑1 (MQ=255) cATATCACCCACGCGAAGCGTGCCGAGAAGTTGGGCAAATCCACGTTCCAGATACAGGGTATTGCTTTTc > 1:1886528/1‑70 (MQ=255) cATATCACCCACACGAAGCGTGCCGAGAAGTTGGGCAAAt > 1:1453370/1‑40 (MQ=255) atatCACCCACGCGAAGCGTGCCGAGAAGTTGGGCAAATCCACGTTCCAGATACAGGGTATTGCTTTTCca > 1:562496/1‑71 (MQ=255) tatCACCCACGCGAAGCGTGCCGAGAAGTTGGGCAAATCCACGTTCCAg > 1:4396283/1‑49 (MQ=255) tatCACCCACGCGAAGCGTGCCGAGAAGTTGGGCAAATCCACGTTCCAGa > 1:198182/1‑50 (MQ=255) tatCACCCACGCGAAGCGTGCCGAGAAGTTGGGCAAATCCACGTTCCAGa > 1:715892/1‑50 (MQ=255) tatCACCCACACGAAGCGTGCCGAGAAGTTGGGCAAATCCACGTTCCAGATACAGGGTAt < 1:2491543/60‑1 (MQ=255) tatCACCCACACGAAGCGTGCCGAGAAGTTGGGCAAATCCACGTTCCAGATACAGGGTAt < 1:2802266/60‑1 (MQ=255) tCACCCACGCGAAGCGTGCCGAGAAGTTGGGCAAATCCACGTTCCAGATACAGGGTATTGCTTTTCcaca > 1:2423509/1‑70 (MQ=255) tCACCCACACGAAGCGTGCCGAGAAGTTGGGCAAATCCACGTTCCAGATACAGGGTATTGCTTTTCcaca > 1:2119397/1‑70 (MQ=255) ccACGCGAAGCGTGCCGAGAAGTTGGGCAAATCCACGTTCCAGATACAgg < 1:382105/50‑1 (MQ=255) cACGCGAAGCGTGCCGAGAAGTTGGGCAAATCCACGTTCCAGATACAGGGTATTGCTTTTCCACACCCCTg < 1:4286558/71‑1 (MQ=255) cACGCGAAGCGTGCCGAGAAGTTGGGCAAATCCACGTTCCAGATACAGGGTATTGCTTTTCCACACCCCTg > 1:184440/1‑71 (MQ=255) gcgAAGCGTGCCGAGAAGTTGGGCAAATCCTCGTTCCAGATACAGGGTATTGCTTTTCCACAcc < 1:1645123/64‑1 (MQ=255) gcgAAGCGTGCCGAGAAGTTGGGCAAATCCACGTTCCAGATACAGGGTATTGCTTTTCCACAcc < 1:2607858/64‑1 (MQ=255) | ACAACCGCACACCTCTGAAGCGAACAGAATCAAAAATATCGCTTGATGTGTACATATCACCCACGCGAAGCGTGCCGAGAAGTTGGGCAAATCCACGTTCCAGATACAGGGTATTGCTTTTCCACACCCCTG > W3110S.gb/2193503‑2193634 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |