| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 1,303,631 | C→T | 100% | Q246Y (CAG→TAT) | oppA → | oligopeptide transporter subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 1,303,631 | 0 | C | T | 100.0% | 70.7 / NA | 25 | Q246Y (CAG→TAT) | oppA | oligopeptide transporter subunit |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (15/10); total (15/10) | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
ACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATC > W3110S.gb/1303573‑1303696 | acgaaTCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTAt < 1:2397841/70‑1 (MQ=255) cTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGc > 1:1294841/1‑71 (MQ=255) cTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGc > 1:186022/1‑71 (MQ=255) cTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGc > 1:2023208/1‑71 (MQ=255) tGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCtt > 1:2095997/1‑71 (MQ=255) gAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCtt > 1:634554/1‑70 (MQ=255) ccGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTAt > 1:540803/1‑48 (MQ=39) gACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCg < 1:369854/71‑1 (MQ=255) cTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATg > 1:1665504/1‑71 (MQ=255) aCTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGa < 1:689843/67‑1 (MQ=255) cTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCa < 1:776637/71‑1 (MQ=255) ggAACAACGCGAAAACCGTTATTAACTATGTAAcc < 1:2635580/35‑1 (MQ=37) gAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCaa < 1:2440516/69‑1 (MQ=255) gAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAAc > 1:1587057/1‑70 (MQ=255) gAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAAc < 1:401992/70‑1 (MQ=255) acGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTAc > 1:1263769/1‑71 (MQ=255) gcgAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCg > 1:1623223/1‑71 (MQ=255) aaaaCCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTg > 1:701665/1‑43 (MQ=38) aaaaCCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTAcc > 1:1941233/1‑51 (MQ=255) aaCCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGa > 1:1321823/1‑51 (MQ=255) aaCCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACAGATGTCAACCGCTACCGTAgtg < 1:2547199/71‑1 (MQ=255) cGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAgtggtg < 1:1019917/71‑1 (MQ=255) cGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAgtggtg < 1:1013627/71‑1 (MQ=255) tattaACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAg > 1:1964391/1‑63 (MQ=255) taACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATc > 1:1538572/1‑70 (MQ=255) | ACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATC > W3110S.gb/1303573‑1303696 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |