| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 4,416,062 | A→G | 33.3% | N27D (AAT→GAT) | yjfK → | conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 4,416,062 | 0 | A | G | 33.3% | 72.2 / 57.7 | 63 | N27D (AAT→GAT) | yjfK | conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base A (24/18); new base G (12/9); total (36/27) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TTTTTCCAGCGTCTGTTTGGCAAGGATAATAAGCCAGCTATCGCTCGTGGTCCGCTGGGACTTCATCTCAATAGTGGCTTTACGCTCGACACGTTAGCGTTTCGTTTGCTGGAAGATGAATTGCTGATTGCGCTGCCGG > W3110S.gb/4415993‑4416131 | tttttCCAGCGTCTGTTTGGCAAGGATAATAAGCCAGCTATCGCTCGTGGTCCGCTGGGACTTCATCTCaa > 1:3139041/1‑71 (MQ=255) tttCCAGCGTCTGTTTGGCAAGGATAATAAGCCAGCTATCGCTCGTGGTCCGCTGGGACTTCATCTCAAt < 1:2213280/70‑1 (MQ=255) aGCGTCTGTTTGGCAAGGATAATAAGCCAGCTATCGCTCGTGGTCCGCTGGGACTTCATCTCAATAGTGGc > 1:3658598/1‑71 (MQ=255) tGTTTGGCAAGGATAATAAGCCAGCTATCGCTCGTGGTCCGCTGGGACTTCATCTCGATAGTGGCTTTACg > 1:1106385/1‑71 (MQ=255) ttGGCAAGGATAATAAGCCAGCTATCGCTCGTGGTCCGCTGGGACTTCATCTCaa < 1:2876175/55‑1 (MQ=255) ttGGCAAGGATAATAAGCCAGCTATCGCTCGTGGTCCGCTGGGACTTCATCTCGATa > 1:4611170/1‑57 (MQ=255) ttGGCAAGGATAATAAGCCAGCTATCGCTCGTGGTCCGCTGGGACTTCATCTCGATAGTGGCTTTACGCTc > 1:532448/1‑71 (MQ=255) tGGCAAGGATAATAAGCCAGCTATCGCTCGTGGTCCGCTGGGACTTCATCTCAATAGTGGCTTTACGCTc < 1:3532129/70‑1 (MQ=255) tGGCAAGGATAATAAGCCAGCTATCGCTCGTGGTCCGCTGGGACTTCATCTCAATAGTGGCTTTACGCTc < 1:385210/70‑1 (MQ=255) tGGCAAGGATAATAAGCCAGCTATCGCTCGTGGTCCGCT‑‑GACTTCATCTCAATAGTGGCTTTACGCTc < 1:2072243/68‑1 (MQ=255) ggCAAGGATAATAAGCCAGCTAACGCTCGTGGTCCGCTGGGACTTCATCTCAATa > 1:1755948/1‑55 (MQ=255) gATAATAAGCCAGCTATCGCTCGTGGTCCGCTGGGACTTCATCTCAATAGTGGCTTTACGCTCGACACGt > 1:1308165/1‑70 (MQ=255) ataaGCCAGCTATCGCTCGTGGTCCGCTGGGACTTCATCTCAATAGTGGCTTTACGCTCGACACGTTAGcg > 1:4337776/1‑71 (MQ=255) aGCCACCTATCGCTCGTGGTCCGCTGCGACTTCATCTCGAt > 1:3008215/1‑41 (MQ=255) ccAGCTATCGCTCGTGGTCCGCTGGGACTTCATCTCGATAGTGGCTTTACGCTCGa < 1:4490239/56‑1 (MQ=255) aGCTATCGCTCGTGGTCCGCTGGGACTTCATCTCAATAGTGGCTTTACGCTCGACACGTTAGcgtttcgtt < 1:3603696/71‑1 (MQ=255) aGCTATCGCTCGTGGTCCGCTGGGACTTCATCTCAATAGTGGCTTTACGCTCGACACGTTAGcgtttcgtt > 1:465649/1‑71 (MQ=255) aGCTATCGCTCGTGGTCCGCTGGGACTTCATCTCAATAGTGGCTTTACGCTCGACACGTTAGcgtttcgtt < 1:787052/71‑1 (MQ=255) tATCGCTCGTGGTCCGCTGGGACTTCATCTCAATAGTGGCTTTACGCTCGACACGTTAGCGTTTCGTTTga > 1:4566419/1‑70 (MQ=255) tATCGCTCGTGGTCCGCTGGGACTTCATCTCAATAGTGGCTTTACGCTCGACACGTTAGCGTTTCGTTTGc > 1:2907536/1‑71 (MQ=255) tCGCTCGTGGTCCGCTGGGACTTCATCTCGATAGTGGCTTTACGCTCGACACGTTAGCGTTTCGTTTGCTg > 1:1860799/1‑71 (MQ=255) tCGCTCGTGGTCCGCTGGGACTTCATCTCGATAGTGGCTTTACGCTCGACACGTTAGCGTTTCGTTTGCTg < 1:3399339/71‑1 (MQ=255) tCGCTCGTGGTCCGCTGGGACTTCATCTCAATAGTGGCtt < 1:364490/40‑1 (MQ=255) tCGCTCGTGGTCCGCTGGGACTTCATCTCAATAGTGGCtt < 1:276066/40‑1 (MQ=255) tCGCTCGTGGTCCGCTGGGACTTCATCTCAATAGTGGCTTTACGCTCGACACGTTAGcgtttcgtt < 1:4444409/66‑1 (MQ=255) tCGCTCGTGGTCCGCTGGGACTTCATCTCAATAGTGGCTTTACGCTCGACACGTTAGCGTTTCGTTTGc < 1:522217/69‑1 (MQ=255) cGCTCGTGGTCCGCTGGGACTTCATCTCGATAGTGGCTTTACGCTCGACACGTTAGCGTTTCGTTTg < 1:3014097/67‑1 (MQ=255) cGCTCGTGGTCCGCTGGGACTTAATCTCAATAGTGGCt > 1:305616/1‑38 (MQ=255) gCTCGTGGTCCGCTGGGACTTCATCTCGATAGTGGCTTTa > 1:2573220/1‑40 (MQ=255) gCTCGTGGTCCGCTGGGACTTCATCTCGATAGTGGCTTTACGCTCGac > 1:4719883/1‑48 (MQ=255) gCTCGTGGTCCGCTGGGACTTCATCTCGATAGTGGCTTTACGCTCGac > 1:1367960/1‑48 (MQ=255) gCTCGTGGTCCGCTGGGACTTCATCTCGATAGTGGCTTTACGCTCGACACGTTAGCGTTTCGTTTGCTgg > 1:816763/1‑70 (MQ=255) cTCGTGGTCCGCTGGGACTTCATCTCGATAGTGGCTTTACGCTCGACACGTTAGCGTTTCGTTTGCt > 1:154607/1‑67 (MQ=255) tCGTGGTCCGCTGGGACTTCATCTCAATAGTGGCTTTACGCTCGACACGTTAGCGTTTCGTTTGCTgg > 1:519214/1‑68 (MQ=255) tCGTGGTCCGCTGGGACTTCATCTCAATAGTGGCTTTACGCTCGACACGTTAGCGTTTCGTTTGCTgg > 1:513465/1‑68 (MQ=255) tGGTCCGCTGGGACTTCATCTCAATAGTGGCTTTACGCTCGACACGTTAGCGTTTCGTTTGCTGGAAGATg < 1:4300747/71‑1 (MQ=255) tGGTCCGCTGGGACTTCATCTCAATAGTGGCTTTACGCTCGACACGTTAGCGTTTCGTTTGCTGGAAGATg < 1:4453933/71‑1 (MQ=255) tGGTCCGCTGGGACTTCATCTCAATAGTGGCTTTACGCTCGACACGTTAGCGTTTCGTTTGCTGGAAGATg < 1:1108198/71‑1 (MQ=255) ggTCCGCTGGGACTTCATCTCAATAGTGGCTTTACGCTCGACACGTTAGCGTTTCGTTTGCTGGAAGATg > 1:998395/1‑70 (MQ=255) ccGCTGGGACTTCATCTCAATAGTGGCTTTACGCTCGACACGTTAGCGTTTCGTTTGc > 1:4063250/1‑58 (MQ=255) cGCTGGGACTTCATCTCGATAGTGGCTTTACGCTCGACACGTTAGCGTTTCGTTTGCTGGAAGATGAATTg > 1:3137239/1‑71 (MQ=255) cGCTGGGACTTCATCTCAATAGTGGCTTTACGCTCGACACGTTAGcgtttcg < 1:2369201/52‑1 (MQ=255) gCTGGGACTTCATCTCAATAGTGGCTTTACGCTCGACACGTTAGCGTTTCGTTTGCTGGAAGTTGAATTGc < 1:4242138/71‑1 (MQ=255) cTGGGACTTCATCTCGATAGTGGCTTTACGCTCGACACGTTAGCGTTTCGTTTGCTGGAAGATGAATTGCt < 1:1333747/71‑1 (MQ=255) gggACTTCATCTCAATAGTGGCTTTACGCTCGACACGTTAGCGTTTCGTTTGCTGGAAGATGAATTGCt > 1:2592679/1‑69 (MQ=255) gggACTTCATCTCAATAGTGGCTTTACGCTCGACACGTTAGCGTTTCGTTTGCTGGAAGATGAATTGCTGa > 1:4656750/1‑71 (MQ=255) aCTTCATCTCAATAGTGGCTTTACGCTCGACACGTTAGCGTTTCGTTTGCTGGAAGATGaa > 1:3116860/1‑61 (MQ=255) aCTTCATCTCAATAGTGGCTTTACGCTCGACACGTTAGCGTTTCGTTTGCTGGAAGATGaa > 1:659282/1‑61 (MQ=255) aCTTCATCTCAATAGTGGCTTTACGCTCGACACGTTAGCGTTTCGTTTGCTGGAAGATGaa > 1:1565081/1‑61 (MQ=255) aCTTCATCTCAATAGTGGCTTTACGCTCGACACGTTAGCGTTTCGTTTGCTGGAAGATGAATTGCTGATTg < 1:4727082/71‑1 (MQ=255) cTTCATCTCAATAGTGGCTTTACGCTCGACACGTTAGCGTTTCGTTTGCTGGAAGATGAATTGCTGATTgc < 1:1519976/71‑1 (MQ=255) ttCATGTCGATAGTGGCTTTACGCTCGACACGTTAGCGTTTCGTTTGCTGGAAGATGAATTg < 1:1897592/62‑1 (MQ=255) ttCATCTCGATAGTGGCTTTACGCTCGACACGTTAGCGTTTCGTTTGCTGGAAGATg < 1:2032833/57‑1 (MQ=255) ttCATCTCGATAGTGGCTTTACGCTCGACACGTTAGCGTTTCGTTTGCTGGAAGATGAATTg < 1:4799426/62‑1 (MQ=255) ttCATCTCGATAGTGGCTTTACGCTCGACACGTTAGCGTTTCGTTTGCTGGAAGATGAATTg < 1:2068531/62‑1 (MQ=255) ttCATCTCAATAGTGGCTTTACGCTCGACACGTTAGcgtttcgtt > 1:4009317/1‑45 (MQ=255) ttCATCTCAATAGTGGCTTTACGCTCGACACGTTAGcgtttcgtt > 1:3561575/1‑45 (MQ=255) ttCATCTCAATAGTGGCTTTACGCTCGACACGTTAGCGTTTCGTTTGCTGGAATATg < 1:2810246/57‑1 (MQ=255) tCATCTCGATAGTGGCTTTACGCTCGACACGTTAGCGTTTCGTTTGCTGGAAGATGAATTGCTGATTgcgc > 1:2747213/1‑71 (MQ=255) aTCTCAATAGTGGCTTTACGCTCGACACGTTAGCGTTTCGTTTGCTGGAAGATGAATTGCTGATTGCGCTg > 1:1336530/1‑71 (MQ=255) tctcGATAGTGGCTTTACGCTCGACACGTTAGCGTTTCGTTTGCTGGAAGATGAATTGCTGATTgc < 1:3101992/66‑1 (MQ=255) tcAATAGTGGCTTTACGCTCGACACGTTAGcgtttcgt > 1:4025090/1‑38 (MQ=255) tcAATAGTGGCTTTACGCTCGACACGTTAGCGTTTCGTTTGCTGGAAGATGaa > 1:1555366/1‑53 (MQ=255) tcAATAGTGGCTTTACGCTCGACACGTTAGCGTTTCGTTTGCTGGAAGATGAATTGCTGATTGCGCTGCCg > 1:3543271/1‑71 (MQ=255) cAATAGTGGCTTTACGCTCGACACGTTAGCGTTTCGTTTGCTGGAAGATGAATTGCTGATTGCGCTGCCgg > 1:2729569/1‑71 (MQ=255) | TTTTTCCAGCGTCTGTTTGGCAAGGATAATAAGCCAGCTATCGCTCGTGGTCCGCTGGGACTTCATCTCAATAGTGGCTTTACGCTCGACACGTTAGCGTTTCGTTTGCTGGAAGATGAATTGCTGATTGCGCTGCCGG > W3110S.gb/4415993‑4416131 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |