| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 810,113 | G→A | 26.1% | M116I (ATG→ATA) | bioB → | biotin synthase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 810,113 | 0 | G | A | 26.1% | 38.3 / 11.7 | 23 | M116I (ATG→ATA) | bioB | biotin synthase |
| Reads supporting (aligned to +/- strand): ref base G (5/12); new base A (3/3); total (8/15) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.21e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.70e-01 | |||||||||||
GCGCTTCTGTATGGGCGCGGCGTGGAAGAATCCCCACGAACGCGATATGCCGTACCTGGAACAAATGGTGCAGGGGGTAAAAGCGATGGGGCTGGAGGCGTGTATGACGCTGGGCACGTTGAGTGAAT > W3110S.gb/810047‑810174 | gcgcTTCTGTATGGGCGCGGCGTGGAAGAATCCCCACGAACGCGATATGCCGTACCTGGAACAAATAGTGc < 1:921584/71‑1 (MQ=255) cgcTTCTGTATGGGCGCGGCGTGGAAGAATCCCCACGAACGCGATATGCCGTACCTGGAACAAATGGTGCa < 1:1141664/71‑1 (MQ=255) cTTCTGTATGGGCGCGGCGTGGAAGAATCCCCACGAACGCGATATGCCGTACCTGGAACAAATGGTGCAg < 1:96307/70‑1 (MQ=255) aTGGGCGCGGCGTGGAAGAATCCCCACGAACGCGATATGCCGTACCTGGAACAAATGGTGCAgg > 1:1123202/1‑64 (MQ=255) aTGGGCGCGGCGTGGAAGAATCCCCACGAACGCGATATGCCGTACCTGGAACAAATGGTGCAg > 1:546169/1‑63 (MQ=255) tGGGCGCGGCGTGGAAGAATCCCCACGAACGCGATATGCCGTACCTGGAACAAATGGTGCAGGGGGTaa > 1:1180209/1‑69 (MQ=255) ggCGCGGCGTGGAAGAATCCCCACGAACGCGATATGCCGTACCTGGAACAAATGGTGCAGGGGGTAAAAGc < 1:278710/71‑1 (MQ=255) gaagaaTCCCCACGAACGCGATATGCCGTACCTGGAACAAATGGTGCAGGGGGTAAAAGc < 1:252689/60‑1 (MQ=255) gaagaaTCCCCACGAACGCGATATGCCGTACCTGGAACAAATGGTGCAGGGGGTAAAAGCGATGGGGCTg < 1:1047039/70‑1 (MQ=255) aagaaTCCCCACGAACGCGATATGCCGTACCTGGAACAAATGGTGCAGGGGGTAAAAGCGATGGGGCTg > 1:590701/1‑69 (MQ=255) aaTCCCCACGAACGCGATATGCCGTACCTGGAACAAATGGTGCAGGGGGTAAAAGCGATGGGGCTGGAGGc < 1:2384780/71‑1 (MQ=255) tCCCCACGAACGCGATATGCCGTACCTGGAACAAATAGTGCAGGGGGTAAAAGc < 1:2564204/54‑1 (MQ=255) cccACGAACGCGATATGCCGTACCTGGAACAAATGGTGCAGGGGGTAAAAGCGATGGGGCTGGAGGCgtgt < 1:621767/71‑1 (MQ=255) aCGAACGCGATATGCCGTACCTGGAACAAATGGTGCAGGGGGTAAAAGCGATGGGGCTGGAGGCGTGTATg < 1:1326373/71‑1 (MQ=255) aCGCGATATGCCGTACCTGGAACAAATGGTGCAGGGGGTAAAAGCGATGGGGCTGGAGGCGTGTATGACGc < 1:2211950/71‑1 (MQ=255) atatACCGTACCTGGAACAAATGGTGCAGGGGGTAAAAGCGATGGGGCTGGAGGCGTGTATGACGCTggg < 1:1794658/70‑1 (MQ=255) tatGCCGTACCTGGAACAAATAGTGCAGGGGGTAAAAGCGATGGGGCTGGAGGCGTGTATGACGCTGGGCa < 1:2214039/71‑1 (MQ=255) atGCCGTACCTGGAACAAATGGTGCAGGGGGTAAAAGCGa > 1:78532/1‑40 (MQ=255) cGTACCTGGAACAAATAGTGCAGGGGGTAAAAGAGATGGGGCTGGAGGCGTGTa > 1:482634/1‑54 (MQ=255) cTGGAACAAATGGTGCAGGGGGTAAAAGCGATGGGGCTGGAGGCGTGTATGACGCTGGGCACGTTg < 1:428022/66‑1 (MQ=255) ggAACAAATAGTGCAGGGGGTAAAAGCGATGGGGCTGGAGGCGTGTATGACGCTGGGCACGTTGa > 1:1722510/1‑65 (MQ=255) aaTAGTGCAGGGGGTAAAAGCGATGGGGCTGGAGGCGTGTATGACGCTGGGCACGTTGAGTGAAt > 1:1652942/1‑65 (MQ=255) aTGGTGCAGGGGGTAAAAGCGATGGGGCTGGAGGCGTGTATGACGCTGGGCACGTTGAGTGa < 1:362414/62‑1 (MQ=255) | GCGCTTCTGTATGGGCGCGGCGTGGAAGAATCCCCACGAACGCGATATGCCGTACCTGGAACAAATGGTGCAGGGGGTAAAAGCGATGGGGCTGGAGGCGTGTATGACGCTGGGCACGTTGAGTGAAT > W3110S.gb/810047‑810174 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |