| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 4,246,990 | C→T | 16.7% | R40H (CGT→CAT) | malG ← | maltose transporter subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 4,246,990 | 0 | C | T | 16.7% | 90.4 / 10.5 | 36 | R40H (CGT→CAT) | malG | maltose transporter subunit |
| Reads supporting (aligned to +/- strand): ref base C (18/12); new base T (3/3); total (21/15) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.77e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.57e-01 | |||||||||||
AGTTTCCAGTGATCCCAGGAGATTTGCTCCGGGATCAGGCTGCCGGTCGCAAAGTTTCCCTGACGCAGCGAGATAGCGACGACCATCAGCAGCGGGAACATAATCGCTGCGATAAAAAGTAGCAGTAGCA > W3110S.gb/4246927‑4247056 | aGTTTCCAGTGATCCCAGGAGATTTGCTCCGGGATCAGGCTGCCGGTCGCAAAGTATCCCTGACGCAGCga < 1:4024903/71‑1 (MQ=255) tttCCAGTGATCCCAGGAGATTTGCTCCGGGATCAGGCTGCCGGTCGCAAAGTTTCCCTGACGCAGCgaga < 1:4178738/71‑1 (MQ=255) tCCAGTGATCCCAGGAGATTTGCTCCGGGATCAGGCTGCCGGTCGCAAAGTTTCCCTGATGCAGCgaga > 1:644086/1‑69 (MQ=255) tCCAGTGATCCCAGGAGATTTGCTCCGGGATCAGGCTGCCGGTCGCAAAGTTTCCCTGACGCAGCGAGAt < 1:2088378/70‑1 (MQ=255) gATCCCAGGAGATTTGCTCCGGGATCAGGCTGCCGGTCGCAAAGTTTCCCTGACGCAGCGAGATAGcgac > 1:1234767/1‑70 (MQ=255) aTCCCATGAGATTTGCTCCGGGATCAGGCTGCCGGTCGCAAAGTTTCCCTGACGCAGCGAGATAGcgacga > 1:1048170/1‑71 (MQ=255) ggAGATTTGCTCCGGGATCAGGCTGCCGGTCGCAAAGTTTCCCTGACGCAGCGAGATAGCGACGACCAt > 1:3090201/1‑69 (MQ=255) ttGCTCCGGGATCAGGCTGCCGGTCGCAAAGTTTCCCTGACGCAGCGAGATAGCGACGACCATCAGCAGCg > 1:1507000/1‑71 (MQ=255) tGCTCCGGGATCAGGCTGCCGGTCGCAAAGTTTCCCTGACGCAGCGAGATAGCGACGACCATCAGCAGCgg > 1:1091960/1‑71 (MQ=255) tGCTCCGGGATCAGGCTGCCGGTCGCAAAGTTTCCCTGACGCAGCGAGATAGCGACGACCATCAGCAGCgg > 1:531950/1‑71 (MQ=255) gCTCCGGGATCAGGCTGCCGGTCGCAAAGTTTCCCTGACGCAGCGAGATAGCGACGACCATCAGCAGCg > 1:4678859/1‑69 (MQ=255) gCTCCGGGATCAGGCTGCCGGTCGCAAAGTTTCCCTGACGCAGCGAGATAGCGACGACCATCAGCAGCg > 1:21589/1‑69 (MQ=255) cGGGATCAGGCTGCCGGTCGCAAAGTTTCCCTGACGCAGCGAGATAGCGACGACCATc < 1:2314917/58‑1 (MQ=255) gggATCAGGCTGCCGGTCGCAAAGTTTCCCTGATGCAGCGAGATAGCGACGACCATCAGCAGCGGGAACAt < 1:1367596/71‑1 (MQ=255) aGGCTGCCGGTCGCAAAGTTTCCCTGACGCAGCGAGATAGCGACGACCATCAGCAGCGGGAACATAATCGc > 1:1422448/1‑71 (MQ=255) ccGGTCGCAAAGTTTCCCTGACGCAGCGAGATAGCGACGACCATCAGCAGCGGGAACATAATCGCTGCGa > 1:373395/1‑70 (MQ=255) ccGGTCGCAAAGTTTCCCTGACGCAGCGAGATAGCGACGACCATCAGCAGCGGGAACATAATCGCTGCGa > 1:2036188/1‑70 (MQ=255) ggTCGCAAAGTTTCCCTGACGCAGCGAGATAGCGACGACCATCAGCAGCGGGAACATAATCGCTGCGATa > 1:4644129/1‑70 (MQ=255) ggTCGCAAAGTTTCCCTGACGCAGCGAGATAGCGACGACCATCAGCAGCGGGAACATAATCGCTGCGATa > 1:4312835/1‑70 (MQ=255) gTCGCAAAGTTTCCCTGATGCAGCGAGATAGCGACGACCATCAGCAGCGGGAACATAATCGCTGCGATaaa < 1:1894338/71‑1 (MQ=255) tCGCAAAGTTTCCCTGACGCAGCGAGATAGCgacgac < 1:2448880/37‑1 (MQ=255) tCGCAAAGTTTCCCTGACGCAGCGAGATAGCgacgac < 1:1501470/37‑1 (MQ=255) gCAAAGTTTCCCTGACGCAGCGAGATAGCGACGACCATCAGCAGCGGGAACATAATCGCTGCGATaaa > 1:3150459/1‑68 (MQ=255) cAAAGTTTCCCTGACGCAGCGAGATAGCGACGACCATCAGCAGCGGGAACATAATCGCTGCGATAAAAag > 1:2061951/1‑70 (MQ=255) aaGTTTCCCTGATGCAGCGAGATAGCGACGACCATTAGCAGCGGGAACATAATCGCTGCGAGAAAAagtag > 1:91318/1‑71 (MQ=255) aaGTTTCCCTGACGCAGCGAGATAGCGACGACCATCAGCAGCGGGAACATAATCGCTGCGATAAAAagtag < 1:1853432/71‑1 (MQ=255) aGTTTCCCTGACGCAGCGAGATAGCGACGACCATCAGCAGCGGGAACATAATCGCTGCGATAAAAagtag > 1:1851730/1‑70 (MQ=255) tttCCCTGACGCAGCGAGATAGCGACGACCATCAGCAGCGGGAACATAATCGCTGCGATAAAAagtag < 1:2799199/68‑1 (MQ=255) tCCCTGACGCAGGGAGATAGCGACGACCATCAGCAGCGGGAACATAATCGCTGCGATAAAAagtagca > 1:4403414/1‑68 (MQ=255) tCCCTGACGCAGCGAGATAGCGACGACCATCAGCAGCGGGAACATAATCGCTGCGATAAAAagtagca > 1:3588939/1‑68 (MQ=255) ccTGATGCAGCGAGATAGCGACGACCATCAGCAGCGGGAACATAATCGCTGCGATAAAAagtagcagtag < 1:802072/70‑1 (MQ=255) cTGATGCAGCGAGATAGCGACGACCATCAGCAGCGGGAACATAATCGCTGCGATAAAAagtagcagtag > 1:1208180/1‑69 (MQ=255) cTGACGCAGCGAGATAGCGACGACCATCAGCAGCGGGAACATAATCGCTGCGAt < 1:4751476/54‑1 (MQ=255) cTGACGCAGCGAGATAGCGACGACCATCAGCAGCGGGAACATAATCGCTGCGATAAAAAgtagcagtagca < 1:338187/71‑1 (MQ=255) aCGCAGCGAGATAGCGACGACCATCAGCAGCGGGAACATAATCGCTGCGATAAAAag < 1:3056667/57‑1 (MQ=255) aCGCAGCGAGATAGCGACGACCATCAGCAGCGGGAACATAATCGCTGCGATAAAAag < 1:1111706/57‑1 (MQ=255) | AGTTTCCAGTGATCCCAGGAGATTTGCTCCGGGATCAGGCTGCCGGTCGCAAAGTTTCCCTGACGCAGCGAGATAGCGACGACCATCAGCAGCGGGAACATAATCGCTGCGATAAAAAGTAGCAGTAGCA > W3110S.gb/4246927‑4247056 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |