| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 2,893,190 | A→G | 5.2% | S113S (TCA→TCG) | ygcP → | predicted anti‑terminator regulatory protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,893,190 | 0 | A | G | 5.2% | 210.6 / 2.6 | 77 | S113S (TCA→TCG) | ygcP | predicted anti‑terminator regulatory protein |
| Reads supporting (aligned to +/- strand): ref base A (34/39); new base G (2/2); total (36/41) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.91e-01 | |||||||||||
AATGTTGAAAGCGGCAAGAGCGGAAGGTTTTTTCTGTATTCATCGCCTGTTTATTGTTGATTCAATTTCGTTTCACAACATTGATAAGCAAGTTGCGCAATCGAATCCGGATTGTATTGAGATCCTGCCAGGCT > W3110S.gb/2893127‑2893260 | aaTGTTGAAAGCGGCAAGAGCGGAAGGTTTTTTCTGTATTCATCGCCTGTTTATTGTTGATT‑AATTTCg < 1:4388546/69‑1 (MQ=255) tGTTGAAAGCGGCAAGAGCGGAAGGTTTTTTCTGTATTCATCGCCTGTTTATTGTTGATTCAATTTCGttt < 1:3692789/71‑1 (MQ=255) tGAAAGCGGCAAGAGCGGAAGGTTTTTTCTGTATTCATCGCCTGTTTATTGTTGATTCAATTTCGTTTca > 1:4126442/1‑70 (MQ=255) gAAAGCGGCAAGAGCGGAAGGTTTTTTCTGTATTCATCGCCTGTTTATTGTTGATTCAAt > 1:1595618/1‑60 (MQ=255) gAAAGCGGCAAGAGCGGAAGGTTTTTTCTGTATTCATCGCCTGTTTATTGTTGATTCAATTTCGTTTcac > 1:3590478/1‑70 (MQ=255) gAAAGCGGCAAGAGCGGAAGGTTTTTTCTGTATTCATCGCCTGTTTATTGTTGATTCAATTTCGTTTc < 1:1105622/68‑1 (MQ=255) cGGCAAGAGCGGAAGGTTTTTTCTGTATTCATCGCCTGTTTATTGTTGATTCAATTTCGTTTCACAACAtt > 1:4255328/1‑71 (MQ=255) ggCAAGAGCGGAAGGTTTTTTCTGTATTCATCGCCTGTTTATTGTTGATTCAATTTCGTTTCACAACATTg < 1:1355817/71‑1 (MQ=255) ggCAAGAGCGGAAGGTTTTTTCTGTATTCATCGCCTGTTTATTGTTGATTCAATTTCGTTTCACAACATTg < 1:528554/71‑1 (MQ=255) ggCAAGAGCGGAAGGTTTTTTCTGTATTCATCGCCTGTTTATTGTTGATTCAATTTCGTTTCACAACATTg < 1:4093589/71‑1 (MQ=255) gCAAGAGCGGAAGGTTTTTTCTGTATTCATCGCCTGTTTATTGTTGATTCAATTTCGTTTCACAACATTGa > 1:380587/1‑71 (MQ=255) gCAAGAGCGGAAGGTTTTTTCTGTATTCATCGCCTGTTTATTGTTGATTCAATTTCGTTTCACAACATTGa > 1:2448097/1‑71 (MQ=255) agagCGGAAGGTTTTTTCTGTATTCATCGCCTGTTTATTGTTGATTCAATTTCGTTTCACAACATTGATaa < 1:1973996/71‑1 (MQ=255) aaGGTTTTTTCTGTATTCATCGCCTGTTTATTGTTGATTCAATTTCGTTTCACAACATTGATAAGCAAGtt < 1:3925979/71‑1 (MQ=255) aGGTTTTTTCTGTATTCATCGCCTGTTTATTGTTGATTCAATTTCGTTTCACAACATTGATAAGCAAGTTg > 1:2893280/1‑71 (MQ=255) ggTTTTTTCTGTATTCATCGCCTGTTTATTGTTGATTCAATTTCGTTTCacaa > 1:3259596/1‑53 (MQ=255) ttttttCTGTATTCATCGCCTGTTTATTGTTGATTCAAtt < 1:3371729/40‑1 (MQ=255) ttttttCTGTATTCATCGCCTGTTTATTGTTGATTCAATTTCGTTTca < 1:273237/48‑1 (MQ=255) tttttCTTTATTCATCGCCTGTTTATTGTTGATTCAATTTCGTTTCACAACATTGATAAGCaa > 1:2089105/1‑63 (MQ=255) tttttCTGTATTCATCGCCTGTTTATTGTTGATTCAATTTCGTTTCACAACATTGATAAGCAAGTTgcgc > 1:2936460/1‑70 (MQ=255) tttCTGTATTCATCGCGTGTTTATTGTTGATTCAATTTCGTTTCACAACATTGATAAGCAAGTTGCGCaa < 1:2208324/70‑1 (MQ=255) tttATTCATCGCCTGTTTATTGTTGATTCAATTTCGTTTCACAACATTGATAAGCa < 1:3779451/54‑1 (MQ=255) tATTCATCGCCTGTTTATTGTTGATTCAATTTCGt < 1:1992606/35‑1 (MQ=255) tATTCATCGCCTGTTTATTGTTGATTCAATTTCGt < 1:3213452/35‑1 (MQ=255) tATTCATCGCCTGTTTATTGTTGATTCAATTTCGt < 1:3208588/35‑1 (MQ=255) tATTCATCGCCTGTTTATTGTTGATTCAATTTCGt < 1:2396270/35‑1 (MQ=255) tATTCATCGCCTGTTTATTGTTGATTCAATTTCGTTTCACAACATTGa < 1:1640906/48‑1 (MQ=255) tATTCATCGCCTGTTTATTGTTGATTCAATTTCGTTTCACAACATTGATAAGCAAGTTGCGCAATCGAAt < 1:3393626/70‑1 (MQ=255) aTTCATCGCCTGTTTATTGTTGATTCAATTTCGTTTCACAACATTGATAAGCAAGTTGCGCAATCGAATcc > 1:125441/1‑71 (MQ=255) tCATCGCCTGTTTATTGTTGATTCAATTTCGTTTCACAACATTGATAAGCa < 1:1739758/51‑1 (MQ=255) tCATCGCCTGTTTATTGTTGATTCAATTTCGTTTCACAACATTGATAAGCa < 1:3681778/51‑1 (MQ=255) cATCGCCTGTTTATTGTTGATTCAATTTCGTTTCACAACATTGAt < 1:2244729/45‑1 (MQ=255) cATCGCCTGTTTATTGTTGATTCAATTTCGTTTCACAACATTGAt > 1:3676156/1‑45 (MQ=255) cATCGCCTGTTTATTGTTGATTCAATTTCGTTTCACAACATTGATAAGCAAGTTCCGCAATCGa > 1:2401080/1‑64 (MQ=255) aTCGCCTGTTTATTGTTGATTCAATTTCGTTTCACAACATTGATAAGCAAGTTGCGCAATCGAATCCGGa < 1:860687/70‑1 (MQ=255) aTCGCCTGTTTATTGTTGATTCAATTTCGTTTCACAACATTGATAAGCAAGTTGCGCAATCGAATCCGGa < 1:1453643/70‑1 (MQ=255) tCGCCTGTTTATTGTTGATTCAATTTCGTTTCACAACATTGATAAGCaa < 1:3980659/49‑1 (MQ=255) tCGCCTGTTTATTGTTGATTCAATTTCGTTTCACAACATTGATAAGCaa < 1:890327/49‑1 (MQ=255) tCGCCTGTTTATTGTTGATTCAATTCCGTTTCACAACATTg < 1:2731821/41‑1 (MQ=255) cGCCTGTTTATTGTTGATTCAATTTCGTTTCACAACATTGATAAGCAAGTTGCGCAATCGAATCCGGATTg > 1:2235593/1‑71 (MQ=255) gCCTGTTTATTGTTGATTCAATTTCGTTTCACAACATTGATAAGCAAGTTGCGCAATCGAATCCGGATTg < 1:132501/70‑1 (MQ=255) gCCTGTTTATTGTTGATTCAATTTCGTTTCACAACATTGATAAGCAAGTTGCGCAATCGAATCCGGATTGt < 1:3614814/71‑1 (MQ=255) cTGTTTATTGTTGATTCAATTTCGTTTCACAACATTGATAAGCAAGTTGCTCAATCGAATCCGGATTGTAt < 1:1292301/71‑1 (MQ=255) tGTTTATTGTTGATTCGATTTCGTTTCACAACATTGATAAGCAAGTTGCGCAATCGAATCCGGATTg < 1:58777/67‑1 (MQ=255) tGTTTATTGTTGATTCGATTTCGTTTCACAACATTGATAAGCAAGTTGCGCAATCGAATCCGGATTg < 1:4422084/67‑1 (MQ=255) gTTTATTGTTGATTCAATTTCGTTTCACAACATTGATAAGCAAGTTGCGCAATCGAATCCGGATTGTAtt > 1:2797780/1‑70 (MQ=255) tttATTGTTGATTCAATTTCGTTTCACAACATTGATAAGCa < 1:3184333/41‑1 (MQ=255) tttATTGTTGATTCAATTTCGTTTCACAACATTGATAAGCa < 1:744481/41‑1 (MQ=255) tttATTGTTGATTCAATTTCGTTTCACAACATTGATAAGCAAGt < 1:2691241/44‑1 (MQ=255) tttATTGTTGATTCAATTTCGTTTCACAACATTGATAAGCAAGTTGCGCAATCGAATCCgg < 1:1088823/61‑1 (MQ=255) tttATTGTTGATTCAATTTCGTTTCACAACATTGATAAGCAAGTTGCGCAATCGAATCCGGa < 1:4407446/62‑1 (MQ=255) tttATTGTTGATTCAATTTCGTTTCACAACATTGATAAGCAAGTTGCGCAATCGAATCCGGATTGTAtt > 1:1000982/1‑69 (MQ=255) tttATTGTT‑ATTCAATTTCGTTTCACAACATTGATAAGCAAGTTGCGCAATCGAATCCGGATTGTAtt > 1:1228342/1‑68 (MQ=255) tATTGTTGATTCGATTTCGTTTCACAACATTGATAAGCAAGTTGCGCAATCGAATCCGGATTGt > 1:1265457/1‑64 (MQ=255) tATTGTTGATTCGATTTCGTTTCACAACATTGATAAGCAAGTTGCGCAATCGAATCCGGATTGt > 1:974383/1‑64 (MQ=255) tATTGTTGATTCAATTTCGTTTCACAACATTGATAAGCAAGtt > 1:3514640/1‑43 (MQ=255) tATTGTTGATTCAATTTCGTTTCACAACATTGATAAGCAAGTTGCGGAATCGAATCCGGAt > 1:462649/1‑61 (MQ=255) tATTGTTGATTCAATTTCGTTTCACAACATTGATAAGCAAGTTGCGCAAt > 1:2031044/1‑50 (MQ=255) tATTGTTGATTCAATTTCGTTTCACAACATTGATAAGCAAGTTGCGCAATCGAATCCGGa > 1:237710/1‑60 (MQ=255) tATTGTTGATTCAATTTCGTTTCACAACATTGATAAGCAAGTTGCGCAATCGAATCCGGATTg < 1:3105194/63‑1 (MQ=255) tATTGTTGATTCAATTTCGTTTCACAACATTGATAAGCAAGTTGCGCAATCGAATCCGGATTGTAtt > 1:1877125/1‑67 (MQ=255) tATTGTTGATTCAATTTCGTTTCACAACATTGATAAGCAAGTTGCGCAATCGAATCCGGATTGTATTgag < 1:2559812/70‑1 (MQ=255) ttgttgATTCAATTTCGTTTCACAACATTGATAAGCAAGTTGCGCAATCGAATCCGGATTGTATTgag < 1:717822/68‑1 (MQ=255) ttgttgATTCAATTTCGTTTCACAACATTGATAAGCAAGTTGCGCAATCGAATCCGGATTGTATTGAGAt < 1:3477785/70‑1 (MQ=255) tgATTCAATTTCGTTTCACAACATTGATAAGCAAGTTGCGCAATCGAATCCGGATTGTATTGAGATCCTGc > 1:1677367/1‑71 (MQ=255) gATTCAATTTCGTTTCACAACATTGATAAGCAAGTTGCGCAATc > 1:2909057/1‑44 (MQ=255) gATTCAATTTCGTTTCACAACATTGATAAGCAAGTTGCGCAATCGAATCCGGATTGTa > 1:1993675/1‑58 (MQ=255) gATTCAATTTCGTTTCACAACATTGATAAGCAAGTTGCGCAATCGAATCCGGATTGTAt > 1:977766/1‑59 (MQ=255) gATTCAATTTCGTTTCACAACATTGATAAGCAAGTTGCGCAATCGAATCCGGATTGTATTGAGATCCttc > 1:1021062/1‑68 (MQ=255) gATTCAATTTCGTTTCACAACATTGATAAGCAAGTTGCGCAATCGAATCCGGATTGTATTGAGATCCTGc < 1:1206282/70‑1 (MQ=255) ttCAATTTCGTTTCACAACATTGATAAGCAAGTTGCGCAATCGAATCCGGATTGTATTgaga > 1:1753451/1‑62 (MQ=255) ttCAATTTCGTTTCACAACATTGATAAGCAAGTTGCGCAATCGAATCCGGATTGTATTGAGATCCTGCCAg > 1:2337562/1‑71 (MQ=255) ttCAATTTCGTTTCACAACATTGATAAGCAAGTTGCGCAATCGAATCCGGATTGTATTGAGATCCTGCCAg > 1:1251989/1‑71 (MQ=255) tCAATTTCGTTTCACAACATTGATAAGCAAGTTGCGCAATCGAATCCGGATTGTATTGAGATCCTGCCAgg > 1:1512748/1‑71 (MQ=255) cAATTTCGTTTCACAACATTGATAAGCAAGTTGCGCAATCGaa > 1:3562163/1‑43 (MQ=255) cAATTTCGTTTCACAACATTGATAAGCAAGTTGCGCAATCGAATCCGGATTGTATTGAGAt > 1:1059566/1‑61 (MQ=255) cAATTTCGTTTCACAACATTGATAAGCAAGTTGCGCAATCGAATCCGGATTGTATTGAGATCCTGCCAGGc > 1:425496/1‑71 (MQ=255) aaTTTCGTTTCACAACATTGATAAGCAAGTTGCGCAATCGaa > 1:4151392/1‑42 (MQ=255) aaTTTCGTTTCACAACATTGATAAGCAAGTTGCGCAATCGAATCCGGATTGTATTGAGATCCTGCCAGGCt < 1:3891586/71‑1 (MQ=255) aaTTTCGTTTCACAACATTGATAAGCAAGTTGCGCAATCGAATCCGGATTGTATTGAGATCCTGCCAGGCt < 1:2646962/71‑1 (MQ=255) | AATGTTGAAAGCGGCAAGAGCGGAAGGTTTTTTCTGTATTCATCGCCTGTTTATTGTTGATTCAATTTCGTTTCACAACATTGATAAGCAAGTTGCGCAATCGAATCCGGATTGTATTGAGATCCTGCCAGGCT > W3110S.gb/2893127‑2893260 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |