| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 3,415,880 | A→T | 7.0% | P331P (CCA→CCT) | acrF → | multidrug efflux system protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,415,880 | 0 | A | T | 7.0% | 228.0 / 8.8 | 85 | P331P (CCA→CCT) | acrF | multidrug efflux system protein |
| Reads supporting (aligned to +/- strand): ref base A (47/32); new base T (2/4); total (49/36) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.94e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.65e-01 | |||||||||||
AAACTGGCGGAATTACAGCCATTCTTCCCGCAGGGAATGAAGGTTCTCTACCCTTATGACACCACGCCATTCGTCCAGCTTTCTATTCACGAAGTGGTAAAAACGCTGTTCGAAGCCATTATGCTGGTGTTCCTGGTG > W3110S.gb/3415812‑3415949 | aaaCTGGCGGAATTACAGCCATTCTTCCCGCAGGGAATGAAGGTTCTCTACCCTTATGACACCACGCCAtt > 1:3328075/1‑71 (MQ=255) aaCTGGCGGAATTACAGCCATTCTTCCCGCAGGGAATGAAGGTTCTCTACCCTTATGACACCACGCCATTc > 1:1558527/1‑71 (MQ=255) aCTGGCGGAATTACAGCCATTCTTCCCGCAGGGAATTAAGGTTCTCTACCCTTATGACACCACGCCATTCg > 1:32242/1‑71 (MQ=255) aCTGGCGGAATTACAGCCATTCTTCCCGCAGGGAATGAAGGTTCTCTACCCTTATGACACCACGCCATTCg > 1:4002204/1‑71 (MQ=255) aCTGGCGGAATTACAGCCATTCTTCCCGCAGGGAATGAAGGTTCTCTACCCTTATGACACCACGCCATTCg > 1:3450605/1‑71 (MQ=255) cTGGCGGAATTACAGCCATTCTTCCCGCAGGGAATGAAGGTTCTTTACCCTTATGACACCACGCCTTTCGt < 1:1291274/71‑1 (MQ=255) cTGGCGGAATTACAGCCATTCTTCCCGCAGGGAATGAAGGTTCTTTACCCTTATGACACCACGCCTTTCGt < 1:3363154/71‑1 (MQ=255) cTGGCGGAATTACAGCCATTCTTCCCGCAGGGAATGAAGGTTCTTTACCCTTATGACACCACGCCTTTCGt < 1:3184732/71‑1 (MQ=255) cGGAATTACAGCCATTCTTCCCGCAGGGAATGAAGGTTCTCTACCCTTATGACACCACGCCATTCGTCCAg < 1:3818479/71‑1 (MQ=255) ggAATTACAGCCATTCTTCCCGCAGGGAATGAAGGTTCTTTACCCTTATGACACCACGCCTTTCGt < 1:252198/66‑1 (MQ=255) gAATTACAGCCATTCTTCCCGCAGGGAATGAAGGTTCTCTACCCTTATGACACCACGCCATTCGTCCAGCt > 1:1031002/1‑71 (MQ=255) gAATTACAGCCATTCTTCCCGCAGGGAATGAAGGTTCTCTACCCTTATGACACCACGCCATTCGTCCAGCt < 1:3922977/71‑1 (MQ=255) gAATTACACCCATTCTTCCCGCAGGGAATGAAGGTTCTCTACCCTTATGACACCACGCCATTCGTCCAGCt > 1:222664/1‑71 (MQ=255) aTTACAGCCATTCTTCCCGCAGGGAATGAAGGTTCTCTACCCTTATGACAGCACGCCATTCGTCCAGCttt > 1:3565234/1‑71 (MQ=255) aTTACAGCCATTCTTCCCGCAGGGAATGAAGGTTCTCTACCCTTATGACACCACGCCATTCGTCCAGCttt > 1:27047/1‑71 (MQ=255) aTTACAGCCATTCTTCCCGCAGGGAATGAAGGTTCTCTACCCTTATGACACCACGCCATTCGTCCAGCttt > 1:46904/1‑71 (MQ=255) ttACAGCCATTCTTCCCGCAGGGAATGAAGGTTCTCTACCCTTATGACACCACGCCATTCGTCCAGCTTTc > 1:3537065/1‑71 (MQ=255) ttACAGCCATTCTTCCCGCAGGGAATGAAGGTTCTCTACCCTTATGACACCACGCCATTCGTCCAGCTTTc > 1:221881/1‑71 (MQ=255) tACAGCCATTCTTCCCGCAGGGAATGAAGGTTCTCTACCCTTATGACACCACGCCATTCGTCCAGCTTTc > 1:3896638/1‑70 (MQ=255) aCAGCCATTCTTCCCGCAGGGAATGAAGGTTCTCTACCCTTATGACACCACGCCATTCGTCCAGCTTTCTa > 1:3376114/1‑71 (MQ=255) cAGCCATTCTTCCCGCAGGGAATGAAGGTTCTCTACCCTTATGACACCACGCCATTCGTCCAGCTTTCTa < 1:2523770/70‑1 (MQ=255) aGCCATTCTTCCCGCAGGGAATGAAGGTTCTCTACCCTTATGACACCACGCCATTCGTCCAGCTTTCTAtt > 1:2737829/1‑71 (MQ=255) aTTCTTCCCGCAGGGAATGAAGGTTCTTTACCCTTATGACACCACGCCTTTCGTCCAGc > 1:4066446/1‑59 (MQ=255) cttcCCGCAGGGAATGAAGGTTCTCTACCCTTATGACACCACGCCATTCGTCCAGc > 1:2246471/1‑56 (MQ=255) cttcCCGCAGGGAATGAAGGTTCTCTACCCTTATGACACCACGCCATTCGTCCAGCTTTCTATTCACGAAg > 1:79575/1‑71 (MQ=255) cttcCCGCAGGGAATGAAGGTTCTCTACCCTTATGACACCACGCCATTCGTCCAGCTTTCTATTCACGAAg > 1:2936774/1‑71 (MQ=255) cttcCCGCAGGGAATGAAGGTTCTCTACCCTTATGACACCACGCCATTCGTCCAGCTTTCTATTCACGAAg > 1:1707132/1‑71 (MQ=255) ttcCCGGAGGGAATGAAGGTTCTCTACCCTTATGACACCACGCCATTCGTCCAGCTTTCTATTCACg > 1:1370101/1‑67 (MQ=255) cccGCAGGGAATGAAGGTTCTCTACCCTTATGACACCACGCCATTCGTCCAGCTTTCTATTCACGAAGt < 1:33680/69‑1 (MQ=255) cccGCAGGGAATGAAGGTTCTCTACCCTTATGACACCACGCCATTCGTCCAGCTTTCTATTCACGAAGTg < 1:1495425/70‑1 (MQ=255) cGCAGGGAATGAAGGTTCTCTACCCTTATGACACCACGCCATTCGTCCAGCTTTCTATTCACGAAGTGGt > 1:3541505/1‑70 (MQ=255) cAGGGAATGAAGGTTCTCTACCCTTATGACACCACGCCATTCGTCCAGCTTTCTATTCACGAAGTGGTaaa < 1:3402330/71‑1 (MQ=255) aGGGAATGAAGGTTCTCTACCCTTATGACACCACGCCATTCGTCCAGCTTTCTATTCACGAAGTGGTaaa < 1:1238784/70‑1 (MQ=255) aaTGAAGGTTCTCTACCCTTATGACACCACGCCATTCGTCCAGCTTTCTATTCACGAAGTGGTaaaaa > 1:2132862/1‑68 (MQ=255) aaTGAAGGTTCTCTACCCTTATGACACCACGCCATTCGTCCAGCTTTCTATTCACGAAGTGGTaaaaa > 1:3515428/1‑68 (MQ=255) tGAAGGTTCTCTACCCTTATGACACCACGCCATTCGTCCAGCTTTc < 1:2139963/46‑1 (MQ=255) tGAAGGTTCTCTACCCTTATGACACCACGCCATTCGTCCAGCTTTc < 1:3368633/46‑1 (MQ=255) tGAAGGTTCTCTACCCTTATGACACCACGCCATTCGTCCAGCTTTCTATTCACGAAGt < 1:190565/58‑1 (MQ=255) aaGGTTCTCTACCCTTATGACACCACGCCATTCGTCCAGCttt > 1:3910781/1‑43 (MQ=255) aaGGTTCTCTACCCTTATGACACCACGCCATTCGTCCAGCTTTCTATTCACGAAGt > 1:4280633/1‑56 (MQ=255) aaGGTTCTCTACCCTTATGACACCACGCCATTCGTCCAGCTTTCTATTCACGAAGt > 1:4218249/1‑56 (MQ=255) aaGGTTCTCTACCCTTATGACACCACGCCATTCGTCCAGCTTTCTATTCACGAAGTGGTAAAAACGCTGtt > 1:4284497/1‑71 (MQ=255) aaGGTTCTCTACCCTTATGACACCACGCCATACGTCCAGCTTTCTATTCACGAAGTGGTAAAAACGCTGtt > 1:146667/1‑71 (MQ=255) ggTTCTCTACCCTTATGACACCACGCCATTCGTCCAGCTTTCTATTCACGAAGTGGTAAAAACGCTGTTCg < 1:1941964/71‑1 (MQ=255) ggTTCTCTACCCTTATGACACCACGCCATTCGTCCAGCTTTCTATTCACGAAGTGGTAAAAACGCTGTTCg < 1:495293/71‑1 (MQ=255) ggTTCTCTACCCTTATGACACCACGCCATTCGTCCAGCTTTCTATTCACGAAGTGGTAAAAACGCTGTTCg < 1:256324/71‑1 (MQ=255) gTTCTCTACCCTTATGACACCACGCCATTCGTCCAGCTTTCTATTCACg < 1:3552915/49‑1 (MQ=255) gTTCTCTACCCTTATGACACCACGCCATTCGTCCAGCTTTCTATTCACg < 1:3397562/49‑1 (MQ=255) tctcTACCCTTATGACACCACGCCATTCGTCCAGCTTTCTATTCACGAAGTg > 1:189749/1‑52 (MQ=255) ctttACCCTTATGACACCACGCCTTTCGTCCAGCTTTCTATCCAGGAAGTGGTGAAAACGCTGTTCGAAGc > 1:1005542/4‑71 (MQ=21) ctctACCCTTATGACACCACGCCATTCGTCCAGCTTTCTATTCACGAAGt < 1:3360699/50‑1 (MQ=255) tctACCCTTATGACACCACGCCATTCGTCCAGCTTTCTa > 1:2486125/1‑39 (MQ=255) tctACCCTTATGACACCACGCCATTCGTCCAGCTTTCTa > 1:3647204/1‑39 (MQ=255) tctACCCTTATGACA‑CACGCCATTCGTCCAGCTTTCTa > 1:429580/1‑38 (MQ=38) ctACCCTTATGACACCACGCCATTCGTCCAGCTTTCTATTCACGAAGTGGTAAAAACGCTGtt > 1:1352145/1‑63 (MQ=255) ctACCCTTATGACACCACGCCATTCGTCCAGCTTTCTATTCACGAAGTGGTAAAAACGCTGtt > 1:2436003/1‑63 (MQ=255) ctACCCTTATGACACCACGCCATTCGTCCAGCTTTCTATTCACGAAGTGGTAAAAACGCTGTTCGAAGCCa > 1:2669585/1‑71 (MQ=255) tACCCTTATGACACCACGCCATTCGTCCAGCTTTc < 1:923944/35‑1 (MQ=255) cccTTATGACACCACGCCATTCGTCCAGCTTTCTATTCACGAAGTGGTAAAAACGCTGTTCGAAGCCa < 1:3951127/68‑1 (MQ=255) cccTTATGACACCACGCCATTCGTCCAGCTTTCTATTCACGAAGTGGTAAAAACGCTGTTCGAAGCCATTa > 1:3803666/1‑71 (MQ=255) ccTTATGACACCACGCCATTCGTCCAGCTTTCTATTCACGAAGTGGTAAAAACGCTGTTCGAAGCCAt < 1:1997156/68‑1 (MQ=255) cTTATGACACCACGCCATTCGTCCAGCTTTCTATTCACGAAGTGGTAAAAACGCTg < 1:4161939/56‑1 (MQ=255) ttATGACACCACGCCATTCGTCCAGCTTTCTATTCACGa < 1:4343462/39‑1 (MQ=255) ttATGACACCACGCCATTCGTCCAGCTTTCTATTCACGAAGTGGTAAAAACGCTg > 1:2684529/1‑55 (MQ=255) tATGACACCACGCCATTCGTCCAGCTTTCTATTCACGAAGTGGTAAAAACGCTGTTCGAAGCCATTa > 1:4243694/1‑67 (MQ=255) tATGACACCACGCCATTCGTCCAGCTTTCTATTCACGAAGTGGTAAAAACGCTGTTCGAAGCCATTATGCt > 1:3258488/1‑71 (MQ=255) tATGACACCACGCCATTCGTCCAGCTTTCTATTCACGAAGTGGTAAAAACGCTGTTCGAAGCCACTa > 1:3012146/1‑67 (MQ=255) tATGACACAACGCCATTCGTCCAGCTTTCTATTCACGAAGTGGTAAAAACGCTGTTCGAAGCCATTa > 1:281696/1‑67 (MQ=255) aTGACACCACGCCATTCGTCCAGCTTTCTATTCACGAAGTGGTAAAAACGCTGTTCGAAGCCATTa > 1:3921516/1‑66 (MQ=255) aTGACACCACGCCATTCGTCCAGCTTTCTATTCACGAAGTGGTAAAAACGCTGTTCGAAGCCATTATGc < 1:2322665/69‑1 (MQ=255) aTGACACCACGCCATTCGTCCAGCTTTCTATTCACGAAGTGGTAAAAACGCTGTTCGAAGCCATTATGc < 1:2297027/69‑1 (MQ=255) aTGACACCACGCCATTCGTCCAGCTTTCTATTCACGAAGTGGTAAAAACGCTGTTCGAAGCCATTATGc < 1:2133089/69‑1 (MQ=255) tGACACCACGCCATTCGTCCAGCTTTCTATTCACGAAGTGGTAAAAACGCTGTTCGAAGCCATTATGCTgg < 1:825074/71‑1 (MQ=255) gACACCACGCCATTCGTCCAGCTTTCTATTCACGAAGTGGTAAAAACGCTGTTCGAAGCCATTATGCTgg > 1:4318840/1‑70 (MQ=255) gACACCACGCCATTCGTCCAGCTTTCTATTCACGAAGTGGTAAAAACGCTGTTCGAAGCCATTATGCTGgt > 1:420004/1‑71 (MQ=255) acacCACGCCATTCGTCCAGCTTTCTATTCACGAAGTGGTAAAAACGCTGTTCGAAGCCATTATGCTGgtg < 1:2345050/71‑1 (MQ=255) caccacGCCATTCGTCCAGCTTTCTATTCACGAAGTGGTAAAAACGCTGTTCGAAGCCATTATGCTGgtgt < 1:1492256/71‑1 (MQ=255) cacGCCATTCGTCCAGCTTTCTATTAACGAAGTGGTAAAAACGCTGTTCGAAGCCATTATGCTGGTGTTcc > 1:3616489/1‑71 (MQ=255) cGCCATTCGTCCAGCTTTCTATTCACGAAGTGGTAAAAACGCTGTTCGAAGCCATTa > 1:1531778/1‑57 (MQ=255) cGCCATTCGTCCAGCTTTCTATTCACGAAGTGGTAAAAACGCTGTTCGAAGCCATTATGCTGGTGTTCCTg > 1:4410774/1‑71 (MQ=255) ccATTCGTCCAGCTTTCTATTCACGAAGTGGTAAAAACGCTGTTCGAAGCCATTATGCTGGTGTTCCTGGt < 1:2056033/71‑1 (MQ=255) ccATTCGTCCAGCTTTCTATTCACGAAGTGGTAAAAACGCTGTTCGAAGCCATTATGCTGGTGTTCCTGGt < 1:1774983/71‑1 (MQ=255) ccATTCGTCCAGCTTTCTATTCACGAAGTGGTAAAAACGCTGTTCGAAGCCATTATGCTGGTGTTCCTGGt < 1:3250232/71‑1 (MQ=255) cATTCGTCCAGCTTTCTATTCACGAAGTGGTAAAAACGCTGTTCGAAGCCATTATGCTGGTGTTCCTGGTg < 1:840143/71‑1 (MQ=255) cATTCGTCCAGCTTTCTATTCACGAAGTGGTAAAAACGCTGTTCGAAGCCATTATGCTCGTGTTCCTGGTg < 1:813956/71‑1 (MQ=255) aTTCGTCCAGCTTTCTATTCACGAAGTGGTAAAAACGCTGTTCGAAGCCAt < 1:4071159/51‑1 (MQ=255) | AAACTGGCGGAATTACAGCCATTCTTCCCGCAGGGAATGAAGGTTCTCTACCCTTATGACACCACGCCATTCGTCCAGCTTTCTATTCACGAAGTGGTAAAAACGCTGTTCGAAGCCATTATGCTGGTGTTCCTGGTG > W3110S.gb/3415812‑3415949 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |