| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 907,713 | G→A | 33.3% | T331I (ACC→ATC) | ybjT ← | conserved hypothetical protein with NAD(P)‑binding Rossmann‑fold domain |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 907,713 | 0 | G | A | 33.3% | 34.9 / 24.7 | 30 | T331I (ACC→ATC) | ybjT | conserved hypothetical protein with NAD(P)‑binding Rossmann‑fold domain |
| Reads supporting (aligned to +/- strand): ref base G (11/9); new base A (5/5); total (16/14) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.67e-01 | |||||||||||
ACGCTCTTTACCGCCGATTTGGTTCACTACCTGCCATAAAGCAGCAAGGCTGGCGGACGTTTTAACGGTAAACCCCGCCTGTTTGGCAAAATAACCGTACTCCGGTCGCCAGCGGGCAAAGGCCTGAGCGTCGTA > W3110S.gb/907646‑907780 | aCGCTCTTTACCGCCGATTTGGTTCACTACCTGCCATAAAGCAGCAAGGCTGGCGGACGTTTTAACGGTa < 1:4267886/70‑1 (MQ=255) ctTTACCGCCGATTTGGTTCACTACCTGCCATAAAGCAGCAAGGCTGGCGGACGTTTTAACGGTAAAcccc > 1:4762872/1‑71 (MQ=255) ctTTACCGCCGATTTGGTTCACTACCTGCCATAAAGCAGCAAGGCTGGCGGACGTTTTAACGGTAAAcccc > 1:3587864/1‑71 (MQ=255) tACCGCCGATTTGGTTCACTACCTGCCATAAAGCAGCAAGGCTGGCGGACGTTTTAACGGTAAACCCCg < 1:2676225/69‑1 (MQ=255) tACCGCCGATTTGGTTCACTACCTGCCATAAAGCAGCAAGGCTGGCGGACGTTTTAACGGTAAACCCCGcc > 1:2765085/1‑71 (MQ=255) tACCGCCGATTTGGTTCACTACCTGCCATAAAGCAGCAAGGCTGGCGGACGTTTTAACGATaaa > 1:2188585/1‑64 (MQ=255) gTTCACTACCTGCCATAAAGCAGCAAGGCTGGCGGACGTTTTAACGATAAACCCCGCCTGTTTGGCAAAAt < 1:4500957/71‑1 (MQ=255) cACTACCTGCCATAAAGCAGCAAGGCTGGCGGACGTTTTAACGGTAAAcccc < 1:2500913/52‑1 (MQ=255) cACTACCTGCCATAAAGCAGCAAGGCTGGCGGACGTTTTAACGGTAAACCCCGCCTGTTTGGCAAAATAAc > 1:4702469/1‑71 (MQ=255) cACTACCTGCCATAAAGCAGCAAGGCTGGCGGACGTTTTAACGATAAACCCCGCCTGTTTGGCAAAATaa < 1:1384081/70‑1 (MQ=255) cTACCTGCCATAAAGCAGCAAGGCTGGCGGACGTTTTAACGGTAAACCCCGc > 1:984699/1‑52 (MQ=255) cTACCTGCCATAAAGCAGCAAGGCTGGCGGACGTTTTAACGGTAAACCCCGCCTGTTTGGCAAAATAACCg < 1:681149/71‑1 (MQ=255) aCCTGCCATAAAGCAGCAAGGCTGGCGGACGTTTTAACGATAAACCCCGCCTGTTTGGCAAAATAACCGt > 1:4195070/1‑70 (MQ=255) cTGCCATAAAGCAGCAAGGCTGGCGGACGTTTTAACGGTAAACCCCGCCTGTTTGGCAAAATAACCGTACt < 1:1215352/71‑1 (MQ=255) cTGCCATAAAGCAGCAAGGCTGGCGGACGTTTTAACGGTAAACCCCGCCTGTTTGGCAAAATAACCGTACt < 1:4248444/71‑1 (MQ=255) gCCATAAAGCAGCAAGGCTGGCGGACGTTTTAACGGTAAACCCCGCCTGTTTGGCAAAATAACCGTACTcc > 1:1767191/1‑71 (MQ=255) ccATAAAGCAGCAAGGCTGGCGGACGTTTTAACGATAAACCCCGCCTGTTTGGCAAAATAACCGTACTcc < 1:850419/70‑1 (MQ=255) ccATAAAGCAGCAAGGCTAGCGGACGTTTTAACGATAAACCCCGCCTGTTTGGCAAAATAACCGTACTcc < 1:3585995/70‑1 (MQ=255) cATAAAGCAGCAAGGCTGGCGGACGTTTTAACGGTAAACCCCGCCTGTTTGGCAAAATAACCGTACTCCgg > 1:1886989/1‑71 (MQ=255) cATAAAGCAGCAAGGCTGGCGGACGTTTTAACGGTAAACCCCGCCTGTTTGGCAAAATAACCGTACTCCgg < 1:115444/71‑1 (MQ=255) aTAAAGCAGCAAGGCTGGCGGACGTTTTAACGGTAAACCCCGCCTGTTTGGCAAAATAACCGTACTCCGGt > 1:3087919/1‑71 (MQ=255) aaGCAGCAAGGCTGGCGGACGTTTTAACGGTAAACCCCGCCTGTTTGGCAAAATAACCGTACTCCGGTCGc > 1:1708845/1‑71 (MQ=255) caAGGCTGGCGGACGTTTTAACGGTAAACCCCGGCTGTTTGGCAAAATAAc > 1:2572915/1‑51 (MQ=255) aaGGCTGGCGGACGTTTTAACGGTAAACCCCGCCTGTTTGGCAAAATAACCGTAc > 1:745855/1‑55 (MQ=255) aaGGCTGGCGGACGTTTTAACGATAAACCCCGCCTGTTTGGCAAAATAACCGTACTCCGGTCGCCa > 1:1438320/1‑66 (MQ=255) ggCTGGCGGACGTTTTAACGATAAACCCCGCCTGTTTGGCAAAATAACCGTACTCCGGTCGCCAGCGGGCa < 1:3088618/71‑1 (MQ=255) gCTGGCGGACGTTTTAACGGTAAACCCCGCCTGTTTGGCAAAATAACCGTACTCCGGTCGCCAGCGGGCaa < 1:1920712/71‑1 (MQ=255) gCTGGCGGACGTTTTAACGATAAACCCCGCCTGTTTGGCAAAATAACCGTACTCCGGTCGCCAGCGGGCa > 1:2641175/1‑70 (MQ=255) ttAACGATAAACCCCGCCTGTTTGGCAAAATAACCGTACTCCGGTCGCCa > 1:1595526/1‑50 (MQ=255) aCGGTAAACCCCGCCTGTTTGGCAAAATAACCGTACTCCGGTCGCCAGCGGGCAAAGGCCTGAGCGTCGTa < 1:3900620/71‑1 (MQ=255) | ACGCTCTTTACCGCCGATTTGGTTCACTACCTGCCATAAAGCAGCAAGGCTGGCGGACGTTTTAACGGTAAACCCCGCCTGTTTGGCAAAATAACCGTACTCCGGTCGCCAGCGGGCAAAGGCCTGAGCGTCGTA > W3110S.gb/907646‑907780 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |