| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 3,138,531 | A→G | 7.6% | D30D (GAT→GAC) | hybG ← | hydrogenase 2 accessory protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,138,531 | 0 | A | G | 7.6% | 204.8 / 7.6 | 79 | D30D (GAT→GAC) | hybG | hydrogenase 2 accessory protein |
| Reads supporting (aligned to +/- strand): ref base A (37/36); new base G (4/2); total (41/38) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.76e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.45e-01 | |||||||||||
ACGTGTACCAGCACCCACTGGCCCAGTAGATCGGCAGGGTTACCTTCACAAATCAGGGCGATATTCACATCGCGCTTGATACCACATACTTCAACCTGCGCAAGCTGGTGAATATCTTCACCGACAGCCAGCACCTGGC > W3110S.gb/3138463‑3138601 | aCGTGTACCAGCACCCACTGGCCCAGTAGATCGGCAGGGTTACCTTCACAAATCAGGGCGATATTCACATc > 1:1027541/1‑71 (MQ=255) cGTGTACCAGCACCCACTGGCCCAGTAGATCGGCAGGGTTACCTTCACAAATCAGGGCGATATTCACATcg < 1:1847214/71‑1 (MQ=255) gtgtACCAGCACCCACTGGCCCAGTAGATCGGCAGGGTTACCTTCACAAATCAGGGCGATATTCACATcgc < 1:810628/71‑1 (MQ=255) aCCAGCACCCACTGGCCCAGTAGATCGGCAGGGTTACCTTCACAAATCAGGGCGATATTCACATCGCGCtt > 1:2979917/1‑71 (MQ=255) ccAGCACCCACTGGCCCAGTAGATCGGCAGGGTTACCTTCACAAATCAGGGCGATATTCACATCGCGCtt < 1:947229/70‑1 (MQ=255) ccAGCACCCACTGGCCCAGTAGATCGGCAGGGTTACCTTCACAAATCAGGGCGATATTCACATCGCGCtt < 1:1849442/70‑1 (MQ=255) ccAGCACCCACTGGCCCAGTAGATCGGCAGGGTTACCTTCACAAATCAGGGCGATATTCACATCGCGCtt < 1:3760229/70‑1 (MQ=255) ccAGCACCAACTGGCCCAGTAGATCGGCAGGGTTACCTTCACAAATCAGGGCGATATTCACATCGCGCtt < 1:1620254/70‑1 (MQ=255) cAGCACCCACTGGCCCAGTAGATCGGCAGGGTTACCTTCACAAATCAGGGCGATATTCACATCGCGCTTGa < 1:3556885/71‑1 (MQ=255) gCACCCACTGGCCCAGTAGATCGGCAGGGTTACCTTCACAAATCAGGGCGATATTCACATCGCGCTTGATa > 1:2921762/1‑71 (MQ=255) gCACCCACTGGCCCAGTAGATCGGCAGGGTTACCTTCACAAATCAGGGCGATATTCACATCGCGCTTGATa > 1:3042103/1‑71 (MQ=255) gCACCCACTGGCCCAGTAGATCGGCAGGGTTACCTTCACAAATCAGGGCGATATTCACATCGCGCTTGATa > 1:4098924/1‑71 (MQ=255) cACCCACTGGCCCAGTAGATCGGCAGGGTTACCTTCACAAATCAGGGCGATATTCACATCGCGCTTGATAc < 1:1759037/71‑1 (MQ=255) aCCCACTGGCCCAGTAGATCGGCAGGGTTACCTTCACAAATCAGGGCGATATTCACATCGCGCTTGATAcc < 1:2462637/71‑1 (MQ=255) aCCCACTGGCCCAGTAGATCGGCAGGGTTACCTTCACAAATCAGGGCGATATTCACATCGCGCTTGATAcc < 1:2030045/71‑1 (MQ=255) cccACTGGCCCAGTAGATCGGCAGGGTTACCTTCACAAATCAGGGCGATATTCACATCGCGCTTGATACca < 1:2687333/71‑1 (MQ=255) aCTGGCCCAGTAGATCGGCAGGGTTACCTTCACAAATCAGGGCGATATTCACATCGCGCt < 1:3111060/60‑1 (MQ=255) aCTGGCCCAGTAGATCGGCAGGGTTACCTTCACAAATCAGGGCGATATTCACATCGCGCTTGa > 1:1550663/1‑63 (MQ=255) aCTGGCCCAGTAGATCGGCAGGGTTACCTTCACAAATCAGGGCGATATTCACATCGCGCTTGa < 1:1305178/63‑1 (MQ=255) aCTGGCCCAGTAGATCGGCAGGGTTACCTTCACAAATCAGGGCGATATTCACATCGCGCTTGAt < 1:3588268/64‑1 (MQ=255) aCTGGCCCAGTAGATCGGCAGGGTTACCTTCACAAATCAGGGCGATATTCACATCGCGCTTGATACcaca > 1:4166119/1‑70 (MQ=255) cTGGCCCAGTAGATCGGCAGGGTTACCTTCACAAATCAGGGCGATATTCACATCGCGCTTGATACCACATa < 1:3313256/71‑1 (MQ=255) cTGGCCCAGTAGATCGGCAGGGGTACCTTCACAAATCAGGGCGATATTCACATCGCGCTTGATACCACATa < 1:3530362/71‑1 (MQ=255) tGGCCCAGCAGATCGGCAGGGTTACCTTCACAAATCAGGGCGATATTCACGTCGCGCTTGATa > 1:2103495/1‑63 (MQ=255) tGGCCCAGCAGATCGGCAGGGTTACCTTCACAAATCAGGGCGATATTCACGTCGCGCTTGATa > 1:1301571/1‑63 (MQ=255) tGGCCCAGCAGATCGGCAGGGTTACCTTCACAAATCAGGGCGATATTCACGTCGCGCTTGATa > 1:3645771/1‑63 (MQ=255) ggCCCAGTAGATCGGCAGGGTTACCTTCACAAATCAGGGCGATATTCACATCGCGCTTGATa > 1:768372/1‑62 (MQ=255) ggCCCAGTAGATCGGCAGGGTTACCTTCACAAATCAGGGCGATATTCACATCGCGCTTGATACCACATACt > 1:2606488/1‑71 (MQ=255) gCCCAGTAGATCGGCAGGGTTACCTTCACAAATCAGGGCGATATTCACATCGCGCTTGATACCACATAc < 1:3773239/69‑1 (MQ=255) cccAGTAGATCGGCAGGGTTACCTTCACAAATCAGGGCGATATTCACATCGCGCTTGATACCACATACTTc < 1:2792404/71‑1 (MQ=255) cccAGTAGATCGGCAGGGTTACCTTCACAAATCAGGGCGATATTCACATCGCGCTTGATACCACATACTTc < 1:2225348/71‑1 (MQ=255) ccAGTAGATCGGCAGGGTTACCTTCACAAATCAGGGCGATATTCACATCGCGCTTGATACCACATACTTCa < 1:1063022/71‑1 (MQ=255) ccAGTAGATCGGCAGGGTTACCTTCACAAATCAGGGCGATATTCACATCGCGCTTGATACCACATACTTCa < 1:1594181/71‑1 (MQ=255) ccAGTAGATCGGCAGGGTTACCTTCACAAATCAGGGCGATATTCACATCGCGCTTGATACCACATACTTCa < 1:149769/71‑1 (MQ=255) aGTAGATCGGCAGGGTTACCTTCACAAATCAGGGCGATATTCACATCGCGCTTGATACCACATACTTCAAc > 1:287050/1‑71 (MQ=255) gTAGATCGGCAGGGTTACCTTCACAAATCAGGGCGATATTCACATCGCGCTTGATACcaca > 1:71840/1‑61 (MQ=255) gTAGATCGGCAGGGTTACCTTCACAAATCAGGGCGATATTCACATCGCGCTTGATACCACATACTTCAAcc < 1:1693792/71‑1 (MQ=255) aGATCGGCAGGGTTACCTTCACAAATCAGGGCGATATTCACATCGCGCTTGa > 1:3889436/1‑52 (MQ=255) aGATCGGCAGGGTTACCTTCACAAATCAGGGCGATATTCACATCGCGCTTGATACCACATACTTCAACCTg > 1:3436210/1‑71 (MQ=255) gATCGGCAGGGTTACCTTCACAAATCAGGGCGATATTCACATCGCGCTTGATACCACATACTTCAACCTgc > 1:1589200/1‑71 (MQ=255) gATCGGCAGGGTTACCTTCACAAATCAGGGCGATATTCACATCGCGCTTGATACCACATACTTCAACCTgc > 1:3006558/1‑71 (MQ=255) tCGGCAGGGTTACCTTCACAAATCAGGGCGATATTCACGTCGCGCTTGATAc < 1:4358470/52‑1 (MQ=255) tCGGCAGGGTTACCTTCACAAATCAGGGCGATATTCACGTCGCGCTTGATAc < 1:1984610/52‑1 (MQ=255) cGGCAGGGTTACCTTCACAAATCAGGGCGATATTCACATCGCGCtt > 1:1303616/1‑46 (MQ=255) cGGCAGGGTTACCTTCACAAATCAGGGCGATATTCACATCGCGCTTGATACCACATACTTCAACCTgcgc > 1:3795777/1‑70 (MQ=255) cGGCAGGGTTACCTTCACAAATCAGGGCGATATTCACATCGCGCTTGATACCACATACTTCAACCTgcgc > 1:4298517/1‑70 (MQ=255) gCAGGGTTACCTTCACAAATCAGGGCGATATTCACATCGAGCTTGATACAACATACTTCAACCTGCGCAAg > 1:1123189/1‑71 (MQ=255) aGGGTTACCTTCACAAATCAGGGCGATATTCACATCGCGCTTGATACCACATACTTCAACCTGCGCAAGCt > 1:2951182/1‑71 (MQ=255) ggTTACCTTCACAAATCAGGGCGATATTCACATCGCGCTTGATACCACATACTTCAACCTGCGCAAGCTgg > 1:74415/1‑71 (MQ=255) ttACCTTCACAAATCAGGGCGATATTCACATCGCGCTTGATa > 1:911763/1‑42 (MQ=255) ttACCTTCACAAATCAGGGCGATATTCACATCGCGCTTGATACCACATACTTCAACCTGCGCAAGCTGGt > 1:1419533/1‑70 (MQ=255) ttACCTTCACAAATCAGGGCGATATTCACATCGCGCTTGATACCACATACTTCAACCTGCGCAAGCTGGTg < 1:1435218/71‑1 (MQ=255) ttACCTTCACAAATCAGGGCGATATTCACATCGCGCTTGATACCACATACTTCAACCTGCGCAAGCTGGTg < 1:3196394/71‑1 (MQ=255) ttACCTTCACAAATCAGGGCGATATTCACATCGCGCTTGATACCACATACTTCAACCTGCGCAAGCTGGTg < 1:155523/71‑1 (MQ=255) tACCTTCACAAATCAGGGCGATATTCACATCGCGCTTGATACCACAt > 1:3090696/1‑47 (MQ=255) tACCTTCACAAATCAGGGCGATATTCACATCGCGCTTGATACCACATACTTCAACCTGCGCaa > 1:2988212/1‑63 (MQ=255) aCCTTCACAAATCAGGGCGATATTCACATCGCGCTTGATACCACATACTTCAACCTGCGCAAGCTGGTGaa > 1:3905552/1‑71 (MQ=255) aCCTTCACAAATCAGGGCGATATTCACATCGCGCTTGATACCACATACTTCAACCTGCGCAAGCTGGTGaa > 1:3200541/1‑71 (MQ=255) aCCTTCACAAATCAGGGCGATATTCACATCGCGCTTGATACCACATACTTCAACCTGCGCAAGCTGGTGaa > 1:2682641/1‑71 (MQ=255) ccTTCACAAATCAGGGCGATATTCACATCGCGCTTGATACCACATACTTCAACCTGCGCAAGCTGGTGAat < 1:2336721/71‑1 (MQ=255) cTTCACAAATCAGGGCGATATTCACATCGCGCTTGATACCACATACTTCa < 1:457608/50‑1 (MQ=255) cTTCACAAATCAGGGCGATATTCACATCGCGCTTGATACCACATACTTCa < 1:215396/50‑1 (MQ=255) tCACAAATCAGGGCGATATTCACATCGCGCTTGATAcc < 1:550682/38‑1 (MQ=255) tCACAAATCAGGGCGATATTCACATCGCGCTTGATACCACATACTTCAACCTGCGCAAGCTGGTGAATATc > 1:3622529/1‑71 (MQ=255) tCACAAATCAGGGCGATATTCACATCGCGCTTGATACCACATACTTCAACCTGCGCAAGCTGGTGAATATc > 1:753498/1‑71 (MQ=255) cacaAATCAGGGCGATATTCACATCGCGCTTGATACCACATa > 1:1644644/1‑42 (MQ=255) cacaAATCAGGGCGATATTCACATCGCGCTTGATACCACATACTTCAACCTGCGCAAGCTGGTGAATATCt > 1:320352/1‑71 (MQ=255) cacaAATCAGGGCGATATTCACATAGCGCTTGATACCACATACTTCAACCTGCGCAAGCTGGTGAATATCt > 1:363319/1‑71 (MQ=255) cacaAATCAGGGCGATATTAACATCGCGCTTGATACCACATa > 1:2137781/1‑42 (MQ=255) caAATCAGGGCGATATTCACGTCGCGCTTGATACCACATACTTCAACCTGCGCAAGCTGGTGAATATc > 1:4072999/1‑68 (MQ=255) caAATCAGGGCGATATTCACATCGCGCTTGATACCACATACTTCAACCTGCGCAAGCTGGTGAATATCTTc < 1:716120/71‑1 (MQ=255) aaaTCAGGGCGATATTCACATCGCGCTTGATACCACATACTTc < 1:3069476/43‑1 (MQ=255) aaaTCAGGGCGATATTCACATCGCGCTTGATACCACATACTTCAACCTGCGCAAGCTGGTGAATATCTTCa < 1:2132263/71‑1 (MQ=255) aTCAGGGCGATATTCACATCGCGCTTGATACCACATACTTCAACCTGCGCAAGCTGGTGAATATCTTCAcc < 1:2105862/71‑1 (MQ=255) cAGGGCGATATTCACATCGCGCTTGATACCACATACTTCAAcc > 1:3509651/1‑43 (MQ=255) aGGGCGATATTCACATCGCGCTTGATACCACATACTTCAACCTGCGCAAGCTGGTGAATATCTTCACCGa > 1:152652/1‑70 (MQ=255) gCGATATTCACATCGCGCTTGATACCACATACTTCAACCTGCGCAAGCTGGTg < 1:3317039/53‑1 (MQ=255) ttCACATCGCGCTTGATACCACATACTTCAACCTGCGCAAGCTGGTGAATATCTTCACCGAc < 1:57465/62‑1 (MQ=255) tCACATCGCGCTTGATACCACATACTTCAACCTGCGCAAGCTGGTGAatat > 1:2168989/1‑51 (MQ=255) cacaTCGCGCTTGATACCACATACTTCAACCTGCGCAAGCTGGTGAATATCTTCACCGACAGCCAGCAcct > 1:2377763/1‑71 (MQ=255) cacaTCGCGCTTGATACCACATACTTCAACCTGCGCAAGCTGGTGAATATCTTCACCGACAGCCAGCAcct > 1:3820169/1‑71 (MQ=255) cacaTCGCGCTTGATACCACATACTTCAACCTGCGCAAGCTGGTGAATATCTTCACCGACAGCCAGCAcct > 1:3717595/1‑71 (MQ=255) cacaTCGCGCTTGATACCACATACTTCAACCTGCGCAAGCTGGTGAATATCTTCACCGACAGCCAGCAcc < 1:1637892/70‑1 (MQ=255) acaTCGCGCTTGATACCACATACTTCAACCTGCGCAAGCTGGTGAATATCTTCACCGACAGCCAGCAcctg < 1:690219/71‑1 (MQ=255) acaTCGCGCTTGATACCACATACTTCAACCTGCGCAAGCTGGTGAATATCTTCACCGACAGCCAGCAcctg < 1:2478150/71‑1 (MQ=255) aTCGCGCTTGATACCACATACTTCAACCTTCGCAAGCTGGTGAATATCTTCACCGACAGCCAGCAcctggc < 1:3641506/71‑1 (MQ=255) aTCGCGCTTGATACCACATACTTCAACCTGCGCAAGCTGGTGAATATCTTc < 1:1198070/51‑1 (MQ=255) | ACGTGTACCAGCACCCACTGGCCCAGTAGATCGGCAGGGTTACCTTCACAAATCAGGGCGATATTCACATCGCGCTTGATACCACATACTTCAACCTGCGCAAGCTGGTGAATATCTTCACCGACAGCCAGCACCTGGC > W3110S.gb/3138463‑3138601 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |