| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 71,175 | A→G | 86.2% | R263R (CGA→CGG) | araC → | DNA‑binding transcriptional dual regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 71,175 | 0 | A | G | 86.2% | 800.5 / 93.3 | 296 | R263R (CGA→CGG) | araC | DNA‑binding transcriptional dual regulator |
| Reads supporting (aligned to +/- strand): ref base A (26/15); new base G (93/162); total (119/177) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.74e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.35e-01 | |||||||||||
Only 100 of 298 total aligned reads displayed.
TACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGAGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTTCGTGCCGGTTGTGAAGAAAAAGTGAATG > W3110S.gb/71109‑71242 | caCCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTAt < 1:4412795/70‑1 (MQ=255) cccGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTAtt > 1:8503/1‑70 (MQ=255) cccGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTAtt > 1:1766545/1‑70 (MQ=255) cccGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGAGTAtt > 1:798121/1‑70 (MQ=255) cccGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGAGTAtt > 1:3176606/1‑70 (MQ=255) cGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTa > 1:1774357/1‑70 (MQ=255) cGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGAGTATTTaa < 1:4000321/71‑1 (MQ=255) ggATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTaaa < 1:2011780/71‑1 (MQ=255) ggATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTaa > 1:364457/1‑70 (MQ=255) ggATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTaa > 1:1495680/1‑70 (MQ=255) gATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTaaaa < 1:2212090/71‑1 (MQ=255) aTGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTaaaaa < 1:3626290/71‑1 (MQ=255) tGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGa > 1:3979999/1‑59 (MQ=255) tGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTaaaaaa < 1:3396810/71‑1 (MQ=255) gCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTAAAAAAt > 1:3451157/1‑71 (MQ=255) cTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGAGTATTTAAAAAATg > 1:373750/1‑70 (MQ=255) cTATAGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTaaaa < 1:1737590/66‑1 (MQ=255) tATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTAt < 1:468499/59‑1 (MQ=255) tATCGCCACCGTCGGT‑‑CAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGAGTATTTAAAAAATGCa > 1:459116/1‑69 (MQ=255) aTCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTAAAAAATGCAc > 1:4525798/1‑71 (MQ=255) aTCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTAAAAAATGCAc > 1:2070789/1‑71 (MQ=255) tCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTa > 1:4281884/1‑56 (MQ=255) tCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTAt > 1:2172146/1‑57 (MQ=255) tCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTAAAAAATGCAcc < 1:715875/71‑1 (MQ=255) tCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTAAAAAATGCAcc > 1:2496467/1‑71 (MQ=255) tCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTAAAAAATGCAcc < 1:770405/71‑1 (MQ=255) gCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTAAAAAATGCACCg < 1:2017638/70‑1 (MQ=255) aCCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTAAAAAATGCACCggg < 1:3310922/69‑1 (MQ=255) aCCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTAAAAAATGCACCGGGGc < 1:4482074/71‑1 (MQ=255) aCCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGAGTATTTAAAAAATGc > 1:1191487/1‑63 (MQ=255) ccGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTAttt > 1:436522/1‑53 (MQ=255) ccGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTAAAAAATGCACCGGGGc > 1:3441382/1‑70 (MQ=255) gtcggtcgCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTaaaaaa > 1:2106364/1‑57 (MQ=255) gtcggtcgCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTAAAAAATGCAc > 1:2987830/1‑62 (MQ=255) gtcggtcgCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTAAAAAATGCACCGGGGCCAg < 1:1619655/71‑1 (MQ=255) gtcggtcgCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTAAAAAATGCACCGGGGCCAg > 1:353647/1‑71 (MQ=255) tcggtcgCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTaaaaaa < 1:1361308/56‑1 (MQ=255) ggtcgCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTaaaa < 1:1262366/52‑1 (MQ=255) ggtcgCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTAAAAAATGCACCGGGGCCAGccc < 1:4312587/71‑1 (MQ=255) ggtcgCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTAAAAAATGCACCGGGGCCAGcc < 1:384736/70‑1 (MQ=255) gtcgCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTa < 1:1564231/44‑1 (MQ=255) gtcgCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTAAAAAATGCACCGGGGCCAGccc < 1:2550121/70‑1 (MQ=255) gtcgCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTAAAAAATGCACCGGGGCCAGCCcg < 1:3912613/71‑1 (MQ=255) gtcgCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTAAAAAATGCACCGGGGCCAGCCcg < 1:2911084/71‑1 (MQ=255) tcgCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGAGTATTTAAAAAATGCACCgggg < 1:1968539/62‑1 (MQ=255) cgCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGAGTATTTAAAAAATGCACCGGGGCCAGCCcgag > 1:1696269/1‑71 (MQ=255) gCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTaaaa > 1:1478275/1‑48 (MQ=255) gCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTAAAAAATGCACCGGGGCCAGCCcgagc < 1:3268933/71‑1 (MQ=255) cAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTAAAAAATGCACCGGGGCCAGCCcgagcg < 1:1229082/71‑1 (MQ=255) cAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTAAAAAATGCACCGGGGCCAGCCcgagcg < 1:1840504/71‑1 (MQ=255) cAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTAAAAAATGCACCGGGGCCAGCCcgagc > 1:2969849/1‑70 (MQ=255) cAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTAAAAAATGCACCGGGGCCAGCCcgag > 1:2346736/1‑69 (MQ=255) aTGTTGGTTTTTACGATCAACTCTATTTCTCGCGGGTATTTaaaaaa > 1:1797409/1‑47 (MQ=38) aTGTTGGTTTTGACGATTAACTCTATTTCTCGCGGGTATTTaa < 1:1150202/43‑1 (MQ=38) aTGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTaaaaaa < 1:690187/47‑1 (MQ=255) tGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTaaaaa > 1:1912171/1‑45 (MQ=255) tGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTaa < 1:2451573/42‑1 (MQ=255) gTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTaaaaaa > 1:284195/1‑45 (MQ=255) ttGGTTTTGACGATCAACTCTATTTCTCGCGGGTa < 1:3603183/35‑1 (MQ=255) ttGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTaaaa < 1:142154/42‑1 (MQ=255) ttGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTaaaa < 1:620653/42‑1 (MQ=255) ttGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTaa < 1:656911/40‑1 (MQ=255) ttGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTaa < 1:3210422/40‑1 (MQ=255) tGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGttc > 1:3076664/1‑69 (MQ=255) ggTTTTGACGATCAACTCTATTTCTCGCGGGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGttcc < 1:1311434/70‑3 (MQ=255) gTTTTGACGATCAACTCTATTTCTCGCGGGTATTTAAAAAATGCACCgg < 1:3464148/49‑1 (MQ=255) ttttGACGATCAACTCTATTTCTCGCGGGTATTTAAAAAATGc < 1:2737691/43‑1 (MQ=255) ttttGACGATCAACTCTATTTCTCGCGGGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTCCGTg < 1:4576905/71‑1 (MQ=255) tttGACGATCAACTCTATTTCTCGCGAGTATTTaaa < 1:1057919/36‑1 (MQ=255) ttGACGATCAACTCTATTTCTCGCGGGTATTTAAAAAATGCACCg < 1:3061994/45‑1 (MQ=255) ttGACGATCAACTCTATTTCTCGCGGGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTCCGTGc < 1:1652480/70‑1 (MQ=255) ttGACGATCAACTCTATTTCTCGCGAGTATTTaaaaaa > 1:3345248/1‑38 (MQ=255) tGACGATCAACTCTATTTCTCGCGGGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTCCGTGc < 1:140839/69‑1 (MQ=255) aCGATCAACTCTATTTCTCGCGGGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTCCGTGCCgg < 1:75419/70‑1 (MQ=255) cGATCAACTCTATTTCTCGCGGGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTCCGTGCCGGtt > 1:277176/1‑71 (MQ=255) cGATCAACTCTATTTCTCGCGAGTATTTAAAAAATGCACCGGGGCCAGCCcgagcgag > 1:2440084/1‑58 (MQ=255) aTCAACTCTATTTCTCGCGAGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTTCGTGcc > 1:3882219/1‑65 (MQ=255) tCAACTCTATTTCTCGCGGGTATTTAAAAAATGCACCg > 1:4232697/1‑38 (MQ=255) cAACTCTATTTCTCGCGGGTATTTAAAAAATGCACCGGGGCCAGCCcg < 1:2684622/48‑1 (MQ=255) cAACTCTATTTCTCGCGGGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGttcc < 1:4302288/58‑3 (MQ=255) cAACTCTATTTCTCGCGGGTATTTAAAAAATGCACCGGGCCCAGCCCGAGCGAGTTCCGTGCCGGTtgtg > 1:4114243/1‑70 (MQ=255) aCTCTATTTCTCGCGGGTATTTAAAAAATGCACCGGGGc < 1:3136965/39‑1 (MQ=255) ctctATTTCTCGCGGGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTCCGTGCCGGTTGTgaaga < 1:971361/71‑1 (MQ=255) ctctATTTCTCGCGGGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTCCGTGCCGGTTGTgaa > 1:2310111/1‑69 (MQ=255) aTTTCTCGCGGGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTCCGTGCCGGTTGTGAAGaaa > 1:511064/1‑69 (MQ=255) tttCTCGCGGGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGttc > 1:3303763/1‑48 (MQ=255) tttCTCGCGGGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTCCGTGCCGGTTGTgaa > 1:4555480/1‑64 (MQ=255) tttCTCGCGGGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTCCGTGCCGGTTGTGAAGaaaaa < 1:1921483/70‑1 (MQ=255) tttCTCGCGGGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTCCGTGCCGGTTGTGAAGaaaaa < 1:3770704/70‑1 (MQ=255) tttCTCGCGGGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTCCGTGCCGGTTGTGAAGaaa < 1:1879701/68‑1 (MQ=255) tttCTCGCGGGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTCCGTGCCGGTTGTGAAGaaa < 1:4041905/68‑1 (MQ=255) tttCTCGCGGGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTCCGTGCCGGTTGTGAAGaaa < 1:3566865/68‑1 (MQ=255) tttCTCGCGAGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTTCGTGCCGGTTGTGAAGaaa < 1:543315/68‑1 (MQ=255) tctcGCGGGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTCCGTGCCGGTTGTGAAGAAAAAGt > 1:936084/1‑70 (MQ=255) gcgGGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTCCGTGCCGGTTGTGAAGAAAAAGTGa < 1:3420447/68‑1 (MQ=255) gcgGGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTCCGTGCCGGTTGTGAAGAAAAAGTGAatg < 1:2427052/71‑1 (MQ=255) gcgGGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTCCGTGCCGGTTGTGAAGAAAAAGTGAatg < 1:2917194/71‑1 (MQ=255) gcgGGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTCCGTGCCGGTTGTGAAGAAAAAGTGAatg < 1:4208536/71‑1 (MQ=255) gcgGGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTCCGTGCCGGTTGTGAAGAAAAAGTGAatg < 1:1533707/71‑1 (MQ=255) gAGTATTTAAAAAATGCACCGGGGCCAGCCcgagcga > 1:1029154/1‑37 (MQ=255) | TACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGAGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTTCGTGCCGGTTGTGAAGAAAAAGTGAATG > W3110S.gb/71109‑71242 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |