| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 2,286,501 | C→A | 48.2% | A261S (GCG→TCG) | yejK ← | nucleotide associated protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,286,501 | 0 | C | A | 48.2% | 20.7 / 29.6 | 29 | A261S (GCG→TCG) | yejK | nucleotide associated protein |
| Reads supporting (aligned to +/- strand): ref base C (9/6); new base A (6/8); total (15/14) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.66e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.73e-01 | |||||||||||
CGTCAACTGACGCAGTGTGCTACGGTCTGCCGGGAAGCTCTCTTCCAGTTCGTAGCCTTTTTCAGCCGCAAACTCCGTGAAGCTCACTTCGCTTACGCCTGCCAGCTCTTTCGACAGCGATTTCAGTTCG > W3110S.gb/2286433‑2286562 | cGTCAACTGACGCAGTGTGCTACGGTCTGCCGGGAAGCTCTCTTCCAGTTCGTAGCCTTTTTCAGCCGCa > 1:1048443/1‑70 (MQ=255) aaCTGACGCAGTGTGCTACGGTCTGCCGGGAAGCTCTCTTCCAGTTCGTAGCCTTTTTAAGCCGCAAAc > 1:1618365/1‑69 (MQ=255) cAGTGTGCTACGGTCTGCCGGGAAGCTCTCTTCCAGTTCGTAGCCTTTTTCAGCCGAAAACTCCGTGAAGc < 1:1956215/71‑1 (MQ=255) gtgtGCTACGGTCTGCCGGGAAGCTCTCTTCCAGTTCGTAGCCTTTTTCAGCCGAAAACTcc > 1:776328/1‑62 (MQ=255) tgCTACGGTCTGCCGGGAAGCTCTCTTCCAGTTCGTAGCCTTTTTCAGCCGAAAACTCCGTGAAGCTCACt < 1:439685/71‑1 (MQ=255) cGGTCTGCCGGGAAGCTCTCTTCCAGTTCGTAGCCTTTTTCAGCCGCAAACTCCGTGAAGCTCACTTCg < 1:1430597/69‑1 (MQ=255) cGGTCTGCCGGGAAGCTCTCTTCCAGTTCGTAGCCTTTTTCAGCCGAAAACTCCGTGAAGCTCACTTCGCt > 1:819273/1‑71 (MQ=255) cGGTCTGCCGGGAAGCTCTCTTCCAGTTCGTAGCCTTTTTCAGCCGAAAACTCCGTGAAGCTCACTTCGCt < 1:2338627/71‑1 (MQ=255) cGGTCTGCCGGGAAGCTCTCTTCCAGTTCGTAGCCTTTTTCAGCCGAAAACTCCGTGAAGCTCACTTCGCt > 1:816179/1‑71 (MQ=255) ggTCTGCCGGGAAGCTCTCTTCCAGTTCGTAGCCTTTTTCAGCCGCAAACTCCGTGAAGCTCACTTCGCt < 1:563804/70‑1 (MQ=255) gTCTGCCGGGAAGCTCTCTTCCAGTTCGTAGCCTTTTTCAGCCGCAAACTCCGTGAAGCTCACTTCGCTTa > 1:2573534/1‑71 (MQ=255) tCTGCTGGGAAGCTCTCTTCCAGTTCGTAGCCTTTTTCAGCCGaaaa > 1:1269437/1‑47 (MQ=39) tCTGCCGGGAAGCTCTCTTCCAGTTCGTAGCCTTTTTCAGCCGAAAACTCCGTGAAGCTCACTTCGCTTAc < 1:989100/71‑1 (MQ=255) tCTGCCGGGAAGCTCTCTTCCAGTTCGTAGCCTTTTTCAGCCGAAAACTCCGTGAAGCTCACTTCGCTTAc < 1:193598/71‑1 (MQ=255) tGCCGGGAAGCTCTCTTCCAGTTCGTAGCCTTTTTCAGCCGCAAACTCCGTGAAGCTCACtt > 1:1958043/1‑62 (MQ=255) ccGGGAAGCTCTCTTCCAGTTCGTAGCCTTTTTCAGCCGCAAACTCCGTGAAGCTCACTTCGCTTACGCCt < 1:738519/71‑1 (MQ=255) gCTCTCTTCCAGTTCGTAGCCTTTTTCAGCCGAAAACTCCGTGAAGCTCACTTCGCTTACGCCTGCCAGc < 1:1723963/70‑1 (MQ=255) ctctctTCCAGTTCGTAGCCTTTTTCAGCCGaaaa > 1:504904/1‑35 (MQ=255) tctTCCAGTTCGTAGCCTTTTTCAGCCGCAAACTCCGTGAAGCTCACTTCGCTTACGCCTGCCAGCTCttt > 1:1469714/1‑71 (MQ=255) ctTCCAGTTCGTAGCCTTTTTCAGCCGCAAACTCCGTGAAg > 1:68338/1‑41 (MQ=255) cGTAGCCTTTTTCAGCCGCAAACTCCGTGAAGCTCACTTCGCt < 1:1147680/43‑1 (MQ=255) gTAGCCTTTTTCAGCCGCAAACTCCGTGAAGCTCACTTCGCTTACGCCTGCCAGCTCTTTCGACAGCGAtt > 1:799720/1‑71 (MQ=255) gCCTTTTTCAGCCGCAAACTCCGTGAAGCTCACTTCGCTTACGCCTGCCAGCTCTTTCGACAGCGAttt > 1:912457/1‑69 (MQ=255) gCCTTTTTCAGCCGAAAACTCCGTGAAGCTCACTTCGCTTACGCCTGCCAGCTCTTTCGACAGCGAttt > 1:26066/1‑69 (MQ=255) ccTTTTTCAGCCGCAAACTCCGTGAAGCTCACTTCGCTTACGCCTGCCAGCTCTTTCGACAGCGATTTCAg < 1:984280/71‑1 (MQ=255) cTTTTTCAGCCGAAAACTCCGTGAAGCTCACTTCGCTTACGCCTGCCAGct < 1:477845/51‑1 (MQ=255) cTTTTTCAGCCGAAAACTCCGTGAAGCTCACTTCGCTTACGCCTGCCAGct < 1:928581/51‑1 (MQ=255) tttCAGCCGCAAACTCCGTGAAGCTCACTTCGCTTACGCCTGCCAGCTCTTTCGACAGCGATTTCAGTTc > 1:185587/1‑70 (MQ=255) tttCAGCCGAAAACTCCGTGAAGCTCACTTCGCTTa > 1:624439/1‑36 (MQ=255) tttCAGCCGAAAACTCCGTGAAGCTCACTTCGCTTACGCCTGCCAGCTCTTTCGACAGCGATTTCAGTTc > 1:2249842/1‑70 (MQ=255) ttCAGCCGCAAACTCCGTGAAGCTCACTTCGCTTACGCCTGCCAGCTCTTTCGACAGCGATTTCAGTTCg < 1:2359356/70‑1 (MQ=255) | CGTCAACTGACGCAGTGTGCTACGGTCTGCCGGGAAGCTCTCTTCCAGTTCGTAGCCTTTTTCAGCCGCAAACTCCGTGAAGCTCACTTCGCTTACGCCTGCCAGCTCTTTCGACAGCGATTTCAGTTCG > W3110S.gb/2286433‑2286562 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |