| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 2,897,694 | T→C | 7.7% | E127E (GAA→GAG) | ygcU ← | predicted FAD containing dehydrogenase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,897,694 | 0 | T | C | 7.7% | 131.1 / 3.3 | 52 | E127E (GAA→GAG) | ygcU | predicted FAD containing dehydrogenase |
| Reads supporting (aligned to +/- strand): ref base T (18/30); new base C (2/2); total (20/32) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.34e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.23e-01 | |||||||||||
CGCCAGCGGCTTTGACTGCGGAGAATGCCCCGTGGTGTAACCTTTTTCACGCAACGCGTTTTCCAGCACTTCCAGCGGAACACCACATTGCGCCGTCGCCTGCATATTCTCAATATCAATATTAATGATTTGATTCAT > W3110S.gb/2897625‑2897762 | cgcCAGCGGCTTTGACTGCGGAGAATG‑‑CCGTGGTGTAACCTTTTTCACGCAACGCGTTTTCCAGCACTTcc < 1:2313170/71‑1 (MQ=255) gcCAGCGGCTTTGACTGCGGAGAATGCCCCGTGGTGTAACCTTTTTCACGCAACGCGTTTTCCAGCACTTc > 1:1281668/1‑71 (MQ=255) ccAGCGGCTTTGACTGCGGAGAATGCCCCGTGGTGTAACCTTTTTCACGCAACGCGTTTTCCAGCACttac < 1:2960820/71‑3 (MQ=255) ccAGCGGCTTTGACTGCGGAGAATGCCCCGTGGTGTAACCTTTTTCACGCAACGCGTTTTCCAGCACTTc > 1:1382601/1‑70 (MQ=255) aGCGGCTTTGACTGCGGAGAATGCCCCGTGGTGTAACCTTTTTCACGCAACGCGTTTTCCAGCACTTCCAg < 1:1698583/71‑1 (MQ=255) gCGGCTTTGACTGCGGAGAATGCCCCGTGGTGTAACCTTTTTCACGCAACGCGTTTTCCAGCACTTCCAga < 1:148446/71‑2 (MQ=255) gCGGCTTTGACTGCGGAGAATGCCCCGTGGTGTAACCTTTTTCACGCAACGCGTTTTCCAGCACTTCCAg < 1:866969/70‑1 (MQ=255) ggCTTTGACTGCGGAGAATGCCCCGTGGTGTAACCTTTTTCACGCAACGCGTTTTCCAGCACTTCCAGCgg < 1:707822/71‑1 (MQ=255) ggCTTTGACTGC‑‑AGAATGCCCCGTGGTGTAACCTTTTTCACGCAACGCGTTTTCCAGCACTTCCAGCGGa > 1:1623171/1‑70 (MQ=255) tGACTGCGGAGAATGCCCCGTGGTGTAACCTTTTTCACGCAACGCGTTTTCCAGCACTTCCAg < 1:1052378/63‑1 (MQ=255) tGACTGCGGAGAATGCCCCGTGGTGTAACCTTTTTCACGCAACGCGTTTTCCAGCACTTCCAGCGGAaca < 1:819952/70‑1 (MQ=255) gACTGCGGAGAATGCCCCGTGGTGTAACCTTTTTCACGCAACGCGTTTTCCAGCACTTCCAGCGGAacac < 1:804918/70‑1 (MQ=255) aCTGCGGAGAATGCCCCGTGGTGTAACCTTTTTCACGCAACGCGTTTTCCAGCACTTCCAGCg > 1:1289295/1‑63 (MQ=255) tGCGGAGAATGCCCCGTGGTGTAACCTTTTTCACGCAACGCGTTTTCCAGCACTTCCAGCGGaa < 1:2213778/64‑1 (MQ=255) cGGAGAATGCCCCGTGGTGTAACCTTTTTCACGCAACGCGTTTTCCAGCACTTCCAGCGGAACACcaca < 1:1810594/69‑1 (MQ=255) cGGAGAATGCCCCGTGGTGTAACCTTTTTCACGCAACGCGTTTTCCAGCACTTCCAGCGGAACACCACAtt < 1:3121492/71‑1 (MQ=255) cccGTGGTGTAACCTTTTTCACGCAACGCGTTTTCCAGCACCTCCAGCGGAACGCCACATTGCGCCGTGGc < 1:260626/71‑1 (MQ=255) cccGTGGTGTAACCTTTTTCACGCAACGCGTTTTCCAGCACCTCCAGCGGAACGCCACATTGCGCCGTGGc < 1:1161142/71‑1 (MQ=255) gtggtgTAACCTTTTTCACGCAACGCGTTTTCCAGCACCTCCAGCGGAACGCCACATTGCGCCGTGGCCTg > 1:222469/1‑71 (MQ=255) gtggtgTAACCTTTTTCACGCAACGCGTTTTCCAGCACCTCCAGCGGAACGCCACATTGCGCCGTGGCCTg > 1:29706/1‑71 (MQ=255) tggtgTAACCTTTTTCACGCAACGCGTTTTCCAGCACTTCCAGCGGAACACCACATTGCGCCg < 1:4416041/63‑1 (MQ=255) tggtgTAACCTTTTTCACGCAACGCGTTTTCCAGCACTTCCAGCGGAACACCACATTGCGCCGTCGCCTg > 1:1728696/1‑70 (MQ=255) tggtgTAACCTTTTTCACGCAACGCGTTTTCCAGCACTTCCAGCGGAACACCACATTGCGCCGTCGCCTg > 1:4347363/1‑70 (MQ=255) gtgtAACCTTTTTCACGCAACGCGTTTTCCAGCACTTCCAGCGGAACACCACATTGCGCCGTCGCCTGCa < 1:2776690/70‑1 (MQ=255) tAACCTTTTTCACGCAACGCGTTTTCCAGCACTTCCAGCGGAAcacca < 1:2008696/48‑1 (MQ=255) tAACCTTTTTCACGCAACGCGTTTTCCAGCACTTCCAGCGGAAcacca < 1:1655881/48‑1 (MQ=255) tAACCTTTTTCACGCAACGCGTTTTCCAGCACTTCCAGCGGAAcacca < 1:1610150/48‑1 (MQ=255) tAACCTTTTTCACGCAACGCGTTTTCCAGCACTTCCAGCGGAACACCACATTGCGCCGTCGcc > 1:3125274/1‑63 (MQ=255) aCCTTTTTCACGCAACGCGTTTTCCAGCACTTCCAGCGGAacac < 1:2119816/44‑1 (MQ=255) aCCTTTTTCACGCAACGCGTTTTCCAGCACTTCCAGCGGAACACcaca > 1:556919/1‑48 (MQ=255) aCCTTTTTCACGCAACGCGTTTTCCAGCACTTCCAGCGGAACACCACATTGCGCCGTCGCCTGCATATtct > 1:2234318/1‑71 (MQ=255) aCCTTTTTCACGCAACGCGTTTTCCAGCACTTCCAGCGGAACACCACATTGCGCCGTCGCCTGCATATtct > 1:3024536/1‑71 (MQ=255) ccTTTTTCACGCAACGCGTTTTCCAGCACTTCCAGCGGAACACCACATTGCGCCGTCGCCTGCATATtctc < 1:3429951/71‑1 (MQ=255) cTTTTTCACGCAACGCGTTTTCCAGCACTTCCAGCGGAACACCACATTGCGCCGTCGCCTg < 1:2240662/61‑1 (MQ=255) cTTTTTCACGCAACGCGTTTTCCAGCACTTCCAGCGGAACACCACATTGCGCCGTCGCCTGCATATtctc > 1:2380698/1‑70 (MQ=255) tttttCACGCAACGCGTTTTCCAGCACTTCCAGCGGAacac > 1:827767/1‑41 (MQ=255) ttttCACGCAACGCGTTTTCCAGCACTTCCAGCGGAACACCACATTGCGCCGTCGCCTGCa > 1:487777/1‑61 (MQ=255) tttCACGCAACGCGTTTTCCAGCACTTCCAGCGGAACACCACATTGCGCCGTCGCCTGCatat > 1:1122412/1‑63 (MQ=255) tttCACGCAACGCGTTTTCCAGCACTTCCAGCGGAACACCACATTGCGCCGTCGCCTGCatat > 1:1530306/1‑63 (MQ=255) tCACGCAACGCGTTTTCCAGCACTTCCAGCGGAACACCACATTGCGCCGTCGCCTGCATATTCtcaatat < 1:898369/70‑1 (MQ=255) cACGCAACGCGTTTTCCAGCACTTCCAGCGGAACACCACATTGCGCCGTCGCCTGCATATTCtcaatatc < 1:2701145/70‑1 (MQ=255) cACGCAACGCGTTTTCCAGCACTTCCAGCGGAACACCACATTGCGCCGTCGCCTGCATATTCtcaatatc > 1:2001845/1‑70 (MQ=255) cAACGCGTTTTCCAGCACTTCCAGCGGAACACCACATTGCGCCGTCGCCTGCATATTCTcaatatcaatat < 1:4277807/71‑1 (MQ=255) aCGCGTTTTCCAGCACTTCCAGCGGAACACCACATTGCGCCGTCGCCTGCATATtctc < 1:811008/58‑1 (MQ=255) aCGCGTTTTCCAGCACTTCAAGCGGAACACCACATTGCGCCGTCGCCTGCATATtctc < 1:4077669/58‑1 (MQ=255) cgcgTTTTCCAGCACTTCCAGCGGAACACCACATTGCGCCGTCGCCTGCATATTCTCAATATCAATATTaa > 1:2601193/1‑71 (MQ=255) cgTTTTCCAGCACTTCCAGCGGAACACCACATTGCGCCGTCGCCTGCATATTCTCAATATCAATATTa < 1:3264145/68‑1 (MQ=255) ttttCCAGCACTTCCAGCGGAACACCACATTGCGCCGTCGCCTGCATATTCtcaatatcaat < 1:2322407/62‑1 (MQ=255) ttCCAGCACTTCCAGCGGAACACCACATTGCGCCGTCGCCTGCATATTCTCAATATCAATATTAAtgattt > 1:4326314/1‑71 (MQ=255) tCCAGCACTTCCAGCGGAACACCACATTGCGCCGTCGCCTGCATATTCTCAATATCAATATTAAtgattt < 1:2878113/70‑1 (MQ=255) tCCAGCAATTCCAGCGGAACACCACATTGCGCCGTCGCCTGCATATTCTCAATATCAATATTAAtgattt < 1:1234986/70‑1 (MQ=255) aCTTCCAGCGGAACACCACATTGCGCCGTCGCCTGCATATTCTCAATATCAATATTAATGATTTGATTCAt < 1:1797788/71‑1 (MQ=255) cTTCCAGCGGAACACCACATTGCGCCGTCGCCTGCATATTCTCAATATCAATATTAAtgatttgatt < 1:1582520/67‑1 (MQ=255) ttCCAGCGGAACACCACATTGCGCCGTCTCCTGCATATTCTCAATATCAATATTAAtgatttg < 1:2148540/63‑1 (MQ=255) | CGCCAGCGGCTTTGACTGCGGAGAATGCCCCGTGGTGTAACCTTTTTCACGCAACGCGTTTTCCAGCACTTCCAGCGGAACACCACATTGCGCCGTCGCCTGCATATTCTCAATATCAATATTAATGATTTGATTCAT > W3110S.gb/2897625‑2897762 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |