Predicted mutation
evidence seq id position mutation freq annotation gene description
RA W3110S.gb 2,058,034 C→T 37.2% G279G (GGC→GGT amn → AMP nucleosidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb2,058,0340CT37.2% 41.0 / 43.1 43G279G (GGC→GGTamnAMP nucleosidase
Reads supporting (aligned to +/- strand):  ref base C (18/9);  new base T (10/6);  total (28/15)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AAACATCAGATGCCAGCATGGCATTTAATTACCGCCGATGGTCAGGGTATTACTCTGGTGAATATTGGCGTGGGACCGTCAAATGCTAAAACCATCTGCGATCATCTGGCAGTGCTACGCCCGGATGTCTGGTTGAT  >  W3110S.gb/2057966‑2058102
                                                                    |                                                                    
aaaCATCAGATGCCAGCATGGCATTTAATTACCGCCGATGGTCAGGGTATTACTCTGGTGAATATTGGCGt                                                                    >  1:2194027/1‑71 (MQ=255)
      cAGATGCCAGCATGGCATTTAATTACCGCCGATGGTCAGGGTATTACTCTGGTGAATATTGGTGTGGGAcc                                                              >  1:2461406/1‑71 (MQ=255)
       aGATGCCAGCATGGCATTTAATTACCGCCGATGGTCAGGGTATTACTCTGGTGAATATTGGCGTGGGACCg                                                             >  1:1657502/1‑71 (MQ=255)
            ccAGCATGGCATTTAATTACCGCCGATGGTCAGGGTATTACTCTGGTGAATATTGGTGTGGGACCGTCaaa                                                        <  1:1638490/71‑1 (MQ=255)
            ccAGCATGGCATTTAATTACCGCCGATGGTCAGGGTATTACTCTGGTGAATATTGGCGTGGGACCGTCaaa                                                        >  1:1006434/1‑71 (MQ=255)
             cAGCATGGCATTTAATTACCGCCGATGGTCAGGGTATTACTCTGGTGAATATTGGCGTGGGACCGTCaaa                                                        >  1:645799/1‑70 (MQ=255)
             cAGCATGGCATTTAATTACCGCCGATGGTCAGGGTATTACTCTGGTGAATATTGGCGTGGGACCGTCaaa                                                        >  1:1923364/1‑70 (MQ=255)
             cAGCATGGCATTTAATTACCGCCGATGGTCAGGGTATTACTCTGGTGAATATTGGCGTGGGACCGTCaaa                                                        >  1:1768251/1‑70 (MQ=255)
                  tGGCATTTAATTACCGCCGATGGTCAGGGTATTACTCTGGTGAATATTGGc                                                                      <  1:2538250/51‑1 (MQ=255)
                  tGGCATTTAATTACCGCCGATGGTCAGGGTATTACTCTGGTGAATATTGGCGTGGGACCGTCAAATGCTaa                                                  <  1:1942932/71‑1 (MQ=255)
                    gCATTTAATTACCGCCGATGGTCAGGGTATTACTCTGGTGAATATTGGCGTGGGACCGTCAAATGCTaaaa                                                >  1:1646730/1‑71 (MQ=255)
                      aTTTAATTACCGCCGATGGTCAGGGTATTACTCTGGTGAATATTGGTGTGGGACCGTCAAATGCTAAAAcc                                              <  1:2580219/71‑1 (MQ=255)
                       tttAATTACCGCCGATGGTCAGGGTATTACTCTGGTGAATATTGGCGTGGGACCGTCAAATGCTAAAACCa                                             >  1:846796/1‑71 (MQ=255)
                         tAATTACCGCCGATGGTCAGGGTATTACTCTGGTGAATATTGGCGTGGGAc                                                               >  1:525989/1‑51 (MQ=255)
                         tAATTACCGCCGATGGTCAGGGTATTACTCTGGTGAATATTGGCGTGGGACCGTCAAATGCTAAAACCATc                                           >  1:1754350/1‑71 (MQ=255)
                         tAATTACCGCCGATGGTCAGGGTATTACTCTGGTGAATATTGGCGTGGGACCGTCAAATGCTAAAACCATc                                           >  1:2360309/1‑71 (MQ=255)
                         tAATTACCGCCGATGGTCAGGGTATTACTCTGGTGAATATTGGCGTGGGACCGTCAAATGCTAAAACCATc                                           >  1:2521079/1‑71 (MQ=255)
                                cgccgATGGTCAGGGTATTACTCTGGTGAATATTGGCGTGGGACCGTCAAATGCTAAAACCATCTGCGatc                                    <  1:1923728/71‑1 (MQ=255)
                                 gccgATGGTCAGGGTATTACTCTGGTGAATATTGGTGTGGGACCGTCAAATGCTAAAACCATCTGCGatca                                   >  1:809556/1‑71 (MQ=255)
                                 gccgATGGTCAGGGTATTACTCTGGTGAATATTGGTGTGGGACCGTCAAATGCTAAAACCATCTGCGatca                                   >  1:656633/1‑71 (MQ=255)
                                 gccgATGGTCAGGGTATTACTCTGGTGAATATTGGTGTGGGACCGTCAAATGCTAAAACCATCTGCGatca                                   >  1:1435702/1‑71 (MQ=255)
                                  ccgATGGTCAGGGTATTACTCTGGTGAATATTGGTGTGGGACCGTCAAATGCTAAAACCATCTGCGatcat                                  <  1:887845/71‑1 (MQ=255)
                                         tCAGGGTATTACTCTGGTGAATATTGGTGTGGGACCGTCAAATGCTAAAACCATCTGCGATCATCTGTCAg                           <  1:1591298/71‑1 (MQ=255)
                                         tCAGGGTATTACTCTGGTGAATATTGGCGTGGGACCGTCAAATGCTAAAACCATCTGCGATCATCTGGCAg                           >  1:2463562/1‑71 (MQ=255)
                                              gTATTACTCTGGTGAATATTGGCGTGGGACCGTCAAATGCTAAAACCATCTGCGatcatc                                 >  1:706323/1‑60 (MQ=255)
                                               tATTACTCTGGTGAATATTGGTGTGGGACCGTCaa                                                         <  1:2424380/35‑1 (MQ=255)
                                               tATTACTCTGGTGAATATTGGCGTGGGACCGTCAAATGCTAAAACCATCTGCg                                       <  1:1683330/53‑1 (MQ=255)
                                                 ttACTCTGGTGAATATTGGCGTGGGACCGTCAAATGCTAAAACCATCTGCGATCATCTGGCAGTGCTACg                    >  1:391359/1‑70 (MQ=255)
                                                 ttACTCTGGTGAATATTGGCGTGGGACCGTCAAATGCTAAAACCATCTGCGATCATCTGGCAGTGCTACGc                   <  1:396491/71‑1 (MQ=255)
                                                 ttACTCTGGTGAATATTGGCGTGGGACCGTCAAATGCTAAAACCATCTGCGATCATCTGGCAGTGCTACGc                   <  1:2360399/71‑1 (MQ=255)
                                                 ttACTCTGGTGAATATTGGCCTGGGACCGTCAAATGCTAAAACCATCTGCGATCATCTGGCAGTGCTACGc                   <  1:853775/71‑1 (MQ=255)
                                                   aCTCTGGTGAATATTGGTGTGGGACCGTCAAATGCTAAAACCATCTGCGATCATCTGGCa                            >  1:1675792/1‑60 (MQ=255)
                                                   aCTCTGGTGAATATTGGTGTGGGACCGTCAAATGCTAAAACCATCTGCGATCATCTGGCAGTGCTACGccc                 >  1:480530/1‑71 (MQ=255)
                                                   aCTCTGGTGAATATTGGTGTGGGACCGTCAAATGCTAAAACCATCTGCGATCATCTGGCAGTGCTACGccc                 >  1:2487830/1‑71 (MQ=255)
                                                   aCTCTGGTGAATATTGGTGTGGGACCGTCAAATGCTAAAACCATCTGCGATCATCTGGCAGTGCTACGccc                 >  1:1810888/1‑71 (MQ=255)
                                                   aCTCTGGTGAATATTGGCGTGGGACCGTCAAATGCTAAAACCATCTGCGATCATCTGGCAGTGCTACGcc                  <  1:1204273/70‑1 (MQ=255)
                                                    ctctGGTGAATATTGGCGTGGGACCGTCAAATGCTAAAACCATCTGCGATCATCTGGCAGTGCTACGCCCg                <  1:2509764/71‑1 (MQ=255)
                                                     tctGGTGAATATTGGTGTGGGACCGTCAAATGCTAAAACCATCTGc                                        <  1:1164709/46‑1 (MQ=255)
                                                       tGGTGAATATTGGCGTGGGACCGTCAAATGCTaaaa                                                >  1:1245201/1‑36 (MQ=255)
                                                       tGGTGAATATTGGCGTGGGACCGTCAAATGCTAAAACCAt                                            >  1:1528141/1‑40 (MQ=255)
                                                         gTGAATATTGGTGTGGGACCGTCAAATGCTAAAACCAt                                            >  1:2216043/1‑38 (MQ=255)
                                                         gTGAATATTGGTGTGGGACCGTCAAATGCTAAAACCATCTGCGATCATCTGGCAGTg                         >  1:976792/1‑57 (MQ=255)
                                                                ttGGCGTGGGACCGTCAAATGCTAAAACCATCTGCGATCATCTgg                              <  1:1158253/45‑1 (MQ=255)
                                                                  ggCGTGGGACCGTCAAATGCTAAAACCATCTGCGATCATCTGGCAGTGCTACGCCCGGATGTCTGGTtgat  >  1:1608804/1‑71 (MQ=255)
                                                                    cGTGGGACCGTCAAATGCTAAAACCATCTGCGATCATCTGGCAGTGCTACGCCCGGATGTCTGGTtgat  <  1:636239/69‑1 (MQ=255)
                                                                    |                                                                    
AAACATCAGATGCCAGCATGGCATTTAATTACCGCCGATGGTCAGGGTATTACTCTGGTGAATATTGGCGTGGGACCGTCAAATGCTAAAACCATCTGCGATCATCTGGCAGTGCTACGCCCGGATGTCTGGTTGAT  >  W3110S.gb/2057966‑2058102

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: