| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 71,214 | T→C | 88.4% | F276F (TTT→TTC) | araC → | DNA‑binding transcriptional dual regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 71,214 | 0 | T | C | 88.4% | 770.7 / 66.8 | 267 | F276F (TTT→TTC) | araC | DNA‑binding transcriptional dual regulator |
| Reads supporting (aligned to +/- strand): ref base T (23/8); new base C (109/126); total (133/134) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.98e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.53e-01 | |||||||||||
Only 100 of 274 total aligned reads displayed.
TTGGTTTTGACGATCAACTCTATTTCTCGCGAGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTTCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTCAAGTTGTCATAATTGGTAACGAATCAGACAA > W3110S.gb/71144‑71284 | ttGGTTTTGACGATCAACTCTATTTCTCGCGAGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGttt > 1:4588411/1‑71 (MQ=255)ttGGTTTTGACGATCAACTCTATTTCTCGCGAGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGttt > 1:4307411/1‑71 (MQ=255) gTTTTGACGATCAACTCTATTTCTCGCGAGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTTCGt < 1:2985880/71‑1 (MQ=255) ttGACGATCAACTCTATTTCTCGCGGGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTCCGTGc < 1:1652480/70‑1 (MQ=255) ttGACGATCAACTCTATTTCTCGCGAGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTTCGTGcc > 1:3629907/1‑71 (MQ=255) tGACGATCAACTCTATTTCTCGCGGGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTCCGTGCCg > 1:3606947/1‑71 (MQ=255) cGATCAACTCTATTTCTCGCGGGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTCCGTGCCGGtt > 1:277176/1‑71 (MQ=255) tCAACTCTATTTCTCGCGGGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTCCGTGCCGGTtgtg < 1:1915228/71‑1 (MQ=255) cAACTCTATTTCTCGCGGGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTCCGTGCCGGTtgtg < 1:1940204/70‑1 (MQ=255) cAACTCTATTTCTCGCGGGTATTTAAAAAATGCACCGGGCCCAGCCCGAGCGAGTTCCGTGCCGGTtgtg > 1:4114243/1‑70 (MQ=255) ctctATTTCTCGCGGGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTCCGTGCCGGTTGTgaag > 1:4001366/1‑70 (MQ=255) tctATTTCTCGCGGGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTCCGTGCCGGTTGTgaaga > 1:947741/1‑70 (MQ=255) ctATTTCTCGCGGGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTCCGTGCCGGTTGTgaagaa > 1:2615798/1‑70 (MQ=255) ctATTTCTCGCGGGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTCCGTGCCGGTTGTgaagaa > 1:805386/1‑70 (MQ=255) aTTTCTCGCGGGTATTTAAAAAATGCACC‑GGGCCAGCCCGAGCGAGTTCCGTGCCGGTTGTGAAGAAAAAg > 1:4098091/1‑71 (MQ=255) tttCTCGCGGGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTCCGTGCCGGTTGTgaa > 1:4555480/1‑64 (MQ=255) tttCTCGCGGGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTCCGTGCCGGTTGTGAAGaaaaa < 1:3770704/70‑1 (MQ=255) tttCTCGCGGGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTCCGTGCCGGTTGTGAAGaaa < 1:4041905/68‑1 (MQ=255) tttCTCGCGGGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTCCGTGCCGGTTGTGAAGaaa < 1:1306076/68‑1 (MQ=255) tttCTCGCGGGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTCCGTGCCGGTTGTGAAGaaa < 1:3566865/68‑1 (MQ=255) tttCTCGCGGGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTCCGTGCCGGTTGTGAAGaaa < 1:582618/68‑1 (MQ=255) tttCTCGCGGGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTCCGTGCCGGTTGTGAAGaaa < 1:1879701/68‑1 (MQ=255) tttCTCGCGGGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTCCGTGCCGGTTGTGAAGAAAAAg > 1:3273494/1‑71 (MQ=255) tttCTCGCGAGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTTCGTGCCGGTTGTGAAGaaa < 1:543315/68‑1 (MQ=255) tctcGCGGGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTCCGTGCCGGTTGTGAAGAAAAAGt < 1:3450029/70‑1 (MQ=255) ctcGCGGGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTCCGTGCCGGTTGTGAAGAAAAAGTg < 1:3414075/70‑1 (MQ=255) tcGCGAGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTTCGTGCCGGTTGTGAAGaaaaa > 1:1596422/1‑66 (MQ=255) cgcgGGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTCCGTGCCGGTTGTGAAGAAAAAGTGAat > 1:2308888/1‑71 (MQ=255) gcgGGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTCCGTGCCGGTTGTGAAGAAAAAGTGa < 1:3420447/68‑1 (MQ=255) gcgGGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTCCGTGCCGGTTGTGAAGAAAAAGTGAatg < 1:1533707/71‑1 (MQ=255) gcgGGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTCCGTGCCGGTTGTGAAGAAAAAGTGAatg < 1:2917194/71‑1 (MQ=255) gcgGGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTCCGTGCCGGTTGTGAAGAAAAAGTGAatg < 1:4208536/71‑1 (MQ=255) gcgGGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTCCGTGCCGGTTGTGAAGAAAAAGTGAatg < 1:2427052/71‑1 (MQ=255) gcgGGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTCCGTGCCGGTTGTGAAGAAAAAGTGAat < 1:1218149/70‑1 (MQ=255) gggTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTCCGTGCCGGTTGTGAAGAAAAAGTGAat < 1:274370/66‑1 (MQ=255) gTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTCCGTGCCGGTTGTgaag < 1:1013154/55‑1 (MQ=255) gTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTCCGTGCCGGTTGTGAAGaaa < 1:1172135/58‑1 (MQ=255) aTTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTCCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAg > 1:4328539/1‑71 (MQ=255) aTTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTCCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAg > 1:1038102/1‑71 (MQ=255) tttAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTCCGTGCCGGtt < 1:3054026/46‑1 (MQ=255) tttAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTCCGTGCCGGTtgtg < 1:1369722/49‑1 (MQ=255) tttAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTCCGTGCCGGTTGTGAAGAAAAAGt < 1:2592728/59‑1 (MQ=255) tttAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTCCGTGCCGGTTGTGAAGAAAAAGTGaa < 1:1251663/62‑1 (MQ=255) ttAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTCCGTGCCGGTTGTGAAGAAAAAg > 1:2381429/1‑57 (MQ=255) tAAAAAATTCACCGGGGCCAGCCCGAGCGAGTTTCGTGCCGGTTGTGAAGaaaaa > 1:2252816/1‑55 (MQ=255) tAAAAAATGCACCGGGGCCAGCCCGAGCGAGATCCGTGCCg > 1:2123958/1‑41 (MQ=255) tAAAAAATGCACCCGGGCCAGCCCGAGCGAGTTCCGTGCCGGTTGTGAAGaaaa > 1:4069015/1‑54 (MQ=255) aaaaaaTGCACCGGGGCCAGCCCGAGCGAGTTCCGTGCCGGTTGTGAAGAAAAAGTGAatgatg < 1:379233/64‑1 (MQ=255) aaaaaaTGCACCGGGGCCAGCCCGAGCGAGTTCCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGt > 1:2844904/1‑71 (MQ=255) aaaaaaTGCACCGGGGCCAGCCCGAGAGAGTTCCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGt > 1:2030947/1‑71 (MQ=255) aaaaaTGCACCGGGGCCAGCCCGAGCGAGTTTCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGt > 1:3951357/1‑70 (MQ=255) aaaaaTGCACCGGGGCCAGCCCGAGCGAGTTCCGTGCCGGTTGTgaag > 1:1522584/1‑48 (MQ=255) aaaaTGCACCGGGGCCAGCCCGAGCGAGTTCCGTGCCGGTTGTGAAGaaa < 1:1636634/50‑1 (MQ=255) aaaTGCACCGGGGCCAGCCCGAGCGAGTTCCGTGCCGGTTGTgaa > 1:3706592/1‑45 (MQ=255) aaaTGCACCGGGGCCAGCCCGAGCGAGTTCCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTc > 1:2217531/1‑69 (MQ=255) tGCACCGGGGCCAGCCCGAGCGAGTTCCGTGCCGGTTGTGAAGAAAAAGTGAAAGATGTAGCCGTCAAGtt < 1:2367746/71‑1 (MQ=255) cACCGGGGCCAGCCCGAGCGAGTTCCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTCAAGTTg < 1:1986693/70‑1 (MQ=255) cACCGGGGCCAGCCCGAGCGAGTTCCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTCAAGTTGt < 1:3838709/71‑1 (MQ=255) aCCGGGGCCAGCCCGAGCGAGTTCCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTc < 1:1109090/63‑1 (MQ=255) ccGGGGCCAGCCCGAGCGAGTTCCGTGCCGGTTGTgaaga < 1:1834257/40‑1 (MQ=255) ccGGGGCCAGCCCGAGCGAGTTCCGTGCCGGTTGTGAAGAAAAAGTGAatga < 1:1000136/52‑1 (MQ=255) cGGGGCCAGCCCGAGCGAGTTTCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTCAAGTTGTCa > 1:3515115/1‑70 (MQ=255) cGGGGCCAGCCCGAGCGAGTTCCGTGCCGGTTGTGAAGAAAAAGTGAat < 1:4364438/49‑1 (MQ=255) cGGGGCCAGCCCGAGCGAGTTCCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTCAAGTTGTCa > 1:3335957/1‑70 (MQ=255) cGGGGCCAGCCCGAGCGAGTTCCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTCAAGTTGTCa > 1:4568800/1‑70 (MQ=255) cGGGGCCAGCCCGAGCGAGTTCCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTCAAGTTGTCa > 1:1787508/1‑70 (MQ=255) cGGGGCCAGCCCGAGCGAGTTCCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTCAAGTTGTCAt < 1:6644/71‑1 (MQ=255) ggggCCAGCCCGAGCGAGTTCCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTCAAGTTGTCATa < 1:1084731/71‑1 (MQ=255) gggCCAGCCCGAGCGAGTTCCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTCaa > 1:2544374/1‑61 (MQ=255) gggCCAGCCCGAGCGAGTTCCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTCAAGTTGTCATaa < 1:1232123/71‑1 (MQ=255) gggCCAGCCCGAGCGAGTTCCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTCAAGTTGTCATa > 1:3538859/1‑70 (MQ=255) gggCCAGCCCGAGCGAGTTCCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTCAAGTTGTCATa < 1:3680454/70‑1 (MQ=255) gggCCAGCCCGAGCGAGTTCCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTCAAGTTGTCATa > 1:612790/1‑70 (MQ=255) gggCCAGCCCGAGCGAGTTCCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTCAAGTTGTCATa > 1:504386/1‑70 (MQ=255) ggCCAGCCCGAGCGAGTTCCGTGCCGGTTGTGAAGAAAAAGTg < 1:4432870/43‑1 (MQ=255) ggCCAGCCCGAGCGAGTTCCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTCAAGTTGTCATa > 1:3123663/1‑69 (MQ=255) ccAGCCCGAGCGAGTTCCGTGCCGGTTGTGAAGAAAAAGTGaa > 1:525818/1‑43 (MQ=255) cAGCCCGAGCGAGTTTCGTGCCGGTTGTGAAGAAAAAGTGAat < 1:869121/43‑1 (MQ=255) cAGCCCGAGCGAGTTCCGTGCCGGTTGTGAAGAAAAAGTGAatgat > 1:3901037/1‑46 (MQ=255) aGCCCGAGCGAGTTCCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTCAAGTTGTCATAAagggt > 1:385277‑M1/1‑66 (MQ=255) cccGAGCGAGTTCCGTGCCGGTTGTGAAGAAAAAGTGAat > 1:416116/1‑40 (MQ=255) cccGAGCGAGTTCCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGc > 1:2090504/1‑48 (MQ=255) ccGAGCGAGTTCCGTGCCGGTTGTGAAGAAAAAGTGaa > 1:82627/1‑38 (MQ=255) ccGAGCGAGTTCCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTCaa < 1:4503195/53‑1 (MQ=255) ccGAGCGAGTTCCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTCAAGTTGTCATAAagggtccc < 1:1469015‑M1/71‑9 (MQ=255) ccGAGCGAGTTCCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTCAAGTTGTCATAAagggtccc > 1:746117‑M1/1‑63 (MQ=255) cgagcgagTTCCGTGCCGGTTGTGAAGAAAAAGTGAatgat > 1:1730573/1‑41 (MQ=255) cgagcgagTTCCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTCaa < 1:4345656/52‑1 (MQ=255) cgagcgagTTCCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTCAAGTTGTCATAAagggtccct < 1:2678823‑M1/71‑10 (MQ=255) cgagcgagTTCCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTCAAGTTGTCATAAagggtccct < 1:1451588‑M1/71‑10 (MQ=255) cgagcgagTTCCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTCAAGTTGTCATAAagggtccct < 1:2924764‑M1/71‑10 (MQ=255) gagcgagTTCCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTCAAGTTGTCATAAagggtccct > 1:3282556‑M1/1‑61 (MQ=255) agcgagTTCCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTc < 1:934497/48‑1 (MQ=255) agcgagTTCCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTCAAGTTGTCATAAagggtccct > 1:2164203‑M1/1‑60 (MQ=255) agcgagTTCCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTCAAGTTGTCATAAagggtccct > 1:4184872‑M1/1‑60 (MQ=255) gcgagTTCCGTGCCGGTTGTGAAGAAAAAGTGAATGATGt > 1:2965246/1‑40 (MQ=255) agTTCCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTCAAGTTGTCATAAagggtccctaagtct < 1:2715279‑M1/71‑16 (MQ=255) gTTCCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTCaa < 1:4022539/45‑1 (MQ=255) tttCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTCAAGtt < 1:658117/47‑1 (MQ=255) tttCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTCAAGTTGTc < 1:2821632/50‑1 (MQ=255) | TTGGTTTTGACGATCAACTCTATTTCTCGCGAGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTTCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTCAAGTTGTCATAATTGGTAACGAATCAGACAA > W3110S.gb/71144‑71284 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |