Predicted mutation
evidence seq id position mutation freq annotation gene description
MC JC W3110S.gb 2,176,983 Δ1,199 bp 100% [gatA][gatA] [gatA], insH, [gatA]

Missing coverage evidence...
   seq id start end size ←reads reads→ gene description
* * ÷ W3110S.gb 2176983–2178180 2178181 2–1199 69 [0] [0] 69 [gatA]–[gatA] [gatA],insH,[gatA]

New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? W3110S.gb = 21769820 (0.000)54 (1.210) 30/88 NT 100% coding (71/321 nt) gatA galactitol‑specific enzyme IIA component of PTS
?W3110S.gb 2178182 = 0 (0.000)coding (202/315 nt) gatA galactitol‑specific enzyme IIA component of PTS

TCATCATCCGCTTGCTGAAAATGAACTTTATTTGTTGGCCTTAACAGATAAATGGCTGACGACTTAG‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  W3110S.gb/2176916‑2176982
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑cttagCATGAATCGCCTCACAATGCGGTATTGCAATAGCGTGCTGCTCAAGCATTATCCCGGTAGGGAATTCTG  >  W3110S.gb/2178182‑2178250
                                                                                                                                        
TCATCATCCGCTTGCTGAAAATGAAGTTTATTTGTTGGCCTTAACAGATAAATGGCTGACGACTTAGCAT                                                                    >  1:4235889/1‑70
TCATCATCCGCTTGCTGAAAATGAACTTTATTTGTTGGCCTTAACAGATAAATGGCTGACGACTTAGCAT                                                                    >  1:3623042/1‑70
     ATCCGCTTGCTGAAAATGAACTTTATTTGTTGGCCTTAACAGATAAATGGCTGACGACTTAGCATGAATCG                                                              >  1:1052052/1‑71
     ATCCGCTTGCTGAAAATGAACTTTATTTGTTGGCCTTAACAGATAAATGGCTGACGACTTAGCATGAATC                                                               <  1:2137926/70‑1
             GCTGAAAATGAACTTTATTTGTTGGCCTTAACAGATAAATGGCTGACGACTTAGCATGAATCGCCTCAC                                                        <  1:4049173/69‑1
             GCTGAAAATGAACTTTATTTGTTGGCCTTAACAGATAAATGGCTGACGACTTAGCATGAATCGCCTCAC                                                        <  1:613834/69‑1
               TGAAAATGAACTTTATTTGTTGGCCTTAACAGATAAATGGCTGACGACTTAGCATGAATCGCCTCAC                                                        <  1:4492364/67‑1
               TGAAAATGAACTTTATTTGTTGGCCTTAACAGATAAATGGCTGACGACTTAGCATGAATCGCCTCAC                                                        <  1:2702721/67‑1
                GAAAATGAACTTTATTTGTTGGCCTTAACAGATAAATGGCTGACGACTTAGCATGAATCGCCTCAC                                                        <  1:4558342/66‑1
                 AAAATGAACTTTATTTGTTGGCCTTAACAGATAAATGGCTGACGACTTAGCATGAATCGCCTCACAATGCt                                                  >  1:2369544/1‑70
                 AAAATGAACTTTATTTGTTGGCCTTAACAGATAAATGGCTGACGACTTAGCATGAATCGCCTCACAATGCG                                                  >  1:4664404/1‑71
                 AAAATGAACTTTATTTGTTGGCCTTAACAGATAAATGGCTGACGACTTAGCATGAATCGCCTCACAATGCG                                                  >  1:3612192/1‑71
                 AAAATGAACTTTATTTGTTGGCCTTAACAGATAAATGGCTGACGAC                                                                           >  1:1251784/1‑46
                  AAATGAACTTTATTTGTTGGCCTTAACAGATAAATGGCTGACGACTTAGCATGAATCGCCTCACAATGCGG                                                 <  1:4659261/71‑1
                  AAATGAACTTTATTTGTTGGCCTTAACAGATAAATGGCTGACGACTTAGCATGAATCGCCTCACAATGCGG                                                 <  1:4135103/71‑1
                  AAATGAACTTTATTTGTTGGCCTTAACAGATAAATGGCTGACGACTTAGCATGAATCGCCTCACAATGCGG                                                 <  1:4080329/71‑1
                   AATGAACTTTATTTGTTGGCCTTAACAGATAAATGGCTGACGACTTAGCATGAATCGCCTCACAATGCGG                                                 >  1:484420/1‑70
                   AATGAACTTTATTTGTTGGCCTTAACAGATAAATGGCTGACGACTTAGCATGAATCGCCTCACAATGCGG                                                 >  1:2846911/1‑70
                   AATGAACTTTATTTGTTGGCCTTAACAGATAAATGGCTGACGACTTAGCATGAATCGCCTCACAATGCGG                                                 >  1:3012893/1‑70
                   AATGAACTTTATTTGTTGGCCTTAACAGATAAATGGCTGACGACTTAGCATGAATCGCCTCACAATGCGG                                                 >  1:2406944/1‑70
                   AATGAACTTTATTTGTTGGCCTTAACAGATAAATGGCTGACGACTTAGCATGAATC                                                               >  1:4620393/1‑56
                   AATGAACTTTATTTGTTGGCCTTAACAGATAAATGGCTGACGACTTAGCATGA                                                                  <  1:1007456/53‑1
                    ATGAACTTTATTTGTTGGCCTTAACAGATAAATGGCTGACGACTT                                                                         <  1:284675/45‑1
                     TGAACTTTATTTGTTGGCCTTAACAGATAAATGGCTGACGACTTAGCATGAAT                                                                >  1:2261904/1‑53
                        ACTTTATTTGTTGGCCTTAACAGATAAATGGCTGACGACTTAGCATGAATCTCCTCACAATGCGGTATTGC                                           <  1:4540127/71‑1
                        ACTTTATTTGTTGGCCTTAACAGATAAATGGCTGACGACTTAGCATGAATCGCCTCACAATGCGGTATTGC                                           <  1:4684624/71‑1
                          TTTATTTGTTGGCCTTAACAGATAAATGGCTGACGACTTAGCATGAATCGCCTCACAATGCGGTATTGCAA                                         >  1:2424026/1‑71
                           TTATTTGTTGGCCTTAACAGATAAATGGCTGACGACTTAGCATGAATCGCCTCACAATGCGGTATTGCAA                                         >  1:4455334/1‑70
                           TTATTTGTTGGCCTTAACAGATAAATGGCTGACGACTTAGCATGAAT                                                                >  1:4745840/1‑47
                           TTATTTGTTGGCCTTAACAGATAAATGGCTGACGACTTAGCATGAAT                                                                >  1:3316419/1‑47
                            TATTTGTTGGCCTTAACAGATAAATGGCTGACGACTTAGCATGAATCGCCTCACAATGCGGTATTGCAA                                         <  1:1294602/69‑1
                            TATTTGTTGGCCTTAACAGATAAATGGCTGACGACTTAGCATGAATCGC                                                             >  1:4206538/1‑49
                             ATTTGTTGGCCTTAACAGATAAATGGCTGACGACTTAGCATGAAT                                                                >  1:4565060/1‑45
                             ATTTGTTGGCCTTAACAGATAAATGGCTGACGACTTAGCATGAAT                                                                >  1:2347129/1‑45
                             ATTTGTTGGCCTTAACAGATAAATGGCTGACGACTTAGCATGAAT                                                                >  1:4559832/1‑45
                              TTTGTTGGCCTTAACAGATAAATGGCTGACGACTTAGCATGAATCGCCTCACAATGCGGTATTGCAATAGC                                     >  1:3264644/1‑71
                              TTTGTTGGCCTTAACAGATAAATGGCTGACGACTTAGCATGAAT                                                                >  1:4603349/1‑44
                                TGTTGGCCTTAACAGATAAATGGCTGACGACTTAGCATGAATCGCCTCACAATGCGGTATTGCAA                                         >  1:4777328/1‑65
                                 GTTGGCCTTAACAGATAAATGGCTGACGACTTAGCATGAATCGCCTCACAATGC                                                   <  1:2587398/54‑1
                                  TTGGCCTTAACAGATAAATGGCTGACGACTTAGCATGAATCGCCTCACAATGCGGTATTGCAATAGCGTGC                                 <  1:2868738/71‑1
                                      CCTTAACAGATAAATGGCTGACGACTTAGCATGAATCGCCTCACAATGCGGTATTGCA                                          <  1:1591969/58‑1
                                      CCTTAACAGATAAATGGCTGACGACTTAGCATGAATCGCCTCACAATGCGG                                                 <  1:1834011/51‑1
                                          AACAGATAAATGGCTGACGACTTAGCATGAATCGCCTCACAATGCGGTATTGCAATAGCGTGCTGCTCAAG                         >  1:2787731/1‑71
                                          AACAGATAAATGGCTGACGACTTAGCATGAATCGCCTCACAATGCGGTATTGCAATAGCGTGCTGCTCA                           <  1:2508511/69‑1
                                          AACAGATAAATGGCTGACGACTTAGCATGAATCGCCTCACAATGCGGTATTGCAATAGCGTGCTGCTC                            >  1:862728/1‑68
                                           ACAGATAAATGGCTGACGACTTAGCATGAATCGCCTCACAATGCGGTATTGCAATAGCGTGCTGCTCAAG                         >  1:4662740/1‑70
                                            CAGATAAATGGCTGACGACTTAGCATGAATCGCCTCACAATGCGGTATTGCAATAGCGTGCTGCTCAAGCA                       <  1:1269983/71‑1
                                             AGATAAATGGCTGACGACTTAGCATGAATCGCCTCACAATGCGGTATTGCAATAGCGTGCTGCTCAAGCAT                      <  1:389101/71‑1
                                              GATAAATGGCTGACGACTTAGCATGAATCGCCTCACAATGCGGTATTGCAATAGCGTGCTGCTCAAGCATT                     <  1:4215444/71‑1
                                               ATAAATGGCTGACGACTTAGCATGAATCGCCTCACAATGCGGTATTGCAATAGCGTGCT                                <  1:2660026/59‑1
                                               ATAAATGGCTGACGACTTAGCATGAATCGCCTCACAATGCGGTATTGCAATAGCGTGC                                 <  1:1060096/58‑1
                                               ATAAATGGCTGACGACTTAGCATGAATCGCCTCACAATGCGGTATTG                                            <  1:4332827/47‑1
                                               ATAAATGGCTGACGACTTAGCATGAATCGCCTCACAATCCGGTATTGCAATAGCGTGCTGCTCAAGCAT                      <  1:542338/69‑1
                                               ATAAATGGCTGACGACTTAGCATGAATCGCCTCAC                                                        <  1:4628086/35‑1
                                                TAAATGGCTGACGACTTAGCATGAATCGCCTCACAATGCGGTATTGCAATAGCGTGCTGCTCAAGCATTAT                   <  1:4526623/71‑1
                                                TAAATGGCTGACGACTTAGCATGAATCGCCTCACAATGCGGTATTGCAATAGCGTGCTGCTCAAGC                        >  1:1973123/1‑66
                                                 AAATGGCAGACGACTTAGCATGAATCGCCTCACAATGCGGTATTGCAATAGCGTGC                                 >  1:4083758/1‑56
                                                  AATGGCTGACGACTTAGCATGAATCGCCTCACAATGCGGTATTGCAATAGCGTGCTGCTCAAGCATTATCC                 >  1:934440/1‑71
                                                        TGACGACTTAGCATGAATCGCCTCACAATGCGGTATTGCA                                          <  1:906836/40‑1
                                                          ACGACTTAGCATGAATCGCCTCACAATGCGGTATTGCAATAGCGTGCTGCTCAAGCATTATCCCGGTAGGG         <  1:3847264/71‑1
                                                          ACGACTTAGCATGAATCGCCTCACAATGCGGTATTGCAATAGCGTGCTGCTCAAGCATTATCCCGGTAGG          >  1:609614/1‑70
                                                             ACTTAGCATGAATCGCCTCACAATGCGGTATTGCAATAGCGTGCTGCTCAAGC                        <  1:812011/53‑1
                                                              CTTAGCATGAATCGCCTCACAATGCGGTATTGCAATAGCGTGCTGCTCAAGCATTATCCCGGTAGGGAA       <  1:2681877/69‑1
                                                              CTTAGCATGAATCGCCTCACAATGCGGTATTGCAATAGCGTGCTGCTCAAGCATTATCCCGCTAGGGAA       <  1:3214038/69‑1
                                                               TTAGCATGAATCGCCTCACAATGCGGTATTGCAATAGCGTGCTGCTCAAGCATTATCCC                <  1:1219086/59‑1
                                                               TTAGCATGAATCGCCTCACAATGCGGTATTGCAATAGCGTGCTGCTCAAGCAT                      >  1:2978179/1‑53
                                                               TTAGCATGAATCGCCTCACAATGCGGTATTGCAATAGCGTGCTGCTCAAGCAT                      <  1:689258/53‑1
                                                                TAGCATGAATCGCCTCACAATGCGGTATTGCAATAGC                                     >  1:3467537/1‑37
                                                                 AGCATGAATCGCCTCACAATGCGGTATTGCAATAGCGTGCTGCTCAAGCATTATCCCGGTAGGGAATTCTG  >  1:4017095/1‑71
                                                                 AGCATGAATCGCCTCACAATGCGGTATTGCAATAGCGTGCTGCTCAAGCATTATCCCGGTAGGGAATTCTG  >  1:2453918/1‑71
                                                                 AGCATGAATCGCCTCACAATGCGGTATTGCAATAGCGTGCTGCTCAAGCATTATCCCGGTAGGGAATTCTG  >  1:1618759/1‑71
                                                                                                                                        
TCATCATCCGCTTGCTGAAAATGAACTTTATTTGTTGGCCTTAACAGATAAATGGCTGACGACTTAG‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  W3110S.gb/2176916‑2176982
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑cttagCATGAATCGCCTCACAATGCGGTATTGCAATAGCGTGCTGCTCAAGCATTATCCCGGTAGGGAATTCTG  >  W3110S.gb/2178182‑2178250

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.