| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 236,101 | A→C | 89.2% | D12A (GAT→GCT) | dnaQ → | DNA polymerase III epsilon subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 236,101 | 0 | A | C | 89.2% | 2511.0 / 138.6 | 778 | D12A (GAT→GCT) | dnaQ | DNA polymerase III epsilon subunit |
| Reads supporting (aligned to +/- strand): ref base A (44/41); new base C (378/315); total (422/356) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.46e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.50e-01 | |||||||||||
Only 100 of 782 total aligned reads displayed.
ACTGTAAAACGACAAGTCTGACATAAATGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGATACCGAAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGCCACAAGATCATTGAGATTGGTG > W3110S.gb/236032‑236169 | tgtacaactCAAGTCTGACATAAATGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGCTAcc < 1:1935666‑M2/62‑1 (MQ=255) acaactCAAGTCTGACATAAATGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGCTACcgc > 1:4047681‑M2/7‑69 (MQ=255) caactCAAGTCTGACATAAATGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGCTAccgca < 1:1031986‑M2/65‑3 (MQ=255) caactCAAGTCTGACATAAATGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGCTAccgca < 1:3608960‑M2/65‑3 (MQ=255) caactCAAGTCTGACATAAATGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGCTACCGcaa > 1:3684428‑M2/6‑71 (MQ=255) actCAAGTCTGACATAAATGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGCTACCGCAacc < 1:2467942‑M2/68‑1 (MQ=255) cAAGTCTGACATAAATGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGCTACCGCAaccacc > 1:514885/1‑71 (MQ=255) cAAGTCTGACATAAATGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGATACCGaaa > 1:3944948/1‑66 (MQ=255) aaGTCTGACATAAATGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGCTACCGCAACCACCg > 1:3422963/1‑71 (MQ=255) aaGTCTGACATAAATGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGCTACCGCAACCACCg > 1:2876267/1‑71 (MQ=255) aaGTCTGACATAAATGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGCTACCGCAACCACCg > 1:2147471/1‑71 (MQ=255) aGTCTGACATAAATGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGCTACCGCAACCACCgg < 1:4312600/71‑1 (MQ=255) aGTCTGACATAAATGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGCTACCGCAACCACCgg < 1:2530571/71‑1 (MQ=255) tCTTACATAAATGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGCTACCgca < 1:212197/61‑3 (MQ=255) tGACATAAATGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGCTACCGCAACCACCGGTAt > 1:2713080/1‑70 (MQ=255) aCATAAATGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCTCTACCGCAACCACCGGTAt < 1:970246/68‑1 (MQ=255) aCATAAATGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGCTACCGCAACCACCGGTAt < 1:2046831/68‑1 (MQ=255) aCATAAATGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGCTACCGCAACCACCGGTATg < 1:88409/69‑1 (MQ=255) aCATAAATGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGaa > 1:568677/1‑71 (MQ=255) aCATAAATGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGATACCGAAACCACCGGTAt < 1:2497957/68‑1 (MQ=255) cATAAATGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGAAc > 1:1079357/1‑71 (MQ=255) aTAAATGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGCTACCGCAaccac > 1:629439/1‑60 (MQ=255) tAAATGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGa < 1:1447752/67‑1 (MQ=255) tAAATGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGAACCa < 1:258365/71‑1 (MQ=255) aaaTGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGAAc > 1:1614789/1‑68 (MQ=255) aaaTGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGATACCGAAACCACCGGTATGAAc > 1:4097257/1‑68 (MQ=255) aaTGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGAAc > 1:3594180/1‑67 (MQ=255) aaTGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGATACCGAAACCACCGGTATGAACCAg < 1:94184/70‑1 (MQ=255) aTGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGAACCAGAt < 1:3077387/71‑1 (MQ=255) aTGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGAACCAGAt < 1:782479/71‑1 (MQ=255) tGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTAGCTACCGCAACCACCGGTATGAACCAGAtt < 1:427434/71‑1 (MQ=255) gACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGATACCGAAac > 1:3263829/1‑51 (MQ=255) aCCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTg < 1:4182890/70‑1 (MQ=255) aCCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTg < 1:4075833/70‑1 (MQ=255) aCCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGATACCGAAACCACCGGTATGAACCAGATTg < 1:1969873/70‑1 (MQ=255) cGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGCTACCGCAac > 1:714582/1‑48 (MQ=255) cGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGAACCa < 1:4467250/63‑1 (MQ=255) gCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTGGTgc < 1:2276099/71‑1 (MQ=255) cTATGAGCACTGCAATTACACGCCAGATCGTTCTCGCTACCGCAac < 1:4148199/46‑1 (MQ=255) tGAGCACTGCAATTACACGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGAAc < 1:1140328/56‑1 (MQ=255) gAGCACTGCAATTACACGCCAGATCGTTCTCGCTACCGCAACCACCg > 1:2838718/1‑47 (MQ=255) gAGCACTGCAATTACACGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCAc > 1:3758819/1‑70 (MQ=255) aGCACTGCAATTACACGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCACTa > 1:3873054/1‑71 (MQ=255) aGCACTGCAATTACACGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCACTa < 1:1317942/71‑1 (MQ=255) gCACTGCAATTACACGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGa < 1:2911674/51‑1 (MQ=255) gCACTGCAATTACACGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCACTAt < 1:1570526/71‑1 (MQ=255) cACTGCAATTACACGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCACTAt > 1:1545368/1‑70 (MQ=255) aCTGCAATTACACGCCAGATCGTTCTCGCTACCGCAACCACCgg < 1:3900076/44‑1 (MQ=255) aCTGCAATTACACGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGAAc < 1:3642284/51‑1 (MQ=255) cTGCAATTACACGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCACTa < 1:1674815/67‑1 (MQ=255) cTGCAATTACACGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCACTATGaa < 1:2996335/71‑1 (MQ=255) cTGCAATTACACGCCAGATCGTTCTCGATACCGAAACCACCGGTATGAACCAGATTGGTGCGCACTATg > 1:4405403/1‑69 (MQ=255) tGCAATTACACGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTg < 1:3290307/56‑1 (MQ=255) gCAATTACACGCCAGATCGTTCTCGCTACCGCAaccacc < 1:2326867/39‑1 (MQ=38) cAATTAGACGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGc > 1:589856/1‑71 (MQ=255) cAATTACACGCCAGATCGTTCTCTCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGc > 1:137487/1‑71 (MQ=255) cAATTACACGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAgg > 1:1503426/1‑70 (MQ=255) cAATTACACGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGc > 1:1855605/1‑71 (MQ=255) cAATTACACGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGc > 1:65953/1‑71 (MQ=255) cAATTACACGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGc > 1:1784586/1‑71 (MQ=255) cAATTACACGCCAGATCGTTCTCGATACCGAAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGc > 1:2415404/1‑71 (MQ=255) aTTACACGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGCca > 1:4214316/1‑71 (MQ=255) ttACACGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGAAc < 1:4493436/44‑1 (MQ=38) ttACACGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCACTATGa < 1:353559/64‑1 (MQ=255) ttACACGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGCcac < 1:1194505/71‑1 (MQ=255) ttACACGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGCca > 1:4542424/1‑70 (MQ=255) ttACACGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGCca > 1:2763586/1‑70 (MQ=255) ttACACGCCAGATCGTTCTCGATACCGAAACCACCGGTATGAACCa < 1:84078/46‑1 (MQ=255) tACACGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCACTAt > 1:2197803/1‑61 (MQ=255) acacGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTg > 1:3326400/1‑49 (MQ=39) cacGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGCCACAAg < 1:3463367/71‑1 (MQ=255) cacGCCAGATCGTTCTCGATACCGAAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGCCACAAg < 1:750494/71‑1 (MQ=255) acGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGCCACAAGa < 1:812841/71‑1 (MQ=255) ctccAGATCGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGCCACAAGAt < 1:3832838/69‑1 (MQ=255) cGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGCCACAAGAt < 1:2081285/71‑1 (MQ=255) cGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGCCACAAGAt < 1:3138305/71‑1 (MQ=255) cGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGCCACAAGAt < 1:469397/71‑1 (MQ=255) gCCAGATCGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCACTATGa > 1:1765837/1‑58 (MQ=255) cAGATCGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGCCACAAGATCAt > 1:1808781/1‑71 (MQ=255) aGATCGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCACTATg < 1:2670876/54‑1 (MQ=255) gATCGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTg > 1:4444760/1‑41 (MQ=38) gATCGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGCCACAAGATCAtt > 1:1216325/1‑70 (MQ=255) gATCGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGCCACAAGATCAtt > 1:2949056/1‑70 (MQ=255) gATCGTTCTCGATACAGAAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGCCACAAGa < 1:1422213/65‑1 (MQ=255) aTCGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTg < 1:3566827/40‑1 (MQ=38) aTCGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCACTATg < 1:4349899/52‑1 (MQ=255) aTCGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGcc < 1:3200892/58‑1 (MQ=255) aTCGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGCCACAAGATCATTga < 1:3996346/71‑1 (MQ=255) aTCGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGCCACAAGATCATTga < 1:2804750/71‑1 (MQ=255) tCGTTCTCGCTACCGCAACCACCGGTATGAACCAGAtt < 1:3501226/38‑1 (MQ=37) tCGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTg < 1:2016888/39‑1 (MQ=37) tCGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGCCACAAGATCAtt > 1:4243160/1‑68 (MQ=255) tCGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGCCACAAGATCATTgag < 1:1000192/71‑1 (MQ=255) tCGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGCCACAAGATCATTga < 1:2376663/70‑1 (MQ=255) cGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTg > 1:2003997/1‑38 (MQ=37) cGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTGGTGAGCACTATGAAGGCCACAAGATCAtt > 1:2631597/1‑67 (MQ=255) cGTTCTCGATACCGAAACCACCGGTATGAACCAGATTGGTGCGCACTATGaa > 1:3791563/1‑52 (MQ=255) gTTCTCGCTACCGCAACCACCGGTATGAACCAGAtt < 1:338291/36‑1 (MQ=37) ttCTCGCTACCGCAATCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGCcaca < 1:1249725/57‑1 (MQ=255) ctcGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAg > 1:1716473/1‑49 (MQ=39) | ACTGTAAAACGACAAGTCTGACATAAATGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGATACCGAAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGCCACAAGATCATTGAGATTGGTG > W3110S.gb/236032‑236169 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |