| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 3,089,635 | C→T | 6.1% | R211R (CGC→CGT) | endA → | DNA‑specific endonuclease I |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,089,635 | 0 | C | T | 6.1% | 200.0 / 2.2 | 67 | R211R (CGC→CGT) | endA | DNA‑specific endonuclease I |
| Reads supporting (aligned to +/- strand): ref base C (32/30); new base T (2/2); total (35/32) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.09e-01 | |||||||||||
GCCAGCAAACGCAGCTGTTCAACGCATGGAACAAGATGTATCCGGTTACCGACTGGGAGTGCGAGCGCGATGAACGCATCGCGAAGGTGCAGGGCAATCATAACCCGTATGTGCAACGCGCTTGCCAGGCGCGAAA > W3110S.gb/3089568‑3089703 | gCCAGCAAACGCAGCTGTTCAACGCATGGAACAAGATGTATCCGGTTACCGACTGGGAGTGCGAgcgc < 1:1113266/68‑1 (MQ=255) cAGCAAACGCAGCTGTTCAACGCATGGAACAAGATGTATCCGGTTACCGACTGGGAGTGCGAGCGCGATg > 1:321727/1‑70 (MQ=255) cAGCAAACGCAGCTGTTCAACGCATGGAACAAGATGTATCCGGTTACCGACTGGGAGTGCGAGCGCGATGa < 1:168276/71‑1 (MQ=255) cAGCAAACGCAGCTGTTCAACGCATGGAACAAGATGTATCCGGTTACCGACTGGGAGTGCGAGCGCGATGa < 1:1570655/71‑1 (MQ=255) gCAAACGCAGCTGTTCAACGCATGGAACAAGATGTATCCGGTTACCGACTGGGAGTGCGAGCGCGATg < 1:405594/68‑1 (MQ=255) aaaCGCAGCTGTTCAACGCATGGGACAAGATGTATCCAGTTACCGACTGGGAGTGCGAGCGTGATGAACGc < 1:1046204/71‑1 (MQ=255) aaaCGCAGCTGTTCAACGCATGGGACAAGATGTATCCAGTTACCGACTGGGAGTGCGAGCGTGATGAACGc < 1:996372/71‑1 (MQ=255) aaaCGCAGCTGTTCAACGCATGGAACAAGATGTATCCGGTTACCGACTGGGAGTGCGAGCGCGATGAACGc < 1:3480487/71‑1 (MQ=255) aCGCAGCTGTTCAACGCATGGAACAAGATGTATCCGGTTACCGACTGGGAGTGCGAGCGCGATGAACGCAt > 1:1655161/1‑71 (MQ=255) aCGCAGCTGTTCAACGCATGGAACAAGATGTATCCGGTTACCGACTGGGAGTGCGAGCGCGATGAACGCAt > 1:3535114/1‑71 (MQ=255) aCGCAGCTGTTCAACGCATGGAACAAGATGTATCCGGTTACCGACTGGGAGTGCGAGCGCGATGAACGCAt < 1:3899941/71‑1 (MQ=255) aCGCAGCTGTTCAACGCATGGAACAAGATGTATCCGGTTACCGACTGGGAGTGCGAGCGCGATGAACGCAt < 1:1222595/71‑1 (MQ=255) cGCAGCTGTTCAACGCATGGAACAAGATGTATCCGGTTACCGACTGGGAGTGCGAGCGCGATGAACGCATc > 1:1012442/1‑71 (MQ=255) cGCAGCTGTTCAACGCATGGAACAAGATGTATCCGGTTACCGACTGGGAGTGCGAGCGCGATGAACGCATc > 1:972432/1‑71 (MQ=255) cGCAGCTGTTCAACGCATGGAACAAGATGTATCCGGTTACCGACTGGGAGTGCGAGCGCGATGAACGCATc > 1:2638998/1‑71 (MQ=255) aGCTGTTCAACGCATGGAACAAGATGTATCCGGTTACCGACTGGGAGTGCGAGCGCGa > 1:1590075/1‑58 (MQ=255) gCTGTTCAACGCATGGAACAAGATGTATCCGGTTACCGACTGGGAGTGCGAGCGCGATGAACGCATCGCGa < 1:1315767/71‑1 (MQ=255) gCTGTTCAACGCATGGAACAAGATGTATCCGGTTACCGACTGGGAGTGCGAGCGCGATGAACGCATCGCGa < 1:2056088/71‑1 (MQ=255) gCTGTTCAACGCATGGAACAAGATGTATCCGGTTACCGACTGGGAGTGCGAGCGCGATGAACGCATCGCGa < 1:1833156/71‑1 (MQ=255) cTGTTCAACGCATGGAACAAGATGTATCCGGTTACCGACTGGGAGTGCGAGCGCGATGAACGCATcgcg > 1:1867452/1‑69 (MQ=255) cTGTTCAACGCATGGAACAAGATGTATCCGGTTACCGACTGGGAGTGCGAGCGCGATGAACGCATCGCGaa < 1:3292544/71‑1 (MQ=255) gTTCAACGCATGGAACAAGATGTATCCGGTTACCGACTGGGAGTGCGAGCGCGATGAACGCATCGCGa < 1:4378178/68‑1 (MQ=255) ttCAACGCATGGAACAAGATGTATCCGGTTACCGACTGGGAGTGCGAGCGCGATGAACGCATCGCGAAGGt > 1:521523/1‑71 (MQ=255) tCAACGCATGGAACAAGATGTATCCGGTTACCGACTGGGAGTGCGAGCGCGATGAACGCATCGCGAAg > 1:2395708/1‑68 (MQ=255) aCGCATGGAACAAGATTTATCCGGTTACCGACTGGGAGTGCGAGCGCGATGAACGCATCGCGAAGGTGCAg > 1:4212739/1‑71 (MQ=255) tGGAACAAGATGTATCCGGTTACCGACTGGGAGTGCGAGCGCGATGAACGCATCGCGAAGGTGCAGGGCaa < 1:33496/71‑1 (MQ=255) ggAACAAGATGTATCCGGTTACCGACTGGGAGTGCGAGCGCGATGAACGCATCGCGa < 1:1664489/57‑1 (MQ=255) gAACAAGATGTATCCGGTTACCGACTGGGAGTGCGAGCGGGATGAACGCATCGCGaa > 1:330872/1‑57 (MQ=255) gAACAAGATGTATCCGGTTACCGACTGGGAGTGCGAGCGCGATGAACGCAt > 1:1633014/1‑51 (MQ=255) gAACAAGATGTATCCGGTTACCGACTGGGAGTGCGAGCGCGATGAACGCAt > 1:2883269/1‑51 (MQ=255) gAACAAGATGTATCCGGTTACCGACTGGGAGTGCGAGCGCGATGAACGCATCGCGAAGGTGc > 1:1759892/1‑62 (MQ=255) gAACAAGATGTATCCGGTTACCGACTGGGAGTGCGAGCGCGATGAACGCATCGCGAAGGTGCAGGGCAAt > 1:4334473/1‑70 (MQ=255) gAACAAGATGTATCCGGTTACCGACTGGGAGTGCGAGCGCGATGAACGCATCGCGAAGGTGCAGGGCAAt > 1:4183224/1‑70 (MQ=255) gAACAAGATGTATCCGGTTACCGACTGGGAGTGCGAGCGCGATGAACGCATCGCGAAGGTGCAGGGCAAt > 1:3291917/1‑70 (MQ=255) gAACAAGATGTATCCGGTTACCGACTGGGAGTGCGAGCGCGATGAACGCATCGCGAAGGTGCAGGGCAAt > 1:1596620/1‑70 (MQ=255) gAACAAGATGTATCCGGTTACCGACTGGGAGTGCGAGCGCGATGAACGCATCGCGAAGGTGCAGGGCAAt > 1:2091776/1‑70 (MQ=255) aaCAAGATGTATCCGGTTACCGACTGGGAGTGCGAGCGCGATGAACGCATCGCGa < 1:3199405/55‑1 (MQ=255) aaCAAGATGTATCCGGTTACCGACTGGGAGTGCGAGCGCGATGAACGCATCGCGAAGGTGCAGGGCAATc > 1:824161/1‑70 (MQ=255) aCAAGATGTATCCGGTTACCGACTGGGAGTGCGAGCGCGATGAACGCATCGCGAAGGTGCAGGGCAATCAt < 1:3423078/71‑1 (MQ=255) cAAGATGTATCCAGTTACCGACTTGGAGTGCGAGCGTGATGAACGCATCGCGAAGGTGCAGGGCAATCAt > 1:3026408/1‑70 (MQ=255) cAAGATGTATCCAGTTACCGACTTGGAGTGCGAGCGTGATGAACGCATCGCGAAGGTGCAGGGCAATCAt > 1:3021518/1‑70 (MQ=255) aGATGTATCCGGTTACCGACTGGGAGTGCGAGCGCGATGAACGCATCGCGAAGGTGCAGGGCAATCAt > 1:4019202/1‑68 (MQ=255) gTATCCGGTTACCGACTGGGAGTGCGAGCGCGATGAACGCATCGCGAAGGTGCAGGGCAATCATAACCCg > 1:2633123/1‑70 (MQ=255) ccGGTTACCGACTGGGAGTGCGAGCGCGATGAACGCATCGCGAAGGTGCAGGGCAAt > 1:2214662/1‑57 (MQ=255) cGGTTACCGACTGGGAGTGCGAGCGCGATGAACGCATCGCGAAGGTGCAGGGCAATCATaa > 1:2785532/1‑61 (MQ=255) cGGTTACCGACTGGGAGTGCGAGCGCGATGAACGCATCGCGAAGGTGCAGGGCAATCATAACCCGTAtgtg < 1:347980/71‑1 (MQ=255) ggTTACCGACTGGGAGTGCGAGCGCGATGAACGCATCGCGAAGGTGCAGGGCAATCATAACCCCTATGTGc < 1:472272/71‑1 (MQ=255) aCTGGGAGTGCGAGCGCGATGAACGCATCGCGAAGGTg > 1:875252/1‑38 (MQ=255) aCTGGGAGTGCGAGCGCGATGAACGCATCGCGAAGGTGCAGGGCAATCATa > 1:2441117/1‑51 (MQ=255) aCTGGGAGTGCGAGCGCGATGAACGCATCGCGAAGGTGCAGGGCAATCATAACCCGTATGTGCAACGCGCt > 1:1506353/1‑71 (MQ=255) ggAGTGCGAGCGCGATGAACGCATCGCGAAGGTGCAGGGCAATCATAACCCGTATGTGCAACGCGCTTg < 1:3847276/69‑1 (MQ=255) ggAGTGCGAGCGCGATGAACGCATCGCGAAGGTGCAGGGCAATCATAACCCGTATGTGCAACGCGCTTGcc < 1:2874837/71‑1 (MQ=255) ggAGTGCGAGCGCGATGAACGCATCGCGAAGGTGCAGGGCAATCATAACCCGTATGTGCAACGCGCTTGc < 1:1532399/70‑1 (MQ=255) aGTGCGAGCGCGATGAACGCATCGCGAAGGTGCAGGGCAATCATAACCCGTATGTGCAACGCGCTTGCCAg < 1:4337556/71‑1 (MQ=255) gTGCGAGCGCGATGAACGCATCGCGAAGGTGCAGGGCAATCATAACCCGTATGTGCAACGCGCTTGCCAgg > 1:3324654/1‑71 (MQ=255) tGCGAGCGCGATGAACGCATCGCGAAGGTGCAGGGCAATCATAACCCGTAt < 1:4236902/51‑1 (MQ=255) tGCGAGCGCGATGAACGCATCGCGAAGGTGCAGGGCAATCATAACCCGTATGTGCAACGCGCTTGCCAGgc > 1:1835464/1‑71 (MQ=255) tGCGAGCGCGATGAACGCATCGCGAAGGTGCAGGGCAATCATAACCCGTATGTGCAACGCGCTTGCCAGgc > 1:1328253/1‑71 (MQ=255) gCGAGCGCGATGAACGCATCGCGAAGGTGCAGGGCAATCATAACCCGTAtgt > 1:3375619/1‑52 (MQ=255) cGAGCGCGATGAACGCATCGCGAAGGTGCAGGGCAATCATAACCCGTATGTGCAACGCGCTTGCCAGgcgc > 1:4077065/1‑71 (MQ=255) aGCGCGATGAACGCATCGCGAAGGTGCAGGGCAATCATAACCCGTATGTGCAACGCGCTTGCCAGGCGCGa < 1:2925094/71‑1 (MQ=255) aGCGCGATGAACGCATCGCGAAGGTGCAGGGCAATCATAACCCGTATGTGCAACGCGCTTGCCAGGCGCGa < 1:860934/71‑1 (MQ=255) aGCGCGATGAACGCATCGCGAAGGTGCAGGGCAATCATAACCCGTATGTGCAACGCGCTTGCCAGGCGCGa < 1:855980/71‑1 (MQ=255) aGCGCGATGAACGCATCGCGAAGGTGCAGGGCAATCATAACCCGTATGTGCAACGCGCTTGCCAGGCGCGa < 1:2672927/71‑1 (MQ=255) aGCGCGATGAACGCATCGCGAAGGTGCAGGGCAATCATAACCCGTATGTGCAACGCGCTTGCCAGGCGCGa < 1:3499591/71‑1 (MQ=255) aGCGCGATGAACGCATCGCGAAGGTGCAGGGCAATCATAACCCGTATGTGCAACGCGCTTGCCAGGCGCGa < 1:402024/71‑1 (MQ=255) aGCGCGATGAACGCATCGCGAAGGTGCAGGGCAATCATAACCCGTATGTGCAACGCGCTTGCCAGGCGCGa < 1:2577950/71‑1 (MQ=255) aGCGCGATGAACGCATCGCGAAGGTGCAGGGCAATCATAACCCGTATGTG‑GACGCGCTTGCCAGGCGCGaa < 1:4336169/71‑1 (MQ=255) gcgcGATGAACGCATCGCGAAGGTGCAGGGCAATCATAACCCGTATGTGCAACGCGCTTGCCAGGCGCGa > 1:1717738/1‑70 (MQ=255) cgcgATGAACGCATCGCGAAGGTGCAGGGCAATCATAACCCGTATGTGCAACGCGCTTGCCAGGCGCGaaa < 1:243929/71‑1 (MQ=255) | GCCAGCAAACGCAGCTGTTCAACGCATGGAACAAGATGTATCCGGTTACCGACTGGGAGTGCGAGCGCGATGAACGCATCGCGAAGGTGCAGGGCAATCATAACCCGTATGTGCAACGCGCTTGCCAGGCGCGAAA > W3110S.gb/3089568‑3089703 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |