Predicted mutation | |||||||
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evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | W3110S.gb | 2,058,034 | C→T | 37.2% | G279G (GGC→GGT) | amn → | AMP nucleosidase |
Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | W3110S.gb | 2,058,034 | 0 | C | T | 37.2% | 41.0 / 43.1 | 43 | G279G (GGC→GGT) | amn | AMP nucleosidase |
Reads supporting (aligned to +/- strand): ref base C (18/9); new base T (10/6); total (28/15) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
AAACATCAGATGCCAGCATGGCATTTAATTACCGCCGATGGTCAGGGTATTACTCTGGTGAATATTGGCGTGGGACCGTCAAATGCTAAAACCATCTGCGATCATCTGGCAGTGCTACGCCCGGATGTCTGGTTGAT > W3110S.gb/2057966‑2058102 | aaaCATCAGATGCCAGCATGGCATTTAATTACCGCCGATGGTCAGGGTATTACTCTGGTGAATATTGGCGt > 1:2194027/1‑71 (MQ=255) cAGATGCCAGCATGGCATTTAATTACCGCCGATGGTCAGGGTATTACTCTGGTGAATATTGGTGTGGGAcc > 1:2461406/1‑71 (MQ=255) aGATGCCAGCATGGCATTTAATTACCGCCGATGGTCAGGGTATTACTCTGGTGAATATTGGCGTGGGACCg > 1:1657502/1‑71 (MQ=255) ccAGCATGGCATTTAATTACCGCCGATGGTCAGGGTATTACTCTGGTGAATATTGGTGTGGGACCGTCaaa < 1:1638490/71‑1 (MQ=255) ccAGCATGGCATTTAATTACCGCCGATGGTCAGGGTATTACTCTGGTGAATATTGGCGTGGGACCGTCaaa > 1:1006434/1‑71 (MQ=255) cAGCATGGCATTTAATTACCGCCGATGGTCAGGGTATTACTCTGGTGAATATTGGCGTGGGACCGTCaaa > 1:645799/1‑70 (MQ=255) cAGCATGGCATTTAATTACCGCCGATGGTCAGGGTATTACTCTGGTGAATATTGGCGTGGGACCGTCaaa > 1:1923364/1‑70 (MQ=255) cAGCATGGCATTTAATTACCGCCGATGGTCAGGGTATTACTCTGGTGAATATTGGCGTGGGACCGTCaaa > 1:1768251/1‑70 (MQ=255) tGGCATTTAATTACCGCCGATGGTCAGGGTATTACTCTGGTGAATATTGGc < 1:2538250/51‑1 (MQ=255) tGGCATTTAATTACCGCCGATGGTCAGGGTATTACTCTGGTGAATATTGGCGTGGGACCGTCAAATGCTaa < 1:1942932/71‑1 (MQ=255) gCATTTAATTACCGCCGATGGTCAGGGTATTACTCTGGTGAATATTGGCGTGGGACCGTCAAATGCTaaaa > 1:1646730/1‑71 (MQ=255) aTTTAATTACCGCCGATGGTCAGGGTATTACTCTGGTGAATATTGGTGTGGGACCGTCAAATGCTAAAAcc < 1:2580219/71‑1 (MQ=255) tttAATTACCGCCGATGGTCAGGGTATTACTCTGGTGAATATTGGCGTGGGACCGTCAAATGCTAAAACCa > 1:846796/1‑71 (MQ=255) tAATTACCGCCGATGGTCAGGGTATTACTCTGGTGAATATTGGCGTGGGAc > 1:525989/1‑51 (MQ=255) tAATTACCGCCGATGGTCAGGGTATTACTCTGGTGAATATTGGCGTGGGACCGTCAAATGCTAAAACCATc > 1:1754350/1‑71 (MQ=255) tAATTACCGCCGATGGTCAGGGTATTACTCTGGTGAATATTGGCGTGGGACCGTCAAATGCTAAAACCATc > 1:2360309/1‑71 (MQ=255) tAATTACCGCCGATGGTCAGGGTATTACTCTGGTGAATATTGGCGTGGGACCGTCAAATGCTAAAACCATc > 1:2521079/1‑71 (MQ=255) cgccgATGGTCAGGGTATTACTCTGGTGAATATTGGCGTGGGACCGTCAAATGCTAAAACCATCTGCGatc < 1:1923728/71‑1 (MQ=255) gccgATGGTCAGGGTATTACTCTGGTGAATATTGGTGTGGGACCGTCAAATGCTAAAACCATCTGCGatca > 1:809556/1‑71 (MQ=255) gccgATGGTCAGGGTATTACTCTGGTGAATATTGGTGTGGGACCGTCAAATGCTAAAACCATCTGCGatca > 1:656633/1‑71 (MQ=255) gccgATGGTCAGGGTATTACTCTGGTGAATATTGGTGTGGGACCGTCAAATGCTAAAACCATCTGCGatca > 1:1435702/1‑71 (MQ=255) ccgATGGTCAGGGTATTACTCTGGTGAATATTGGTGTGGGACCGTCAAATGCTAAAACCATCTGCGatcat < 1:887845/71‑1 (MQ=255) tCAGGGTATTACTCTGGTGAATATTGGTGTGGGACCGTCAAATGCTAAAACCATCTGCGATCATCTGTCAg < 1:1591298/71‑1 (MQ=255) tCAGGGTATTACTCTGGTGAATATTGGCGTGGGACCGTCAAATGCTAAAACCATCTGCGATCATCTGGCAg > 1:2463562/1‑71 (MQ=255) gTATTACTCTGGTGAATATTGGCGTGGGACCGTCAAATGCTAAAACCATCTGCGatcatc > 1:706323/1‑60 (MQ=255) tATTACTCTGGTGAATATTGGTGTGGGACCGTCaa < 1:2424380/35‑1 (MQ=255) tATTACTCTGGTGAATATTGGCGTGGGACCGTCAAATGCTAAAACCATCTGCg < 1:1683330/53‑1 (MQ=255) ttACTCTGGTGAATATTGGCGTGGGACCGTCAAATGCTAAAACCATCTGCGATCATCTGGCAGTGCTACg > 1:391359/1‑70 (MQ=255) ttACTCTGGTGAATATTGGCGTGGGACCGTCAAATGCTAAAACCATCTGCGATCATCTGGCAGTGCTACGc < 1:396491/71‑1 (MQ=255) ttACTCTGGTGAATATTGGCGTGGGACCGTCAAATGCTAAAACCATCTGCGATCATCTGGCAGTGCTACGc < 1:2360399/71‑1 (MQ=255) ttACTCTGGTGAATATTGGCCTGGGACCGTCAAATGCTAAAACCATCTGCGATCATCTGGCAGTGCTACGc < 1:853775/71‑1 (MQ=255) aCTCTGGTGAATATTGGTGTGGGACCGTCAAATGCTAAAACCATCTGCGATCATCTGGCa > 1:1675792/1‑60 (MQ=255) aCTCTGGTGAATATTGGTGTGGGACCGTCAAATGCTAAAACCATCTGCGATCATCTGGCAGTGCTACGccc > 1:480530/1‑71 (MQ=255) aCTCTGGTGAATATTGGTGTGGGACCGTCAAATGCTAAAACCATCTGCGATCATCTGGCAGTGCTACGccc > 1:2487830/1‑71 (MQ=255) aCTCTGGTGAATATTGGTGTGGGACCGTCAAATGCTAAAACCATCTGCGATCATCTGGCAGTGCTACGccc > 1:1810888/1‑71 (MQ=255) aCTCTGGTGAATATTGGCGTGGGACCGTCAAATGCTAAAACCATCTGCGATCATCTGGCAGTGCTACGcc < 1:1204273/70‑1 (MQ=255) ctctGGTGAATATTGGCGTGGGACCGTCAAATGCTAAAACCATCTGCGATCATCTGGCAGTGCTACGCCCg < 1:2509764/71‑1 (MQ=255) tctGGTGAATATTGGTGTGGGACCGTCAAATGCTAAAACCATCTGc < 1:1164709/46‑1 (MQ=255) tGGTGAATATTGGCGTGGGACCGTCAAATGCTaaaa > 1:1245201/1‑36 (MQ=255) tGGTGAATATTGGCGTGGGACCGTCAAATGCTAAAACCAt > 1:1528141/1‑40 (MQ=255) gTGAATATTGGTGTGGGACCGTCAAATGCTAAAACCAt > 1:2216043/1‑38 (MQ=255) gTGAATATTGGTGTGGGACCGTCAAATGCTAAAACCATCTGCGATCATCTGGCAGTg > 1:976792/1‑57 (MQ=255) ttGGCGTGGGACCGTCAAATGCTAAAACCATCTGCGATCATCTgg < 1:1158253/45‑1 (MQ=255) ggCGTGGGACCGTCAAATGCTAAAACCATCTGCGATCATCTGGCAGTGCTACGCCCGGATGTCTGGTtgat > 1:1608804/1‑71 (MQ=255) cGTGGGACCGTCAAATGCTAAAACCATCTGCGATCATCTGGCAGTGCTACGCCCGGATGTCTGGTtgat < 1:636239/69‑1 (MQ=255) | AAACATCAGATGCCAGCATGGCATTTAATTACCGCCGATGGTCAGGGTATTACTCTGGTGAATATTGGCGTGGGACCGTCAAATGCTAAAACCATCTGCGATCATCTGGCAGTGCTACGCCCGGATGTCTGGTTGAT > W3110S.gb/2057966‑2058102 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |