| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 155,463 | A→T | 100% | *247K (TAA→AAA) | ecpD ← | predicted periplasmic pilin chaperone |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 155,463 | 0 | A | T | 100.0% | 157.3 / NA | 53 | *247K (TAA→AAA) | ecpD | predicted periplasmic pilin chaperone |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base T (30/23); total (30/23) | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
TGATGATAATTTTTAGTATATTCTATAGTCACGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTACAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCATTAATGGCGTAAAAATGCACTTTTGCAGAA > W3110S.gb/155395‑155530 | tgatgatAATTTTTAGTATATTCTATAGTCACGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCa < 1:180916/71‑1 (MQ=255) gatgatAATTTTTAGTATATTCTATAGTCACGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAg > 1:1970984/1‑71 (MQ=255) atgatAATTTTTAGTATATTCTATAGTCACGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGc > 1:721242/1‑71 (MQ=255) tgatAATTTTTAGTATATTCTATAGTCACGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGcc > 1:1896633/1‑71 (MQ=255) gatAATTTTTAGTATATTCTATAGTCACGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCt > 1:826225/1‑71 (MQ=255) tttAGTATATTCTATAGTCACGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCAtt < 1:1298974/70‑1 (MQ=255) tttAGTATATTCTATAGTCACGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTa > 1:257696/1‑71 (MQ=255) tatTCTATAGTCACGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCt < 1:1031352/57‑1 (MQ=255) tatTCTATAGTCACGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCaa > 1:459371/1‑71 (MQ=255) tatTCTATAGTCACGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCaa > 1:301917/1‑71 (MQ=255) atTCTATAGTCACGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCa < 1:188549/52‑1 (MQ=255) atTCTATAGTCACGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCa < 1:1808070/52‑1 (MQ=255) ttCTATAGTCACGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAAt > 1:1028843/1‑70 (MQ=255) tCTATAGTCACGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTg > 1:90787/1‑71 (MQ=255) tCTATAGTCACGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTg > 1:1609061/1‑71 (MQ=255) cTATAGTCACGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCa > 1:1013135/1‑57 (MQ=255) cTATAGTCACGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCa > 1:1992084/1‑57 (MQ=255) cTATAGTCACGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTg < 1:1581305/70‑1 (MQ=255) gTCACGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTg > 1:1734837/1‑49 (MQ=255) gTCACGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTg > 1:1311971/1‑49 (MQ=255) gTCACGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCtt > 1:2436189/1‑59 (MQ=255) tCACGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTgg < 1:272388/49‑1 (MQ=255) tCACCCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTc < 1:1671342/59‑1 (MQ=255) aCGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTAc > 1:2011260/1‑53 (MQ=255) aCGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTAc > 1:649911/1‑53 (MQ=255) aCGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGccacca < 1:831311/70‑1 (MQ=255) gCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAg > 1:658207/1‑71 (MQ=255) cTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGt < 1:462173/71‑1 (MQ=255) cTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGt < 1:2609710/71‑1 (MQ=255) aTGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCa > 1:625575/1‑71 (MQ=255) tGCTTCCTGCGGAATTATATCCTTGCCTGATTTGAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCAt < 1:2642027/71‑1 (MQ=255) tGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCt > 1:692677/1‑39 (MQ=255) cTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCAtt > 1:1084901/1‑70 (MQ=255) cTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCAtt < 1:1717807/70‑1 (MQ=255) ttCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTAc > 1:892096/1‑44 (MQ=255) ccTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCATTaa < 1:1982808/69‑1 (MQ=255) ccTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCATTAAt < 1:1468919/70‑1 (MQ=255) ccTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCATTAATg > 1:1662146/1‑71 (MQ=255) cGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTgcg < 1:2054371/49‑1 (MQ=255) aaTTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCATTAATGGCGTaaa < 1:506754/71‑1 (MQ=255) aaTTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCATTAATGGCGTaa > 1:1725865/1‑70 (MQ=255) tataTCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGccac < 1:2063735/47‑1 (MQ=255) atatCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCATTAATGGCGTaaaaa > 1:2033752/1‑69 (MQ=255) atatCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCATTAATGGCGTAAAAATg > 1:1586947/1‑71 (MQ=255) atatCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCATTAATGGCGTAAAAATg > 1:1965186/1‑71 (MQ=255) tatCCTTGCCTGATTTCAGCCTGGCATTACCTTCa < 1:2416290/35‑1 (MQ=255) tatCCTTGCCTGATTTCAGCCTGGCATTACCTTCa < 1:2403387/35‑1 (MQ=255) cTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCaaa > 1:374438/1‑45 (MQ=255) cTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCATTAATGGCGTAAAAATGCACtt > 1:1210153/1‑71 (MQ=255) ccTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCATTAATGGCGTAAAAATGCACTTTTg < 1:2409329/70‑1 (MQ=255) gATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCATTaa > 1:845981/1‑46 (MQ=255) aTTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCATTAATGGCGTAAAAATGCACTTTTGCAGaa < 1:1226643/71‑1 (MQ=255) aTTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCATTAATGGCGTAAAAATGCACTTTTGCAGaa < 1:191658/71‑1 (MQ=255) | TGATGATAATTTTTAGTATATTCTATAGTCACGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTACAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCATTAATGGCGTAAAAATGCACTTTTGCAGAA > W3110S.gb/155395‑155530 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |