Predicted mutation
evidence seq id position mutation freq annotation gene description
MC JC W3110S.gb 3,261,134 Δ1,199 bp 100% insH[tdcD] insH, [tdcD]

Missing coverage evidence...
   seq id start end size ←reads reads→ gene description
* * ÷ W3110S.gb 3261134–3262329 3262332 4–1199 25 [0] [0] 26 insH–[tdcD] insH,[tdcD]

New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? W3110S.gb = 32611330 (0.000)15 (0.600) 12/90 NT 100% intergenic (‑59/‑69) tdcE/insH pyruvate formate‑lyase 4/2‑ketobutyrate formate‑lyase/IS5 element protein
?W3110S.gb 3262333 = 0 (0.000)coding (1183/1296 nt) tdcD propionate kinase/acetate kinase C, anaerobic

TCTACCTTCATGAAAAATAATCTCTCTACAATACTTCAACTAAATTATGCAAATTCTGCGGGCGCGTTAA‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  W3110S.gb/3261064‑3261133
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑ttaaCTTTGCCTAAATGAATGGCATCCAAAGCAATCATTTTTTCTTCGTTAGTCGGAATAACGGCACAAATGA  >  W3110S.gb/3262333‑3262401
                                                                                                                                            
TCTACCTTCATGAAAAATAATCTCTCTACAATACTTCAACTAAATTATGCAAATTCTGCGGGCGCGTTAAC                                                                       >  1:1733179/1‑71
TCTACCTTCATGAAAAATAATCTCTCTACAATACTTCAACTAAATTATGCAAATTCTGCGGGCGCGTTAAC                                                                       >  1:400856/1‑71
  TACCTTCATGAAAAATAATCTCTCTACAATACTTCAACTAAATTATGCAAATTCTGCGGGCGCGT                                                                           >  1:1884423/1‑65
       TCATGAAAAATAATCTCTCTACAATACTTCAACTAAATTATGGAAATTCTGCGGGCGCGTTA                                                                         >  1:1401684/1‑62
       TCATGAAAAATAATCTCTCTACAATACTTCAACTAAATTATGCAAATTCTGCGGGCGCGTTAACTTTGCC                                                                >  1:762432/1‑70
                        TCTACAATACTTCAACTAAATTATGCAAATTCTGCGGGCGCGTTAACTTTGCCTAAATGAATGGCATCC                                                <  1:1406164/69‑1
                         CTACAATACTTCAACTAAATTATGCAAATTCTGCGGGCGCGTTAACTTTGCCTAAATGAATGGCATCCA                                               >  1:2503338/1‑69
                            CAATACTTCAACTAAATTATGCAAATTCTGCGGGCGCGTTAACTTTTGCCTAAATGAATGGCATCCAAAG                                            >  1:1011893/1‑70
                            CAATACTTCAACTAAATTATGCAAATTCTGCGGGCGCGTTAACTTT                                                                   <  1:2289845/46‑1
                             AATACTTCAACTAAATTATGCAAATTCTGCGGGCGCGTTAACTTTGCCTAAAT                                                           <  1:422798/53‑1
                               TACTTCAACTAAATTATGCAAATTCTGCGGGCGCGTTAACTTTGCCTAAATGAATGGCATCCAAAGCAAT                                        >  1:1727615/1‑70
                                ACTTCAACTAAATTATGCAAATTCTGCGGGCGCGTTAACTTTGCCTAAATGAATGGCATCCAAAGCAATCA                                      >  1:1720237/1‑71
                                ACTTCAACTAAATTATGCAAATTCTGCGGGCGCGTTAACTTTGCCTAAATGAATGGCATCCAAAGCAATCA                                      >  1:1817030/1‑71
                                 CTTCAACTAAATTATGCAAATTCTGCGGGCGCGTTAACTTTGCCTAAATGAA                                                        <  1:1258681/52‑1
                                       CTAAATTATGCAAATTCTGCGGGCGCGTTAACTTTGCCTAAATGAATGGCATCCAAAGCAATCATTTTTTC                               <  1:256775/71‑1
                                         AAATTATGCAAATTCTGCGGGCGCGTTAACTTTGCCTAAA                                                            >  1:1748114/1‑40
                                         AAATTATGCAAATTCTGCGGGCGCGTTAACTTTGCCTAAA                                                            >  1:1393875/1‑40
                                            TTATGCAAATTCTGCGGGCGCGTTAACTTTGCCTAAATGAA                                                        <  1:1146210/41‑1
                                               TGCAAATTCTGCGGGCGCGTTAACTTTGCCTAAATGAATGGCATCCAAAGCAATCATTTTTTCTTCGTTA                        <  1:14678/70‑1
                                                   AATTCTGCGGGCGCGTTAACTTTGCCTAAATGAATGGCATCCAAAGCAATCATTTTTTCTTCGTTAGTCG                    >  1:2083279/1‑70
                                                              CGCGTTAACTTTGCCTAAATGAATGGCATCCAAAGCAATCATTTTTTCT                              >  1:1323382/1‑49
                                                                 GTTAACTTTGCCTAAATGAATGGCATCCAAAGCAATCATTTTTTCTTCGTTAGTCGGAAT                <  1:1544555/60‑1
                                                                  TTAACTTTGCCTAAATGAATGGCATCCAAAGCAATCATTTTTTCTTCTTTAGTCGGAATAAC             <  1:1495410/62‑1
                                                                  TTAACTTTGCCTAAATGAATGGCATCCAAAGCAATCATTTTTTCTTCGTTAGTCGGAATAACGGCACA       <  1:427066/68‑1
                                                                   TAACTTTGCCTAAATGAATGGCATCCAAAGCAATCATTTTTTCTTCGTTAGTCGGAATAACGGCACAAAT    >  1:780345/1‑70
                                                                    AACTTTGCCTAAATGAATGGCATCCAAAGCAATCATTTTTTCTTCGTTAGTCGGAATAACGGCACAAATGA  >  1:1782576/1‑71
                                                                    AACTTTGCCTAAATGAATGGCATCCAAAGCAATCATATTTTCTTCG                           >  1:1998005/1‑46
                                                                                                                                            
TCTACCTTCATGAAAAATAATCTCTCTACAATACTTCAACTAAATTATGCAAATTCTGCGGGCGCGTTAA‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  W3110S.gb/3261064‑3261133
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑ttaaCTTTGCCTAAATGAATGGCATCCAAAGCAATCATTTTTTCTTCGTTAGTCGGAATAACGGCACAAATGA  >  W3110S.gb/3262333‑3262401

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.