| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 2,897,721 | C→G | 7.8% | A118A (GCG→GCC) | ygcU ← | predicted FAD containing dehydrogenase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,897,721 | 0 | C | G | 7.8% | 141.4 / 2.2 | 51 | A118A (GCG→GCC) | ygcU | predicted FAD containing dehydrogenase |
| Reads supporting (aligned to +/- strand): ref base C (23/24); new base G (2/2); total (25/26) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CCCGTGGTGTAACCTTTTTCACGCAACGCGTTTTCCAGCACTTCCAGCGGAACACCACATTGCGCCGTCGCCTGCATATTCTCAATATCAATATTAATGATTTGATTCATGGCGGAGCCGTCGAGCACCACCGAGTT > W3110S.gb/2897653‑2897789 | cccGTGGTGTAACCTTTTTCACGCAACGCGTTTTCCAGCACCTCCAGCGGAACGCCACATTGCGCCGTGGc < 1:1161142/71‑1 (MQ=255)cccGTGGTGTAACCTTTTTCACGCAACGCGTTTTCCAGCACCTCCAGCGGAACGCCACATTGCGCCGTGGc < 1:260626/71‑1 (MQ=255) gtggtgTAACCTTTTTCACGCAACGCGTTTTCCAGCACCTCCAGCGGAACGCCACATTGCGCCGTGGCCTg > 1:222469/1‑71 (MQ=255) gtggtgTAACCTTTTTCACGCAACGCGTTTTCCAGCACCTCCAGCGGAACGCCACATTGCGCCGTGGCCTg > 1:29706/1‑71 (MQ=255) tggtgTAACCTTTTTCACGCAACGCGTTTTCCAGCACTTCCAGCGGAACACCACATTGCGCCGTCGCCTg > 1:4347363/1‑70 (MQ=255) tggtgTAACCTTTTTCACGCAACGCGTTTTCCAGCACTTCCAGCGGAACACCACATTGCGCCGTCGCCTg > 1:1728696/1‑70 (MQ=255) gtgtAACCTTTTTCACGCAACGCGTTTTCCAGCACTTCCAGCGGAACACCACATTGCGCCGTCGCCTGCa < 1:2776690/70‑1 (MQ=255) tAACCTTTTTCACGCAACGCGTTTTCCAGCACTTCCAGCGGAACACCACATTGCGCCGTCGcc > 1:3125274/1‑63 (MQ=255) aCCTTTTTCACGCAACGCGTTTTCCAGCACTTCCAGCGGAACACCACATTGCGCCGTCGCCTGCATATtct > 1:3024536/1‑71 (MQ=255) aCCTTTTTCACGCAACGCGTTTTCCAGCACTTCCAGCGGAACACCACATTGCGCCGTCGCCTGCATATtct > 1:2234318/1‑71 (MQ=255) ccTTTTTCACGCAACGCGTTTTCCAGCACTTCCAGCGGAACACCACATTGCGCCGTCGCCTGCATATtctc < 1:3429951/71‑1 (MQ=255) cTTTTTCACGCAACGCGTTTTCCAGCACTTCCAGCGGAACACCACATTGCGCCGTCGCCTg < 1:2240662/61‑1 (MQ=255) cTTTTTCACGCAACGCGTTTTCCAGCACTTCCAGCGGAACACCACATTGCGCCGTCGCCTGCATATtctc > 1:2380698/1‑70 (MQ=255) ttttCACGCAACGCGTTTTCCAGCACTTCCAGCGGAACACCACATTGCGCCGTCGCCTGCa > 1:487777/1‑61 (MQ=255) tttCACGCAACGCGTTTTCCAGCACTTCCAGCGGAACACCACATTGCGCCGTCGCCTGCatat > 1:1530306/1‑63 (MQ=255) tttCACGCAACGCGTTTTCCAGCACTTCCAGCGGAACACCACATTGCGCCGTCGCCTGCatat > 1:1122412/1‑63 (MQ=255) tCACGCAACGCGTTTTCCAGCACTTCCAGCGGAACACCACATTGCGCCGTCGCCTGCATATTCtcaatat < 1:898369/70‑1 (MQ=255) cACGCAACGCGTTTTCCAGCACTTCCAGCGGAACACCACATTGCGCCGTCGCCTGCATATTCtcaatatc > 1:2001845/1‑70 (MQ=255) cACGCAACGCGTTTTCCAGCACTTCCAGCGGAACACCACATTGCGCCGTCGCCTGCATATTCtcaatatc < 1:2701145/70‑1 (MQ=255) cAACGCGTTTTCCAGCACTTCCAGCGGAACACCACATTGCGCCGTCGCCTGCATATTCTcaatatcaatat < 1:4277807/71‑1 (MQ=255) aCGCGTTTTCCAGCACTTCCAGCGGAACACCACATTGCGCCGTCGCCTGCATATtctc < 1:811008/58‑1 (MQ=255) aCGCGTTTTCCAGCACTTCAAGCGGAACACCACATTGCGCCGTCGCCTGCATATtctc < 1:4077669/58‑1 (MQ=255) cgcgTTTTCCAGCACTTCCAGCGGAACACCACATTGCGCCGTCGCCTGCATATTCTCAATATCAATATTaa > 1:2601193/1‑71 (MQ=255) cgTTTTCCAGCACTTCCAGCGGAACACCACATTGCGCCGTCGCCTGCATATTCTCAATATCAATATTa < 1:3264145/68‑1 (MQ=255) ttttCCAGCACTTCCAGCGGAACACCACATTGCGCCGTCGCCTGCATATTCtcaatatcaat < 1:2322407/62‑1 (MQ=255) ttCCAGCACTTCCAGCGGAACACCACATTGCGCCGTCGCCTGCATATTCTCAATATCAATATTAAtgattt > 1:4326314/1‑71 (MQ=255) tCCAGCACTTCCAGCGGAACACCACATTGCGCCGTCGCCTGCATATTCTCAATATCAATATTAAtgattt < 1:2878113/70‑1 (MQ=255) tCCAGCAATTCCAGCGGAACACCACATTGCGCCGTCGCCTGCATATTCTCAATATCAATATTAAtgattt < 1:1234986/70‑1 (MQ=255) aCTTCCAGCGGAACACCACATTGCGCCGTCGCCTGCATATTCTCAATATCAATATTAATGATTTGATTCAt < 1:1797788/71‑1 (MQ=255) cTTCCAGCGGAACACCACATTGCGCCGTCGCCTGCATATTCTCAATATCAATATTAAtgatttgatt < 1:1582520/67‑1 (MQ=255) ttCCAGCGGAACACCACATTGCGCCGTCTCCTGCATATTCTCAATATCAATATTAAtgatttg < 1:2148540/63‑1 (MQ=255) tCCAGCGGAACACCACATTGCGCCGTCGCCTGCata > 1:3616281/1‑36 (MQ=255) tCCAGCGGAACACCACATTGCGCCGTCGCCTGCata > 1:3740625/1‑36 (MQ=255) ccAGCGGAACACCACATTGCGCCGTCGCCTGCATATTCTCAATATCAATATTAATGATTTGATTCATGGCg > 1:1565575/1‑71 (MQ=255) gCGGAACACCACATTGCGCCGTCGCCTGCATATTCTCAATATCAATATTAATGATTTGATTCATGGCGGAg > 1:3739125/1‑71 (MQ=255) cGGGACACCACATTGCGCCGTCGCCTGCATATTCTCAATATCAATATTAATGATTTGATTCATGGCGGAGc > 1:3360079/1‑71 (MQ=255) cGGAACACCACATTGCGCCGTCGCCTGCATATTCTCAATATCAATATTAATGATTTGATTCATGGCGGAGc > 1:1268521/1‑71 (MQ=255) acacCACATTGCGCCGTCGCCTGCATATTCtcaatatcaata > 1:3245571/1‑42 (MQ=255) ccacATTGCGCCGTCGCCTGCATATTCTCAATATCAATATTAATGATTTGATTCATGGAGGAGCCGTCGAg < 1:3839540/71‑1 (MQ=255) caTTGCGCCGTCGCCTGCATATTCTCAATATCAATATTAATGATTTGATTCATGGCGGAGCCGTCGAGcac > 1:4340274/1‑71 (MQ=255) aTTGCGCCGTCGCCTGCATATTCTCAATATCAATATTAAtgattt < 1:1072903/45‑1 (MQ=255) ttGCGCCGTCGCCTGCATATTCTCAATATCAATATTAAt < 1:261258/39‑1 (MQ=255) tGCGCCGTCGCCTGCATATTCTCAATATCAATATTAAtgatttga > 1:1816324/1‑45 (MQ=255) tGCGCCGTCGCCTGCATATTCTCAATATCAATATTAAtgatttga > 1:2231718/1‑45 (MQ=255) gcgcCGTCGCCTGCATATTCTCAATATCAATATTAAtgatttga > 1:3884331/1‑44 (MQ=255) gTCGCCTGCATATTCTCAATATCAATATTAATGATTTGATTCATGGCGGAGCCGTCGAGCACCACCGAGtt < 1:1842624/71‑1 (MQ=255) gTCGCCTGCATATTCTCAATATCAATATTAATGATTTGATTCATGGCGGAGCCGTCGAGCACCACCGAGtt < 1:732810/71‑1 (MQ=255) gTCGCCTGCATATTCTCAATATCAATATTAATGATTTGATTCATGGCGGAGCCGTCGAGCACCACCGAGtt < 1:2912072/71‑1 (MQ=255) tCGCCTGCATATTCTCAATATCAATATTAAtgatttgatt < 1:2267556/40‑1 (MQ=255) tCGCCTGCATATTCTCAATATCAATATTAAtgatttgatt < 1:1639999/40‑1 (MQ=255) tCGCCTGCATATTCTCAATATCAATATTAAtgatttg < 1:2341609/37‑1 (MQ=255) | CCCGTGGTGTAACCTTTTTCACGCAACGCGTTTTCCAGCACTTCCAGCGGAACACCACATTGCGCCGTCGCCTGCATATTCTCAATATCAATATTAATGATTTGATTCATGGCGGAGCCGTCGAGCACCACCGAGTT > W3110S.gb/2897653‑2897789 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |