| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 3,138,489 | T→C | 6.1% | L44L (CTA→CTG) | hybG ← | hydrogenase 2 accessory protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,138,489 | 0 | T | C | 6.1% | 264.7 / 7.3 | 98 | L44L (CTA→CTG) | hybG | hydrogenase 2 accessory protein |
| Reads supporting (aligned to +/- strand): ref base T (41/51); new base C (3/3); total (44/54) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.88e-01 | |||||||||||
GCTTTGGCTTCATCTTCGTCGATGATGCTCATGGCAAATCCGACGTGTACCAGCACCCACTGGCCCAGTAGATCGGCAGGGTTACCTTCACAAATCAGGGCGATATTCACATCGCGCTTGATACCACATACTTCAACC > W3110S.gb/3138421‑3138558 | gctttggcttCATCTTCGTCGATGATGCTCATGGCAAATCCGACGTGTACCAGCACCCACTGGCCCAGTAg < 1:860066/71‑1 (MQ=255) ggcttCATCTTCGTCGATGATGCTCATGGCAAATCCGACGTGTACCAGCACCCACTGGCCCAGTAGATCgg > 1:2353541/1‑71 (MQ=255) ggcttCATCTTCGTCGATGATGCTCATGGCAAATCCGACGTGTACCAGCACCCACTGGCCCAGTAGATCg < 1:2857780/70‑1 (MQ=255) gcttCATCTTCGTCGATGATGCTCATGGCAAATCCGACGTGTACCAGCACCCACTGGCCCAGTAGATCgg > 1:3641279/1‑70 (MQ=255) gcttCATCTTCGTCGATGATGCTCATGGCAAATCCGACGTGTACCAGCACCCACTGGCCCAGTAGATCgg < 1:3983981/70‑1 (MQ=255) cATCTTCGTCGATGATGCTCATGGCAAATCCGACGTGTACCAGCACCCACTGGCCCAGTAGATCGGCAgg < 1:2212757/70‑1 (MQ=255) cATCTTCGTCGATGATGCTCATGGCAAATCCGACGTGTACCAGCACCCACTGGCCCAGTAGATCGGCAg < 1:1652059/69‑1 (MQ=255) tCTTCGTCGATGATGCTCATGGCAAATCCGACGTGTACCAGCACCCACTGGCCCAGTAGATCGGCAGGGt > 1:4105579/1‑70 (MQ=255) tCTTCGTCGATGATGCTCATCGCAAATCCGACGTGTACAAGCACCCACTGGCCCAGt > 1:424443/1‑57 (MQ=37) tCTTCATCGATAATGCTCATGGCAAATCCGACGTGTACCAGTACCCACTGGCCCAGCAGATCGGCAGGGtt < 1:467036/71‑1 (MQ=21) tCTTCATCGATAATGCTCATGGCAAATCCGACGTGTACCAGTACCCACTGGCCCAGCAGATCGGCAGGGtt < 1:1355092/71‑1 (MQ=21) tCTTCATCGATAATGCTCATGGCAAATCCGACGTGTACCAGTACCCACTGGCCCAGCAGATCGGCAGGGtt < 1:4308966/71‑1 (MQ=21) cTTCGTCGATGATGCTCATGGCAAATCCGACGTGTACCAGCACCCACTGGCCCAGTAGATCGGCAGGGTTa > 1:497094/1‑71 (MQ=255) cTTCGTCGATGATGCTCATGGCAAATCCGACGTGTACCAGCACCCACTGGCCCAGTAGATCGGCAGGGTTa > 1:1599728/1‑71 (MQ=255) cTTCGTCGATGATGCTCATGGCAAATCAGACGTGTACCAGCACCCACTGGCCCAGTAGATCGGCAGGGTTa > 1:242822/1‑71 (MQ=255) ttCGTCGATGATGCTCATGGCAAATCCGACGTGTACCAGCACCCACTGGCCCAGTAGATCGGCAGGGTTAc > 1:478254/1‑71 (MQ=255) tcgtcgATGATGCTCATGGCAAATCCGACGTGTACCAGCACCCACTGGCCCAGTAGATCGGCAGGGTTAcc > 1:2239612/1‑71 (MQ=255) cgtcgATGATGCTCATGGCAAATCCGACGTGTACCAGCACCCACTGGCCCAGTa > 1:1744304/1‑54 (MQ=255) cgtcgATGATGCTCATGGCAAATCCGACGTGTACCAGCACCCACTGGCCCAGTAg < 1:3739337/55‑1 (MQ=255) cgtcgATGATGCTCATGGCAAATCCGACGTGTACCAGCACCCACTGGCCCAGTAGATCGGCAGGGtt < 1:1178168/67‑1 (MQ=255) cgtcgATGATGCTCATGGCAAATCCGACGTGTACCAGCACCCACTGGCCCAGTAGATCGGCAGGGTTAcc < 1:1223302/70‑1 (MQ=255) cgtcgATGATGCTCATGGCAAATCCGACGTGTACCAGCACCCACTGGCCCAGTAGATCGGCAGGGTTAcc > 1:3122335/1‑70 (MQ=255) cgtcgATGATGCTCATGGCAAATCCGACGTGTACCAGCACCCACTGGCCCAGTAGATCGGCAGGGTTACCt > 1:1947101/1‑71 (MQ=255) cgtcgATGATGCTCATGGCAAATCCGACGTGTACCAGCACCCACTGGCCCAGTAGATCGGCAGGGTTACCt > 1:708247/1‑71 (MQ=255) tcgATGATGCTCATGGCAAATCCGACGTGTACCAGCACCCACTGGCCCAGTAGATCGGCAGGGTTAcc > 1:1389057/1‑68 (MQ=255) tcgATGATGCTCATGGCAAATCCGACGTGTACCAGCACCCACTGGCCCAGTAGATCGGCAGGGTTACCtt < 1:3119613/70‑1 (MQ=255) gatgatGCTCATGGCAAATCCGACGTGTACCAGCACCCACTGGCCCAGTAGATCGGCAGGGTTACCTTca > 1:3249373/1‑70 (MQ=255) gatgatGCTCATGGCAAATCCGACGTGTACCAGCACCCACTGGCCCAGTAGATCGGCAGGGTTACCTTca > 1:803374/1‑70 (MQ=255) atgatgCTCATGGCAAATCCGACGTGTACCAGCACCCACTGGCCCAGTAGATCGGCAGGGTTACCTTcaca < 1:1583976/71‑1 (MQ=255) tgatgCTCATGGCAAATCCGACGTGTACCAGCACCCACTGGCCCAATAGATCGGCAGGGTTACCTTCACaa < 1:1907852/71‑1 (MQ=255) atgCTCATGGCAAATCCGACGTGTACCAGCACCCACTGGCCCAGTAGATCGGCAGGGTTAc > 1:3910619/1‑61 (MQ=255) atgCTCATGGCAAATCCGACGTGTACCAGCACCCACTGGCCCAGTAGATCGGCAGGGTTAc > 1:3752090/1‑61 (MQ=255) atgCTCATGGCAAATCCGACGTGTACCAGCACCCACTGGCCCAGTAGATCGGCAGGGTTACCTTCACAAAt < 1:198163/71‑1 (MQ=255) tgCTCATGGCAAATCCGACGTGTACCAGCACCCACTGGCCCAGTAGATCGGCAGGGTTACCTTCACAAATc < 1:3787935/71‑1 (MQ=255) cTCATGGCAAATCCGACGTGTACCAGCACCCACTGGCCCAGTAGATCGGCAGGGTTACCTTCACAAATCa < 1:2113654/70‑1 (MQ=255) tCATGGCAAATCCGACGTGTACCAGCACCCACTGGCCCAGTAGATCGGCAGGGTTACCTTCACAAAt < 1:2025544/67‑1 (MQ=255) tCATGGCAAATCCGACGTGTACCAGCACCCACTGGCCCAGTAGATCGGCAGGGTTACCTTCACAAATCAgg > 1:748226/1‑71 (MQ=255) cATGGCAAATCCGACGTGTACCAGCACCCACTGGCCCAGTAGATCGGCAGGGTTACCTTCACAAATCAggg > 1:677695/1‑71 (MQ=255) tGGCAAATCCGACGTGTACCAGCACCCACTGGCCCAGTAGATCGGCAGGGTTACCTTCACAAATCAGGGc > 1:1497174/1‑70 (MQ=255) gCAAATCCGACGTGTACCAGCACCCACTGGCCCAGTAGATCGGCAGGGtt > 1:3091837/1‑50 (MQ=255) gCAAATCCGACGTGTACCAGCACCCACTGGCCCAGTAGATCGGCAGGGTTACCTTCACAAATCAGGGCGa > 1:2852832/1‑70 (MQ=255) gCAAATCCGACGTGTACCAGCACCCACTGGCCCAGTAGATCGGCAGGGTTACCTTCACAAATCAGGGCGa > 1:1487199/1‑70 (MQ=255) cAAATCCGACGTGTACCAGCACCCACTGGCCCAGTAGATCGGCAGGGTTACCTTcaca > 1:3238767/1‑58 (MQ=255) cAAATCCGACGTGTACCAGCACCCACTGGCCCAGTAGATCGGCAGGGTTACCTTCACAAATCAGGGCGa < 1:1631482/69‑1 (MQ=255) aaTCCGACGTGTACCAGCACCCACTGGCCCAGTAGATCGGCAGGGTTACCTTCACAAATCAGGGCGATAtt < 1:536129/71‑1 (MQ=255) tCCGACGTGTACCAGCACCCACTGGCCCAGTAGATCGGCAGGGtt > 1:2379263/1‑45 (MQ=255) tCCGACGTGTACCAGCACCCACTGGCCCAGTAGATCGGCAGGGTTACCTTCACAAATCAGGGCGATATTca > 1:100895/1‑71 (MQ=255) ccGACGTGTACCAGCACCCACTGGCCCAGTAGATCGGCAGGGTTACCTTCACAAATCAGGGCGATATTca > 1:4279016/1‑70 (MQ=255) aCGTGTACCAGCACCCACTGGCCCAGTAGATCGGCAGGGTTACCTTCACAAATCAGGGCGATATTCACATc > 1:1027541/1‑71 (MQ=255) cGTGTACCAGCACCCACTGGCCCAGTAGATCGGCAGGGTTACCTTCACAAATCAGGGCGATATTCACATcg < 1:1847214/71‑1 (MQ=255) gtgtACCAGCACCCACTGGCCCAGTAGATCGGCAGGGTTACCTTCACAAATCAGGGCGATATTCACATcgc < 1:810628/71‑1 (MQ=255) gtACCAGCACCCACTGGCCCAGTAGATCGGCAGGGTTACCTTCACAAATCAg > 1:636083/1‑52 (MQ=255) gtACAAGCACCCACTGGCCCAGTAGATCGGCAGGGTTACCt < 1:1926305/41‑1 (MQ=255) aCCAGCACCCACTGGCCCAGTAGATCGGCAGGGTTACCTTCACAAATCAGGGCGATATTCACATCGCGCtt > 1:2979917/1‑71 (MQ=255) ccAGCACCCACTGGCCCAGTAGATCGGCAGGGTTACCTTcaca < 1:1341938/43‑1 (MQ=255) ccAGCACCCACTGGCCCAGTAGATCGGCAGGGTTACCTTcaca < 1:1956119/43‑1 (MQ=255) ccAGCACCCACTGGCCCAGTAGATCGGCAGGGTTACCTTCACAAATCa > 1:4141507/1‑48 (MQ=255) ccAGCACCCACTGGCCCAGTAGATCGGCAGGGTTACCTTCACAAATCAGGGCGATATTCACATCGCGCtt < 1:3760229/70‑1 (MQ=255) ccAGCACCCACTGGCCCAGTAGATCGGCAGGGTTACCTTCACAAATCAGGGCGATATTCACATCGCGCtt < 1:947229/70‑1 (MQ=255) ccAGCACCCACTGGCCCAGTAGATCGGCAGGGTTACCTTCACAAATCAGGGCGATATTCACATCGCGCtt < 1:1849442/70‑1 (MQ=255) ccAGCACCAACTGGCCCAGTAGATCGGCAGGGTTACCTTCACAAATCAGGGCGATATTCACATCGCGCtt < 1:1620254/70‑1 (MQ=255) cAGCACCCACTGGCCCAGTAGATCGGCAGGGTTACCTTCACAAATCAGGGCg < 1:164295/52‑1 (MQ=255) cAGCACCCACTGGCCCAGTAGATCGGCAGGGTTACCTTCACAAATCAGGGCGATATTCACATCGCGCTTGa < 1:3556885/71‑1 (MQ=255) aGCACCCACTGGCCCAGTAGATCGGCAGGGTTACCTTCACAAATCAGGGCGATATTca > 1:142697/1‑58 (MQ=255) gCACCCACTGGCCCAGTAGATCGGCAGGGTTACCTTCACAAATCAGGGCGATATTCACATCGCGCTTGATa > 1:3042103/1‑71 (MQ=255) gCACCCACTGGCCCAGTAGATCGGCAGGGTTACCTTCACAAATCAGGGCGATATTCACATCGCGCTTGATa > 1:4098924/1‑71 (MQ=255) gCACCCACTGGCCCAGTAGATCGGCAGGGTTACCTTCACAAATCAGGGCGATATTCACATCGCGCTTGATa > 1:2921762/1‑71 (MQ=255) cACCCACTGGCCCAGTAGATCGGCAGGGTTACCTTCACAAATCAGGGCGATAtt < 1:2906213/54‑1 (MQ=255) cACCCACTGGCCCAGTAGATCGGCAGGGTTACCTTCACAAATCAGGGCGATAtt < 1:3430790/54‑1 (MQ=255) cACCCACTGGCCCAGTAGATCGGCAGGGTTACCTTCACAAATCAGGGCGATATTCACATCGCGCTTGATAc < 1:1759037/71‑1 (MQ=255) aCCCACTGGCCCAGTAGATCGGCAGGGTTACCTTCACAAATCAGGGCGATATTCACATCGCGCTTGATAcc < 1:2030045/71‑1 (MQ=255) aCCCACTGGCCCAGTAGATCGGCAGGGTTACCTTCACAAATCAGGGCGATATTCACATCGCGCTTGATAcc < 1:2462637/71‑1 (MQ=255) cccACTGGCCCAGTAGATCGGCAGGGTTACCTTCACAAATCAGGGCGATATTCACATCGCGCTTGATACca < 1:2687333/71‑1 (MQ=255) aCTGGCCCAGTAGATCGGCAGGGTTACCTTCACaa < 1:777451/35‑1 (MQ=255) aCTGGCCCAGTAGATCGGCAGGGTTACCTTCACAAATCAGGGc < 1:876950/43‑1 (MQ=255) aCTGGCCCAGTAGATCGGCAGGGTTACCTTCACAAATCAGGGCGATATTCACATCGCGCt < 1:3111060/60‑1 (MQ=255) aCTGGCCCAGTAGATCGGCAGGGTTACCTTCACAAATCAGGGCGATATTCACATCGCGCTTGa > 1:1550663/1‑63 (MQ=255) aCTGGCCCAGTAGATCGGCAGGGTTACCTTCACAAATCAGGGCGATATTCACATCGCGCTTGa < 1:1305178/63‑1 (MQ=255) aCTGGCCCAGTAGATCGGCAGGGTTACCTTCACAAATCAGGGCGATATTCACATCGCGCTTGAt < 1:3588268/64‑1 (MQ=255) aCTGGCCCAGTAGATCGGCAGGGTTACCTTCACAAATCAGGGCGATATTCACATCGCGCTTGATACcaca > 1:4166119/1‑70 (MQ=255) cTGGCCCAGTAGATCGGCAGGGTTACCTTCACAAATCAGGGCGATATTCACATCGCGCTTGATACCACATa < 1:3313256/71‑1 (MQ=255) cTGGCCCAGTAGATCGGCAGGGGTACCTTCACAAATCAGGGCGATATTCACATCGCGCTTGATACCACATa < 1:3530362/71‑1 (MQ=255) tGGCCCAGTAGATCGGCAGGGTTACCTTCACAAATCAGGGCGa < 1:2694340/43‑1 (MQ=255) tGGCCCAGCAGATCGGCAGGGTTACCTTCACAAATCAGGGCGATATTCACGTCGCGCTTGATa > 1:2103495/1‑63 (MQ=255) tGGCCCAGCAGATCGGCAGGGTTACCTTCACAAATCAGGGCGATATTCACGTCGCGCTTGATa > 1:1301571/1‑63 (MQ=255) tGGCCCAGCAGATCGGCAGGGTTACCTTCACAAATCAGGGCGATATTCACGTCGCGCTTGATa > 1:3645771/1‑63 (MQ=255) ggCCCAGTAGATCGGCAGGGTTACCTTCACAAATCAGGGCGATATTCACATCGCGCTTGATa > 1:768372/1‑62 (MQ=255) ggCCCAGTAGATCGGCAGGGTTACCTTCACAAATCAGGGCGATATTCACATCGCGCTTGATACCACATACt > 1:2606488/1‑71 (MQ=255) gCCCAGTAGATCGGCAGGGTTACCTTCACAAATCAGGGCGATATTCACATCGCGCTTGATACCACATAc < 1:3773239/69‑1 (MQ=255) cccAGTAGATCGGCAGGGTTACCTTCACAAATCAGGGCGATATTCACATCGCGCTTGATACCACATACTTc < 1:2225348/71‑1 (MQ=255) cccAGTAGATCGGCAGGGTTACCTTCACAAATCAGGGCGATATTCACATCGCGCTTGATACCACATACTTc < 1:2792404/71‑1 (MQ=255) ccAGTAGATCGGCAGGGTTACCTTCACAAATCAGGGCGATATTCACATCGCGCTTGATACCACATACTTCa < 1:1594181/71‑1 (MQ=255) ccAGTAGATCGGCAGGGTTACCTTCACAAATCAGGGCGATATTCACATCGCGCTTGATACCACATACTTCa < 1:149769/71‑1 (MQ=255) ccAGTAGATCGGCAGGGTTACCTTCACAAATCAGGGCGATATTCACATCGCGCTTGATACCACATACTTCa < 1:1063022/71‑1 (MQ=255) aGTAGATCGGCAGGGTTACCTTCACAAATCAGGGCGATATTCACATCGCGCTTGATACCACATACTTCAAc > 1:287050/1‑71 (MQ=255) gTAGATCGGCAGGGTTACCTTCACAAATCAGGGCGa < 1:3805634/36‑1 (MQ=255) gTAGATCGGCAGGGTTACCTTCACAAATCAGGGCGATATTc < 1:2194774/41‑1 (MQ=255) gTAGATCGGCAGGGTTACCTTCACAAATCAGGGCGATATTCACATCGCGCTTGATACcaca > 1:71840/1‑61 (MQ=255) gTAGATCGGCAGGGTTACCTTCACAAATCAGGGCGATATTCACATCGCGCTTGATACCACATACTTCAAcc < 1:1693792/71‑1 (MQ=255) tAGATCGGCAGGGTTACCTTCACAAATCAGGGCGa > 1:3630606/1‑35 (MQ=255) | GCTTTGGCTTCATCTTCGTCGATGATGCTCATGGCAAATCCGACGTGTACCAGCACCCACTGGCCCAGTAGATCGGCAGGGTTACCTTCACAAATCAGGGCGATATTCACATCGCGCTTGATACCACATACTTCAACC > W3110S.gb/3138421‑3138558 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |