| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 70,581 | C→T | 87.9% | V65V (GTC→GTT) | araC → | DNA‑binding transcriptional dual regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 70,581 | 0 | C | T | 87.9% | 805.7 / 85.1 | 306 | V65V (GTC→GTT) | araC | DNA‑binding transcriptional dual regulator |
| Reads supporting (aligned to +/- strand): ref base C (22/15); new base T (143/126); total (165/141) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.88e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 320 total aligned reads displayed.
GAAAGGTTATATTCTCAATCTCACCATTCGCGGTCAGGGGGTGGTGAAAAATCAGGGACGAGAATTTGTCTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGATTCATCACTACGGTCGTCATCCGGAG > W3110S.gb/70512‑70647 | aaaGGTTATATTCTCAATCTCACCATTCGCGGTCAGGGGGTGGTGAAAAATCAGGGACGAGAAtttgtttg > 1:4580011/1‑71 (MQ=255) aaaGGTTATATTCTCAATCTCACCATTCGCGGTCAGGGGGTGGTGAAAAATCAGGGACGAGAAtttgtttg > 1:2527583/1‑71 (MQ=255) ggTTATATTCTCAATCTCACCATTCGCGGTCAGGGGGTGGTGAAAAATCAGGGACGAGAATTTGTTTGCCg < 1:4060322/71‑1 (MQ=255) ttATATTCTCAATCTCACCATTCGCGGTCAGGGGGTGGTGAAAAATCAGGGACGAGAATTTGTTTGCCGAc > 1:2116346/1‑71 (MQ=255) ttATATTCTCAATCTCACCATTCGCGGTCAGGGGGTGGTGAAAAATCAGGGACGAGAATTTGTTTGCCGAc > 1:3281138/1‑71 (MQ=255) atatTCTCAATCTCACCATTCGCGGTCAGGGGGTGGTGAAAAATCAGGGACGAGAATTTGTTTGCCGAc > 1:4236593/1‑69 (MQ=255) atTCTCAATCTCACCATTCGCGGTCAGGGGGTGGTGAAAAATCAGGGACGAGAATTTGTTTGCCGACCgg < 1:2206045/70‑1 (MQ=255) atTCTCAATCTCACCATTCGCGGTCAGGGGGTGGTGAAAAATCAGGGACGAGAATTTGTTTACCGACCggg > 1:4303813/1‑71 (MQ=255) ttCTCAATCTCACCATTCGCGGTCAGGGGGTGGTGAAAAATCAGGGACGAGAATTTGTTTGCCGACCggg > 1:2107215/1‑70 (MQ=255) ttCTCAATCTCACCATTCGCGGTCAGGGGGTGGTGAAAAATCAGGGACGAGAATTTGTTTGCCGACCGGGt > 1:4144796/1‑71 (MQ=255) tctcAATCTCAGCATTCGCGGTCAGGGGTTGGTGAAAAATCAGGGACGAGAATTTGTTTGCCGACCgtgt < 1:4163482/70‑1 (MQ=255) ctcAATCTCCCCATTCGCGGTCAGGGGGTGGTGAAAAATCAGGGACGAGAATTTGTTTGCCGACCGGGTGa > 1:1689641/1‑71 (MQ=255) ctcAATCTCACCATTCGCGGTCAGGGGGTGGTGAAAAATCAGGGACGAGAATTTGTTTGCCGACCGGGTGa > 1:2294366/1‑71 (MQ=255) ctcAATCTCACCATTCGCGGTCAGGGGGTGGTGAAAAATCAGGGACGAGAATTTGTCTGCCGACCGGGTg < 1:224027/70‑1 (MQ=255) tcAATCTCACCATTCGCGGTCAGGGGGTGGTGAAAAATCAGGGACGAGAATTTGTTTGCCGACCGGGTGa > 1:1051766/1‑70 (MQ=255) tcAATCTCACCATTCGCGGTCAGGGGGTGGTGAAAAATCAGGGACGAGAATTTGTTTGCCGACCGGGTGa > 1:3143260/1‑70 (MQ=255) cAATCTCACCATTCGCGGTCAGGGGGTGGTGAAAAATCAGGGACGAGAATTTGTTTGCCGACCGGGTGata > 1:1021988/1‑71 (MQ=255) aaTCTCACCATTCGCGGTCAGGGGGTGGTGAAAAATCAGGGACGAGAATTTGTTTGCCGACCGGGTGatat > 1:1714435/1‑71 (MQ=255) tctcACCATTCGCGGTCAGGGGGTGGTGAAAAATCAGGGACGAGAATTTGTTTGCCGACCGGGTGATAttt > 1:2692816/1‑71 (MQ=255) tcACCATTCGCGGTCAGGGGGTGGTGAAAAATCAGGGACGAGAATTTGTTTGCCGACCGGGTGATATTTTg > 1:3081772/1‑71 (MQ=255) tcACCATTCGCGGTCAGGGGGTGGTGAAAAATCAGGGACGAGAATTTGTTTGCCGACCGGGTGATATTTTg > 1:4552685/1‑71 (MQ=255) aCCATTCGCGGTCAGGGGGTGGTGAAAAATCAGGGACGAGAATTTGTTTGCCGACCGGGTGATAttt < 1:4271515/67‑1 (MQ=255) aCCATTCGCGGTCAGGGGGTGGTGAAAAATCAGGGACGAGAATTTGTTTGCCGACCGGGTGATAttt < 1:192296/67‑1 (MQ=255) aCCATTCGCGGTCAGGGGGTGGTGAAAAATCAGGGACGAGAATTTGTTTGCCGACCGGGTGATATTTTGc < 1:3785598/70‑1 (MQ=255) aCCATTCGCGGTCAGGGGGTGGTGAAAAATCAGGGACGAGAATTTGTTTGCCGACCGGGTGATATTTTGCt < 1:3713722/71‑1 (MQ=255) aCCATTCGCGGTCAGGGGGTGGTGAAAAATCAGGGACGAGAATTTGTTTGCCGACCGGGTGATATTTTGCt < 1:3883811/71‑1 (MQ=255) aCCATTCGCGGTCAGGGGGTGGTGAAAAATCAGGGACGAGAATTTGTTTGCCGACCGGGTGATATTTTGCt < 1:842404/71‑1 (MQ=255) aCCATTCGCGGTCAGGGGGTGGTGAAAAATCAGGGACGAGAATTTGTTTGCCGACCGGGGGATATTTTg < 1:1993784/69‑1 (MQ=255) aCCATTCGCGGTCAGGGGGTGGTGAAAAATCAGGGACGAGAATTTGTCTGCCGACCGGGTGATAttt < 1:494936/67‑1 (MQ=255) aTTCGCGGTCAGGGGGTGGTGAAAAATCAGGGACGAGAATTTGTTTGCCGACCGGGTGATATTTTGCTGtt > 1:975109/1‑71 (MQ=255) aTTCGCGGTCAGGGGGTGGTGAAAAATCAGGGACGAGAATTTGTTTGCCGACCGGGTGATATTTTGCTGtt > 1:1147335/1‑71 (MQ=255) aTTCGCGGTCAGGGGGTGGTGAAAAATCAGGGACGAGAATTTGTTTGCCGACCGGGTGATATTTTGCTGtt < 1:1638910/71‑1 (MQ=255) ttCGCGGTCAGGGGGTGGTGAAAAATCAGGGACGAGAATTTGTTTGCCGACCGGGTGATATTTTGCTGTTc > 1:3533578/1‑71 (MQ=255) ttCGCGGTCAGGGGGTGGTGAAAAATCAGGGACGAGAATTTGTCTGCCGACCGGGTGATATTTTGCTGTTc > 1:959714/1‑71 (MQ=255) tCGCGGTCAGGGGGTGGTGAAAAATCAGGGACGAGAATTTGTTTGCCGACCGGGTGa < 1:1248188/57‑1 (MQ=255) gTCAGGGGGTGGTGAAAAATCAGGGACGAGAATTTGTTTGCCGACCGGGTg < 1:4377300/51‑1 (MQ=255) gTCAGGGGGTGGTGAAAAATCAGGGACGAGAATTTGTTTGCCGACCGGGTGATATTTTGCTGTTCCCGc > 1:1590943/1‑69 (MQ=255) gTCAGGGGGTGGTGAAAAATCAGGGACGAGAATTTGTTTGCCGACCGGGTGATATTTTGCTGTTCCCGc > 1:3661608/1‑69 (MQ=255) tCAGGGGGTGGTGAAAAATCAGGGACGAGAAtttgtttg < 1:788906/39‑1 (MQ=255) tCAGGGGGTGGTGAAAAATCAGGGACGAGAATTTGTTTGCCGACCGGGTGATATTTTGCTGtt < 1:1143160/63‑1 (MQ=255) tCAGGGGGTGGTGAAAAATCAGGGACGAGAATTTGTTTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAg > 1:639641/1‑71 (MQ=255) cAGGGGGTGGTGAAAAATCAGGGACGAGAATTTGTTTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAg > 1:3503595/1‑70 (MQ=255) aGGGGGTGGTGAAAAATCAGGGACGAGAATTTGTTTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAg > 1:3015004/1‑69 (MQ=255) aGGGGGTGGTGAAAAATCAGGGACGAGAATTTGTCTGCCGACCGGGTGATATTTTGc > 1:4595619/1‑57 (MQ=255) ggggTGGTGAAAAATCAGGGACGAGAATTTGTTTGCCg < 1:3061667/38‑1 (MQ=255) ggggTGGTGAAAAATCAGGGACGAGAATTTGTTTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGgaga < 1:2983979/71‑1 (MQ=255) gggTGGTGAAAAATCAGGGACGAGAATTTGTTTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGgagag < 1:2581451/71‑1 (MQ=255) gggTGGTGAAAAATCAGGGACGAGAATTTGTTTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGga > 1:2325714/1‑68 (MQ=255) ggtggtGAAAAATCAGGGACGAGAATTTGTTTGCCGAcc < 1:1546442/39‑1 (MQ=255) gtggtgAAAAATCAGGGACGAGAATTTGTTTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAgg < 1:3211833/65‑1 (MQ=255) gtggtgAAAAATCAGGGACGAGAATTTGTTTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGAt < 1:1256495/71‑1 (MQ=255) ggtgAAAAATCAGGGACGAGAATTTGTTTGCCGACCGGGTGatat < 1:2154468/45‑1 (MQ=255) gtgAAAAATCAGGGACGAGAATTTGTTTGCCGACCGGGTGATATTTTg > 1:4298584/1‑48 (MQ=255) gtgAAAAATCAGGGACGAGAATTTGTTTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGgagaga > 1:2779943/1‑67 (MQ=255) gtgAAAAATCAGGGACGAGAATTTGTTTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGATtca > 1:1736581/1‑71 (MQ=255) gtgAAAAATCAGGGACGAGAATTTGTTTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGATtc < 1:2227675/70‑1 (MQ=255) gtgAAAAATCAGGGACGAGAATTTGTCTGCCGACCGGGTGATATTTTGCTGTTCCCGc > 1:1301218/1‑58 (MQ=255) tgAAAAATCAGGGACGAGAATTTGTTTGCCGCCCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGATtca > 1:875330/1‑70 (MQ=255) tgAAAAATCAGGGACGAGAATTTGTTTGCCGACCGGGt < 1:1424852/38‑1 (MQ=255) tgAAAAATCAGGGACGAGAATTTGTTTGCCGACCGGGTGATATTTTGCTg < 1:4494261/50‑1 (MQ=255) tgAAAAATCAGGGACGAGAATTTGTTTGCCGACCGGGTGATATTTTGCTGTTCCTGc < 1:2389451/57‑1 (MQ=255) tgAAAAATCAGGGACGAGAATTTGTTTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGATtcat < 1:194359/71‑1 (MQ=255) tgAAAAATCAGGGACGAGAATTTGTTTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGATtcat < 1:255075/71‑1 (MQ=255) tgAAAAATCAGGGACGAGAATTTGTTTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGATtcat < 1:3265016/71‑1 (MQ=255) tgAAAAATCAGGGACGAGAATTTGTTTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGATtca < 1:2829588/70‑1 (MQ=255) gAAAAATCAGGGACGAGAATTTGTTTGCCGACCGGGTg > 1:569370/1‑38 (MQ=255) gAAAAATCAGGGACGAGAATTTGTTTGCCGACCGGGTGATATTTTGc > 1:1236132/1‑47 (MQ=255) gAAAAATCAGGGACGAGAATTTGTCTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGAt > 1:2413437/1‑66 (MQ=255) aaaaaTCAGGGACGAGAATTTGTCTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGATtcatca > 1:738997/1‑71 (MQ=255) aaaaaTCAGGGACGAGAATTTGTCTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGATtcatc > 1:892492/1‑70 (MQ=255) aaaaTCAGGGACGAGAATTTGTTTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGATTCATCAc < 1:807925/71‑1 (MQ=255) aaaTCAGGGACGAGAATTTGTTTGCCGACCGGGTGATATTTTGCTGTTCCCGc < 1:1698376/53‑1 (MQ=255) aaaTCAGGGACGAGAATTTGTTTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGATtca > 1:2060200/1‑66 (MQ=255) aaTCAGGGACGAGAATTTGTTTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAg > 1:33845/1‑55 (MQ=255) aaTCAGGGACGAGAATTTGTCTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGATtca < 1:407461/65‑1 (MQ=255) tCAGGGACGAGAATTTGTTTGCCGGCCGGGTGATATTTTg > 1:3853128/1‑40 (MQ=38) tCAGGGACGAGAATTTGTTTGCCGACCGGGTGatat < 1:358408/36‑1 (MQ=255) aGGGACGAGAATTTGTTTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGATtcatca > 1:4023530/1‑64 (MQ=255) aGGGACGAGAATTTGTTTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGATTCATCACTACGgt > 1:1379619/1‑71 (MQ=255) aGGGACGAGAATTTGTTTGCCGACAGGGTGATATTTTGCTGTTCCCGCCAGGAGAGATTCATCACTACgg < 1:2655501/70‑1 (MQ=255) gggACGAGAATTTGTTTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAg < 1:1892958/50‑1 (MQ=255) gggACGAGAATTTGTTTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGATTCATCACTACGgtc < 1:544016/71‑1 (MQ=255) cGAGAATTTGTTTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAg < 1:915589/46‑1 (MQ=255) cGAGAATTTGTTTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGATtc > 1:2928026/1‑55 (MQ=255) cGAGAATTTGTTTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGATTCTTCACTACGGTCGTCa > 1:1848019/1‑71 (MQ=255) gagaATTTGTTTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGATTCATCACTACGGTCGTCAt < 1:4464210/71‑1 (MQ=255) gagaATTTGTTTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGATTCATCACTACGGTCGTCAt > 1:698091/1‑71 (MQ=255) gagaATTTGTCTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAg > 1:1266124/1‑45 (MQ=255) agaATTTGTTTGCCGACCGGGTGATATTTTGCTGTTCCCGcc < 1:2187414/42‑1 (MQ=255) agaATTTGTCTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGATTCATCACTACGGTCGTCATc > 1:2068340/1‑71 (MQ=255) gaATTTGTTTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGgagaga > 1:648965/1‑49 (MQ=255) gaATTTGTTTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGATTCATCACTACAGTCGTCATcc > 1:1799713/1‑71 (MQ=255) aTTTGTTTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGATTCATCACTACGGTCGTCATCCgg < 1:4342961/71‑1 (MQ=255) aTTTGTTTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGATTCATCACTACGGTCGTCATCCg > 1:3357494/1‑70 (MQ=255) aTTTGTTTGCCGACCGGGTGATATTTTACTGTTCCCGCCAGGAGAGATTCATCACTACGGTCGTCATCCgg < 1:2493990/71‑1 (MQ=255) tttgtttgCCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGATTCATCAc > 1:1504790/1‑54 (MQ=255) tttgtttgCCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGATTCATCACTACGGTCGTCATCCg > 1:1164326/1‑69 (MQ=255) ttGTTTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGgaga > 1:3306260/1‑43 (MQ=255) ttGTTTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGATtcat > 1:3429928/1‑50 (MQ=255) tGTTTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGATTCATCACTACGGTCGTCATCCGGAg < 1:3486372/70‑1 (MQ=255) | GAAAGGTTATATTCTCAATCTCACCATTCGCGGTCAGGGGGTGGTGAAAAATCAGGGACGAGAATTTGTCTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGATTCATCACTACGGTCGTCATCCGGAG > W3110S.gb/70512‑70647 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |