Predicted mutation
evidence seq id position mutation freq annotation gene description
MC JC W3110S.gb 2,320,795 Δ1,336 bp 100% insD[rcsC] insD, insC, [rcsC]

Missing coverage evidence...
   seq id start end size ←reads reads→ gene description
* * ÷ W3110S.gb 2320795–2322125 2322130 6–1336 43 [0] [0] 41 insD–[rcsC] insD,insC,[rcsC]

New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? W3110S.gb = 23207940 (0.000)23 (0.970) 19/86 NT 100% intergenic (‑59/+11) rcsC/insD hybrid sensory kinase in two‑component regulatory system with RcsB and YojN/IS2 element protein
?W3110S.gb 2322131 = 0 (0.000)coding (2368/2424 nt) rcsC hybrid sensory kinase in two‑component regulatory system with RcsB and YojN

CCACGACCAGAATCATCATATCGTCATTATCGCTGACCGCTTTGTCCGTTGACGGCAGAGCGTTAGCAGGATCGT‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  W3110S.gb/2320720‑2320794
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑gatcgtCGCTCTCCATCTCGATCAAATAAATACGCGCCAACAATGCCGGTAGCTCATGCGGAGCAGCCACACTGTG  >  W3110S.gb/2322131‑2322200
                                                                                                                                                 
CCACGACCAGAATCATCATATCGTCATTATCGCTGACCGCTTTGTCCGTTGACGGCAGAGCGTTAGCAGGA                                                                            >  1:377295/1‑71
    GACCAGAATCATCATATCGTCATTATCGCTGACCGCTTTGTCCGTTGACGGCAGAGCGTTAGCAGGATCGT                                                                        <  1:1110817/71‑1
     ACCAGAATCATCATATCGTCATTATCGCTGACCGCTTTGTCCGTTGACGGCAGAGCGTTAGCAGGATCGT                                                                        >  1:1818722/1‑70
         GAATCATCATATCGTCATTATCGCTGACCGCTTTGTCCGTTGACGGCAGAGCGTTAGCAGGATCGTCGCTC                                                                   <  1:1029324/71‑1
         GAATCATCATATCGTCATTATCGCTGACCGCTTTGTCCGTTGACGGCAGAGCGTTAGCAGGATCGTCGCTC                                                                   <  1:2382756/71‑1
          AATCATCATATCGTCATTATCGCTGACCGCTTTGTCCGTTGACGGCAGAGCGTTAGCAGGATCGTCGC                                                                     <  1:661126/68‑1
            TCATCATATCGTCATTATCGCTGACCGCTTTGTCCGTTGACGGCAGAGCGTTAGCAGGATCGTCGCTCTCC                                                                <  1:1109737/71‑1
            TCATCATATCGTCATTATCGCTGACCGCTTTGTCCGTTGACGGCAGAGCGTTAGCAGGATCGTCGCTCTCC                                                                <  1:1538363/71‑1
                CATATCGTCATTATCGCTGACCGCTTTGTCCGTTGACGGCAGAGCGTTAGCAGGATCGTCGCTCTCCATCT                                                            >  1:1010254/1‑71
                  TATCGTCATTATCGCTGACCGCTTTGTCCGTTGACGGCAGAGCGTTAGCAGGATCGTCGCT                                                                    <  1:2324993/61‑1
                  TATCGTCATTATCGCTGACCGCTTTGTCCGTTGACGGCAGAGCGTTAGCAGGATCGTCGCT                                                                    <  1:2435012/61‑1
                    TCGTCATTATCGCTGACCGCTTTGTCCGTTGACTGCAGAGCGTTAGCAGGATCGTCGCTCTCCATCTCGAT                                                        >  1:468842/1‑71
                      GTCATTATCGCTGACCGCTTTGTCCGTTGACGGCAGAGCGTTAGCAGGATCGTCGCTCTCCATCTCGATCA                                                      <  1:433680/71‑1
                       TCATTATCGCTGACCGCTTTGTCCGTTGACGGCAGAGCGTTAGCAGGATCGTCGCTCTCCATCTC                                                           <  1:2426174/65‑1
                       TCATTATCGCTGACCGCTTTGTCCGTTGACGGCAGAGCGTTAGCAGGAT                                                                           <  1:565205/49‑1
                        CATTATCGCTGACCGCTTTGTCCGTTGACGGCAGAGCGTTAGCAGGATCGTCGCTCTCCATCTCGATCAAA                                                    >  1:2479707/1‑71
                          TTATCGCTGACCGCTTTGTCCGTTGACGGCAGAGCGTTAGCAGGATCGTCG                                                                      <  1:597069/51‑1
                            ATCGCTGACCGCTTTGTCCGTTGACGGCAGCGCGTTAGCAGGATCGTC                                                                       >  1:1440774/1‑48
                            ATCGCTGACCGCTTTGTCCGTTGACGGCAGAGCGTTAGCAGGATCGTCGCTCTCCATCTCGATCAAATAA                                                 <  1:2536282/70‑1
                            ATCGCTGACCGCTTTGTCCGTTGACGGCAGAGCGTTAGCAGGATCGTCGCTCTCCATCTCGATCAAATAA                                                 <  1:882946/70‑1
                            ATCGCTGACCGCTTTGTCCGTTGACGGCAGAGCGTTAGCAGGATAGTCGCTCTCCATCTCGATCAAATAAA                                                >  1:1495419/1‑71
                            ATCGCTGACAGCTTTGTCCGTTGACGGCAGAGCGTTAGCAGGATCGTCGCTCTCCATCTCGATCAAATAAA                                                >  1:1490078/1‑71
                              CGCTGACCGCTTTGTCCGTTGACGGCAGAGCGTTAGCAGGATCGTCGCTCTCCATCTCGATC                                                       >  1:332957/1‑62
                               GCTGACCGCTTTGTCCGTTGACGGCAGAGCGTTAGCAGGATCGTCGCTCTCCATCTCGATCAAATAAATAC                                             >  1:1094488/1‑71
                                     CGCTTTGTCCGTTGACGGCAGAGCGTTAGCAGGATCGTCGCTCTCCATCTCG                                                          <  1:41415/52‑1
                                       CTTTGTCCGTTGACGGCAGAGCGTTAGCAGGATCGTCGCTCTCCATCTCGATCAAA                                                    <  1:45432/56‑1
                                         TTGTCCGTTGACGGCAGAGCGTTAGCAGGATCGTCGCTCTCCATCTCGATCAAATAAA                                                >  1:2415235/1‑58
                                          TGTCCGTTGACGGCAGAGCGTTAGCAGGATCGTCGCTCTCCATCTCGATCAAATAAATACGCGCCAACAA                                   <  1:2138254/70‑1
                                                TTGACGGCAGAGCGTTAGCAGGATCGTCGCTCTCCATCTCGATCAAATAAATACGCGCCAAC                                     >  1:2149686/1‑62
                                                  GACGGCAGAGCGTTAGCAGGATCGTCGCTCTCCATCTCGATCAAATAAATACGCGCCAACAATGCCGGTAG                          >  1:1467285/1‑71
                                                        AGAGCGTTAGCAGGATCGTCGCTCTCCATCTCGATCAAATAAATACGCGCCAACAATGCCGGTAGCTCATG                    <  1:904837/71‑1
                                                           GCGTTAGCAGGATCGTCGCTCTCCATCTCGATCAAATAAATACGCGCCAACAATGCCGGTAGCTCATGCGG                 <  1:457643/71‑1
                                                              TTAGGAGGATCGTCGCTCTCCATCTCGATCAAATAAATACGCGCCAACAATGCCGGTAGCTCATGCGGAGC              <  1:945659/71‑1
                                                              TTAGCAGGATCGTCGCTCTCCATCTCGATCAAATAAATACGCGCCAACAATGCCGGTAGCTCATGCGGAGC              <  1:84877/71‑1
                                                                AGCAGGATCGTCGCTCTCCATCTCGATCAAATAAATACGCGCCAACAATGCCGGTAGCTCATGCGGAGCAG            >  1:2452428/1‑71
                                                                AGCAGGATCGTCGCTCTCCATCTCGATCAAATAAATACGCGCCAACAATGCCG                              >  1:2506541/1‑53
                                                                 GCAGGATCGTCGCTCTCCATCTCGATCAAATAAATACGCGCCAACAATGCCGGTAGCTCATGCGGAGCAGC           <  1:22366/71‑1
                                                                  CAGGATCGTCGCTCTCCATCTCGATCAAATAAATACGCGCCAACAATGCCGGTAGCTCATGCGGAGCAGC           >  1:1302148/1‑70
                                                                    GGATCGTCGCTCTCCATCTCGATCAAATAAATACGCGCCAACAATGCCGGTAGCTCATGCGGAGCAGCCAC        <  1:1404801/71‑1
                                                                    GGATCGTCGCTCTCCATCTCGATCAAATAAATACGCGCCAACAATGCCGGTAGCTCATGCG                  >  1:2598900/1‑61
                                                                     GATCGTCGCTCTCCATCTCGATCAAATAAATACGCGCCAACAATGC                                <  1:2391086/46‑1
                                                                       TCGTCGCTCTCCATCTCGATCAAATAAATACGCGCCAACAATGCCGGTAGCTCATGCGGAGCAGCCACACT     >  1:293496/1‑71
                                                                       TCGTCGCTCTCCATCTCGATCAAATAAATACGCGCCAACAATGCCGGTAGCTCATGCGGAGCAGCCACACT     >  1:242345/1‑71
                                                                       TCGTCGCTCTCCATCTCGATCAAATAAATACGCGCCAACAAT                                  >  1:1149742/1‑42
                                                                          TCGCTCTCCATCTCGATCAAATAAATACGCGCCAACAATGCCGGTAGCTCATGCGGAGCAGCCACACTGTG  <  1:1542061/71‑1
                                                                                                                                                 
CCACGACCAGAATCATCATATCGTCATTATCGCTGACCGCTTTGTCCGTTGACGGCAGAGCGTTAGCAGGATCGT‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  W3110S.gb/2320720‑2320794
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑gatcgtCGCTCTCCATCTCGATCAAATAAATACGCGCCAACAATGCCGGTAGCTCATGCGGAGCAGCCACACTGTG  >  W3110S.gb/2322131‑2322200

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.