| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 3,083,446 | A→C | 9.5% | S374S (TCT→TCG) | speA ← | biosynthetic arginine decarboxylase, PLP‑binding |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,083,446 | 0 | A | C | 9.5% | 136.5 / 10.3 | 63 | S374S (TCT→TCG) | speA | biosynthetic arginine decarboxylase, PLP‑binding |
| Reads supporting (aligned to +/- strand): ref base A (34/23); new base C (2/4); total (36/27) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.88e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.93e-01 | |||||||||||
CGCGCGGCGCATCTTCTGCAGGCGCGGTCGGCACCGTGTATTCGTTACGTTCCACGCCGATGATATTAGACACCAGCACGGTGTGATGCGCAGTCACCGCACGACCCGATTCGGTGATTACCGTCGGATGCGGCAGAC > W3110S.gb/3083379‑3083516 | cgcgcgGCGCATCTTCTGCAGGCGCGGTCGGCACCGTGTATTCGTTACGTTCCACGCCGATGATATTAGac < 1:282044/71‑1 (MQ=255) gcggcgCATCTTCTGCAGGCGCCGTCGGCACCGTGTATTCGTTACGTTCCACGCCGATGATATTAGacac > 1:3271711/1‑70 (MQ=255) cgcATCTTCTGCAGGCGCGGTCGGCACCGTGTATTCGTTACGTTCCACGCCGATGATATTAGa > 1:1529331/1‑63 (MQ=255) cATCTTCTGCAGGCGCGGTCGGCACCGTGTATTCGTTACGTTCCACGCCGATGATATTAGACACCAGCACg < 1:27908/71‑1 (MQ=255) cATCTTCTGCAGGCGCGGTCGGCACCGTGTATTCGTTACGTTCCACGCCGATGATATTAGACACCAGCACg < 1:206175/71‑1 (MQ=255) tcttctGCAGGCGCGGTCGGCACCGTGTATTCGTTACGTTCCACGCCGATGATATTAGACACCAGc < 1:381354/66‑1 (MQ=255) tcttctGCAGGCGCGGTCGGCACCGTGTATTCGTTACGTTCCACGCCGATGATATTAGACACCAGCACGgt > 1:3117575/1‑71 (MQ=255) cttctGCAGGCGCGGTCGGCACCGTGTATTCGTTACGTTCCACGCCGATGATATTa < 1:656337/56‑1 (MQ=255) ttctGCAGGCGCGGTCGGCACCGTGTATTCGTTACGTTCCACGCCGATTATATTAGACACCAGCa > 1:3814953/1‑65 (MQ=255) ttctGCAGGCGCGGTCGGCACCGTGTATTCGTTACGTTCCACGCCGATGATATTAGACACCAGCa > 1:2859409/1‑65 (MQ=255) ttctGCAGGCGCGGTCGGCACCGTGTATTCGTTACGTTCCACGCCGATGATATTAGACACCAGCACGgtg > 1:496077/1‑70 (MQ=255) ttctGCAGGCGCGGTCGGCACCGTGTATTCGTTACGTTCCACGCCGATGATATTAGACACCAGCACGgt > 1:3436970/1‑69 (MQ=255) ctGCAGGCGCGGTCGGCACCGTGTATTCGTTACGTTCCACGCCGATGATATTCGACACCAGCACGGtgtg < 1:3349010/70‑1 (MQ=255) ctGCAGGCGCGGTCGGCACCGTGTATTCGTTACGTTCCACGCCGATGATATTCGACACCAGCACGGtgtg < 1:3562072/70‑1 (MQ=255) ctGCAGGCGCGGTCGGCACCGTGTATTCGTTACGTTCCACGCCGATGATATTCGACACCAGCACGGtgtg < 1:2382545/70‑1 (MQ=255) ctGCAGGCGCGGTCGGCACCGTGTATTCGTTACGTTCCACGCCGATGATATTCGACACCAGCACGGtgtg < 1:1114918/70‑1 (MQ=255) ctGCAGGCGCGGTCGGCACCGTGTATTCGTTACGTTCCACGCCGATGATATTAGACACCAGCACGgtgt < 1:577555/69‑1 (MQ=255) ctGCAGGCGCGGTCGGCACCGTGTATTCGTTACGTTCCACGCCGATGATATTAGACACCAGCACGgtgt < 1:2288871/69‑1 (MQ=255) ctGCAGGCGCGGTCGGCACCGTGTATTCGTTACGTTCCACGCCGATGATATTAGACACCAGCACGgtgt < 1:2753115/69‑1 (MQ=255) tGCAGGCGCTGTCGGCACCGTGTATTCGTTACGTTCCACGCCGATGATATTAGACACCAGCACGgtgt < 1:643528/68‑1 (MQ=255) aGGCGCGGTCGGCACCGTGTATTCGTTACGTTCCACGCCGATGATATTAGACACCAGCACGGTGTGATgcg > 1:8293/1‑71 (MQ=255) gcgcGGTCGGCACCGTGTATTCGTTACGTTCCACGCCGATGATATTAGACACCAGCACGGTGTGATGCGCa < 1:2297116/71‑1 (MQ=255) cgcgGTCGGCACCGTGTATTCGTTACGTTCCACGCCGATGATATTAGACACCAGCACGGTGTGa > 1:4239764/1‑64 (MQ=255) cgGTCGGCACCGTGTATTCGTTACGTTCCACGCCGATGATATTAGACACCAGCACGGTGTGATGCGCAGTc < 1:2771338/71‑1 (MQ=255) ggTCGGCACCGTGTATTCGTTACGTTCCACGCCGATGATATTAGACACCAGCACGGTGTGATGCGCAGTc < 1:3734350/70‑1 (MQ=255) gTCGGCACCGTGTATTCGTTACGTTCCACGCCGATGATATTAGACACCAGCACTgtgt < 1:396441/58‑1 (MQ=255) gTCGGCACCGTGTATTCGTTACGTTCCACGCCGATGATATTAGACACCAGCACGgtgt < 1:1654847/58‑1 (MQ=255) tCGGCACCGTGTATTCGTTACGTTCCACGCCGATGATATTAGACACCAGc < 1:2336622/50‑1 (MQ=255) ggCACCGTGTATTCGTTACGTTCCACGCCGATGATATTAGACACCAGCACGgtgt < 1:626645/55‑1 (MQ=255) ggCACCGTGTATTCGTTACGTTCCACGCCGATGATATTAGACACCAGCACGGTGTGATGCGCAGTCACCg < 1:1984563/70‑1 (MQ=255) ccGTGTATTCGTTACGTTCCACGCCGATGATATTCGACACCAGCACGGTGTGATGCGCAGTCACCGCACGa > 1:526845/1‑71 (MQ=255) ccGTGTATTCGTTACGTTCCACGCCGATGATATTCGACACCAGCACGGTGTGATGCGCAGTCACCGCACGa > 1:4055186/1‑71 (MQ=255) ccGTGTATTCGTTACGTTCCACGCCGATGATATTAGACACCAGCACGGTGTGATGCGCAGTCACCGCACGa > 1:3561437/1‑71 (MQ=255) ccGTGTATTCGTTACGTTCCACGCCGATGATATTAGACACCAGCACGGTGTGATGCGCAGTCACCGCACGa > 1:2073153/1‑71 (MQ=255) ccGTGTATTCGTTACGTTCCACGCCGATGATATTAGACACCAGCACGGTGTGATGCGCAGTCACCGCACGa > 1:3520160/1‑71 (MQ=255) tgtATTCGTTACGTTCCACGCCGATGATATTAGACACCAGCACGGTGTGATGCGCAGTCACCGCACGAccc > 1:3666581/1‑71 (MQ=255) tgtATTCGTTACGTTCCACGCCGATGATATTAGACACCAGCACGGTGTGATGCGCAGTCACCGCACGAccc > 1:2387079/1‑71 (MQ=255) gtATTCGTTACGTTCCACGCCGATGATATTAGACACCAGCACGGTGTGATGCGCAGTCACCGCACGAccc > 1:2099640/1‑70 (MQ=255) tATTCGTTACGTTCCACGCCGATGATATTAGACACCAGCACGgtgt < 1:2543363/46‑1 (MQ=255) aTTCGTTACGTTCCACGCCGATGATATTAGACACCAGCACGGTGTGATGCGCAGTCACCGCACGACCCGAt > 1:2736061/1‑71 (MQ=255) ttCGTTACGTTCCACGCCGATGATATTAGACACCAGCACGGTGTGa > 1:206153/1‑46 (MQ=255) ttCGTTACGTTCCACGCCGATGATATTAGACACCAGCACGGTGTGa > 1:4356686/1‑46 (MQ=255) ttCGTTACGTTCCACGCCGATGATATTAGACACCAGCACGGTGTGa > 1:1433170/1‑46 (MQ=255) cGTTACGTTCCACGCCGATGATATTAGACACCAGCACGGTGTGATGCGCAGTCACCGCACGACCCGATTc < 1:2840669/70‑1 (MQ=255) cGTTACGTTCCACGCCGATGATATTAGACACCAGCACGGTGTGATGCGCAGTCACCGCACGACCCGATTc < 1:965987/70‑1 (MQ=255) aCGTTCCACGCCGATGATATTAGACACCAGCACgg > 1:3549231/1‑35 (MQ=255) aCGTTCCACGCCGATGATATTAGACACCAGCACGGTGTGa > 1:2337158/1‑40 (MQ=255) aCGTTCCACGCCGATGATATTAGACACCAGCACGGTGTGATGCGCAGTCACCGCACGACCCGATTCGGTGa > 1:3517773/1‑71 (MQ=255) cGTTCCACGCCGATGATATTAGACACCAGCACGGTGTGATGCGCAGTCACCGCACGACCCGATTCGGTGAt > 1:3707138/1‑71 (MQ=255) cGTTCCACGCCGATGATATTAGACACCAGCACGGTGTGATGCGCAGTCACCGCACGACCCGATTCGGTGAt > 1:811768/1‑71 (MQ=255) gTTCCACGCCGATGATATTAGACACCAGCACGgtgt < 1:1150339/36‑1 (MQ=255) gTTCCACGCCGATGATATTAGACACCAGCACGGTGTGATGCGCAGTCACCGCACGACCCGATTCGGTGAtt < 1:1244221/71‑1 (MQ=255) ttCCACGCCGATGATATTAGACACCAGCACGgtgt < 1:1794800/35‑1 (MQ=255) tCCACGCCGATGATATTAGACACCAGCACGGTGTGATGCGCAGTCAc > 1:1493737/1‑47 (MQ=255) ccACGCCGATGATATTAGACACCAGCACGGTGTGATGCGCAGTCAc > 1:4099832/1‑46 (MQ=255) ccGATCATATTAGACACCAGCACGGTGTGATGCCCAGTCACCGCACGACCCGATTCGGTGATTACCGTCg > 1:1284712/1‑70 (MQ=255) gatgatATTAGACACCAGCACGGTGTGATGCGCAGTCACCGCACGACCCGATTCGGTGATTACCGTCGGAt < 1:4276332/71‑1 (MQ=255) tgatATTAGACACCAGCACGGTGTGATGCGCAGTCACCGCACGACCCGATTCGGTGATTAc > 1:1374946/1‑61 (MQ=255) gatATTAGACACCAGCACGGTGTGATGCGCAGTCACCGCACGACCCGATTCGGTGATTACCGTCGGATGCg > 1:2904834/1‑71 (MQ=255) tatTAGACACCAGCACGGTGTGATGCGCAGTCACCGCACGACCCGATTCGGTGATTACCGTCGGATGCgg > 1:2741596/1‑70 (MQ=255) atTAGACACCAGCACGGTGTGATGCGCAGTCACCGCACGACCCGATTCGGTGATTACCGTCGGATGCgg > 1:1258808/1‑69 (MQ=255) ttAGACACCAGCACGGTGTGATGCGCAGTCACCGCACGa > 1:3551131/1‑39 (MQ=255) ttAGACACCAGCACGGTGTGATGCGCAGTCACCGCACGa > 1:3068891/1‑39 (MQ=255) ttAGACACCAGCACGGTGTGATGCGCAGTCACCGCACGACCCGATTCGGTGATAACCGTCGGATGCgg > 1:830390/1‑68 (MQ=255) aGACACCAGCACGGTGTGATGCGCAGTCACCGCACGACCCGATTCGGTGATTACCGTCGGATGCGGCAGAc > 1:2441613/1‑71 (MQ=255) aGACACCAGCACGGTGTGATGCGCAGTCACCGCACGACCCGATTCGGTGATTACCGTCGGATGCGGCAGAc > 1:1396134/1‑71 (MQ=255) | CGCGCGGCGCATCTTCTGCAGGCGCGGTCGGCACCGTGTATTCGTTACGTTCCACGCCGATGATATTAGACACCAGCACGGTGTGATGCGCAGTCACCGCACGACCCGATTCGGTGATTACCGTCGGATGCGGCAGAC > W3110S.gb/3083379‑3083516 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |