| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 2,154 | C→T | 47.6% | D606D (GAC→GAT) | thrA → | fused aspartokinase I and homoserine dehydrogenase I |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,154 | 0 | C | T | 47.6% | ‑1.4 / 26.9 | 21 | D606D (GAC→GAT) | thrA | fused aspartokinase I and homoserine dehydrogenase I |
| Reads supporting (aligned to +/- strand): ref base C (3/8); new base T (7/3); total (10/11) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 8.61e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GATGGATTACTACCATCAGTTGCGTTATGCGGCGGAAAAATCGCGGCGTAAATTCCTCTATGACACCAACGTTGGGGCTGGATTACCGGTTATTGAGAACCTGCAAAATCTGCTCAATGCAGGT > W3110S.gb/2091‑2214 | gATGGATTACTACCATCAGTTGCGTTATGCGGCGGAAAAATCGCGGCGTAAATTCCTCTATGATACCAACg > 1:2310534/1‑71 (MQ=255) aTGGATTACTACCATCAGTTGCGTTATGCGGCGGAAAAATCGCGGCGTAAATTCCTCTATGACACCAACGt < 1:810324/71‑1 (MQ=255) tactacAATCAGTTGCGTTATGCGGCGGAAAAATCGCGGCGTAAATTCCTCTATGATACCAACGTTGGGGc > 1:2493500/1‑71 (MQ=255) aGTTGCGTTATGCGGCGGAAAAATCGCGGCGTAAATTCCTCTATGATACCAACGTTGGGGCTGGATTACCg > 1:505437/1‑71 (MQ=255) gTTGCGTTATGCGGCGGAAAAATCGCGGCGTAAATTCCTCTATGATACCAACGTTgg < 1:916119/57‑1 (MQ=255) ttGCGTTATGCGGCGGAAAAATCGCGGCGTAAATTCCTCTATGACACCaa < 1:165933/50‑1 (MQ=255) aaaaTCGCGGCGTAAATTCCTCTATGATACCAACGTTg > 1:155367/1‑38 (MQ=255) aaaaTCGCGGCGTAAATTCCTCTATGACACCAACGTTGGGGCTGGATTACCGGTTATTGAGAACCTGCaaa < 1:778308/71‑1 (MQ=255) aaaTCGCGGCGTAAATTCCTCTATGATACCAACGTTGGGGCTGGAt > 1:972679/1‑46 (MQ=255) aaaTCGCGGCGTAAATTCCTCTATGATACCAACGTTGGGGCTGGAt > 1:2158660/1‑46 (MQ=255) aaaTCGCGGCGTAAATTCCTCTATGACACCAACGTTGGGGCTGGATTACCGGTTATTGAGAACCTGCaaaa < 1:1543658/71‑1 (MQ=255) cgcgGCGTAAATTCCTCTATGACACCAACGTTGGGGCTGGATTACCGGt > 1:1600080/1‑49 (MQ=255) ggcgTAAATTCCTCTATGACACCAACGTTGGGGCTGGATTACCGGTTATTGAGAACCTGCAAAATCTGCTc < 1:2215053/71‑1 (MQ=255) cgTAAATTCCTCTATGATACCAACGTTGGGGCTGGATTACCGGTTATTg < 1:933139/49‑1 (MQ=255) aaaTTCCTCTATGACACCAACGTTGGGGCTGGATTACCGGTTATTgaga > 1:472046/1‑49 (MQ=255) aaTTCCTCTATGACACCAACGTTGGGGCTGGATTACCGGTTATTGAGAACCTGCAAAATCTGCTCAATg > 1:1135721/1‑69 (MQ=255) aTTCCTCTATGATACCAACGTTGGGGCTGTATTACCGGTTAtt < 1:1187810/43‑1 (MQ=38) aTTCCTCTATGACACCAACGTTGGGGCTGGATTACCGGTTATTGAGAACCTGCaaa < 1:2565892/56‑1 (MQ=255) ttCCTCTATGATACCAACGTTGGGGCTGGATTACCGGTTATT‑AGAACCTGCAAAATCt > 1:651702/1‑58 (MQ=255) ccTCTATGACACCAACGTTGGGGCTGGATTACCGGTTATTGAGAACCTGCAAAATCTGCTCAATGCAGGt < 1:1466657/70‑1 (MQ=255) tGACACCAACGTTGGGGCTGGATTACCGGTTATTGAGAACCTGCaaa < 1:2222700/47‑1 (MQ=255) | GATGGATTACTACCATCAGTTGCGTTATGCGGCGGAAAAATCGCGGCGTAAATTCCTCTATGACACCAACGTTGGGGCTGGATTACCGGTTATTGAGAACCTGCAAAATCTGCTCAATGCAGGT > W3110S.gb/2091‑2214 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |