Predicted mutation
evidence seq id position mutation freq annotation gene description
RA W3110S.gb 907,713 G→A 35.7% T331I (ACC→ATC)  ybjT ← conserved hypothetical protein with NAD(P)‑binding Rossmann‑fold domain

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb907,7130GA35.7% 8.9 / 9.7 14T331I (ACC→ATC) ybjTconserved hypothetical protein with NAD(P)‑binding Rossmann‑fold domain
Reads supporting (aligned to +/- strand):  ref base G (7/2);  new base A (2/3);  total (9/5)
Fisher's exact test for biased strand distribution p-value = 2.66e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.81e-01

CGCTCTTTACCGCCGATTTGGTTCACTACCTGCCATAAAGCAGCAAGGCTGGCGGACGTTTTAACGGTAAACCCCGCCTGTTTGGCAAAATAACCGTACTCCGGTCGCCAGCGGGCAAAGGCCTGAGCGTCGT  >  W3110S.gb/907647‑907779
                                                                  |                                                                  
cGCTCTTTACCGCCGATTTGGTTCACTACCTGCCATAAAGCAGCAAGGCTGGCGGACGTTTTAACGATaaa                                                                <  1:2171739/71‑1 (MQ=255)
 gCTCTTTACCGCCGATTTGGTTCACTACCTGCCATAAAGCAGCAAGGCTGGCGGACGTTTTAACGGTAAAc                                                               >  1:1032322/1‑71 (MQ=255)
  ctctTTACCGCCGATTTGGTTCACTACCTGCCATAAAGCAGCAAGGCTGGCGGACGTTTTAACGGTAAAcc                                                              >  1:1360548/1‑71 (MQ=255)
  ctctTTACCGCCGATTTGGTTCACTACCTGCCATAAAGCAGCAAGGCTGGCGGACGTTTTAACGATAAAcc                                                              >  1:2246436/1‑71 (MQ=255)
                tttGGTTCACTACCTGCCATAAAGCAGCAAGGCTGGCGGACGTTTTAACGGTAAACCCCGc                                                          >  1:1178936/1‑61 (MQ=255)
                            ccTGCCATAAAGCAGCAAGGCTGGCGGACGTTTTAACGGTAAACCCCGCCTGTTTGGCAAAATAACCGTAc                                    >  1:1142455/1‑71 (MQ=255)
                            ccTGCCATAAAGCAGCAAGGCTGGCGGACGTTTTAACGGTAAACCCCGCCTGTTTGGCAAAATAACCGTAc                                    >  1:142608/1‑71 (MQ=255)
                                 cATAAAGCAGCAAGGCTGGCGGACGTTTTAACGGTAAACCCCGCCTGTTTGGCAAAATAAc                                         <  1:1452049/61‑1 (MQ=255)
                                     aaGCAGCAAGGCTGGCGGACGTTTTAACGGTAAACCCCGCCt                                                        >  1:863901/1‑42 (MQ=255)
                                     aaGCAGCAAGGCTGGCGGACGTTTTAACGGTAAACCCCGCCTGTTTGGCAAAATAACCGTACTCCGGTCGc                           <  1:1306764/71‑1 (MQ=255)
                                                         gTTTTAACGATAAACCCCGCCTGTTTGGCAAAATAACCg                                       <  1:130093/39‑1 (MQ=255)
                                                            ttAACGATAAACCCCGCCTGTTTGGCAAAATAACCGTa                                     >  1:2169202/1‑38 (MQ=255)
                                                              aaCGGTAAACCCCGCCTGTTTGGCAAAATAACCGTACTCCGGTCGCCAGCGGGCAAAGGCCTGAGcgtcg   >  1:591684/1‑70 (MQ=255)
                                                               aCGATAAACCCCGCCTGTTTGGCAAAATAACCGTACTCCGGTCGCCAGCGGGCAAAGGCCTGAGcgtcgt  <  1:1912052/70‑1 (MQ=255)
                                                                  gTAAACCCCGCCTGTTTGGCAAAATAACCGTACTCCGGt                              >  1:1459455/1‑39 (MQ=255)
                                                                  |                                                                  
CGCTCTTTACCGCCGATTTGGTTCACTACCTGCCATAAAGCAGCAAGGCTGGCGGACGTTTTAACGGTAAACCCCGCCTGTTTGGCAAAATAACCGTACTCCGGTCGCCAGCGGGCAAAGGCCTGAGCGTCGT  >  W3110S.gb/907647‑907779

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑