| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 2,286,501 | C→A | 30.3% | A261S (GCG→TCG) | yejK ← | nucleotide associated protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,286,501 | 0 | C | A | 30.3% | 119.0 / 33.1 | 56 | A261S (GCG→TCG) | yejK | nucleotide associated protein |
| Reads supporting (aligned to +/- strand): ref base C (15/24); new base A (7/10); total (22/34) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.80e-01 | |||||||||||
AACTGACGCAGTGTGCTACGGTCTGCCGGGAAGCTCTCTTCCAGTTCGTAGCCTTTTTCAGCCGCAAACTCCGTGAAGCTCACTTCGCTTACGCCTGCCAGCTCTTTCGACAGCGATTTCAGTTCGATCTCTTCG > W3110S.gb/2286437‑2286571 | aaCTGACGCAGTGTGCTACGGTCTGCCGGGAAGCTCTCTTCCAGTTCGTAGCCTTTTTCAGCCGCAAACTc > 1:3812180/1‑71 (MQ=255)aaCTGACGCAGTGTGCTACGGTCTGCCGGGAAGCTCTCTTCCAGTTCGTAGCCTTTTTCAGCCGAAAACt > 1:3330055/1‑70 (MQ=255) aGTGTGCTACGGTCTGCCGGGAAGCTCTCTTCCAGTTCGTAGCCTTTTTCAGCCGCAAACTCCGTGAAGCt < 1:1356989/71‑1 (MQ=255) tgtgCTACGGTCTGCCGGGAAGCTCTCTTCCAGTTCGTAGCCTTTTTCAGCCGCAAACTCCGTg < 1:3590264/64‑1 (MQ=255) tgtgCTACGGTCTGCCGGGAAGCTCTCTTCCAGTTCGTAGCCTTTTTCAGCCGCAAACTCCGTg < 1:3433589/64‑1 (MQ=255) tgtgCTACGGTCTGCCGGGAAGCTCTCTTCCAGTTCGTAGCCTTTTTCAGCCGCAAACTCCGTGAAGCt < 1:3278891/69‑1 (MQ=255) gtgCTACGGTCTGCCGGGAAGCTCTCTTCCAGTTCGTAGCCTTTTTCAGCCGCaaa > 1:3656612/1‑56 (MQ=255) gtgCTACGGTCTGCCGGGAAGCTCTCTTCCAGTTCGTAGCCTTTTTCAGCCGCaaa > 1:3030780/1‑56 (MQ=255) tgCTACGGTCTGCCGGGAAGCTCTCTTCCAGTTCGTAGCCTTTTTCAGCCGCAAACTCCGTGAAGCTCACt > 1:4361834/1‑71 (MQ=255) gCTACGGTCTGCCGGGAAGCTCTCTTCCAGTTCGTAGCCTTTTTCAGCCGCAAACTCCGTGAAGCTCACtt > 1:2970315/1‑71 (MQ=255) cTACGGTCTGCCGGGAAGCTCTCTTCCAGTTCGTAGCCTTTTTCAGCCGCAAACTCCGTGAAGCTCACTTc > 1:965214/1‑71 (MQ=255) cTACGGTCTGCCGGGAAGCTCTCTTCCAGTTCGTAGCCTTTTTCAGCCGAAAACTCCGTg < 1:4424543/60‑1 (MQ=255) cTACGGTCTGCCGGGAAGCTCTCTTCCAGTTCGTAGCCTTTTTCAGCCGAAAACTCCGTGAAGCTCACTTc < 1:3258304/71‑1 (MQ=255) cTACGGTCTGCCGGGAAGCTCTCTTCCAGTTCGTAGCCTTTTTCAGCCGAAAACTCCGTGAAGCTCACTTc < 1:297818/71‑1 (MQ=255) aCGGTCTGCCGGGAAGCTCTCTTCCAGTTCGTAGCCTTTTTCAGCCGAAAACTCCGTGaa > 1:2143440/1‑60 (MQ=255) cGGTCTGCCGGGAAGCTCTCTTCCAGTTCGTAGCCTTTTTCAGCCGCAAACTCCGTGAAGCTCACTTCGCt < 1:1489437/71‑1 (MQ=255) cGGTCTGCCGGGAAGCTCTCTTCCAGTTCGTAGCCTTTTTCAGCCGCAAACTCCGTGAAGCTCACTTCGCt < 1:2995670/71‑1 (MQ=255) cGGTCTGCCGGGAAGCTCTCTTCCAGTTCGTAGCCTTTTTCAGCCGCAAACTCCGTGAAGCTCACTTCGCt < 1:2335631/71‑1 (MQ=255) cGGTCTGCCGGGAAGCTCTCTTCCAGTTCGTAGCCTTTTTCAGCCGCAAACTCCGTGAAGCTCACTTCGCt < 1:748243/71‑1 (MQ=255) cGGTCTGCCGGGAAGCTCTCTTCCAGTTCGTAGCCTTTTTCAGCCGCAAACTCCGTGAAGCTCACTTCGCt < 1:2779678/71‑1 (MQ=255) ggTCTGCCGGGAGGCTCTCTTCCAGTTCGTAGCCTTTTTCAGCCGCAAACTCCGTGAAGCTCACTTCGCt > 1:4624324/1‑70 (MQ=255) ccGGGAAGCTCTCTTCCAGTTCGTAGCCTTTTTCAGCCGCAAACTCCGTGAAGCTCACTTCGCTTACGCCt > 1:2589619/1‑71 (MQ=255) ccGGGAAGCTCTCTTCCAGTTCGTAGCCTTTTTCAGCCGAAAACTCCGTGAAGCTCACTTCGCTTACGCCt > 1:849511/1‑71 (MQ=255) aaGCTCTCTTCCAGTTCGTAGCCTTTTTCAGCCGCAAACTCCGTGAAGCTCACTTCGCTTACGCCTGCCa > 1:3960335/1‑70 (MQ=255) aGCTCTCTTCCAGTTCGTAGCCTTTTTCAGCCGAAAACTCCGTGAAGCTCACTTCGCTTACGCCTGCCAGc < 1:3485996/71‑1 (MQ=255) gCTCTCTTCCAGTTCGTAGCCTTTTTCAGCCGCAAACTCCGTGAAGCTCACTTCGCTTACGCCTGCCAGct < 1:14126/71‑1 (MQ=255) ctctctTCCAGTTCGTAGCCTTTTTCAGCCGCAAACTCCGTGAAGCTCACTTCGCt < 1:880056/56‑1 (MQ=255) tctctTCCAGTTCGTAGCCTTTTTCAGCCGCAAACTCCGTGAAGCTCACTTCGCTTACGCCTGCCAGctct > 1:555330/1‑71 (MQ=255) ctctTCCAGTTCGTAGCCGTTTTCAGCCGCAAACTCCGTGAAGCTCACTTCGCTTACGCCTGCCAGCTCtt > 1:3300818/1‑71 (MQ=255) tctTCCAGTTCGTAGCCTTTTTCAGCCGCAAACTCCGTGAAGCTCACTTCGCt < 1:1191782/53‑1 (MQ=255) tctTCCAGTTCGTAGCCTTTTTCAGCCGCAAACTCCGTGAAGCTCACTTCGCt < 1:359489/53‑1 (MQ=255) tctTCCAGTTCGTAGCCTTTTTCAGCCGAAAACTCCg < 1:4290031/37‑1 (MQ=255) tctTCCAGTTCGTAGCCTTTTTCAGCCGAAAACTCCg < 1:1173658/37‑1 (MQ=255) ttCCAGTTCGTAGCCTTTTTCAGCCGCAAACTCCGTGAAGCTCACTTCGCTTa > 1:3084791/1‑53 (MQ=255) ttCCAGTTCGTAGCCTTTTTCAGCCGCAAACTCCGTGAAGCTCACTTCGCTTACGCCTGCCAGCTCtt < 1:394378/68‑1 (MQ=255) ttCCAGTTCGTAGCCTTTTTCAGCCGCAAACTCCGTGAAGCTCACTTCGCTTACGCCTGCCAGCTCtt < 1:2916065/68‑1 (MQ=255) ttCCAGTTCGTAGCCTTTTTCAGCCGAAAACTCCGTg > 1:1736825/1‑37 (MQ=255) ccAGTTCGTAGCCTTTTTCAGCCGCAAACTCCGTGAAg > 1:2284608/1‑38 (MQ=255) ccAGTTCGTAGCCTTTTTCAGCCGCAAACTCCGTGAAGCTCACTTCGCTTACGCCTGCCAGCTCTTTc < 1:1100217/68‑1 (MQ=255) ccAGTTCGTAGCCTTTTTCAGCCGCAAACTCCGTGAAGCTCACTTCGCTTACGCCTGCCAGCTCTTTc < 1:4708200/68‑1 (MQ=255) ccAGTTCGTAGCCTTTTTCAGCCGAAAACTCCGTGAAGCTCACTTCGCTTACGCCTGCCAGCTCTTTc < 1:1065850/68‑1 (MQ=255) ccAGTTCGTAGCCTTTTTCAGCCGAAAACTCCGTGAACCTCACTTCGCTTACGCCTGCCAGCTCTTTc < 1:1915761/68‑1 (MQ=255) acAGTTCGTAGCCTTTTTCAGCCGCAAACTCCGTGAAg > 1:1901619/2‑38 (MQ=255) cAGTTCGTAGCCTTTTTCAGCCGCAAACTCCGTTAAGCTCACTTCGCTTACGCCTGCCAGCTCTTTc < 1:2295713/67‑1 (MQ=255) ttCGTAGCCTTTTTCAGCCGCAAACTCCGTGAAGCTCACTTCGCTTAc < 1:2699728/48‑1 (MQ=255) cGTAGCCTTTTTCAGCCGCAAACTCCGTGAAGCTCACTTCGCTTACGTCTGCCAGCTCTTTCGACAGCGa < 1:4525430/70‑1 (MQ=255) tAGCCTTTTTCAGCCGAAAACTCCGTGAAGCTCACTTCGCTTACGCCTGCCAGCTCTTTCGACAGCGAttt > 1:1046678/1‑71 (MQ=255) tAGCCTTTTTCAGCCGAAAACTCCGTGAAGCTCACTTCGCTTACGCCTGCCAGCTCTTTCGACAGCGAttt < 1:1766537/71‑1 (MQ=255) gCCTTTTTCAGCCGAAAACTCCGTGAAGCTCACTTCGCTTACGCCTGCCAGCTCTTTCGACAGCGAttt > 1:3939804/1‑69 (MQ=255) gCCTTTTTCAGCCGAAAACTCCGTGAAGCTCACTTCGCTTACGCCTGCCAGCTCTTTCGACAGCGAttt > 1:4721423/1‑69 (MQ=255) ccTTTTTCAGCCGCAAACTCCGTGAAGCTCACTTCg > 1:494773/1‑36 (MQ=255) tttttCAGCCGCAAACTCCGTGAAGCTCACTTCGCTTACGCCTGCCAGCTCTTTCGACAGCGATTTc < 1:3037260/67‑1 (MQ=255) ttCAGCCGCAAACTCCGTGAAGCTCACTTCGCTTACGCCTGCCAGCTCTTTCGACAGCGATTTCAGTTCg < 1:2159487/70‑1 (MQ=255) ttCAGCCGCAAACTCCGTGAAGCTCACTTCGCTTACGCCTGCCAGCTCTTTCGACAGCGATTTCAGTTCg < 1:4563093/70‑1 (MQ=255) ttCAGCCGCAAACTCCGTGAAGCTCACTTCGCTTACGCCTGCCAGCTCTTTCGACAGCGATTTCAGTTCg < 1:129831/70‑1 (MQ=255) ccGAAAACTCCGTGAAGCTCACTTCGCTTACGCCTGCCAGCTCTTTCGACAGCGATTTCAGTTCGATctct < 1:3803286/71‑1 (MQ=255) cAAACTCCGTGAAGCTCACTTCGCTTACGCCTGCCAGCTCTTTc > 1:3968135/1‑44 (MQ=255) cAAACTCCGTGAAGCTCACTTCGCTTACGCCTGCCAGCTCTTTCGACAGCGATTTCAGTTCGATCTCTTCg > 1:1458620/1‑71 (MQ=255) | AACTGACGCAGTGTGCTACGGTCTGCCGGGAAGCTCTCTTCCAGTTCGTAGCCTTTTTCAGCCGCAAACTCCGTGAAGCTCACTTCGCTTACGCCTGCCAGCTCTTTCGACAGCGATTTCAGTTCGATCTCTTCG > W3110S.gb/2286437‑2286571 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |