| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 1,342,709 | G→A | 7.4% | Q146Q (CAG→CAA) | yciM → | conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 1,342,709 | 0 | G | A | 7.4% | 169.5 / 2.9 | 54 | Q146Q (CAG→CAA) | yciM | conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base G (21/29); new base A (2/2); total (23/31) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.54e-01 | |||||||||||
CGCGGAAGACATGTTCAATCAGCTGACCGATGAAACTGACTTCCGCATTGGCGCGCTGCAACAGTTGCTACAAATCTACCAGGCTACCAGTGAGTGGCAGAAAGCAATTGATGTTGCCGAACGCCTG > W3110S.gb/1342646‑1342772 | cgcgGAAGACATGTTCAATCAGCTGACCGATGAAACTGACTTCCGCATTGGCGCGCTGCAACAGTTGCTa > 1:34090/1‑70 (MQ=255) gAAGACATGTTCAATCAGCTGACCGATGAAACTGACTTCCGCATTGGCGCGCTGCAACAg < 1:2434747/60‑1 (MQ=255) aaGACATGTTCAATCAGCTGACCGATGAAACTGACTTCCGCATTGGCGCGCTGCAACAGTTGCTACAAATc > 1:2090437/1‑71 (MQ=255) aGACATGTTCAATCAGCTGACCGATGAAACTGACTTCCGCATTGGCGCGCTGCAACAGTTGCTACAAAt > 1:3895841/1‑69 (MQ=255) aGACATGTTCAATCAGCTGACCGATGAAACTGACTTCCGCATTGGCGCGCTGCAACAGTTGCTACAAATCt < 1:141897/71‑1 (MQ=255) aGACATGTTCAATCAGCTGACCGATGAAACTGACTTCCGCATTGGCGCGCTGCAACAGTTGCTACAAATCt < 1:2823892/71‑1 (MQ=255) aCATGTTCAATCAGCTGACCGATGAAACTGACTTCCGCATTGGCGCGCTGCAACAGTTGCTACAAATCTAc > 1:4324385/1‑71 (MQ=255) tGTTCAATCAGCTGACCGATGAAACTGACTTCCGCATTGGCGCGCTGCAACAGTTGCTACAAATCTACCa < 1:896241/70‑1 (MQ=255) tGTTCAATCAGCTGACCGATGAAACTGACTTCCGCATTGGCGCGCTGCAACAGTTGCTACAAATCTACCAg < 1:3189471/71‑1 (MQ=255) tGTTCAATCAGCTGACCGATGAAACTGACTTCCGCATTGGCGCGCTGCAACAACTGCTACaaa < 1:896431/63‑1 (MQ=255) tGTTCAATCAGCTGACCGATGAAACTGACTTCCGCATTGGCGCGCTGCAACAACTGCTACaaa < 1:153579/63‑1 (MQ=255) gTTCAATCAGCTGACCGATGAAACTGACTTCCGCATTGGCGCGCTGCAACAGTTGCTACAAATCt < 1:2493414/65‑1 (MQ=255) cAATCAGCTGACCGATGAAACTGACTTCCGCATTGGCGCGCTGCAACAGTTGCTACAAATCTACCAGGCt > 1:807238/1‑70 (MQ=255) aaTCAGCTGACCGATGAAACTGACTTCCGCATTGGCGCGCTGCAACAGTTGCTACAAATCTACCAGGCTAc < 1:1520258/71‑1 (MQ=255) aTCAGCTGACCGATGAAACTGACTTCCGCATTGGCGCGCTGCAACAGTTGCTACAAATCTACCAGGCTAcc < 1:3320242/71‑1 (MQ=255) cAGCTGACCGATGAAACTGACTTCCGCATTGGCGCGCTGCGACAGTTGCTACAAATCTACCa > 1:76924/1‑62 (MQ=255) cAGCTGACCGATGAAACTGACTTCCGCATTGGCGCGCTGCAACAGtt > 1:1100043/1‑47 (MQ=255) aGCTGACCGATGAAACTGACTTCCGCATTGGCGCGCTGCAACAGTTGCTACAAATCTACCAGGCTACCagt > 1:2060513/1‑71 (MQ=255) aGCTGACCGATGAAACTGACTTCCGCATTGGCGCGCTGCAACAGTTGCTACAAATCTACCAGGCTACCag > 1:3719054/1‑70 (MQ=255) gCTGACCGATGAAACTGACTTCCGCATTGGCGCGCTGCAACAGTTGCTACAAATCt < 1:3739672/56‑1 (MQ=255) gCTGACCGATGAAACTGACTTCCGCATTGGCGCGCTGCAACAGTTGCTACAAATCTACCAGGCTACCagtg < 1:2695861/71‑1 (MQ=255) aCCGATGAAACTGACTTCCGCATTGGCGCGCTGCAACAGTTGCTACAAATCTACCAGGCTACCagtgagtg > 1:2421506/1‑71 (MQ=255) aCCGATGAAACTGACTTCCGCATTGGCGCGCTGCAACAGTTGCTACAAATCTACCAGGCTACCagtgagtg > 1:3409361/1‑71 (MQ=255) aCCGATGAAACTGACTTCCGCATTGGCGCGCTGCAACAACTGCTACAAAtt > 1:694467/1‑50 (MQ=255) aCCGATGAAACTGACATCCGCATTGGCGCGCTGCAACAACTGCTACAAAtt > 1:2091731/1‑50 (MQ=255) ccGATGAAACTGACTTCCGCATTGGCGCGCTGCAACAGTTGCTACAAATCTACCAGGCTACCAGTGAGTgg < 1:2975885/71‑1 (MQ=255) aTGAAACTGACTTCCGCATTGGCGCGCTGCAACAGTTGCTACa < 1:2144865/43‑1 (MQ=255) aTGAAACTGACTTCCGCATTGGCGCGCTGCAACAGTTGCTACa < 1:1689341/43‑1 (MQ=255) aTGAAACTGACTTCCGCATTGGCGCGCTGCAACAGTTGCTACAAATCTAc < 1:2609501/50‑1 (MQ=255) gAAACTGACTTCCGCATTGGCGCGCTGCAACAGTTGCTACAAATCTACCAGGCTACCAGTGAGTGGCAGa > 1:2903623/1‑70 (MQ=255) aCTGACTTCCGCATTGGCGCGCTGCAACAGTTGCTACAAATCTACCAGGCTACCAGTGAGTGGCAGAAAg > 1:4309314/1‑70 (MQ=255) aCTTCCGCATTGGCGCGCTGCAACAGTTGCTACAAATCTACCAGGCTACCAGTGAGTGGCAGAAAGCaa < 1:2636543/69‑1 (MQ=255) ccGCATTGGCGCGCTTCAACAGTTGCTACAAATCTACCAGGCTACCAGTGAGTGGCAGAAAGCAATTGa < 1:2352716/69‑1 (MQ=255) ccGCATTGGCGCGCTGCAACAGTTGCTACAAATCTACCAGGCTACCAGTGAGTGGCAGAAAGCAATTGa < 1:2830077/69‑1 (MQ=255) ccGCATTGGCGCGCTGCAACAGTTGCTACAAATCTACCAGGCTACCAGTGAGTGGCAGAAAGCAATTGATg > 1:3415498/1‑71 (MQ=255) cGCATTGGCGCGCTGCAACAGTTGCTACAAATCTACCAGGCTACCAGTGAGTGGCAGAAAGCaa > 1:1238857/1‑64 (MQ=255) cATTGGCGCGCTGCAACAGTTGCTACAAATCTACCAGGCTACCAGTGAGTGGCAGAAAGCAATTGATGTTg < 1:1722090/71‑1 (MQ=255) aTTGGCGCGCTGCAACAGTTGCTACAAATCTACCAGGCTACCAGTGAGTGGCAGAAAGCAAtt < 1:3029930/63‑1 (MQ=255) ttGGCGCGCTGCAACAGTTGCTACAAATCTACCAGGCTACCAGTGAGTGGCAGa < 1:184868/54‑1 (MQ=255) ttGGCGCGCTGCAACAGTTGCTACAAATCTACCAGGCTACCAGTGAGTGGCAGa < 1:190208/54‑1 (MQ=255) ttGGCGCGCTGCAACAGTTGCTACAAATCTACCAGGCTACCAGTGAGTGGCAGAAAGCAATTGATGTTGcc > 1:2639153/1‑71 (MQ=255) tGGCGCGCTGCAACAGTTGCTACAAATCTACCAGGCTACCAGTGAGTGGCAGAAAGCAATTGa > 1:3471133/1‑63 (MQ=255) ggCGCGCTGCAACAGTTGCTACAAATCTACCAGGCTACCAGTGAGTGGCAGAAAGCAATTGATGTTGCCg < 1:3532970/70‑1 (MQ=255) cgcgcTGCAACAGTTGCTACAAATCTACCAGGCTACCAGTGAGTGGCAGAAAGCAATTGATGTTGCCGaa > 1:2058406/1‑70 (MQ=255) gcgcTGCAACAGTTGCTACAAATCTACCAGGCTACCagtgag < 1:3423154/42‑1 (MQ=255) gcgcTGCAACAGTTGCTACAAATCTACCAGGCTACCagtgag < 1:134694/42‑1 (MQ=255) gcTGCAACAGTTGCTACAAATCTACCAGGCTACCAGTGAGTGGCAGAAAGCAATTGATGTTGCCGAACGcc > 1:51430/1‑71 (MQ=255) cTGCAACAGTTGCTACAAATCTACCAGGCTACCAGTGAGTGGCAGAAAGCAATTGAt < 1:2070543/57‑1 (MQ=255) tGCAACAGTTGCTACAAATCTACCAGGCTACCAGTGAGTGGCAGAAAGCAATTGAt < 1:4531701/56‑1 (MQ=255) gCAACAGTTGCTACAAATCTACCAGGCTACCAGTGAGTGGCAGAAAGCAATTGATGTTGCCGAAc < 1:2174230/65‑1 (MQ=255) cAACAGTTGCTACAAATCTACCAGGCTACCAGTGAGTGGCAGAAAGCAATTGATGTTGc > 1:3792040/1‑59 (MQ=255) cAACAGTTGCTACAAATCTACCAGGCTACCAGTGAGTGGCAGAAAGCAATTGATGTTGCCGAACGcc < 1:342410/67‑1 (MQ=255) cAACAGTTGCTACAAATCTACCAGGCTACCAGTGAGTGGCAGAAAGCAATTGATGTTGCCGAACGCCTg < 1:3174289/69‑1 (MQ=255) aCAGTTGCTACAAATCTACCAGGCTACCAGTGAGTGGCAGAAAGCAAt > 1:3651372/1‑48 (MQ=255) aCAGTTGCTACAAATCTA‑CAGGCTACCAGTGAGTGGCAGAAAGCAATTGATGTTGCCg < 1:1589237/58‑1 (MQ=255) | CGCGGAAGACATGTTCAATCAGCTGACCGATGAAACTGACTTCCGCATTGGCGCGCTGCAACAGTTGCTACAAATCTACCAGGCTACCAGTGAGTGGCAGAAAGCAATTGATGTTGCCGAACGCCTG > W3110S.gb/1342646‑1342772 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |