Predicted mutation
evidence seq id position mutation freq annotation gene description
MC JC W3110S.gb 1,301,041 Δ1,336 bp 100% insCinsD insC, insD

Missing coverage evidence...
   seq id start end size ←reads reads→ gene description
* * ÷ W3110S.gb 1301041–1302370 1302376 7–1336 29 [0] [0] 30 insC–insD insC,insD

New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? W3110S.gb = 13010400 (0.000)22 (0.950) 15/84 NT 100% intergenic (+218/‑90) ychE/insC predicted inner membrane protein/IS2 element protein
?W3110S.gb 1302377 = 0 (0.000)intergenic (+19/‑519) insD/oppA IS2 element protein/oligopeptide transporter subunit

CTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTA‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  W3110S.gb/1300970‑1301040
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑gatattaTTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAATAATAATCAATTGTTAAATTATTGTGCATTT  >  W3110S.gb/1302377‑1302443
                                                                                                                                          
CTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATAT                                                                       <  1:674877/69‑1
      ACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATT                                                               >  1:173249/1‑71
      ACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATT                                                               >  1:170141/1‑71
        CATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGA                                                                          <  1:2183691/58‑1
          TCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCAC                                                           >  1:716498/1‑71
          TCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGA                                                                          <  1:1464962/56‑1
            ATGTTATTTTCGCCACATCATAATCCTGGGGTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTG                                                         >  1:149668/1‑71
                 ATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGA                                                                          >  1:1038393/1‑49
                    TTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGG                                                  >  1:666145/1‑70
                      CGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTA                                                <  1:2412398/70‑1
                            ATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATT                                                                   <  1:2522012/45‑1
                             TCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTC                                                              <  1:2047651/49‑1
                              CATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGT                                       >  1:1577031/1‑71
                               ATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTG                                      >  1:1780474/1‑71
                               ATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAA                                                                 >  1:1614431/1‑44
                                TAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGCAGAGGAA                                          <  1:1818886/66‑1
                                TAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGG                                                  <  1:631329/58‑1
                                     CTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAA                                 >  1:1902480/1‑70
                                     CTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTA                                                <  1:94388/55‑1
                                       GGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAA                                 <  1:1664840/68‑1
                                       GGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAA                                 <  1:1644724/68‑1
                                           TTGCTGAAGATTAATTGAAATGATATTATTTATTCCACTGCCTTTGGTt                                                <  1:2504637/49‑2
                                           TTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAATAATAAT                          >  1:1526878/1‑71
                                                 AAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAA                                          <  1:1985474/49‑1
                                                 AAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAA                                          >  1:2228263/1‑49
                                                 AAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAA                                          <  1:1439272/49‑1
                                                 AAGAATAATTGAAATGATATTATTAATTCCACTGCCTTT                                                    <  1:1032038/39‑1
                                                    AATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTA                                                <  1:1305784/40‑1
                                                             AATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAATAATAATCAATTGTTAAATTATTGT        <  1:937830/71‑1
                                                                 ATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAATAATAATCAATTGTTAAATTATTGTGCA     <  1:1681612/70‑1
                                                                 ATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAATAATAATCA                        <  1:2425220/51‑1
                                                                   ATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAATAATAATCAATTGTTAAATTAT           >  1:2018840/1‑62
                                                                    TTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAATAATAATCAATTGTTAAATTATTGTGCATTT  <  1:2155859/70‑1
                                                                                                                                          
CTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTA‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  W3110S.gb/1300970‑1301040
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑gatattaTTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAATAATAATCAATTGTTAAATTATTGTGCATTT  >  W3110S.gb/1302377‑1302443

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.