Predicted mutation
evidence seq id position mutation freq annotation gene description
RA W3110S.gb 236,107 A→C 88.8% E14A (GAA→GCA)  dnaQ → DNA polymerase III epsilon subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb236,1070AC88.8% 2436.2 / 142.1 761E14A (GAA→GCA) dnaQDNA polymerase III epsilon subunit
Reads supporting (aligned to +/- strand):  ref base A (44/42);  new base C (368/307);  total (412/349)
Fisher's exact test for biased strand distribution p-value = 5.68e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.58e-01

Only 100 of 784 total aligned reads displayed.

AAACGACAAGTCTGACATAAATGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGATACCGAAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGCCACAAGATCATTGAGATTGGTGCCGTTG  >  W3110S.gb/236038‑236175
                                                                     |                                                                    
 caactCAAGTCTGACATAAATGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGCTACCGcaa                                                                    >  1:4171402‑M2/6‑71 (MQ=255)
 caactCAAGTCTGACATAAATGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGCTACCGcaa                                                                    >  1:2589643‑M2/6‑71 (MQ=255)
    ctCAAGTCTGACATAAATGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGCTACCGCAacca                                                                 >  1:4557576‑M2/3‑71 (MQ=255)
      cAAGTCTGACATAAATGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGCTACCGCAaccacc                                                               >  1:915664/1‑71 (MQ=255)
      cAAGTCTGACATAAATGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGCTACCGCAaccacc                                                               >  1:447142/1‑71 (MQ=255)
       aaGTCTGACATAAATGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGCTACCGCAaccac                                                                <  1:3560532/69‑1 (MQ=255)
       aaGTCTGACATAAATGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGCTACCGCAACCACCg                                                              >  1:3996534/1‑71 (MQ=255)
       aaGTCTGACATAAATGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGCTACCGCAACCACCg                                                              >  1:3483901/1‑71 (MQ=255)
        aGTCTGACATAAATGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGCTACCGCAACCACCgg                                                             <  1:3182670/71‑1 (MQ=255)
        aGTCTGACATAAATGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGCTACCGCAACCACCgg                                                             <  1:3070800/71‑1 (MQ=255)
              aCATAAATGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGCTACCGCAACCACCGGTATg                                                         <  1:1032368/69‑1 (MQ=255)
              aCATAAATGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGa                                                        <  1:1217951/70‑1 (MQ=255)
              aCATAAATGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGATACCGAAACCACCGGTATg                                                         <  1:255170/69‑1 (MQ=255)
               cATAAATGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGAAc                                                      <  1:2388311/71‑1 (MQ=255)
                aTAAATGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGa                                                        <  1:3601088/68‑1 (MQ=255)
                 tAAATGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGAAc                                                      <  1:2116769/69‑1 (MQ=255)
                 tAAATGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGAACCa                                                    <  1:759508/71‑1 (MQ=255)
                 tAAATGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGAACCa                                                    <  1:3532796/71‑1 (MQ=255)
                  aaaTGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGAAc                                                      >  1:2932314/1‑68 (MQ=255)
                  aaaTGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGAAc                                                      >  1:1614789/1‑68 (MQ=255)
                  aaaTGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGAAc                                                      >  1:2190200/1‑68 (MQ=255)
                  aaaTGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGAAc                                                      >  1:1949808/1‑68 (MQ=255)
                  aaaTGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGATACCGAAACCACCGGTATGAAc                                                      >  1:4097257/1‑68 (MQ=255)
                   aaTGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGAAc                                                      >  1:2007156/1‑67 (MQ=255)
                   aaTGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGAACCAGa                                                  <  1:862380/71‑1 (MQ=255)
                    aTGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGAACCAGAt                                                 >  1:2882271/1‑71 (MQ=255)
                     tGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGAACCAGAtt                                                <  1:3210683/71‑1 (MQ=255)
                     tGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGAACCAGAtt                                                <  1:2333067/71‑1 (MQ=255)
                     tGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGAACCAGAtt                                                >  1:1087/1‑71 (MQ=255)
                     tGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTAGCTACCGCAACCACCGGTATGAACCAGAtt                                                <  1:427434/71‑1 (MQ=255)
                      gACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGATACCGAAac                                                                   >  1:3263829/1‑51 (MQ=255)
                       aCCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTg                                               <  1:146120/70‑1 (MQ=255)
                         cGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTGGt                                             >  1:822402/1‑70 (MQ=255)
                         cGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTGGTg                                            <  1:1969896/71‑1 (MQ=255)
                         cGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTGGTg                                            >  1:114213/1‑71 (MQ=255)
                          gCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGCTACCGCAaccac                                                                >  1:3682853/1‑50 (MQ=255)
                          gCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGCTACCGCAACCACCgg                                                             <  1:4317720/53‑1 (MQ=255)
                          gCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTGGTgc                                           >  1:3725963/1‑71 (MQ=255)
                           cTATGAGCACTGCAATTACACGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTGGTgcg                                          >  1:243957/1‑71 (MQ=255)
                             aTGAGCACTGCAATTACACGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCa                                        >  1:572887/1‑71 (MQ=255)
                             aTGAGCACTGCAATTACACGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCa                                        >  1:1520853/1‑71 (MQ=255)
                               gAGCACTGCAATTACACGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGaa                                                       >  1:3593776/1‑54 (MQ=255)
                               gAGCACTGCAATTACACGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCa                                        >  1:3963573/1‑69 (MQ=255)
                                   aCTGCAATTACACGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGAAc                                                      <  1:3642284/51‑1 (MQ=255)
                                   aCTGCAATTACACGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCACTATg                                   >  1:4205689/1‑70 (MQ=255)
                                   aCTGCAATTACACGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCACTATg                                   >  1:3324783/1‑70 (MQ=255)
                                   aCTGCAATTACACGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCACTATGa                                  <  1:1334294/71‑1 (MQ=255)
                                    cTGCAATTACACGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCACTATg                                   >  1:4039786/1‑69 (MQ=255)
                                     tGCAATTACACGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGAACCa                                                    <  1:4408550/51‑1 (MQ=255)
                                     tGCAATTACACGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTGGTgcg                                          <  1:2233610/61‑1 (MQ=255)
                                     tGCAATTACACGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCACTATg                                   <  1:207122/68‑1 (MQ=255)
                                      gCAATTACACGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCACTATGa                                  >  1:3290290/1‑68 (MQ=255)
                                      gCAATTACACGCCAGATCGTTCTCGATACCGAAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAg                                >  1:2036876/1‑70 (MQ=255)
                                       cAATTACACGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGc                              >  1:2746599/1‑71 (MQ=255)
                                       cAATTACACGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGc                              >  1:1784586/1‑71 (MQ=255)
                                       cAATTACACGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGc                              >  1:3831219/1‑71 (MQ=255)
                                         aTTACACGCCAGATCGTTCTCGCTACCGCAACCACCGGTAt                                                          <  1:534190/41‑1 (MQ=38)
                                          ttACACGCCAGATCGTTCTCGCTACCGCAACCACCGGt                                                            <  1:3126339/38‑1 (MQ=38)
                                          ttACACGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTgg                                              <  1:683799/52‑1 (MQ=255)
                                          ttACACGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCACTAt                                    <  1:1904474/62‑1 (MQ=255)
                                          ttACACGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGCcac                           <  1:968504/71‑1 (MQ=255)
                                           tACACGCCAGATCGTTCTCGATACCGAAACCACCGGTATGAACCAGATTGGTGCGCACTATGAATGCcac                           >  1:4528105/1‑70 (MQ=255)
                                           tACACGCCAGATCGTTCTCGATACCGAAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGCcac                           >  1:478450/1‑70 (MQ=255)
                                            acacGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTg                                               >  1:3382793/1‑49 (MQ=39)
                                            acacGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGCcac                           >  1:3902900/1‑69 (MQ=255)
                                              acGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCACTATGa                                  <  1:789718/60‑1 (MQ=255)
                                               cGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGCCACAAGAt                      <  1:4351707/71‑1 (MQ=255)
                                                gCCAGATCGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCACTATGa                                  >  1:1765837/1‑58 (MQ=255)
                                                gCCAGATCGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGCCACAAGAt                      >  1:2830711/1‑70 (MQ=255)
                                                  cAGATCGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGCCACAAGATCAt                   >  1:1808781/1‑71 (MQ=255)
                                                   aGATCGTTCTCGCTACCGCAACCACCGGTATGAACCa                                                    <  1:2145115/37‑1 (MQ=37)
                                                   aGATCGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCACTATg                                   <  1:2670876/54‑1 (MQ=255)
                                                   aGATCGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGCCACAAGATCAtt                  >  1:3790072/1‑71 (MQ=255)
                                                    gATCGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTg                                               >  1:4444760/1‑41 (MQ=38)
                                                    gATCGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTGGT‑‑GCACTATGAAGGCCACAAGATCAt                   >  1:3454691/1‑67 (MQ=255)
                                                    gATCGTTCTCGATACAGAAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGCCACAAGa                       <  1:1422213/65‑1 (MQ=255)
                                                     aTCGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCACt                                      <  1:1283717/49‑1 (MQ=39)
                                                     aTCGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCACt                                      <  1:230216/49‑1 (MQ=39)
                                                     aTCGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGCCACAAGATCAtt                  >  1:1196151/1‑69 (MQ=255)
                                                     aTCGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGCCACAAGATCAtt                  >  1:647713/1‑69 (MQ=255)
                                                     aTCGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGCCACAAGATCATTg                 <  1:2788969/70‑1 (MQ=255)
                                                      tCGTTCTCGCTACCGCCACCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGCCACAAGATCAt                   >  1:2966536/1‑67 (MQ=255)
                                                      tCGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGCCACAAGATCa                    >  1:4242297/1‑66 (MQ=255)
                                                      tCGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGCCACAAGATCATTgag               <  1:1000192/71‑1 (MQ=255)
                                                       cGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGCCACAAGATCa                    >  1:2848634/1‑65 (MQ=255)
                                                       cGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGCCACAAGATCATTg                 >  1:3880240/1‑68 (MQ=255)
                                                       cGTTCTCGATACCGAAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGc                              >  1:734423/1‑55 (MQ=255)
                                                        gTTCTCGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGCCACAAGATCATTgaga              <  1:1552715/70‑1 (MQ=255)
                                                        gTTCTCGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGCCACAAGATCATTGAGAt             <  1:60230/71‑1 (MQ=255)
                                                         ttCTCGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGCCACAAGATCATTgaga              >  1:157840/1‑69 (MQ=255)
                                                         ttCTCGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGCCACAAGATCATTGAGAt             <  1:4075686/70‑1 (MQ=255)
                                                          tctcGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGCCACAAGATCATTGAGATTg           >  1:2623440/1‑71 (MQ=255)
                                                          tctcGATACCGAAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGCCACaa                         >  1:2520563/1‑57 (MQ=255)
                                                           ctcGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAg                                >  1:1716473/1‑49 (MQ=39)
                                                           ctcGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGCCACAAGATCATTGAGATTg           >  1:1385620/1‑70 (MQ=255)
                                                           ctcGATACCGAAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGCCACAAGATCATTGAGATTgg          >  1:2476782/1‑71 (MQ=255)
                                                            tcGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCAc                                       <  1:1657598/41‑1 (MQ=38)
                                                                tACCGCAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGCCACAAGATCATTGAGATTGGTGCCg     >  1:3400005/1‑71 (MQ=255)
                                                                  ccGCAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGCCACAAGATCATTGAGATTGGTGCCGtt   >  1:2710267/1‑71 (MQ=255)
                                                                  ccGCAACCACCGGTATGAACCAGATTGGTCCGCACTATGAAGGCCACAAGATCATTGAGATTGGTGCCGt    <  1:4147665/70‑1 (MQ=255)
                                                                     |                                                                    
AAACGACAAGTCTGACATAAATGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGATACCGAAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGCCACAAGATCATTGAGATTGGTGCCGTTG  >  W3110S.gb/236038‑236175

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: