New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	W3110S.gb	1547131 =	60 (1.230)	3 (0.070)	3/94	NT	4.6%	coding (298/957 nt)	yddK	hypothetical protein
	?	W3110S.gb	1547165 =	67 (1.460)					coding (264/957 nt)	yddK	hypothetical protein
Rejected: Frequency below/above cutoff threshold.

CAGTACTGGACTGCAGTAAGAAATATAATTGATGACACTAATGAAGTGACCATTGATTTATCAGGACTTAATT‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  W3110S.gb/1547203‑1547131 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑attAGTGTCATCAATTATATTTCTTACTGCAGTCCAGTACTGCATTTGTGCAAGTGGATCATTGTTGATGCC  >  W3110S.gb/1547165‑1547233                                                                                                                                                CAGTACTGGACTGCAGTAAGAAATATAATTGATGACACTAATGAAGTGACCATTGATTTATCAGGACTTAA                                                                         <  1:2697971/71‑1      CTGGACTGCAGTAAGAAATATAATTGATGACACTAATGAAGTGACCATTGATTTATCAGGACTTAATT                                                                       >  1:295362/1‑68          ACTGCAGTAAGAAATATAATTGATGACACTAATGAAGTGACCATTGATTTATCAGGACTTAATT                                                                       <  1:1626322/64‑1                                       TAATGAAGTGACCATTGATTTATCAGGACTTAATTAGTGTCATCAATTATATTTCTTACTGCAGTCCAGTA                                   >  1:1900408/1‑71                                               TGACCATTGATTTATCAGGACTTAATTAGTGTCATCAATTATATTT                                                    <  1:4351693/46‑1                                                     TTGATTTATCAGGACTTAATAAGTGTCATCAATTATATTTCTTACTGCA                                           <  1:3134153/49‑1                                                                       ATTAGTGTCATCAATTATATTTCTTACTGCAGTCCAGTACTGCATTTGTGCAAGTGGATCATTGTTGATG    <  1:4029437/70‑1                                                                       ATTAGTGTCATCAATTATATTTCTTACTGCAGTCCAGTACTGCATTTGTGCAAGTGGATCATTGTTGATG    <  1:4224643/70‑1                                                                       ATTAGTGTCATCAATTATATTTCTTACTGCAGTCCAGTACTGCATTTGTGCAAGTGGATCATTGTTGATG    <  1:621247/70‑1                                                                        TTAGTGTCATCAATTATATTTCTTACTGCAGTCCAGTACTGCATTTGTGCAAGTGGATCATTGTTGATGCC  <  1:2883279/71‑1                                                                         TAGTGTCATCAATTATATTTCTTACTGCAGTACAGTACTGCATTTGTGCAAGTGGA                >  1:1308917/1‑56                                                                                                                                                CAGTACTGGACTGCAGTAAGAAATATAATTGATGACACTAATGAAGTGACCATTGATTTATCAGGACTTAATT‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  W3110S.gb/1547203‑1547131 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑attAGTGTCATCAATTATATTTCTTACTGCAGTCCAGTACTGCATTTGTGCAAGTGGATCATTGTTGATGCC  >  W3110S.gb/1547165‑1547233

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.