Predicted mutation
evidence seq id position mutation freq annotation gene description
RA W3110S.gb 70,434 C→T 40.5% N16N (AAC→AAT araC → DNA‑binding transcriptional dual regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb70,4340CT40.5% 24.0 / 41.4 37N16N (AAC→AATaraCDNA‑binding transcriptional dual regulator
Reads supporting (aligned to +/- strand):  ref base C (13/9);  new base T (7/8);  total (20/17)
Fisher's exact test for biased strand distribution p-value = 5.16e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GGAGTATGAAAAGTATGGCTGAAGCGCAAAATGATCCCCTGCTGCCGGGATACTCGTTTAACGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGCCAACGGTTATCTCGATTTTTTTATCGACCGAC  >  W3110S.gb/70373‑70501
                                                             |                                                                   
ggAGTATGAAAAGTATGGCTGAAGCGCAAAATGATCCCCTGCTGCCGGGATACTCGTTTAACGCCCATCt                                                             <  1:1095702/70‑1 (MQ=255)
  aGTATGAAAAGTATGGCTGAAGCGCAAAATGATCCCCTGCTGCCGGGATACTCGTTTAATGCCCATCtggt                                                          <  1:2165483/71‑1 (MQ=255)
    tATGAAAAGTATGGCTGAAGCGCAAAATGATCCCCTGCTGCCGGGATACTCGTTTAATGCCCATCtggtg                                                         <  1:1449037/70‑1 (MQ=255)
        aaaaGTATGGCTGAAGCGCAAAATGATCCCCTGCTGCCGGGATACTCGTTTAATGCCCATCTGGTGGCggg                                                    <  1:1037462/71‑1 (MQ=255)
        aaaaGTATGGCTGAAGCGCAAAATGATCCCCTGCTGCCGGGATACTCGTTTAATGCCCATCTGGTGGCggg                                                    >  1:1336887/1‑71 (MQ=255)
         aaaGTATGGCTGAAGCGCAAAATGATCCCCTGCTGCCGGGATACTCGTTTAATGCCCATCTGGTGGCGGGt                                                   >  1:1890964/1‑71 (MQ=255)
         aaaGTATGGCTGAAGCGCAAAATGATCCCCTGCTGCCGGGATACTCGTTTAACGCCCATCTGGTGGCGGGt                                                   >  1:935687/1‑71 (MQ=255)
         aaaGTATGGCTGAAGCGCAAAATGATCCCCTGCTGCCGGGATACTCGTTTAACGCCCATCTGGTGGCGGGt                                                   <  1:572346/71‑1 (MQ=255)
            gTATGGCTGAAGCGCAAAATGATCCCCTGCTGCCGGGATACTCGTTTAATGCCCATCTGGTGGCGGGttt                                                 >  1:2028136/1‑70 (MQ=255)
                    gAAGCGCAAAATGATCCCCTGCTGCCGGGATACTCGTTTAATGCCCATCTGGTGGCGGGTTTAACGCCGAt                                        >  1:1365542/1‑71 (MQ=255)
                     aaGCGCAAAATGATCCCCTGCTGCCGGGATACTCGTTTAATGCCCATCTGGTGGCGGGTTTAACGCCGAtt                                       <  1:2596800/71‑1 (MQ=255)
                     aaGCGCAAAATGATCCCCTGCTGCCGGGATACTCGTTTAACCCCCATCTGGTGGCGGGTTTAACGCCGAtt                                       <  1:550274/71‑1 (MQ=255)
                         gcAAAATGATCCCCTGCTGCCGGGATACTCGTTTAACGCCCATCTGGTGGCGGGTTTAACGCCGATTGAgg                                   >  1:1161576/1‑71 (MQ=255)
                              aTGATCCCCTGCTGCCGGGATACTCGTTTAACGCCCATCTGGTGGCGGGTTTaa                                               >  1:2216771/1‑54 (MQ=255)
                              aTGATCCCCTGCTGCCGGGATACTCGTTTAACGCCCATCTGGTGGCGGGTTTAACGCCGa                                         >  1:274234/1‑60 (MQ=255)
                                  tCCCCTGCTGCCGGGATACTCGTTTAATGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGCCAACGGtt                          <  1:500527/71‑1 (MQ=255)
                                    cccTGCTGCCGGGATACTCGTTTAACGCCCATCTGGTGGCGGGTTTAACGCCGATTGa                                     >  1:288267/1‑58 (MQ=255)
                                     ccTGCTGACGGGATACTCGTTTAATGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGCCAACGGTTAtc                       <  1:2085718/71‑1 (MQ=255)
                                     catgctgcCGGGATACTCGTTTAACGCCCATCTGGTGGCGGGTTTAACGcc                                           <  1:669412/49‑1 (MQ=255)
                                      ctgctgCCGGGATACTCGTTTAACGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGCCAACGGTTAt                        <  1:1983265/69‑1 (MQ=255)
                                       tgctgcCGGGATACTCGTTTAATGCCCATCTGGTGGCGGGTTTaa                                               >  1:420261/1‑45 (MQ=255)
                                        gctgcCGGGATACTCGTTTAACGCCCATCTGGTGGCGGGTTTAACGCCGAt                                        >  1:1618447/1‑51 (MQ=255)
                                         ctgcCGGGATACTCGTTTAACGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGCCAACGGTTATCTCGa                   >  1:423264/1‑71 (MQ=255)
                                            ccGGGATACTCGTTTAATGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGCCAACgg                            <  1:1011835/59‑1 (MQ=255)
                                              gggATACTCGTTTAACGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGCCAACGGTTAtct                      >  1:1227845/1‑63 (MQ=255)
                                              gggATACTCGTTTAACGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGCCAACGGTTATCTCGAttttt              >  1:1190796/1‑71 (MQ=255)
                                                gATACTCGTTTAACGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGCCAACGGTTATCTCGAttttttt            <  1:1792966/71‑1 (MQ=255)
                                                gATACTCGTTTAACGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGCCAACGGTTATCTCGAttttttt            <  1:1252484/71‑1 (MQ=255)
                                                 aTACTCGTTTAACGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGCCAACGGTTATCTCGATTTTTTTa           >  1:785132/1‑71 (MQ=255)
                                                    cTCGTTTAACGCCCATCTGGTGGCGGGTTTAACGCCGATTGAgg                                   >  1:1017243/1‑44 (MQ=255)
                                                    cTCGTTTAACGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGCCAACGGTTATCTCGATTTTTTTATc         >  1:289548/1‑70 (MQ=255)
                                                     tCGTTTAACGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGCCAACGGTTATCTCGa                   <  1:1591768/59‑1 (MQ=255)
                                                        tttAATGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGCCAACGGTTATCTCGATTTTTTTATcgaccg    <  1:663236/71‑1 (MQ=255)
                                                          tAATGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGCCAACGGTTATCTCGATTTTTTTATcgaccgac  >  1:559223/1‑71 (MQ=255)
                                                          tAATGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGCCAACGGTTATCTCGATTTTTTTATcgaccgac  >  1:2029673/1‑71 (MQ=255)
                                                          tAACGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGCCAACGGTTATCTCGAttttt              >  1:2037941/1‑59 (MQ=255)
                                                           aaCGCCCATCTGGGGGCGGGTTTAACGCCGATTGa                                     <  1:2357810/35‑1 (MQ=255)
                                                             |                                                                   
GGAGTATGAAAAGTATGGCTGAAGCGCAAAATGATCCCCTGCTGCCGGGATACTCGTTTAACGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGCCAACGGTTATCTCGATTTTTTTATCGACCGAC  >  W3110S.gb/70373‑70501

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: